Acidobacterium ailaaui (DSM 27394, LMG 28340, PMMR2)

Name: Acidobacterium ailaaui (DSM 27394, LMG 28340, PMMR2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 133033

Type strain

Strain Designation PMMR2

Culture col. no. DSM 27394 LMG 28340 PMMR2

NCBI tax ID(s) 1382359

Special Collections DSMZ Literature strains

History DSMZ <- G. King, Louisiana State Univ., Baton Rouge, USA; PMMR2 <- M. Russell

Links

Acidobacterium ailaaui PMMR2 is an aerobe, chemoorganotroph, Gram-negative bacterium that forms circular colonies and was isolated from geothermally heated microbial mat.

Gram-negative rod-shaped colony-forming aerobe chemoorganotroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 399004

LMG 28340,DSM 27394

@ref 20215

Last LPSN update 2026-05-29 08:38:00

Domain Bacteria

Phylum Acidobacteriota

Class Terriglobia

Order Terriglobales

Family Acidobacteriaceae

Genus Acidobacterium

Species Acidobacterium ailaaui

Full scientific name Acidobacterium ailaaui Myers and King 2016

Synonyms (1)

Pseudacidobacterium ailaaui

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
25069	negative	1.3-1.7 µm	0.7-0.9 µm	rod-shaped

Colony morphology

@ref	Colony color	Colony shape	Incubation period	Medium used
25069	light beige	circular	6 days	R2A agar (Reasoner's 2A agar; pH 5.5)

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
25069	R2A agar (Reasoner's 2A agar; pH 5.5)
24821	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
24821	positive	growth	40
25069	positive	growth	15.0-55.0
25069	positive	optimum	40

Culture pH

@ref	Ability	Type	PH
25069	positive	optimum	6.5
25069	positive	growth	4.5-7.0

Physiology and metabolism

Oxygen tolerance

25069

Oxygen toleranceaerobe

Nutrition type

25069

Typechemoorganotroph

Spore formation

25069

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
25069	NaCl		growth	1 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
25069	17824 ChEBI	2-propanol	-	carbon source
25069	30089 ChEBI	acetate	-	carbon source
25069	15347 ChEBI	acetone	-	carbon source
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
25069	16449 ChEBI	alanine	-	carbon source
25069	28938 ChEBI	ammonium	+	nitrogen source
25069	22599 ChEBI	arabinose	+	carbon source
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
25069	35391 ChEBI	aspartate	-	carbon source
25069	17750 ChEBI	betaine	-	carbon source
25069	8391 ChEBI	D-gluconate	+	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
25069	15748 ChEBI	D-glucuronate	+	carbon source
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
25069	17170 ChEBI	dimethylamine	-	carbon source
25069	4853 ChEBI	esculin	+	hydrolysis
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
25069	16236 ChEBI	ethanol	-	carbon source
25069	28757 ChEBI	fructose	+	carbon source
25069	29806 ChEBI	fumarate	-	carbon source
25069	28260 ChEBI	galactose	+	carbon source
25069	5291 ChEBI	gelatin	+	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
25069	17234 ChEBI	glucose	-	fermentation
25069	17234 ChEBI	glucose	+	carbon source
25069	33871 ChEBI	glycerate	-	carbon source
25069	17754 ChEBI	glycerol	+	carbon source
25069	15428 ChEBI	glycine	-	carbon source
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
25069	24996 ChEBI	lactate	-	carbon source
25069	17716 ChEBI	lactose	+	carbon source
25069	25115 ChEBI	malate	-	carbon source
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
25069	15792 ChEBI	malonate	-	carbon source
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
25069	37684 ChEBI	mannose	+	carbon source
25069	17790 ChEBI	methanol	-	carbon source
25069	16830 ChEBI	methylamine	-	carbon source
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
25069	17632 ChEBI	nitrate	-	reduction
25069	17632 ChEBI	nitrate	+	nitrogen source
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
25069	26271 ChEBI	proline	+	carbon source
25069	17272 ChEBI	propionate	-	carbon source
25069	15361 ChEBI	pyruvate	-	carbon source
25069	33942 ChEBI	ribose	-	carbon source
25069	17822 ChEBI	serine	-	carbon source
25069	30031 ChEBI	succinate	+	carbon source
25069	17992 ChEBI	sucrose	+	carbon source
25069	132950 ChEBI	tartrate	-	carbon source
25069	18139 ChEBI	trimethylamine	-	carbon source
25069	27897 ChEBI	tryptophan	-	energy source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
25069	27266 ChEBI	valine	-	carbon source
25069	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
25069	35581 ChEBI	indole
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
25069	35581 ChEBI	indole	-
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	+	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
25069	arginine dihydrolase	-	3.5.3.6
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
25069	beta-galactosidase	-	3.2.1.23
25069	beta-glucosidase	-	3.2.1.21
68382	beta-glucosidase	+	3.2.1.21	from API zym
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
25069	catalase	-	1.11.1.6
25069	cytochrome oxidase	-	1.9.3.1
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	trypsin	+	3.4.21.4	from API zym
25069	tryptophan deaminase	-	4.1.99.1
25069	urease	+	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
24821	-	+	-	-	-	+	+	+/-	+	-	+/-	+/-	-	+/-	+/-	-	+	+	-	+

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
24821	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.
24821	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Microbial community	#Microbial mat

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture temperature
24821	geothermally heated microbial mat	Hawaii, Kilauea Volcano, Puhimau geothermal area	USA	USA	North America
25069						1/10 R2A (pH 4) agar plates	30

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24821	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM68845v1 assembly for Pseudacidobacterium ailaaui PMMR2	contig	GCA_000688455	1382359.3	2558860986	1382359	78.48

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24821	Pseudacidobacterium ailaaui strain PMMR2 16S ribosomal RNA gene, partial sequence	KX306477	1258		1382359
124043	Pseudacidobacterium ailaaui strain DSM 27394 16S ribosomal RNA gene, partial sequence.	PP836138	1500		1382359

GC content

@ref	GC-content (mol%)	Method
25069	57.2	high performance liquid chromatography (HPLC)
25069	56.5	genome sequence analysis
24821	52.7	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM68845v1 assembly for Pseudacidobacterium ailaaui PMMR2
NCBI: GCA_000688455

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	82.60	no
125439	gram_stain	BacteriaNetⓘ	negative	78.17	no
125439	motility	BacteriaNetⓘ	yes	50.49	no
125439	spore_formation	BacteriaNetⓘ	no	86.68	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paracidobacterium acidisoli gen. nov., sp. nov. and Alloacidobacterium dinghuense gen. nov., sp. nov., two acidobacteria isolated from forest soil, and reclassification of Acidobacterium ailaaui and Acidipila dinghuensis as Pseudacidobacterium ailaaui gen. nov., comb. nov. and Silvibacterium dinghuense comb. nov.	Zhang QM, Fu JC, Chen ZQ, Qiu LH	Int J Syst Evol Microbiol	10.1099/ijsem.0.005415	2022
Phylogeny	Isolation and characterization of Acidobacterium ailaaui sp. nov., a novel member of Acidobacteria subdivision 1, from a geothermally heated Hawaiian microbial mat.	Myers MR, King GM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001516	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24821	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27394
#25069	M. R. K. Myers, G. M.: Isolation and characterization of Acidobacterium ailaaui sp. nov., a novel member of Acidobacteria subdivision 1, from a geothermally heated Hawaiian microbial mat. IJSEM 66: 5328 - 5335 2016 ( DOI 10.1099/ijsem.0.001516 , PubMed 27692038 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Acidobacterium ailaaui (DSM 27394, LMG 28340, PMMR2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000688455

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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