Halobacillus litoralis (DSM 10405, SL-4, CIP 104798)

Name: Halobacillus litoralis (DSM 10405, SL-4, CIP 104798)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1343

Type strain

Strain Designation SL-4

Culture col. no. DSM 10405 SL-4 CIP 104798

NCBI tax ID(s) 45668

Special Collections DSMZ Literature strains CIP

History <- S. Spring, Technical University Munich, strain SL-4 CIP <- 1996, S. Spring, Techn. Univ., Munchen, Germany: strain SL-4

Links

Halobacillus litoralis SL-4 is an obligate aerobe, chemoorganotroph, spore-forming bacterium that has a orange pigmentation and was isolated from hypersaline sediment.

pigmented rod-shaped motile Gram-positive spore-forming chemoorganotroph obligate aerobe Bacteria 16S sequence genome sequence

Name and taxonomic classification

SI-ID 92579

LMG 17438,DSM 10405,ATCC 700076,CIP 104798,CCM 4592,KCTC 3687

@ref 20215

Last LPSN update 2026-02-09 11:18:05

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Halobacillus

Species Halobacillus litoralis

Full scientific name Halobacillus litoralis Spring et al. 1996

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
22983	positive	2.0-4.5 µm	0.7-1.1 µm	rod-shaped		lophotrichous
120630	positive			rod-shaped

Colony morphology

Pigmentation

@ref	Production	Color
22983		orange

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3932	HALOBACILLUS MEDIUM (DSMZ Medium 755)	Medium recipe at MediaDive	Name: HALOBACILLUS MEDIUM (DSMZ Medium 755) Composition: NaCl 100.0 g/l Peptone 5.0 g/l MgSO4 x 7 H2O 5.0 g/l Yeast extract 3.0 g/l Distilled water
39099	MEDIUM 130 - for Halobacillus trueperi and Halobacillus litoralis		Distilled water make up to (1000.000 ml);Sodium chloride (30.000 g);Agar(15.000 g);Yeast extract (2.500 g);Peptone (2.500 g)
120630	CIP Medium 130	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3932	positive	growth	30
22983	positive	growth	10.0-43.0
22983	positive	optimum	35.0
39099	positive	growth	30
120630	positive	growth	10-37
120630	negative	growth	45
120630	negative	growth	55

Culture pH

@ref	Ability	Type	PH	PH range
22983	positive	optimum	7.5
22983	positive	growth	6.0-9.5	alkaliphile
120630		growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
22983	obligate aerobe
120630	obligate aerobe

Nutrition type

22983

Typechemoorganotroph

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
22983	ellipsoidal or spherical and 0.7 -1.5 µm in diameter;central to subterminal position	endospore
125438				96.75

Halophily

@ref	Salt	Growth	Tested relation	Concentration	Halophily level
22983	NaCl	positive	growth	0.5-25.0 %(w/v)
22983	NaCl		optimum	10.0 %
22983					moderately halophilic
120630	NaCl	positive	growth	2-10 %
120630	NaCl		growth	0 %

Murein

@ref	Murein short key	Type
3932	A21.04	A4ß L-Orn-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
22983		casein	-	hydrolysis
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
120630	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
22983	15824 ChEBI	D-fructose	+	builds acid from
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
22983	12936 ChEBI	D-galactose	-	builds acid from
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
22983	17634 ChEBI	D-glucose	+	builds acid from
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
22983	16899 ChEBI	D-mannitol	+	builds acid from
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
22983	16551 ChEBI	D-trehalose	+	builds acid from
22983	65327 ChEBI	D-xylose	+	builds acid from
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
22983	4853 ChEBI	esculin	-	hydrolysis
120630	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
22983	5291 ChEBI	gelatin	+	hydrolysis
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
120630	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
22983	17306 ChEBI	maltose	+	builds acid from
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
22983	17632 ChEBI	nitrate	-	reduction
120630	17632 ChEBI	nitrate	-	reduction
120630	17632 ChEBI	nitrate	-	respiration
120630	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
22983	27941 ChEBI	pullulan	-	hydrolysis
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
22983	28017 ChEBI	starch	-	hydrolysis
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
22983	17992 ChEBI	sucrose	+	builds acid from
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
22983	53426 ChEBI	tween 80	-	hydrolysis
22983	18186 ChEBI	tyrosine	-	hydrolysis
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
22983	15688 ChEBI	acetoin
120630	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
22983	15688 ChEBI	acetoin	-
120630	15688 ChEBI	acetoin	-
120630	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
120630	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120630	amylase	-
22983	arginine dihydrolase	-	3.5.3.6
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120630	caseinase	-	3.4.21.50
120630	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
22983	DNase	+
120630	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120630	gamma-glutamyltransferase	-	2.3.2.2
120630	gelatinase	+/-
22983	lecithinase	-
120630	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
120630	lipase	-
68382	lipase (C 14)	-		from API zym
120630	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
120630	ornithine decarboxylase	-	4.1.1.17
120630	oxidase	+
22983	phenylalanine deaminase	-	4.3.1.5
22983	phosphatase	-
120630	protease	-
68382	trypsin	-	3.4.21.4	from API zym
120630	tryptophan deaminase	-
120630	tween esterase	-
22983	urease	-	3.5.1.5
120630	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120630	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
120630	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120630	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Sediment
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
3932	hypersaline sediment	Utah, Great Salt Lake	USA	USA	North America
120630	Environment, Surface sediment of Great Salt Lake	Utah	United States of America	USA	North America	1993

Taxonmaps

69479

Global distribution of 16S sequence X94558 (>99% sequence identity) for Halobacillus from Microbeatlas

Aquatic Samples	2677
Soil Samples	2306
Animal Samples	590
Plant Samples	407
Total Samples	5980

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3932	1	Risk group (German classification) According to TRBA 466
120630	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM985644v1 assembly for Halobacillus litoralis SL-4	contig	GCA_009856445	45668.7	8002335811	45668	65.84

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	H.litoralis 16S rRNA gene	X94558	1559		45668

GC content

22983

GC-content (mol%)42

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Halobacillus litoralis SL-4
NCBI: GCA_009856445.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	77.74	yes
125438	anaerobic	anaerobicⓘ	no	91.77	yes
125438	aerobic	aerobicⓘ	yes	84.08	yes
125438	spore-forming	spore-formingⓘ	yes	96.75	yes
125438	thermophilic	thermophileⓘ	no	96.24	yes
125438	flagellated	motile2+ⓘ	yes	87.18	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Biology of moderately halophilic aerobic bacteria.	Ventosa A, Nieto JJ, Oren A.	Microbiol Mol Biol Rev	10.1128/mmbr.62.2.504-544.1998	1998
Phylogeny	Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.	Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang J	Int J Syst Evol Microbiol	10.1099/ijs.0.000198	2015

References

#3932	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10405
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#22983	S. SPRING,W. LUDWIG,M. C. MARQUEZ,A. VENTOSA,K.-H. SCHLEIFER: Halobacillus gen. nov., with Descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and Transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov.. IJSEM 46: 492 - 496 1996 ( DOI 10.1099/00207713-46-2-492 )
#39099	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120630	Collection of Institut Pasteur ; Curators of the CIP; CIP 104798
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Halobacillus litoralis (DSM 10405, SL-4, CIP 104798)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_009856445

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media