Name: Halobacillus trueperi SL-5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1342

Type strain:

Species: Halobacillus trueperi

Strain Designation: SL-5

Culture col. no.: DSM 10404, CIP 104797

Strain history: CIP <- 1996, S. Spring, Techn. Univ., Munchen, Germany: strain SL-5

NCBI tax ID(s): 156205 (species)

SI-ID 92578

LMG 17437, DSM 10404, ATCC 700077, CIP 104797, CCM 4593, KCTC 3686

Section

Halobacillus trueperi SL-5 is an obligate aerobe, chemoorganotroph, spore-forming bacterium that has a orange pigmentation and was isolated from hypersaline sediments.

Gram-positive
motile
pigmented
rod-shaped
spore-forming
chemoorganotroph
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Halobacillus*
	Species	**Halobacillus trueperi**
	Full Scientific Name (LPSN)	Halobacillus trueperi Spring et al. 1996

Information on morphological and physiological properties Morphology

[Ref.: #22983]	Gram stain	positive
[Ref.: #123595]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #22983]	Cell length	2.0-4.5 µm

[Ref.: #22983]	Cell width	0.7-1.4 µm

[Ref.: #22983]	Cell shape	rod-shaped
[Ref.: #123595]	Cell shape	rod-shaped

[Ref.: #22983]	Motility	yes
[Ref.: #123595]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %95.558

[Ref.: #22983]	Flagellum arrangement	lophotrichous

[Ref.: #22983]	Pigment production	yes
[Ref.: #22983]	Pigmentation color	orange

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3931]

Culture medium

HALOBACILLUS MEDIUM (DSMZ Medium 755)

[Ref.: #3931]

Culture medium growth

[Ref.: #3931]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3931]

Culture medium composition

expand / minimize

[Ref.: #39098]

Culture medium

MEDIUM 130 - for Halobacillus trueperi and Halobacillus litoralis

[Ref.: #39098]

Culture medium growth

[Ref.: #39098]

Culture medium composition

expand / minimize

[Ref.: #123595]

Culture medium

CIP Medium 130

[Ref.: #123595]

Culture medium growth

[Ref.: #123595]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3931]		growth	30 °C
[Ref.: #22983]		growth	10.0-44.0 °C
[Ref.: #22983]		optimum	35.0 °C
[Ref.: #39098]		growth	30 °C
[Ref.: #123595]		growth	10-37 °C
[Ref.: #123595]	no	growth	45 °C
[Ref.: #123595]	no	growth	55 °C

[Ref.: #3931]	Temperature range	mesophilic
[Ref.: #22983]	Temperature range	mesophilic
[Ref.: #39098]	Temperature range	mesophilic
[Ref.: #123595]	Temperature range	thermophilic

	Kind of pH		pH
[Ref.: #22983]		optimum	7.5
[Ref.: #22983]		growth	6.0-9.5
[Ref.: #123595]	no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22983]	Oxygen tolerance	obligate aerobe
[Ref.: #123595]	Oxygen tolerance	obligate aerobe

[Ref.: #22983]

Nutrition type

chemoorganotroph

[Ref.: #22983]	Spore description	ellipsoidal or spherical and 0.7-1.5 µm in diameter;central to subterminal position
[Ref.: #22983]	Type of spore	endospore
[Ref.: #22983]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #22983]

Halophily / tolerance level

moderately halophilic

	Salt	Tested relation		Salt conc.
[Ref.: #22983]	NaCl		growth	0.5-30.0	%(w/v)
[Ref.: #22983]	NaCl		optimum	10.0	%
[Ref.: #123595]	NaCl		growth	2-10	%
[Ref.: #123595]	NaCl	no	growth	0	%

[Ref.: #3931]	Murein short key	A21.04
[Ref.: #3931]	Murein types	A4ß L-Orn-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22983]	casein	-	hydrolysis
[Ref.: #123595]	citrate ChEBI	-	carbon source
[Ref.: #22983]	D-fructose ChEBI	+	builds acid from
[Ref.: #22983]	D-galactose ChEBI	+	builds acid from
[Ref.: #22983]	D-glucose ChEBI	+	builds acid from
[Ref.: #22983]	D-mannitol ChEBI	-	builds acid from
[Ref.: #22983]	D-trehalose ChEBI	+	builds acid from
[Ref.: #22983]	D-xylose ChEBI	-	builds acid from
[Ref.: #22983]	esculin ChEBI	-	hydrolysis
[Ref.: #123595]	esculin ChEBI	-	hydrolysis
[Ref.: #22983]	gelatin ChEBI	+	hydrolysis
[Ref.: #123595]	hippurate ChEBI	-	hydrolysis
[Ref.: #22983]	maltose ChEBI	+	builds acid from
[Ref.: #22983]	nitrate ChEBI	-	reduction
[Ref.: #123595]	nitrate ChEBI	-	reduction
[Ref.: #123595]	nitrate ChEBI	-	respiration
[Ref.: #123595]	nitrite ChEBI	-	reduction
[Ref.: #22983]	pullulan ChEBI	+	hydrolysis
[Ref.: #22983]	starch ChEBI	-	hydrolysis
[Ref.: #22983]	sucrose ChEBI	+	builds acid from
[Ref.: #22983]	tween 80 ChEBI	-	hydrolysis
[Ref.: #22983]	tyrosine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #22983]		acetoin ChEBI	no
[Ref.: #123595]		indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #22983]	acetoin ChEBI	-
[Ref.: #123595]	acetoin ChEBI	-
[Ref.: #123595]	glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #123595]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123595]	amylase	-
[Ref.: #22983]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123595]	caseinase	-	3.4.21.50
[Ref.: #123595]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #22983]	DNase	+
[Ref.: #123595]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123595]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #123595]	gelatinase	+/-
[Ref.: #22983]	lecithinase	-
[Ref.: #123595]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #123595]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123595]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #123595]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123595]	oxidase	+
[Ref.: #22983]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #22983]	phosphatase	-
[Ref.: #123595]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123595]	tryptophan deaminase	-
[Ref.: #123595]	tween esterase	-
[Ref.: #22983]	urease	-	3.5.1.5
[Ref.: #123595]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123595]	8204	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3931]	Sample type/isolated from	hypersaline sediments
[Ref.: #3931]	Geographic location (country and/or sea, region)	Utah, Great Salt Lake
[Ref.: #3931]	Country	USA
[Ref.: #3931]	Country ISO 3 Code	USA
[Ref.: #3931]	Continent	North America

[Ref.: #123595]	Sample type/isolated from	Environment, Surface sediment of Great Salt Lake
[Ref.: #123595]	Geographic location (country and/or sea, region)	Utah
[Ref.: #123595]	Country	United States of America
[Ref.: #123595]	Country ISO 3 Code	USA
[Ref.: #123595]	Continent	North America
[Ref.: #123595]	Isolation date	1993
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Sediment
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ310149 (>99% sequence identity) for Halobacillus from Microbeatlas

Aquatic Samples	2677
Soil Samples	2306
Animal Samples	590
Plant Samples	407
Total Samples	5980

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3931]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123595]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Halobacillus trueperi gene for 16S ribosomal RNA, 23S ribosomal RNA, tRNA-Ileu, tRNA-Ala, partial and complete sequence, clone: 850	AB243768	691	ENA	156205 tax ID	*
[Ref.: #20218]	Halobacillus trueperi gene for 16S ribosomal RNA, 23S ribosomal RNA, tRNA-Ileu, tRNA-Ala, partial and complete sequence, clone: 700	AB243769	691	ENA	156205 tax ID	*
[Ref.: #20218]	Halobacillus trueperi gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 550	AB243770	568	ENA	156205 tax ID	*
[Ref.: #20218]	Halobacillus trueperi gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 450	AB243771	450	ENA	156205 tax ID	*
[Ref.: #20218]	Halobacillus trueperi 16S rRNA gene, strain DSM 10404T	AJ310149	1536	ENA	156205 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Halobacillus trueperi strain KCTC 3686	156205.9	wgs	PATRIC	156205 tax ID	*

[Ref.: #22983]

GC-content

43 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Halobacillus trueperi strain KCTC 3686 PATRIC: 156205.8
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Halobacillus trueperi strain KCTC 3686 PATRIC: 156205.9
[Ref.: #69480]	motile	yes	90.061	no
[Ref.: #69480]	gram-positive	yes	89.633	yes
[Ref.: #69480]	anaerobic	no	99.013	yes
[Ref.: #69480]	aerobic	yes	97.464	yes
[Ref.: #69480]	halophile	yes	93.825	no
[Ref.: #69480]	spore-forming	yes	95.167	no
[Ref.: #69480]	thermophile	no	97.885	no
[Ref.: #69480]	glucose-util	yes	88.932	no
[Ref.: #69480]	flagellated	yes	88.341	yes
[Ref.: #69480]	glucose-ferment	no	89.923	no

Availability in culture collections External links

[Ref.: #3931]

Culture collection no.

DSM 10404, CIP 104797

[Ref.: #70992]

SI-ID 92578

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Taxonomic description and genome sequence of Halobacillus marinus sp. nov., a novel strain isolated from Chilika Lake, India.	Panda AN, Mishra SR, Ray L, Das S, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina V	J Microbiol	10.1007/s12275-018-7387-x	2018	*
Phylogeny	Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.	Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang J	Int J Syst Evol Microbiol	10.1099/ijs.0.000198	2015	*
Metabolism	Cloning and characterization of the genes encoding a glycine betaine ABC-type transporter in Halobacillus trueperi DSM10404T.	Lu W, Zhang B, Zhao B, Feng D, Yang L, Wang L, Yang S	Curr Microbiol	10.1007/s00284-006-0235-y	2006	*

References

#3931

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10404

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22983

S. SPRING,W. LUDWIG,M. C. MARQUEZ,A. VENTOSA,K.-H. SCHLEIFER: Halobacillus gen. nov., with Descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and Transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov.. IJSEM 46: 492 - 496 1996 ( DOI 10.1099/00207713-46-2-492 )

#39098 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70992

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92578.1 )

#123595 Collection of Institut Pasteur ; Curators of the CIP; CIP 104797
* These data were automatically processed and therefore are not curated

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Halobacillus trueperi strain KCTC 3686

Halobacillus trueperi strain KCTC 3686

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