Strain identifier

BacDive ID: 1342

Type strain: Yes

Species: Halobacillus trueperi

Strain Designation: SL-5

Strain history: CIP <- 1996, S. Spring, Techn. Univ., Munchen, Germany: strain SL-5

NCBI tax ID(s): 156205 (species)

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General

@ref: 3931

BacDive-ID: 1342

DSM-Number: 10404

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, chemoorganotroph, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, pigmented

description: Halobacillus trueperi SL-5 is an obligate aerobe, chemoorganotroph, spore-forming bacterium that has a orange pigmentation and was isolated from hypersaline sediments.

NCBI tax id

  • NCBI tax id: 156205
  • Matching level: species

strain history

@refhistory
3931<- S. Spring, Technical University Munich, strain SL-5
123595CIP <- 1996, S. Spring, Techn. Univ., Munchen, Germany: strain SL-5

doi: 10.13145/bacdive1342.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Halobacillus
  • species: Halobacillus trueperi
  • full scientific name: Halobacillus trueperi Spring et al. 1996

@ref: 3931

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Halobacillus

species: Halobacillus trueperi

full scientific name: Halobacillus trueperi Spring et al. 1996

strain designation: SL-5

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
22983positive2.0-4.5 µm0.7-1.4 µmrod-shapedyeslophotrichous
69480yes95.558
69480positive100
123595positiverod-shapedno

colony morphology

  • @ref: 123595

pigmentation

  • @ref: 22983
  • production: yes
  • color: orange

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3931HALOBACILLUS MEDIUM (DSMZ Medium 755)yeshttps://mediadive.dsmz.de/medium/755Name: HALOBACILLUS MEDIUM (DSMZ Medium 755) Composition: NaCl 100.0 g/l Peptone 5.0 g/l MgSO4 x 7 H2O 5.0 g/l Yeast extract 3.0 g/l Distilled water
39098MEDIUM 130 - for Halobacillus trueperi and Halobacillus litoralisyesDistilled water make up to (1000.000 ml);Sodium chloride (30.000 g);Agar(15.000 g);Yeast extract (2.500 g);Peptone (2.500 g)
123595CIP Medium 130yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=130

culture temp

@refgrowthtypetemperaturerange
3931positivegrowth30mesophilic
22983positivegrowth10.0-44.0
22983positiveoptimum35.0mesophilic
39098positivegrowth30mesophilic
123595positivegrowth10-37
123595nogrowth45thermophilic
123595nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
22983positivegrowth6.0-9.5alkaliphile
22983positiveoptimum7.5
123595nogrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22983obligate aerobe
123595obligate aerobe

nutrition type

  • @ref: 22983
  • type: chemoorganotroph

spore formation

@refspore descriptiontype of sporespore formationconfidence
22983ellipsoidal or spherical and 0.7-1.5 µm in diameter;central to subterminal positionendosporeyes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentrationhalophily level
22983NaClpositivegrowth0.5-30.0 %(w/v)
22983NaCloptimum10.0 %
22983moderately halophilic
123595NaClpositivegrowth2-10 %
123595NaClnogrowth0 %

murein

  • @ref: 3931
  • murein short key: A21.04
  • type: A4ß L-Orn-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2298316899D-mannitol-builds acid from
2298365327D-xylose-builds acid from
22983casein-hydrolysis
229834853esculin-hydrolysis
2298328017starch-hydrolysis
2298353426tween 80-hydrolysis
2298318186tyrosine-hydrolysis
2298317632nitrate-reduction
2298315824D-fructose+builds acid from
2298312936D-galactose+builds acid from
2298317634D-glucose+builds acid from
2298316551D-trehalose+builds acid from
2298317306maltose+builds acid from
2298317992sucrose+builds acid from
229835291gelatin+hydrolysis
2298327941pullulan+hydrolysis
12359516947citrate-carbon source
1235954853esculin-hydrolysis
123595606565hippurate-hydrolysis
12359517632nitrate-reduction
12359516301nitrite-reduction
12359517632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
2298315688acetoinno
12359535581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2298315688acetoin-
12359515688acetoin-
12359517234glucose-

enzymes

@refvalueactivityec
22983arginine dihydrolase-3.5.3.6
22983DNase+
22983lecithinase-
22983phenylalanine deaminase-4.3.1.5
22983phosphatase-
22983urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
123595oxidase+
123595alcohol dehydrogenase-1.1.1.1
123595gelatinase+/-
123595amylase-
123595DNase+
123595caseinase-3.4.21.50
123595catalase+1.11.1.6
123595tween esterase-
123595gamma-glutamyltransferase-2.3.2.2
123595lecithinase-
123595lipase-
123595lysine decarboxylase-4.1.1.18
123595ornithine decarboxylase-4.1.1.17
123595protease-
123595tryptophan deaminase-
123595urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123595--++---------+------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
3931hypersaline sedimentsUtah, Great Salt LakeUSAUSANorth America
123595Environment, Surface sediment of Great Salt LakeUtahUnited States of AmericaUSANorth America1993

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Sediment
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_774.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_469;97_536;98_617;99_774&stattab=map
  • Last taxonomy: Halobacillus
  • 16S sequence: AJ310149
  • Sequence Identity:
  • Total samples: 5980
  • soil counts: 2306
  • aquatic counts: 2677
  • animal counts: 590
  • plant counts: 407

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
39311Risk group (German classification)
1235951Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Halobacillus trueperi gene for 16S ribosomal RNA, 23S ribosomal RNA, tRNA-Ileu, tRNA-Ala, partial and complete sequence, clone: 850AB243768691ena156205
20218Halobacillus trueperi gene for 16S ribosomal RNA, 23S ribosomal RNA, tRNA-Ileu, tRNA-Ala, partial and complete sequence, clone: 700AB243769691ena156205
20218Halobacillus trueperi gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 550AB243770568ena156205
20218Halobacillus trueperi gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 450AB243771450ena156205
20218Halobacillus trueperi 16S rRNA gene, strain DSM 10404TAJ3101491536ena156205

Genome sequences

  • @ref: 66792
  • description: Halobacillus trueperi strain KCTC 3686
  • accession: 156205.9
  • assembly level: wgs
  • database: patric
  • NCBI tax ID: 156205

GC content

  • @ref: 22983
  • GC-content: 43

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes90.061no
gram-positiveyes89.633yes
anaerobicno99.013yes
aerobicyes97.464yes
halophileyes93.825no
spore-formingyes95.167no
thermophileno97.885no
glucose-utilyes88.932no
flagellatedyes88.341yes
glucose-fermentno89.923no

External links

@ref: 3931

culture collection no.: DSM 10404, CIP 104797

straininfo link

  • @ref: 70992
  • straininfo: 92578

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism17171468Cloning and characterization of the genes encoding a glycine betaine ABC-type transporter in Halobacillus trueperi DSM10404T.Lu W, Zhang B, Zhao B, Feng D, Yang L, Wang L, Yang SCurr Microbiol10.1007/s00284-006-0235-y2006ATP-Binding Cassette Transporters/chemistry/*genetics/*metabolism, Amino Acid Sequence, Bacillaceae/genetics/*metabolism, Bacterial Proteins/chemistry/genetics/metabolism, Betaine/*metabolism, *Cloning, Molecular, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNAEnzymology
Phylogeny25795064Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang JInt J Syst Evol Microbiol10.1099/ijs.0.0001982015Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Halobacillus/*classification/genetics/isolation & purification, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Soil/chemistry, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny29611137Taxonomic description and genome sequence of Halobacillus marinus sp. nov., a novel strain isolated from Chilika Lake, India.Panda AN, Mishra SR, Ray L, Das S, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina VJ Microbiol10.1007/s12275-018-7387-x2018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, *Genome, Bacterial, Genotype, Halobacillus/chemistry/*classification/*genetics/isolation & purification, India, Lakes/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3931Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10404)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10404
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22983S. SPRING,W. LUDWIG,M. C. MARQUEZ,A. VENTOSA,K.-H. SCHLEIFER10.1099/00207713-46-2-492Halobacillus gen. nov., with Descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and Transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov.IJSEM 46: 492-496 1996
39098Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16745
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70992Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92578.1StrainInfo: A central database for resolving microbial strain identifiers
123595Curators of the CIPCollection of Institut Pasteur (CIP 104797)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104797