Saccharothrix mutabilis subsp. capreolus (DSM 40225, ATCC 23892, CBS 678.68)

Name: Saccharothrix mutabilis subsp. capreolus (DSM 40225, ATCC 23892, CBS 678.68)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13481

Type strain

Strain Designation M48-E 2655

Culture col. no. DSM 40225 ATCC 23892 CBS 678.68 IFO 12847 ISP 5225 16 more

NCBI tax ID(s) 66854

Special Collections DSMZ JCM KCTC JMRC

History DSMZ <- E.B. Shirling, ISP (Streptomyces capreolus) <- D.H. Lively, M48-E 2655 JCM <-- KCC S-0248 <-- NRRL 2773 <-- Eli Lilly and Co.; M48-E2655.

Links

Saccharothrix mutabilis subsp. capreolus M48-E 2655 is a bacterium that produces antibiotic compounds.

antibiotic compound production genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 50444

ATCC 23892,DSM 40225,IFO 12847,NRRL 2773,JCM 4248,CCRC 13692,JCM 4630,NCIMB 9611,NRRL B-2773,VKM Ac-1848,CBS 678.68,AS 4.1351,KCTC 9395,NBRC 12847,BCRC 13692,CGMCC 4.1351,NCIMB 9801

@ref 20215

Last LPSN update 2026-05-29 08:28:03

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Saccharothrix

Species Saccharothrix mutabilis subsp. capreolus

Full scientific name Saccharothrix mutabilis subsp. capreolus (ex Higgens 1963) Grund and Kroppenstedt 1990

Synonyms (2)

"Streptomyces capreolus"
"Nocardia capreola"

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125439		98.328
125439		99.98	positive

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
9386	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
9386	positive	growth	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.911

Spore formation

@ref	Spore formation	Confidence
125439		99.793

Compound production

@ref	Compound
9386	capreomycin I
9386	capreomycin II
9386	capreomycin I, II
20216	capreomycin I
20216	capreomycin II

Observation

67770

Observationquinones: MK-8(H₂), MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
67770	3371 ChEBI	capreomycin
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
9386	-	+	+/-	-	-	+	-	-	-	+/-	+	-	-	+	-	+	-	+	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
9386	-	-	-	-	-	+	-	+	+	+	+	+	+	+	+	-	+	+	-	-	not determinedn.d.

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9386	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	IMG accession	NCBI tax ID	Score	INSDC accession
66792	Saccharothrix mutabilis capreolus DSM 40225 Capreomycin biosynthetic gene cluster	complete	2563366683	66854	89.1
124043	ASM4243104v1 assembly for Saccharothrix mutabilis subsp. capreolus JCM 4248	scaffold		66854	68.85	GCA_042431045

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Saccharothrix mutabilis subsp. capreolus partial 16S ribosomal RNA gene, strain DSM 40225	X76965	1458	66854
20218	Saccharothrix mutabilis subsp. capreolus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4248	D44038	120	66854
124043	Saccharothrix mutabilis subsp. capreolus strain JCM 4248 16S ribosomal RNA gene, partial sequence.	MT760499	1374	66854

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM4243104v1 assembly for Saccharothrix mutabilis subsp. capreolus JCM 4248
NCBI: GCA_042431045

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.91	no
125439	motility	BacteriaNetⓘ	no	98.33	no
125439	gram_stain	BacteriaNetⓘ	positive	99.98	no
125439	spore_formation	BacteriaNetⓘ	yes	99.79	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Discovery of a New Antibiotic Demethoxytetronasin Using a Dual-Sided Agar Plate Assay (DAPA).	Lee JH, Ma R, Nguyen L, Khan S, Qader M, Mpofu E, Shetye G, Krull NK, Augustinovic M, Omarsdottir S, Cho S, Franzblau SG, Murphy BT.	ACS Infect Dis	10.1021/acsinfecdis.3c00171	2023
	Crystal structure of the alpha-ketoglutarate-dependent non-heme iron oxygenase CmnC in capreomycin biosynthesis and its engineering to catalyze hydroxylation of the substrate enantiomer.	Hsiao YH, Huang SJ, Lin EC, Hsiao PY, Toh SI, Chen IH, Xu Z, Lin YP, Liu HJ, Chang CY.	Front Chem	10.3389/fchem.2022.1001311	2022
	Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus.	Saugar I, Molloy B, Sanz E, Blanca Sanchez M, Fernandez-Lobato M, Jimenez A.	J Antibiot (Tokyo)	10.1038/ja.2016.123	2017
	Discovery and characterization of genes conferring natural resistance to the antituberculosis antibiotic capreomycin.	Toh SI, Elaine Keisha J, Wang YL, Pan YC, Jhu YH, Hsiao PY, Liao WT, Chen PY, Ko TM, Chang CY.	Commun Biol	10.1038/s42003-023-05681-6	2023
	Analysis of two capreomycin-resistance determinants from Streptomyces capreolus and characterization of the action of their products.	Thiara AS, Cundliffe E.	Gene	10.1016/0378-1119(95)00702-4	1995
Pathogenicity	Identification of the biosynthetic gene cluster and an additional gene for resistance to the antituberculosis drug capreomycin.	Felnagle EA, Rondon MR, Berti AD, Crosby HA, Thomas MG	Appl Environ Microbiol	10.1128/AEM.00485-07	2007

References

#9386	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40225
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

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Saccharothrix mutabilis subsp. capreolus (DSM 40225, ATCC 23892, CBS 678.68)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042431045

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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