Halobacillus trueperi (DSM 10404, SL-5, CIP 104797)

Name: Halobacillus trueperi (DSM 10404, SL-5, CIP 104797)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1342

Type strain

Strain Designation SL-5

Culture col. no. DSM 10404 SL-5 CIP 104797

NCBI tax ID(s) 156205

Special Collections DSMZ Literature strains CIP

Links

Halobacillus trueperi SL-5 is an obligate aerobe, chemoorganotroph, spore-forming prokaryote that has a orange pigmentation and was isolated from hypersaline sediments.

pigmented rod-shaped motile Gram-positive spore-forming mesophilic chemoorganotroph obligate aerobe 16S sequence genome sequence

Name and taxonomic classification

SI-ID 92578

LMG 17437,DSM 10404,ATCC 700077,CIP 104797,CCM 4593,KCTC 3686

@ref 20215

Last LPSN update 2025-12-16 09:47:05

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Halobacillus

Species Halobacillus trueperi

Full scientific name Halobacillus trueperi Spring et al. 1996

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
22983	positive	2.0-4.5 µm	0.7-1.4 µm	rod-shaped		lophotrichous
123595	positive			rod-shaped

Colony morphology

Pigmentation

@ref	Production	Color
22983		orange

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3931	HALOBACILLUS MEDIUM (DSMZ Medium 755)	Medium recipe at MediaDive	Name: HALOBACILLUS MEDIUM (DSMZ Medium 755) Composition: NaCl 100.0 g/l Peptone 5.0 g/l MgSO4 x 7 H2O 5.0 g/l Yeast extract 3.0 g/l Distilled water
39098	MEDIUM 130 - for Halobacillus trueperi and Halobacillus litoralis		Distilled water make up to (1000.000 ml);Sodium chloride (30.000 g);Agar(15.000 g);Yeast extract (2.500 g);Peptone (2.500 g)
123595	CIP Medium 130	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3931	positive	growth	30	mesophilic
22983	positive	growth	10.0-44.0
22983	positive	optimum	35.0	mesophilic
39098	positive	growth	30	mesophilic
123595	positive	growth	10-37
123595	negative	growth	45
123595	negative	growth	55

Culture pH

@ref	Ability	Type	PH	PH range
22983	positive	optimum	7.5
22983	positive	growth	6.0-9.5	alkaliphile
123595		growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
22983	obligate aerobe
123595	obligate aerobe

Nutrition type

22983

Typechemoorganotroph

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
22983	ellipsoidal or spherical and 0.7-1.5 µm in diameter;central to subterminal position	endospore
125438				96.49

Halophily

@ref	Salt	Growth	Tested relation	Concentration	Halophily level
22983	NaCl	positive	growth	0.5-30.0 %(w/v)
22983	NaCl		optimum	10.0 %
22983					moderately halophilic
123595	NaCl	positive	growth	2-10 %
123595	NaCl		growth	0 %

Murein

@ref	Murein short key	Type
3931	A21.04	A4ß L-Orn-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
22983		casein	-	hydrolysis
123595	16947 ChEBI	citrate	-	carbon source
22983	15824 ChEBI	D-fructose	+	builds acid from
22983	12936 ChEBI	D-galactose	+	builds acid from
22983	17634 ChEBI	D-glucose	+	builds acid from
22983	16899 ChEBI	D-mannitol	-	builds acid from
22983	16551 ChEBI	D-trehalose	+	builds acid from
22983	65327 ChEBI	D-xylose	-	builds acid from
22983	4853 ChEBI	esculin	-	hydrolysis
123595	4853 ChEBI	esculin	-	hydrolysis
22983	5291 ChEBI	gelatin	+	hydrolysis
123595	606565 ChEBI	hippurate	-	hydrolysis
22983	17306 ChEBI	maltose	+	builds acid from
22983	17632 ChEBI	nitrate	-	reduction
123595	17632 ChEBI	nitrate	-	reduction
123595	17632 ChEBI	nitrate	-	respiration
123595	16301 ChEBI	nitrite	-	reduction
22983	27941 ChEBI	pullulan	+	hydrolysis
22983	28017 ChEBI	starch	-	hydrolysis
22983	17992 ChEBI	sucrose	+	builds acid from
22983	53426 ChEBI	tween 80	-	hydrolysis
22983	18186 ChEBI	tyrosine	-	hydrolysis

Metabolite production

@ref	Chebi-ID	Metabolite	Production
22983	15688 ChEBI	acetoin
123595	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
22983	15688 ChEBI	acetoin	-
123595	15688 ChEBI	acetoin	-
123595	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
123595	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123595	amylase	-
22983	arginine dihydrolase	-	3.5.3.6
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123595	caseinase	-	3.4.21.50
123595	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
22983	DNase	+
123595	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123595	gamma-glutamyltransferase	-	2.3.2.2
123595	gelatinase	+/-
22983	lecithinase	-
123595	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
123595	lipase	-
68382	lipase (C 14)	-		from API zym
123595	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
123595	ornithine decarboxylase	-	4.1.1.17
123595	oxidase	+
22983	phenylalanine deaminase	-	4.3.1.5
22983	phosphatase	-
123595	protease	-
68382	trypsin	-	3.4.21.4	from API zym
123595	tryptophan deaminase	-
123595	tween esterase	-
22983	urease	-	3.5.1.5
123595	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123595	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Sediment
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
3931	hypersaline sediments	Utah, Great Salt Lake	USA	USA	North America
123595	Environment, Surface sediment of Great Salt Lake	Utah	United States of America	USA	North America	1993

Taxonmaps

69479

Global distribution of 16S sequence AJ310149 (>99% sequence identity) for Halobacillus from Microbeatlas

Aquatic Samples	2677
Soil Samples	2306
Animal Samples	590
Plant Samples	407
Total Samples	5980

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3931	1	Risk group (German classification) According to TRBA 466
123595	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM338694v1 assembly for Halobacillus trueperi KCTC 3686	contig	GCA_003386945	156205.9	2837540175	156205	62.56

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Halobacillus trueperi 16S rRNA gene, strain DSM 10404T	AJ310149	1536	156205
124043	Halobacillus trueperi strain DSM 10404 16S ribosomal RNA gene, partial sequence.	OQ626006	1380	156205
124043	Halobacillus trueperi strain DSM 10404 16S ribosomal RNA gene, partial sequence.	MW198072	601	156205
124043	Halobacillus trueperi strain DSM 10404(T) 16S ribosomal RNA gene, partial sequence.	MZ666903	736	156205
124043	Halobacillus trueperi strain DSM 10404(T) 16S ribosomal RNA gene, partial sequence.	MZ666928	806	156205

GC content

22983

GC-content (mol%)43

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Halobacillus trueperi strain KCTC 3686
PATRIC: 156205.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	80.53	yes
125438	anaerobic	anaerobicⓘ	no	96.87	yes
125438	spore-forming	spore-formingⓘ	yes	96.49	yes
125438	aerobic	aerobicⓘ	yes	85.03	no
125438	thermophilic	thermophileⓘ	no	94.76	yes
125438	flagellated	motile2+ⓘ	yes	85.73	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Optimization, partial purification, and characterization of a novel high molecular weight alkaline protease produced by Halobacillus sp. HAL1 using fish wastes as a substrate.	Fahmy NM, El-Deeb B.	J Genet Eng Biotechnol	10.1186/s43141-023-00509-6	2023
	Diversity and Biotechnological Potential of Cultivable Halophilic and Halotolerant Bacteria from the "Los Negritos" Geothermal Area.	Guevara-Luna J, Arroyo-Herrera I, Tapia-Garcia EY, Estrada-de Los Santos P, Ortega-Nava AJ, Vasquez-Murrieta MS.	Microorganisms	10.3390/microorganisms12030482	2024
	Biology of moderately halophilic aerobic bacteria.	Ventosa A, Nieto JJ, Oren A.	Microbiol Mol Biol Rev	10.1128/mmbr.62.2.504-544.1998	1998
Metabolism	Cloning and characterization of the genes encoding a glycine betaine ABC-type transporter in Halobacillus trueperi DSM10404T.	Lu W, Zhang B, Zhao B, Feng D, Yang L, Wang L, Yang S	Curr Microbiol	10.1007/s00284-006-0235-y	2006
Phylogeny	Taxonomic description and genome sequence of Halobacillus marinus sp. nov., a novel strain isolated from Chilika Lake, India.	Panda AN, Mishra SR, Ray L, Das S, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina V	J Microbiol	10.1007/s12275-018-7387-x	2018
Phylogeny	Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.	Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang J	Int J Syst Evol Microbiol	10.1099/ijs.0.000198	2015

References

#3931	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10404
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#22983	S. SPRING,W. LUDWIG,M. C. MARQUEZ,A. VENTOSA,K.-H. SCHLEIFER: Halobacillus gen. nov., with Descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and Transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov.. IJSEM 46: 492 - 496 1996 ( DOI 10.1099/00207713-46-2-492 )
#39098	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123595	Collection of Institut Pasteur ; Curators of the CIP; CIP 104797
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Halobacillus trueperi (DSM 10404, SL-5, CIP 104797)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003386945

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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