Name: Lysinibacillus alkalisoli Y2A20
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133312

Type strain:

Species: Lysinibacillus alkalisoli

Strain Designation: Y2A20

Culture col. no.: CGMCC 1.15760, KCTC 33825

NCBI tax ID(s): 1911548 (species)

Section

Lysinibacillus alkalisoli Y2A20 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from saline–alkaline soil.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Caryophanaceae*
	Genus	*Lysinibacillus*
	Species	**Lysinibacillus alkalisoli**
	Full Scientific Name (LPSN)	Lysinibacillus alkalisoli Sun et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25154]	Gram stain	positive

[Ref.: #25154]	Cell length	1.3-2.6 µm

[Ref.: #25154]	Cell width	0.6-1.0 µm

[Ref.: #25154]	Cell shape	rod-shaped

[Ref.: #25154]	Motility	yes

[Ref.: #25154]	Flagellum arrangement	peritrichous

[Ref.: #25154]	Colony size	2.0-3.0 mm
[Ref.: #25154]	Colony color	ivory
[Ref.: #25154]	Colony shape	circular
[Ref.: #25154]	Incubation period	2 days
[Ref.: #25154]	Cultivation medium used	LB

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25154]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #25154]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #25154]			optimum	30 °C
[Ref.: #25154]			growth	10-45 °C

[Ref.: #25154]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #25154]			optimum	8.0-9.0
[Ref.: #25154]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25154]

Oxygen tolerance

aerobe

[Ref.: #25154]	Spore description	Ellipsoidal in terminal position in swollen sporangium
[Ref.: #25154]	Type of spore	endospore
[Ref.: #25154]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25154]		NaCl		optimum	1.0-2.0	%
[Ref.: #25154]		NaCl		growth	0.0-0.8	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25154]	acetate ChEBI	-	assimilation
[Ref.: #25154]	adipate ChEBI	-	assimilation
[Ref.: #25154]	butanol ChEBI	-	assimilation
[Ref.: #25154]	cellulose ChEBI	-	hydrolysis
[Ref.: #25154]	citrate ChEBI	+	assimilation
[Ref.: #25154]	creatine ChEBI	+	assimilation
[Ref.: #25154]	D-fructose ChEBI	+	assimilation
[Ref.: #25154]	D-glucose ChEBI	-	assimilation
[Ref.: #25154]	D-mannose ChEBI	+	assimilation
[Ref.: #25154]	D-xylose ChEBI	-	assimilation
[Ref.: #25154]	decanoate ChEBI	-	assimilation
[Ref.: #25154]	esculin ChEBI	-	hydrolysis
[Ref.: #25154]	ethanol ChEBI	-	assimilation
[Ref.: #25154]	gelatin ChEBI	-	hydrolysis
[Ref.: #25154]	glucose ChEBI	-	fermentation
[Ref.: #25154]	L-arabinose ChEBI	-	assimilation
[Ref.: #25154]	L-arginine ChEBI	-	assimilation
[Ref.: #25154]	L-aspartate ChEBI	-	assimilation
[Ref.: #25154]	L-glutamate ChEBI	-	assimilation
[Ref.: #25154]	L-leucine ChEBI	-	assimilation
[Ref.: #25154]	L-phenylalanine ChEBI	-	assimilation
[Ref.: #25154]	L-proline ChEBI	+	assimilation
[Ref.: #25154]	L-rhamnose ChEBI	+	assimilation
[Ref.: #25154]	L-serine ChEBI	+	assimilation
[Ref.: #25154]	lactose ChEBI	+	assimilation
[Ref.: #25154]	malate ChEBI	+	assimilation
[Ref.: #25154]	malonate ChEBI	-	assimilation
[Ref.: #25154]	maltose ChEBI	-	assimilation
[Ref.: #25154]	mannitol ChEBI	-	assimilation
[Ref.: #25154]	mannitol ChEBI	+	assimilation
[Ref.: #25154]	methanol ChEBI	-	assimilation
[Ref.: #25154]	myo-inositol ChEBI	-	assimilation
[Ref.: #25154]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #25154]	nitrate ChEBI	-	reduction
[Ref.: #25154]	phenylacetate ChEBI	-	assimilation
[Ref.: #25154]	potassium gluconate ChEBI	-	assimilation
[Ref.: #25154]	sodium citrate ChEBI	+	assimilation
[Ref.: #25154]	starch ChEBI	-	hydrolysis
[Ref.: #25154]	sucrose ChEBI	-	assimilation
[Ref.: #25154]	tryptophan ChEBI	-	energy source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #25154]	amoxicillin ChEBI	yes	10	µg (disc)
[Ref.: #25154]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #25154]	bacitracin ChEBI				yes	0.04	Unit (disc)
[Ref.: #25154]	carbenicillin ChEBI				yes	100	µg (disc)
[Ref.: #25154]	cephradine ChEBI	yes	30	µg (disc)
[Ref.: #25154]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #25154]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #25154]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #25154]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #25154]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #25154]	penicillin g ChEBI	yes	10	Unit (disc)
[Ref.: #25154]	rifampicin ChEBI				yes	5	µg (disc)
[Ref.: #25154]	roxithromycin ChEBI	yes	15	µg (disc)
[Ref.: #25154]	spectinomycin ChEBI	yes	100	µg (disc)
[Ref.: #25154]	streptomycin ChEBI				yes	10	µg (disc)
[Ref.: #25154]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #25154]	trimethoprim ChEBI	yes	5	µg (disc)
[Ref.: #25154]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25154]		acetoin ChEBI	no
[Ref.: #25154]		indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #25154]	acetoin ChEBI	-
[Ref.: #25154]	glucose ChEBI		-
[Ref.: #25154]	indole ChEBI			-

	Enzyme	Enzyme activity	EC number
[Ref.: #25154]	acid phosphatase	+	3.1.3.2
[Ref.: #25154]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25154]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #25154]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25154]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25154]	alpha-glucosidase	-	3.2.1.20
[Ref.: #25154]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25154]	arginine dihydrolase	+	3.5.3.6
[Ref.: #25154]	beta-galactosidase	-	3.2.1.23
[Ref.: #25154]	beta-glucosidase	-	3.2.1.21
[Ref.: #25154]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25154]	catalase	+	1.11.1.6
[Ref.: #25154]	cystine arylamidase	-	3.4.11.3
[Ref.: #25154]	cytochrome oxidase	-	1.9.3.1
[Ref.: #25154]	esterase (C 4)	+
[Ref.: #25154]	esterase lipase (C 8)	+
[Ref.: #25154]	leucine arylamidase	+	3.4.11.1
[Ref.: #25154]	lipase (C 14)	-
[Ref.: #25154]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #25154]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25154]	trypsin	-	3.4.21.4
[Ref.: #25154]	urease	+	3.5.1.5
[Ref.: #25154]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25154]

Fatty acid	Percentage
C15:0 iso	39.3
C16:0 iso	12.2
C17:0 iso	11.1
C17:0 anteiso	7.1
C17:1 iso ω10c	6.6
C15:0 anteiso	6.1
C16:1ω7c alcohol	6.1
C16:1ω11c	2.3
C15:0	1.9
C17:1 iso I /C17:1 anteiso B	1.9
C18:0	0.8

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25154]	Sample type/isolated from	saline–alkaline soil
[Ref.: #25154]	Geographic location (country and/or sea, region)	Farmland in Hangjin Banner in Inner Mongolia
[Ref.: #25154]	Country	China
[Ref.: #25154]	Country ISO 3 Code	CHN
[Ref.: #25154]	Continent	Asia
[Ref.: #25154]	Geographic location	40.0000°/107.0000°
[Ref.: #25154]	Enrichment culture	Luria-Bertani agar (LB)
[Ref.: #25154]	Enrichment culture composition	tryptone 10 g/l, yeast extract 5 g/l, NaCl 10 g/l, agar 20 g/l, pH 7.0
[Ref.: #25154]	Enrichment culture duration	2 days
[Ref.: #25154]	Enrichment culture temperature	30 ̊C
[Ref.: #25154]	Isolation/enrichment procedure	10-fold dilution method
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Condition	#Alkaline	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX258757 (>99% sequence identity) for Lysinibacillus alkalisoli subclade from Microbeatlas

Aquatic Samples	82
Soil Samples	178
Animal Samples	289
Plant Samples	17
Total Samples	566

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25154]	Lysinibacillus sp. strain Y2A20 16S ribosomal RNA gene, partial sequence	KX258757	1347	GenBank	1911548 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lysinibacillus alkalisoli CGMCC 1.15760	GCA_014643595	contig	GenBank	1911548 tax ID	*
[Ref.: #66792]	Lysinibacillus alkalisoli strain CGMCC 1.15760	1911548.3	wgs	PATRIC	1911548 tax ID	*

[Ref.: #25154]

GC-content

39 mol%

Thermal denaturation, fluorometry

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lysinibacillus alkalisoli strain CGMCC 1.15760 PATRIC: 1911548.3
[Ref.: #69480]	motile	yes	92.478	yes
[Ref.: #69480]	gram-positive	yes	92.2	yes
[Ref.: #69480]	anaerobic	no	97.998	yes
[Ref.: #69480]	aerobic	yes	92.73	yes
[Ref.: #69480]	halophile	no	89.467	yes
[Ref.: #69480]	spore-forming	yes	95.424	yes
[Ref.: #69480]	glucose-util	yes	65.221	yes
[Ref.: #69480]	flagellated	yes	90.338	yes
[Ref.: #69480]	thermophile	no	99.332	no
[Ref.: #69480]	glucose-ferment	no	90.684	yes

Availability in culture collections External links

[Ref.: #25154]

Culture collection no.

CGMCC 1.15760, KCTC 33825

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lysinibacillus alkalisoli sp. nov., isolated from saline-alkaline soil.	Sun JQ, Xu L, Wu XL	Int J Syst Evol Microbiol	10.1099/ijsem.0.001571	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#25154

J. Q. X. Sun, L.,Wu, X. L.: Lysinibacillus alkalisoli sp. nov., isolated from saline-alkaline soil. IJSEM 67: 67 - 71 2017 ( DOI 10.1099/ijsem.0.001571 , PubMed 27902208 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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Lysinibacillus alkalisoli strain CGMCC 1.15760

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