Virgibacillus jeotgali (NS3012, KEMB 9001-125, JCM 16522)

Name: Virgibacillus jeotgali (NS3012, KEMB 9001-125, JCM 16522)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 133313

Type strain

Strain Designation NS3012

Culture col. no. NS3012 KEMB 9001-125 JCM 16522 DSM 107337 NRIC 0768

NCBI tax ID(s) 679900

Special Collections DSMZ JCM Literature strains

Links

Virgibacillus jeotgali NS3012 is an aerobe, spore-forming, Gram-positive prokaryote that forms circular colonies and was isolated from Myeolchi-jeotgal, a traditional Korean high-salt-fermented anchovy.

spore-forming Gram-positive motile rod-shaped colony-forming aerobe 16S sequence

Name and taxonomic classification

SI-ID 398705

JCM 16522,DSM 107337

@ref 20215

Last LPSN update 2025-12-16 10:01:45

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Virgibacillus

Species Virgibacillus jeotgali

Full scientific name Virgibacillus jeotgali Sundararaman et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
25166	positive	2.0-2.5 µm	0.5-0.7 µm	rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
25166	0.5-1.0 mm	pale yellow	circular	3 days	marine broth (MA)

Culture and growth conditions

Culture medium

@ref	Name	Growth
25166	Marine agar (MA)
64837	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
25166	nutrient agar (NA; Difco)
25166	R2A (Difco)
25166	LB (Luria-Bertani) MEDIUM

Culture temp

@ref	Growth	Type	Temperature (°C)
25166	positive	growth	10-40
25166	positive	optimum	28-30
25166	negative	other	50
64837	positive	growth	30
67770	positive	growth	30

Culture pH

@ref	Ability	Type	PH
25166	positive	optimum	7
25166	positive	growth	6.0-9.0

Physiology and metabolism

Oxygen tolerance

25166

Oxygen toleranceaerobe

Spore formation

@ref	Spore description	Type of spore	Spore formation
25166	ellipsoidal, centrally or subterminally with slightly swollen sporangia	endospore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
25166	NaCl	positive	growth	12 %(w/v)
25166	NaCl		optimum	2.0-3.0 %(w/v)

Observation

67770

Observationquinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
25166	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
25166	16808 ChEBI	2-dehydro-D-gluconate	+	assimilation
25166	16193 ChEBI	3-hydroxybenzoate	+	assimilation
25166	37054 ChEBI	3-hydroxybutyrate	+	assimilation
25166	17879 ChEBI	4-hydroxybenzoate	-	assimilation
25166	58143 ChEBI	5-dehydro-D-gluconate	-	assimilation
25166	58143 ChEBI	5-dehydro-D-gluconate	+/-	builds acid from
25166	30089 ChEBI	acetate	+	assimilation
25166	17128 ChEBI	adipate	-	assimilation
25166	28938 ChEBI	ammonium	+	nitrogen source
25166	27613 ChEBI	amygdalin	+/-	builds acid from
25166	18305 ChEBI	arbutin	+/-	builds acid from
25166	17968 ChEBI	butyrate	-	assimilation
25166		casein	+	hydrolysis
25166	17057 ChEBI	cellobiose	+	builds acid from
25166	16947 ChEBI	citrate	+	carbon source
25166	17108 ChEBI	D-arabinose	-	builds acid from
25166	18333 ChEBI	D-arabitol	-	builds acid from
25166	15824 ChEBI	D-fructose	+	builds acid from
25166	28847 ChEBI	D-fucose	+/-	builds acid from
25166	12936 ChEBI	D-galactose	+/-	builds acid from
25166	17634 ChEBI	D-glucose	+	assimilation
25166	17634 ChEBI	D-glucose	+	builds acid from
25166	62318 ChEBI	D-lyxose	-	builds acid from
25166	16899 ChEBI	D-mannitol	+	assimilation
25166	16899 ChEBI	D-mannitol	+	builds acid from
25166	16024 ChEBI	D-mannose	-	assimilation
25166	16024 ChEBI	D-mannose	+/-	builds acid from
25166	16988 ChEBI	D-ribose	+	assimilation
25166	16988 ChEBI	D-ribose	+	builds acid from
25166	17924 ChEBI	D-sorbitol	-	assimilation
25166	17924 ChEBI	D-sorbitol	-	builds acid from
25166	16443 ChEBI	D-tagatose	-	builds acid from
25166	65327 ChEBI	D-xylose	+	builds acid from
25166	17113 ChEBI	erythritol	+	builds acid from
25166	4853 ChEBI	esculin	+	builds acid from
25166	16813 ChEBI	galactitol	-	builds acid from
25166	28066 ChEBI	gentiobiose	-	builds acid from
25166	24265 ChEBI	gluconate	-	builds acid from
25166	24265 ChEBI	gluconate	+	assimilation
25166	17754 ChEBI	glycerol	+	builds acid from
25166	28087 ChEBI	glycogen	-	assimilation
25166	28087 ChEBI	glycogen	-	builds acid from
25166	15443 ChEBI	inulin	+/-	builds acid from
25166	17240 ChEBI	itaconate	-	assimilation
25166	16977 ChEBI	L-alanine	+	assimilation
25166	30849 ChEBI	L-arabinose	+	assimilation
25166	30849 ChEBI	L-arabinose	+	builds acid from
25166	18403 ChEBI	L-arabitol	-	builds acid from
25166	18287 ChEBI	L-fucose	-	assimilation
25166	18287 ChEBI	L-fucose	-	builds acid from
25166	15971 ChEBI	L-histidine	+	assimilation
25166	15589 ChEBI	L-malate	+	assimilation
25166	17203 ChEBI	L-proline	+	assimilation
25166	62345 ChEBI	L-rhamnose	-	assimilation
25166	62345 ChEBI	L-rhamnose	-	builds acid from
25166	17115 ChEBI	L-serine	-	assimilation
25166	17266 ChEBI	L-sorbose	-	builds acid from
25166	65328 ChEBI	L-xylose	-	builds acid from
25166	24996 ChEBI	lactate	+	assimilation
25166	17716 ChEBI	lactose	-	builds acid from
25166	15792 ChEBI	malonate	-	assimilation
25166	17306 ChEBI	maltose	+	assimilation
25166	17306 ChEBI	maltose	+	builds acid from
25166	6731 ChEBI	melezitose	-	builds acid from
25166	28053 ChEBI	melibiose	-	assimilation
25166	28053 ChEBI	melibiose	-	builds acid from
25166	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from
25166	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
25166	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
25166	17268 ChEBI	myo-inositol	+	builds acid from
25166	17268 ChEBI	myo-inositol	+	assimilation
25166	506227 ChEBI	N-acetylglucosamine	-	builds acid from
25166	506227 ChEBI	N-acetylglucosamine	+	assimilation
25166	17632 ChEBI	nitrate	+	reduction
25166	18401 ChEBI	phenylacetate	+	assimilation
25166	17272 ChEBI	propionate	-	assimilation
25166	16634 ChEBI	raffinose	+/-	builds acid from
25166	15963 ChEBI	ribitol	-	builds acid from
25166	17814 ChEBI	salicin	-	assimilation
25166	17814 ChEBI	salicin	+	builds acid from
25166	28017 ChEBI	starch	+/-	builds acid from
25166	9300 ChEBI	suberic acid	-	assimilation
25166	17992 ChEBI	sucrose	+	assimilation
25166	17992 ChEBI	sucrose	+	builds acid from
25166	27082 ChEBI	trehalose	+	builds acid from
25166	27897 ChEBI	tryptophan	-	energy source
25166	32528 ChEBI	turanose	-	builds acid from
25166	53426 ChEBI	tween 80	+	hydrolysis
25166	31011 ChEBI	valerate	+	assimilation
25166	17151 ChEBI	xylitol	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
25166	16136 ChEBI	hydrogen sulfide
25166	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Citrate test
25166	16947 ChEBI	citrate	+
25166	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
25166	acid phosphatase	+	3.1.3.2
25166	alkaline phosphatase	+	3.1.3.1
25166	alpha-chymotrypsin	+	3.4.21.1
25166	alpha-fucosidase	-	3.2.1.51
25166	alpha-galactosidase	-	3.2.1.22
25166	alpha-glucosidase	+	3.2.1.20
25166	alpha-mannosidase	-	3.2.1.24
25166	arginine dihydrolase	-	3.5.3.6
25166	beta-galactosidase	-	3.2.1.23
25166	beta-glucosidase	+	3.2.1.21
25166	beta-glucuronidase	-	3.2.1.31
25166	catalase	+	1.11.1.6
25166	cystine arylamidase	-	3.4.11.3
25166	cytochrome oxidase	+	1.9.3.1
25166	esterase (C 4)	+
25166	esterase Lipase (C 8)	+
25166	gelatinase	+
25166	leucine arylamidase	+	3.4.11.1
25166	lipase (C 14)	-
25166	N-acetyl-beta-glucosaminidase	-	3.2.1.52
25166	naphthol-AS-BI-phosphohydrolase	+
25166	trypsin	-	3.4.21.4
25166	tryptophan deaminase	-	4.1.99.1
25166	urease	-	3.5.1.5
25166	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

@ref

25166

Fatty acid	Percentage
C15:0 anteiso	79.5
C17:0 anteiso	13.4
C15:0 iso	1.5
C16:0	1.2
C16:0 iso	1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Seafood
#Engineered	#Food production	#Fermented

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture duration	Enrichment culture temperature
25166	Myeolchi-jeotgal, a traditional Korean high-salt-fermented anchovy	Republic of Korea	KOR	Asia	marine agar (MA; Difco)	7 days	20
64837	Myeolchi-jeotgal, a traditional Korean fermented anchovy	Republic of Korea	KOR	Asia
67770	Myeolchi-jeotgal, a traditional Korean high-salt-fermented anchovy

Taxonmaps

69479

Global distribution of 16S sequence GQ889491 (>99% sequence identity) for Virgibacillus jeotgali subclade from Microbeatlas

Aquatic Samples	12
Soil Samples	36
Animal Samples	55
Plant Samples	7
Total Samples	110

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
64837	1	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64837	Virgibacillus jeotgali strain NS3012 16S ribosomal RNA gene, partial sequence	GQ889491	1416		679900

GC content

@ref	GC-content (mol%)	Method
25166	36.8	high performance liquid chromatography (HPLC)
64837	36.8

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Virgibacillus jeotgali sp. nov., isolated from Myeolchi-jeotgal, a traditional Korean high-salt-fermented anchovy.	Sundararaman A, Srinivasan S, Lee JH, Lee SS	Int J Syst Evol Microbiol	10.1099/ijsem.0.001596	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25166	A. S. Sundararaman, Sathiyaraj,Lee, Jong-Hoon,Lee, Sang-Seob: Virgibacillus jeotgali sp. nov., isolated from Myeolchi-jeotgal, a traditional Korean high-salt-fermented anchovy. IJSEM 67: 158 - 163 2017 ( DOI 10.1099/ijsem.0.001596 , PubMed 27902213 )
#64837	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107337
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Virgibacillus jeotgali (NS3012, KEMB 9001-125, JCM 16522)

Name and taxonomic classification