Strain identifier

BacDive ID: 1343

Type strain: Yes

Species: Halobacillus litoralis

Strain Designation: SL-4

Strain history: CIP <- 1996, S. Spring, Techn. Univ., Munchen, Germany: strain SL-4

NCBI tax ID(s): 45668 (species)

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General

@ref: 3932

BacDive-ID: 1343

DSM-Number: 10405

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, chemoorganotroph, spore-forming, Gram-positive, motile, rod-shaped, pigmented

description: Halobacillus litoralis SL-4 is an obligate aerobe, chemoorganotroph, spore-forming bacterium that has a orange pigmentation and was isolated from hypersaline sediment.

NCBI tax id

  • NCBI tax id: 45668
  • Matching level: species

strain history

@refhistory
3932<- S. Spring, Technical University Munich, strain SL-4
120630CIP <- 1996, S. Spring, Techn. Univ., Munchen, Germany: strain SL-4

doi: 10.13145/bacdive1343.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Halobacillus
  • species: Halobacillus litoralis
  • full scientific name: Halobacillus litoralis Spring et al. 1996

@ref: 3932

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Halobacillus

species: Halobacillus litoralis

full scientific name: Halobacillus litoralis Spring et al. 1996

strain designation: SL-4

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
22983positive2.0-4.5 µm0.7 -1.1 µmrod-shapedyeslophotrichous
120630positiverod-shapedno

colony morphology

  • @ref: 120630

pigmentation

  • @ref: 22983
  • production: yes
  • color: orange

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3932HALOBACILLUS MEDIUM (DSMZ Medium 755)yeshttps://mediadive.dsmz.de/medium/755Name: HALOBACILLUS MEDIUM (DSMZ Medium 755) Composition: NaCl 100.0 g/l Peptone 5.0 g/l MgSO4 x 7 H2O 5.0 g/l Yeast extract 3.0 g/l Distilled water
39099MEDIUM 130 - for Halobacillus trueperi and Halobacillus litoralisyesDistilled water make up to (1000.000 ml);Sodium chloride (30.000 g);Agar(15.000 g);Yeast extract (2.500 g);Peptone (2.500 g)
120630CIP Medium 130yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=130

culture temp

@refgrowthtypetemperature
3932positivegrowth30
22983positivegrowth10.0-43.0
22983positiveoptimum35.0
39099positivegrowth30
120630positivegrowth10-37
120630nogrowth45
120630nogrowth55

culture pH

@refabilitytypepHPH range
22983positivegrowth6.0-9.5alkaliphile
22983positiveoptimum7.5
120630nogrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22983obligate aerobe
120630obligate aerobe

nutrition type

  • @ref: 22983
  • type: chemoorganotroph

spore formation

@refspore descriptiontype of sporespore formationconfidence
22983ellipsoidal or spherical and 0.7 -1.5 µm in diameter;central to subterminal positionendosporeyes
69480yes96.75

halophily

@refsaltgrowthtested relationconcentrationhalophily level
22983NaClpositivegrowth0.5-25.0 %(w/v)
22983NaCloptimum10.0 %
22983moderately halophilic
120630NaClpositivegrowth2-10 %
120630NaClnogrowth0 %

murein

  • @ref: 3932
  • murein short key: A21.04
  • type: A4ß L-Orn-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2298312936D-galactose-builds acid from
22983casein-hydrolysis
229834853esculin-hydrolysis
2298327941pullulan-hydrolysis
2298328017starch-hydrolysis
2298353426tween 80-hydrolysis
2298318186tyrosine-hydrolysis
2298317632nitrate-reduction
2298315824D-fructose+builds acid from
2298317634D-glucose+builds acid from
2298316899D-mannitol+builds acid from
2298316551D-trehalose+builds acid from
2298365327D-xylose+builds acid from
2298317306maltose+builds acid from
2298317992sucrose+builds acid from
229835291gelatin+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12063016947citrate-carbon source
1206304853esculin-hydrolysis
120630606565hippurate-hydrolysis
12063017632nitrate-reduction
12063016301nitrite-reduction
12063017632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
2298315688acetoinno
12063035581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2298315688acetoin-
12063015688acetoin-
12063017234glucose-

enzymes

@refvalueactivityec
22983arginine dihydrolase-3.5.3.6
22983DNase+
22983lecithinase-
22983phenylalanine deaminase-4.3.1.5
22983phosphatase-
22983urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
120630oxidase+
120630alcohol dehydrogenase-1.1.1.1
120630gelatinase+/-
120630amylase-
120630DNase+
120630caseinase-3.4.21.50
120630catalase+1.11.1.6
120630tween esterase-
120630gamma-glutamyltransferase-2.3.2.2
120630lecithinase-
120630lipase-
120630lysine decarboxylase-4.1.1.18
120630ornithine decarboxylase-4.1.1.17
120630protease-
120630tryptophan deaminase-
120630urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120630--++-----------+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120630-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120630---------------------------------------------------------------------------+-----------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
3932hypersaline sedimentUtah, Great Salt LakeUSAUSANorth America
120630Environment, Surface sediment of Great Salt LakeUtahUnited States of AmericaUSANorth America1993

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Sediment
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_774.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_469;97_536;98_617;99_774&stattab=map
  • Last taxonomy: Halobacillus
  • 16S sequence: X94558
  • Sequence Identity:
  • Total samples: 5980
  • soil counts: 2306
  • aquatic counts: 2677
  • animal counts: 590
  • plant counts: 407

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
39321Risk group (German classification)
1206301Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Halobacillus litoralis gene for 16S ribosomal RNA, 23S ribosomal RNA, tRNA-Ileu, tRNA-Ala, partial and complete sequence, clone: 850AB243772644nuccore45668
20218Halobacillus litoralis gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 700AB243773448nuccore45668
20218Halobacillus litoralis gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 550AB243774566nuccore45668
20218Halobacillus litoralis gene for 16S ribosomal RNA, ITS1, 23S ribosomal RNA, partial and complete sequence, clone: 450AB243775448nuccore45668
20218H.litoralis 16S rRNA geneX945581559nuccore45668

Genome sequences

  • @ref: 66792
  • description: Halobacillus litoralis SL-4
  • accession: GCA_009856445
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 45668

GC content

  • @ref: 22983
  • GC-content: 42

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes77.737yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no91.772yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes84.077yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes96.75yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno96.242yes
69480flagellatedmotile2+Ability to perform flagellated movementyes87.184yes

External links

@ref: 3932

culture collection no.: DSM 10405, CIP 104798

straininfo link

  • @ref: 70993
  • straininfo: 92579

literature

  • topic: Phylogeny
  • Pubmed-ID: 25795064
  • title: Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.
  • authors: Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.000198
  • year: 2015
  • mesh: Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Halobacillus/*classification/genetics/isolation & purification, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Soil/chemistry, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3932Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10405)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10405
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22983S. SPRING,W. LUDWIG,M. C. MARQUEZ,A. VENTOSA,K.-H. SCHLEIFER10.1099/00207713-46-2-492Halobacillus gen. nov., with Descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and Transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov.IJSEM 46: 492-496 1996
39099Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16746
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
70993Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92579.1StrainInfo: A central database for resolving microbial strain identifiers
120630Curators of the CIPCollection of Institut Pasteur (CIP 104798)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104798