Name: Lentzea californiensis DSM 43393
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13310

Type strain:

Species: Lentzea californiensis

Culture col. no.: DSM 43393, IMRU 550, NRRL B-16137, JCM 11305, BCRC 16972, CGMCC 4.2094, KCTC 19912

Strain history: NRRL B-16137 <-- IMRU 550 <-- E. N. Azarowicz.

NCBI tax ID(s): 438851 (species)

SI-ID 50267

DSM 43393, NRRL B-16137, KCTC 19912, JCM 11305, CGMCC 4.2094

Special Collections

Section

Lentzea californiensis DSM 43393 is a bacterium that builds an aerial mycelium and was isolated from soil.

16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Lentzea*
	Species	**Lentzea californiensis**
	Full Scientific Name (LPSN)	Lentzea californiensis Labeda et al. 2001

Information on morphological and physiological properties Morphology

[Ref.: #19571]	Colony color	sun yellow
[Ref.: #19571]	Incubation period	10-14 days
[Ref.: #19571]	Cultivation medium used	ISP 2

[Ref.: #19571]	Colony color	sun yellow
[Ref.: #19571]	Incubation period	10-14 days
[Ref.: #19571]	Cultivation medium used	ISP 3

[Ref.: #19571]	Colony color	beige
[Ref.: #19571]	Incubation period	10-14 days
[Ref.: #19571]	Cultivation medium used	ISP 4

[Ref.: #19571]	Colony color	beige
[Ref.: #19571]	Incubation period	10-14 days
[Ref.: #19571]	Cultivation medium used	ISP 5

[Ref.: #19571]	Colony color	sun yellow
[Ref.: #19571]	Incubation period	10-14 days
[Ref.: #19571]	Cultivation medium used	ISP 6

[Ref.: #19571]	Colony color	beige
[Ref.: #19571]	Incubation period	10-14 days
[Ref.: #19571]	Cultivation medium used	ISP 7

[Ref.: #19571]	Multicellular complex forming ability	yes
[Ref.: #19571]	Multicellular complex name	Aerial Mycelium
[Ref.: #19571]	Multicellular complex color	White
[Ref.: #19571]	Medium Name (multicellularity)	ISP 2

[Ref.: #19571]	Multicellular complex forming ability	yes
[Ref.: #19571]	Multicellular complex name	Aerial Mycelium
[Ref.: #19571]	Multicellular complex color	White
[Ref.: #19571]	Medium Name (multicellularity)	ISP 3

[Ref.: #19571]	Multicellular complex forming ability	yes
[Ref.: #19571]	Multicellular complex name	Aerial Mycelium
[Ref.: #19571]	Multicellular complex color	White
[Ref.: #19571]	Medium Name (multicellularity)	ISP 4

[Ref.: #19571]	Multicellular complex forming ability	no
[Ref.: #19571]	Medium Name (multicellularity)	ISP 5

[Ref.: #19571]	Multicellular complex forming ability	no
[Ref.: #19571]	Medium Name (multicellularity)	ISP 6

[Ref.: #19571]	Multicellular complex forming ability	no
[Ref.: #19571]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11008]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11008]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #11008]

Culture medium composition

expand / minimize

[Ref.: #19571]

Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #19571]

Culture medium

ISP 5

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Culture medium growth

[Ref.: #19571]

Culture medium composition

expand / minimize

[Ref.: #19571]

Culture medium

ISP 6

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Culture medium growth

[Ref.: #19571]

Culture medium composition

expand / minimize

[Ref.: #19571]

Culture medium

ISP 7

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #11008]	growth	28 °C
[Ref.: #19571]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #19571]	arabinose ChEBI	+
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19571]	cellulose ChEBI	+
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #19571]	fructose ChEBI	+
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19571]	glucose ChEBI	+
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #19571]	mannitol ChEBI	+
[Ref.: #19571]	myo-inositol ChEBI	+
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #19571]	raffinose ChEBI	+
[Ref.: #19571]	rhamnose ChEBI	+/-
[Ref.: #19571]	sucrose ChEBI	+
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19571]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E, API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19571]	422	+	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19571]	408	not determinedn.d.	+	-	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11008]	Sample type/isolated from	soil
[Ref.: #11008]	Geographic location (country and/or sea, region)	California
[Ref.: #11008]	Country	USA
[Ref.: #11008]	Country ISO 3 Code	USA
[Ref.: #11008]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	CA
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC214996 (>99% sequence identity) for Lentzea albidocapillata from Microbeatlas

Aquatic Samples	19
Soil Samples	500
Animal Samples	45
Plant Samples	246
Total Samples	810

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11008]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19571]	Biosafety level	1	Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lentzea californiensis gene for 16S ribosomal RNA, partial sequence, culture_collection: JCM:11305	LC214996	1455	GenBank ENA	438851 tax ID
[Ref.: #20218]	Lentzea californiensis strain NRRL B-16137 16S ribosomal RNA gene, partial sequence	AF174435	1451	GenBank ENA	438851 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lentzea californiensis DSM 43393	2596583533	draft	JGI IMG/M	438851 tax ID	*
[Ref.: #66792]	Lentzea californiensis DSM 43393	GCA_024648825	contig	GenBank ENA	438851 tax ID	*

[Ref.: #67770]

GC-content

69.3 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #11008]

Culture collection no.

DSM 43393, IMRU 550, NRRL B-16137, JCM 11305, BCRC 16972, CGMCC 4.2094, KCTC 19912

[Ref.: #82510]

SI-ID 50267

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lentzea indica sp. nov., a novel actinobacteria isolated from Indian Himalayan-soil.	Maiti PK, Mandal S	Antonie Van Leeuwenhoek	10.1007/s10482-020-01449-8	2020	*
Phylogeny	Revival of the genus Lentzea and proposal for Lechevalieria gen. nov.	Labeda DP, Hatano K, Kroppenstedt RM, Tamura T	Int J Syst Evol Microbiol	10.1099/00207713-51-3-1045	2001	*

References

#11008

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43393

#19571

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82510

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID50267.1 )

* These data were automatically processed and therefore are not curated

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