Saccharothrix espanaensis (DSM 44229, ATCC 51144, IFO 15066)

Cell morphology

Multimedia

• @ref:	11491
  multimedia content:	DSM_44229-1.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44229-1.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

• @ref:	11491
  multimedia content:	DSM_44229.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44229.jpg
  caption:	Medium 65 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
11491	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

Oxygen tolerance

Spore formation

Compound production

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	27613 ChEBI	amygdalin	-	fermentation	from API 20E
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68368	17634 ChEBI	D-glucose	-	fermentation	from API 20E
68368	16899 ChEBI	D-mannitol	-	fermentation	from API 20E
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68368	30849 ChEBI	L-arabinose	-	fermentation	from API 20E
68368	62345 ChEBI	L-rhamnose	-	fermentation	from API 20E
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
68368	17632 ChEBI	nitrate	-	reduction	from API 20E
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
68368	17997 ChEBI	dinitrogen		from API 20E
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E
68368	16301 ChEBI	nitrite		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68368	15688 ChEBI	acetoin	+	from API 20E
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	acetate fermentation	100	4 of 4
66794	starch degradation	100	10 of 10
66794	biotin biosynthesis	100	4 of 4
66794	D-cycloserine biosynthesis	100	5 of 5
66794	chorismate metabolism	100	9 of 9
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	adipate degradation	100	2 of 2
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	vitamin K metabolism	100	5 of 5
66794	glycine betaine biosynthesis	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	grixazone biosynthesis	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	Entner Doudoroff pathway	100	10 of 10
66794	aspartate and asparagine metabolism	100	9 of 9
66794	palmitate biosynthesis	100	22 of 22
66794	ethanol fermentation	100	2 of 2
66794	threonine metabolism	100	10 of 10
66794	ppGpp biosynthesis	100	4 of 4
66794	dTDPLrhamnose biosynthesis	100	8 of 8
66794	methylglyoxal degradation	100	5 of 5
66794	enterobactin biosynthesis	100	3 of 3
66794	butanoate fermentation	100	4 of 4
66794	resorcinol degradation	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	cardiolipin biosynthesis	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	cellulose degradation	100	5 of 5
66794	suberin monomers biosynthesis	100	2 of 2
66794	taurine degradation	100	1 of 1
66794	aerobactin biosynthesis	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	isoleucine metabolism	100	8 of 8
66794	daunorubicin biosynthesis	100	9 of 9
66794	sulfopterin metabolism	100	4 of 4
66794	glycogen metabolism	100	5 of 5
66794	cyanate degradation	100	3 of 3
66794	NAD metabolism	94.44	17 of 18
66794	flavin biosynthesis	93.33	14 of 15
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	leucine metabolism	92.31	12 of 13
66794	4-hydroxyphenylacetate degradation	90	9 of 10
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	4-hydroxymandelate degradation	88.89	8 of 9
66794	purine metabolism	88.3	83 of 94
66794	vitamin B12 metabolism	88.24	30 of 34
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	glutathione metabolism	85.71	12 of 14
66794	glutamate and glutamine metabolism	85.71	24 of 28
66794	heme metabolism	85.71	12 of 14
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	propanol degradation	85.71	6 of 7
66794	citric acid cycle	85.71	12 of 14
66794	aclacinomycin biosynthesis	85.71	6 of 7
66794	methionine metabolism	84.62	22 of 26
66794	tryptophan metabolism	84.21	32 of 38
66794	alanine metabolism	82.76	24 of 29
66794	glycolysis	82.35	14 of 17
66794	pentose phosphate pathway	81.82	9 of 11
66794	proline metabolism	81.82	9 of 11
66794	factor 420 biosynthesis	80	4 of 5
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	3-chlorocatechol degradation	80	4 of 5
66794	pyrimidine metabolism	80	36 of 45
66794	creatinine degradation	80	4 of 5
66794	histidine metabolism	79.31	23 of 29
66794	photosynthesis	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	allantoin degradation	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	urea cycle	76.92	10 of 13
66794	non-pathway related	76.32	29 of 38
66794	gluconeogenesis	75	6 of 8
66794	degradation of aromatic, nitrogen containing compounds	75	9 of 12
66794	glycogen biosynthesis	75	3 of 4
66794	arginine metabolism	75	18 of 24
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	lysine metabolism	73.81	31 of 42
66794	carotenoid biosynthesis	72.73	16 of 22
66794	metabolism of disaccharids	72.73	8 of 11
66794	tyrosine metabolism	71.43	10 of 14
66794	polyamine pathway	69.57	16 of 23
66794	oxidative phosphorylation	69.23	63 of 91
66794	isoprenoid biosynthesis	69.23	18 of 26
66794	androgen and estrogen metabolism	68.75	11 of 16
66794	octane oxidation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	3-phenylpropionate degradation	66.67	10 of 15
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	IAA biosynthesis	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	phenol degradation	65	13 of 20
66794	lipid metabolism	64.52	20 of 31
66794	d-xylose degradation	63.64	7 of 11
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	degradation of sugar alcohols	62.5	10 of 16
66794	ketogluconate metabolism	62.5	5 of 8
66794	sulfate reduction	61.54	8 of 13
66794	phenylpropanoid biosynthesis	61.54	8 of 13
66794	cysteine metabolism	61.11	11 of 18
66794	lipoate biosynthesis	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	vitamin B6 metabolism	54.55	6 of 11
66794	degradation of pentoses	53.57	15 of 28
66794	kanosamine biosynthesis II	50	1 of 2
66794	catecholamine biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	vitamin E metabolism	50	2 of 4
66794	CMP-KDO biosynthesis	50	2 of 4
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	toluene degradation	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	myo-inositol biosynthesis	50	5 of 10
66794	cyclohexanol degradation	50	2 of 4
66794	coenzyme M biosynthesis	50	5 of 10
66794	phenylmercury acetate degradation	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	sphingosine metabolism	50	3 of 6
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	ascorbate metabolism	45.45	10 of 22
66794	cholesterol biosynthesis	45.45	5 of 11
66794	nitrate assimilation	44.44	4 of 9
66794	lipid A biosynthesis	44.44	4 of 9
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	bacilysin biosynthesis	40	2 of 5
66794	elloramycin biosynthesis	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	arachidonic acid metabolism	38.89	7 of 18
66794	chlorophyll metabolism	38.89	7 of 18
66794	carnitine metabolism	37.5	3 of 8
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	methane metabolism	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	degradation of sugar acids	32	8 of 25
66794	mevalonate metabolism	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	alginate biosynthesis	25	1 of 4
66794	methanogenesis from CO2	25	3 of 12

API 20E

API zym

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence AF114807 (>99% sequence identity) for Saccharothrix from Microbeatlas

Aquatic Samples	49
Soil Samples	337
Animal Samples	83
Plant Samples	38
Total Samples	507

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM32870v1 assembly for Saccharothrix espanaensis DSM 44229 type strain: DSM 44229	complete	GCA_000328705	1179773.3	2540341184	1179773	98.89

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Saccharothrix espanaensis strain NRRL 15764 16S ribosomal RNA gene, partial sequence	AF114807	1510		103731

GC content

Predictions

Literature

Title	Authors	Journal	DOI	Year
Evaluation of antimicrobial efficacy against spoilage microorganisms and impact on beverage color and turbidity of commercial preservatives derived from edible mushrooms.	Galasong Y, Charles-Vegdahl A, Worobo RW.	Int J Food Microbiol	10.1016/j.ijfoodmicro.2025.111130	2025
Halovirs I-K, antibacterial and cytotoxic lipopeptaibols from the plant pathogenic fungus Paramyrothecium roridum NRRL 2183.	Xiao D, Zhang M, Wu P, Li T, Li W, Zhang L, Yue Q, Chen X, Wei X, Xu Y, Wang C.	J Antibiot (Tokyo)	10.1038/s41429-022-00517-7	2022
Promiscuity of lanthipeptide enzymes: new challenges and applications.	Garcia-Ausencio C, Guzman-Chavez F, Rodriguez-Sanoja R, Sanchez S.	World J Microbiol Biotechnol	10.1007/s11274-025-04505-5	2025
Actinomycetes as Producers of Biologically Active Terpenoids: Current Trends and Patents.	Tarasova EV, Luchnikova NA, Grishko VV, Ivshina IB.	Pharmaceuticals (Basel)	10.3390/ph16060872	2023
Characterization of LnmO as a pathway-specific Crp/Fnr-type positive regulator for leinamycin biosynthesis in Streptomyces atroolivaceus and its application for titer improvement.	Huang Y, Yang D, Pan G, Tang GL, Shen B.	Appl Microbiol Biotechnol	10.1007/s00253-016-7864-2	2016
Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.	Strobel T, Schmidt Y, Linnenbrink A, Luzhetskyy A, Luzhetska M, Taguchi T, Brotz E, Paululat T, Stasevych M, Stanko O, Novikov V, Bechthold A.	Appl Environ Microbiol	10.1128/aem.01652-13	2013
Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for genetic screening techniques.	Petrickova K, Petrickova K, Chronakova A, Zelenka T, Chrudimsky T, Pospisil S, Petricek M, Kristufek V.	Front Microbiol	10.3389/fmicb.2015.00814	2015
Critical Role of Zur and SmtB in Zinc Homeostasis of Mycobacterium smegmatis.	Goethe E, Laarmann K, Luhrs J, Jarek M, Meens J, Lewin A, Goethe R.	mSystems	10.1128/msystems.00880-19	2020
Mechanistic investigations on six bacterial terpene cyclases.	Rabe P, Schmitz T, Dickschat JS.	Beilstein J Org Chem	10.3762/bjoc.12.173	2016
Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces.	Bai C, Zhang Y, Zhao X, Hu Y, Xiang S, Miao J, Lou C, Zhang L.	Proc Natl Acad Sci U S A	10.1073/pnas.1511027112	2015
Genome neighborhood network reveals insights into enediyne biosynthesis and facilitates prediction and prioritization for discovery.	Rudolf JD, Yan X, Shen B.	J Ind Microbiol Biotechnol	10.1007/s10295-015-1671-0	2016
Biosynthesis of akaeolide and lorneic acids and annotation of type I polyketide synthase gene clusters in the genome of Streptomyces sp. NPS554.	Zhou T, Komaki H, Ichikawa N, Hosoyama A, Sato S, Igarashi Y.	Mar Drugs	10.3390/md13010581	2015
Chemometrics and genome mining reveal an unprecedented family of sugar acid-containing fungal nonribosomal cyclodepsipeptides.	Wang C, Xiao D, Dun B, Yin M, Tsega AS, Xie L, Li W, Yue Q, Wang S, Gao H, Lin M, Zhang L, Molnar I, Xu Y.	Proc Natl Acad Sci U S A	10.1073/pnas.2123379119	2022
Complete genome sequence of producer of the glycopeptide antibiotic Aculeximycin Kutzneria albida DSM 43870T, a representative of minor genus of Pseudonocardiaceae.	Rebets Y, Tokovenko B, Lushchyk I, Ruckert C, Zaburannyi N, Bechthold A, Kalinowski J, Luzhetskyy A.	BMC Genomics	10.1186/1471-2164-15-885	2014
Identification and characterization of andalusicin: N-terminally dimethylated class III lantibiotic from Bacillus thuringiensis sv. andalousiensis.	Grigoreva A, Andreeva J, Bikmetov D, Rusanova A, Serebryakova M, Garcia AH, Slonova D, Nair SK, Lippens G, Severinov K, Dubiley S.	iScience	10.1016/j.isci.2021.102480	2021
Polysaccharide Degradation Capability of Actinomycetales Soil Isolates from a Semiarid Grassland of the Colorado Plateau.	Yeager CM, Gallegos-Graves V, Dunbar J, Hesse CN, Daligault H, Kuske CR.	Appl Environ Microbiol	10.1128/aem.03020-16	2017
Genome-based analysis of non-ribosomal peptide synthetase and type-I polyketide synthase gene clusters in all type strains of the genus Herbidospora.	Komaki H, Ichikawa N, Oguchi A, Hamada M, Tamura T, Fujita N.	BMC Res Notes	10.1186/s13104-015-1526-9	2015
Genes and enzymes involved in caffeic acid biosynthesis in the actinomycete Saccharothrix espanaensis.	Berner M, Krug D, Bihlmaier C, Vente A, Muller R, Bechthold A.	J Bacteriol	10.1128/jb.188.7.2666-2673.2006	2006
Identification of a cyclosporine-specific P450 hydroxylase gene through targeted cytochrome P450 complement (CYPome) disruption in Sebekia benihana.	Lee MJ, Kim HB, Yoon YJ, Han K, Kim ES.	Appl Environ Microbiol	10.1128/aem.03722-12	2013
Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources.	Ni J, Tao F, Du H, Xu P.	Sci Rep	10.1038/srep13670	2015
Designing and Implementing an Assay for the Detection of Rare and Divergent NRPS and PKS Clones in European, Antarctic and Cuban Soils.	Amos GC, Borsetto C, Laskaris P, Krsek M, Berry AE, Newsham KK, Calvo-Bado L, Pearce DA, Vallin C, Wellington EM.	PLoS One	10.1371/journal.pone.0138327	2015
Construction of a chimeric biosynthetic pathway for the de novo biosynthesis of rosmarinic acid in Escherichia coli.	Bloch SE, Schmidt-Dannert C.	Chembiochem	10.1002/cbic.201402275	2014
Inteins as indicators of gene flow in the halobacteria.	Soucy SM, Fullmer MS, Papke RT, Gogarten JP.	Front Microbiol	10.3389/fmicb.2014.00299	2014
Synthetic pathways to create asymmetric center at C1 position of 1-substituted-tetrahydro-beta-carbolines - a review.	Asif MMA, Lisa SR, Qais N.	RSC Adv	10.1039/d4ra05961a	2024
Introduction of chirality at C1 position of 1-substituted-3,4-dihydroisoquinoline by its enantioselective reduction: synthesis of chiral 1-substituted-1,2,3,4-tetrahydroisoquinoline - a review.	Asif MMA, Lisa SR, Qais N.	RSC Adv	10.1039/d3ra01413d	2023
Mining of Microbial Genomes for the Novel Sources of Nitrilases.	Sharma N, Thakur N, Raj T, Savitri, Bhalla TC.	Biomed Res Int	10.1155/2017/7039245	2017
Sequence-Based In-silico Discovery, Characterisation, and Biocatalytic Application of a Set of Imine Reductases.	Velikogne S, Resch V, Dertnig C, Schrittwieser JH, Kroutil W.	ChemCatChem	10.1002/cctc.201800607	2018
The role of biocatalysis in the asymmetric synthesis of alkaloids - an update.	Cigan E, Eggbauer B, Schrittwieser JH, Kroutil W.	RSC Adv	10.1039/d1ra04181a	2021
Saccharomicins, novel heptadecaglycoside antibiotics produced by Saccharothrix espanaensis: antibacterial and mechanistic activities.	Singh MP, Petersen PJ, Weiss WJ, Kong F, Greenstein M.	Antimicrob Agents Chemother	10.1128/aac.44.8.2154-2159.2000	2000
De Novo Artificial Biosynthesis of 3-Hydroxyphloretin in Escherichia coli.	Won J, Lee B, Park B, Ahn J, Hwang BY, Jang JH, Hong YS.	J Agric Food Chem	10.1021/acs.jafc.5c01962	2025
Caffeic acid production from glucose using metabolically engineered Escherichia coli.	Sakae K, Nonaka D, Kishida M, Hirata Y, Fujiwara R, Kondo A, Noda S, Tanaka T.	Enzyme Microb Technol	10.1016/j.enzmictec.2023.110193	2023
Biosynthesis of Rhamnosylated Anthraquinones in Escherichia coli.	Nguyen TTH, Sin HJ, Pandey RP, Jung HJ, Liou K, Sohng JK.	J Microbiol Biotechnol	10.4014/jmb.1911.11047	2020
Ferulic acid production by metabolically engineered Escherichia coli.	Lv H, Zhang Y, Shao J, Liu H, Wang Y.	Bioresour Bioprocess	10.1186/s40643-021-00423-0	2021
A robust high-throughput screening system to assess bacterial tyrosine ammonia lyase activity in the context of tyrosine inherited metabolic disorders.	Nulmans I, Laga CA, Salvi NS, Desmet L, Lequeue S, Neuckermans J, Schwaneberg U, De Kock J.	Sci Rep	10.1038/s41598-024-72360-9	2024
The Rare Actinobacterium Crossiella sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi.	Gonzalez-Pimentel JL, Dominguez-Monino I, Jurado V, Laiz L, Caldeira AT, Saiz-Jimenez C.	Microorganisms	10.3390/microorganisms10081575	2022
Production of bioactive hydroxyflavones by using monooxygenase from Saccharothrix espanaensis.	Lee H, Kim BG, Ahn JH.	J Biotechnol	10.1016/j.jbiotec.2014.02.002	2014
Evaluating the Antagonistic Potential of Actinomycete Strains Isolated From Sudan's Soils Against Phytophthora infestans.	Abdelrahman O, Yagi S, El Siddig M, El Hussein A, Germanier F, De Vrieze M, L'Haridon F, Weisskopf L.	Front Microbiol	10.3389/fmicb.2022.827824	2022
Production of curcuminoids from tyrosine by a metabolically engineered Escherichia coli using caffeic acid as an intermediate.	Rodrigues JL, Araujo RG, Prather KL, Kluskens LD, Rodrigues LR.	Biotechnol J	10.1002/biot.201400637	2015
Heterologous production of caffeic acid from tyrosine in Escherichia coli.	Rodrigues JL, Araujo RG, Prather KL, Kluskens LD, Rodrigues LR.	Enzyme Microb Technol	10.1016/j.enzmictec.2015.01.001	2015
Challenges in the Heterologous Production of Furanocoumarins in Escherichia coli.	Rodrigues JL, Gomes D, Rodrigues LR.	Molecules	10.3390/molecules27217230	2022
Targeted Genome Mining-From Compound Discovery to Biosynthetic Pathway Elucidation.	Gummerlich N, Rebets Y, Paulus C, Zapp J, Luzhetskyy A.	Microorganisms	10.3390/microorganisms8122034	2020
Computational design of an imine reductase: mechanism-guided stereoselectivity reversion and interface stabilization.	Wu K, Yan J, Liu Q, Wang X, Wu P, Cao Y, Lu X, Xu Y, Huang J, Shao L.	Chem Sci	10.1039/d3sc04636b	2024
Microbial Biosynthesis of Chrysazin Derivatives in Recombinant Escherichia coli and Their Biological Activities.	Poudel PB, Dhakal D, Magar RT, Sohng JK.	Molecules	10.3390/molecules27175554	2022
Structure and Candidate Biosynthetic Gene Cluster of a Manumycin-Type Metabolite from Salinispora pacifica.	Castro-Falcon G, Creamer KE, Chase AB, Kim MC, Sweeney D, Glukhov E, Fenical W, Jensen PR.	J Nat Prod	10.1021/acs.jnatprod.1c01117	2022
Discovery of Novel Tyrosine Ammonia Lyases for the Enzymatic Synthesis of p-Coumaric Acid.	Brack Y, Sun C, Yi D, Bornscheuer UT.	Chembiochem	10.1002/cbic.202200062	2022
Evolution-aided engineering of plant specialized metabolism.	Irfan M, Chavez B, Rizzo P, D'Auria JC, Moghe GD.	aBIOTECH	10.1007/s42994-021-00052-3	2021
Actinomycetes-derived imine reductases with a preference towards bulky amine substrates.	Zhang J, Li X, Chen R, Tan X, Liu X, Ma Y, Zhu F, An C, Wei G, Yao Y, Yang L, Zhang P, Wu Q, Sun Z, Wang BG, Gao SS, Cui C.	Commun Chem	10.1038/s42004-022-00743-y	2022
Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli.	Choi O, Wu CZ, Kang SY, Ahn JS, Uhm TB, Hong YS.	J Ind Microbiol Biotechnol	10.1007/s10295-011-0954-3	2011
Altering the Regioselectivity of Cytochrome P450 BM3 Variant M13 toward Genistein through Protein Engineering and Variation of Reaction Conditions.	Hong LL, Kong JQ.	ACS Omega	10.1021/acsomega.0c05088	2020
Crystal Structure and Biochemical Analysis of a Cytochrome P450 CYP101D5 from Sphingomonas echinoides.	Subedi P, Do H, Lee JH, Oh TJ.	Int J Mol Sci	10.3390/ijms232113317	2022
Heterologous caffeic acid biosynthesis in Escherichia coli is affected by choice of tyrosine ammonia lyase and redox partners for bacterial Cytochrome P450.	Haslinger K, Prather KLJ.	Microb Cell Fact	10.1186/s12934-020-01300-9	2020
Functional Characterization of a Regiospecific Sugar-O-Methyltransferase from Nocardia.	Poudel PB, Pandey RP, Dhakal D, Kim TS, Nguyen TTH, Jung HJ, Shin HJ, Timalsina B, Sohng JK.	Appl Environ Microbiol	10.1128/aem.00754-22	2022
Production of phenylacetyl-homoserine lactone analogs by artificial biosynthetic pathway in Escherichia coli.	Kang SY, Lee JK, Jang JH, Hwang BY, Hong YS.	Microb Cell Fact	10.1186/s12934-015-0379-1	2015
Glucose metabolism and glycosylation link the gut microbiota to autoimmune diseases.	Wang L, Xu H, Yang H, Zhou J, Zhao L, Zhang F.	Front Immunol	10.3389/fimmu.2022.952398	2022
Developing Multi-Copy Chromosomal Integration Strategies for Heterologous Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae.	Qi H, Yu L, Li Y, Cai M, He J, Liu J, Hao L, Xu H, Qiao M.	Front Microbiol	10.3389/fmicb.2022.851706	2022
Highly Active and Specific Tyrosine Ammonia-Lyases from Diverse Origins Enable Enhanced Production of Aromatic Compounds in Bacteria and Saccharomyces cerevisiae.	Jendresen CB, Stahlhut SG, Li M, Gaspar P, Siedler S, Forster J, Maury J, Borodina I, Nielsen AT.	Appl Environ Microbiol	10.1128/aem.00405-15	2015
Microbial Production of Melanin Pigments from Caffeic Acid and L-tyrosine Using Streptomyces glaucescens and FCS-ECH-Expressing Escherichia coli.	Ahn SY, Jang S, Sudheer PDVN, Choi KY.	Int J Mol Sci	10.3390/ijms22052413	2021
Genetically engineering Synechocystis sp. Pasteur Culture Collection 6803 for the sustainable production of the plant secondary metabolite p-coumaric acid.	Xue Y, Zhang Y, Cheng D, Daddy S, He Q.	Proc Natl Acad Sci U S A	10.1073/pnas.1323725111	2014
De novo biosynthesis of pterostilbene in an Escherichia coli strain using a new resveratrol O-methyltransferase from Arabidopsis.	Heo KT, Kang SY, Hong YS.	Microb Cell Fact	10.1186/s12934-017-0644-6	2017
Biosynthesis of methylated resveratrol analogs through the construction of an artificial biosynthetic pathway in E. coli.	Kang SY, Lee JK, Choi O, Kim CY, Jang JH, Hwang BY, Hong YS.	BMC Biotechnol	10.1186/1472-6750-14-67	2014
Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering.	Yan ZB, Liang JL, Niu FX, Shen YP, Liu JZ.	Front Microbiol	10.3389/fmicb.2021.710405	2021
A Combinatorial Approach to Optimize the Production of Curcuminoids From Tyrosine in Escherichia coli.	Rodrigues JL, Gomes D, Rodrigues LR.	Front Bioeng Biotechnol	10.3389/fbioe.2020.00059	2020
Biological synthesis of coumarins in Escherichia coli.	Yang SM, Shim GY, Kim BG, Ahn JH.	Microb Cell Fact	10.1186/s12934-015-0248-y	2015
Engineering Escherichia coli towards de novo production of gatekeeper (2S)-flavanones: naringenin, pinocembrin, eriodictyol and homoeriodictyol.	Dunstan MS, Robinson CJ, Jervis AJ, Yan C, Carbonell P, Hollywood KA, Currin A, Swainston N, Feuvre RL, Micklefield J, Faulon JL, Breitling R, Turner N, Takano E, Scrutton NS.	Synth Biol (Oxf)	10.1093/synbio/ysaa012	2020
SACE_0012, a TetR-family transcriptional regulator, affects the morphogenesis of Saccharopolyspora erythraea.	Yin X, Xu X, Wu H, Yuan L, Huang X, Zhang B.	Curr Microbiol	10.1007/s00284-013-0410-x	2013
Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains - An Unusual Secondary Metabolite with Various Properties.	Greule A, Marolt M, Deubel D, Peintner I, Zhang S, Jessen-Trefzer C, De Ford C, Burschel S, Li SM, Friedrich T, Merfort I, Ludeke S, Bisel P, Muller M, Paululat T, Bechthold A.	Front Microbiol	10.3389/fmicb.2017.00221	2017
Initiation of the flexirubin biosynthesis in Chitinophaga pinensis.	Schoner TA, Fuchs SW, Schonau C, Bode HB.	Microb Biotechnol	10.1111/1751-7915.12110	2014
Artificial biosynthesis of phenylpropanoic acids in a tyrosine overproducing Escherichia coli strain.	Kang SY, Choi O, Lee JK, Hwang BY, Uhm TB, Hong YS.	Microb Cell Fact	10.1186/1475-2859-11-153	2012
Bacterial Quorum-Sensing Molecules as Promising Natural Inhibitors of Candida albicans Virulence Dimorphism: An In Silico and In Vitro Study.	Jothi R, Hari Prasath N, Gowrishankar S, Pandian SK.	Front Cell Infect Microbiol	10.3389/fcimb.2021.781790	2021
Synthesis of umbelliferone derivatives in Escherichia coli and their biological activities.	Chu LL, Pandey RP, Lim HN, Jung HJ, Thuan NH, Kim TS, Sohng JK.	J Biol Eng	10.1186/s13036-017-0056-5	2017
Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain.	Kang SY, Choi O, Lee JK, Ahn JO, Ahn JS, Hwang BY, Hong YS.	Microb Cell Fact	10.1186/s12934-015-0268-7	2015
C-S bond cleavage by a polyketide synthase domain.	Ma M, Lohman JR, Liu T, Shen B.	Proc Natl Acad Sci U S A	10.1073/pnas.1508437112	2015
Dissecting and engineering of the TetR family regulator SACE_7301 for enhanced erythromycin production in Saccharopolyspora erythraea.	Wu H, Chen M, Mao Y, Li W, Liu J, Huang X, Zhou Y, Ye BC, Zhang L, Weaver DT, Zhang B.	Microb Cell Fact	10.1186/s12934-014-0158-4	2014
The genotype of the calcium/calmodulin-dependent protein kinase gene (CCaMK) determines bacterial community diversity in rice roots under paddy and upland field conditions.	Ikeda S, Okubo T, Takeda N, Banba M, Sasaki K, Imaizumi-Anraku H, Fujihara S, Ohwaki Y, Ohshima K, Fukuta Y, Eda S, Mitsui H, Hattori M, Sato T, Shinano T, Minamisawa K.	Appl Environ Microbiol	10.1128/aem.00315-11	2011
Biosynthesis of caffeic acid in Escherichia coli using its endogenous hydroxylase complex.	Lin Y, Yan Y.	Microb Cell Fact	10.1186/1475-2859-11-42	2012
Fermentation products of solvent tolerant marine bacterium Moraxella spp. MB1 and its biotechnological applications in salicylic acid bioconversion.	Wahidullah S, Naik DN, Devi P.	PLoS One	10.1371/journal.pone.0083647	2013
Production of cinnamic and p-hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli.	Vargas-Tah A, Martinez LM, Hernandez-Chavez G, Rocha M, Martinez A, Bolivar F, Gosset G.	Microb Cell Fact	10.1186/s12934-014-0185-1	2015
When plants produce not enough or at all: metabolic engineering of flavonoids in microbial hosts.	Trantas EA, Koffas MA, Xu P, Ververidis F.	Front Plant Sci	10.3389/fpls.2015.00007	2015
The Response of a 16S Ribosomal RNA Gene Fragment Amplified Community to Lead, Zinc, and Copper Pollution in a Shanghai Field Trial.	Kou S, Vincent G, Gonzalez E, Pitre FE, Labrecque M, Brereton NJB.	Front Microbiol	10.3389/fmicb.2018.00366	2018
Site-specific recombination strategies for engineering actinomycete genomes.	Herrmann S, Siegl T, Luzhetska M, Petzke L, Jilg C, Welle E, Erb A, Leadlay PF, Bechthold A, Luzhetskyy A.	Appl Environ Microbiol	10.1128/aem.06054-11	2012
Mechanism of 4-nitrophenol oxidation in Rhodococcus sp. Strain PN1: characterization of the two-component 4-nitrophenol hydroxylase and regulation of its expression.	Takeo M, Murakami M, Niihara S, Yamamoto K, Nishimura M, Kato D, Negoro S.	J Bacteriol	10.1128/jb.00742-08	2008
Structural determinants and modulation of substrate specificity in phenylalanine-tyrosine ammonia-lyases.	Louie GV, Bowman ME, Moffitt MC, Baiga TJ, Moore BS, Noel JP.	Chem Biol	10.1016/j.chembiol.2006.11.011	2006
Activation of a Cryptic Manumycin-Type Biosynthetic Gene Cluster of Saccharothrix espanaensis DSM44229 by Series of Genetic Manipulations.	Gorniakova D, Petricek M, Kahoun D, Grabic R, Zelenka T, Chronakova A, Petrickova K	Microorganisms	10.3390/microorganisms9030559	2021
Complete genome sequence of Saccharothrix espanaensis DSM 44229(T) and comparison to the other completely sequenced Pseudonocardiaceae.	Strobel T, Al-Dilaimi A, Blom J, Gessner A, Kalinowski J, Luzhetska M, Puhler A, Szczepanowski R, Bechthold A, Ruckert C	BMC Genomics	10.1186/1471-2164-13-465	2012
Saccharothrix tamanrassetensis sp. nov., an actinomycete isolated from Saharan soil.	Boubetra D, Zitouni A, Bouras N, Schumann P, Sproer C, Klenk HP, Sabaou N.	Int J Syst Evol Microbiol	10.1099/ijs.0.000104	2015
Reclassification of 'Dactylosporangium variesporum' as Saccharothrix variisporea corrig. (ex Tomita et al. 1977) sp. nov., nom. rev.	Kim BY, Brown R, Labeda DP, Goodfellow M.	Int J Syst Evol Microbiol	10.1099/ijs.0.021857-0	2011
Saccharothrix ghardaiensis sp. nov., an actinobacterium isolated from Saharan soil.	Bouznada K, Bouras N, Mokrane S, Chaabane Chaouch F, Zitouni A, Potter G, Sproer C, Klenk HP, Sabaou N	Antonie Van Leeuwenhoek	10.1007/s10482-016-0812-4	2016
Saccharothrix stipae sp. nov., an actinomycete isolated from the rhizosphere of Stipa grandis.	Bing Lin Y, Qing Guo Y, Hui Di X, Chun Fan M, Hong Dong D, Hong Wei G	Int J Syst Evol Microbiol	10.1099/ijsem.0.000827	2015
Saccharothrix yanglingensis sp. nov., an antagonistic endophytic actinomycete isolated from cucumber plant.	Yan X, Huang LL, Tu X, Gao XN, Kang ZS	Antonie Van Leeuwenhoek	10.1007/s10482-011-9631-9	2011
Saccharothrix obliqua sp. nov., isolated from soil of Sichang Island, Thailand.	Azad MAK, Chunhacha P, Suriyachadkun C, Sripreechasak P, Tanasupawat S, Phongsopitanun W	Int J Syst Evol Microbiol	10.1099/ijsem.0.005479	2022