Name: Acidobacterium ailaaui PMMR2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133033

Type strain:

Species: Acidobacterium ailaaui

Strain Designation: PMMR2

Culture col. no.: DSM 27394, LMG 28340

Strain history: <- G. King, Louisiana State Univ., Baton Rouge, USA; PMMR2 <- M. Russell

NCBI tax ID(s): 1382359 (species)

SI-ID 399004

DSM 27394

Section

Acidobacterium ailaaui PMMR2 is an aerobe, chemoorganotroph, Gram-negative bacterium that forms circular colonies and was isolated from geothermally heated microbial mat.

colony-forming
Gram-negative
rod-shaped
aerobe
chemoorganotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Acidobacteriota*
	Class	*Terriglobia*
	Order	*Terriglobales*
	Family	*Acidobacteriaceae*
	Genus	*Acidobacterium*
	Species	**Acidobacterium ailaaui**
	Full Scientific Name (LPSN)	Acidobacterium ailaaui Myers and King 2016

Synonym

Pseudacidobacterium ailaaui

Information on morphological and physiological properties Morphology

[Ref.: #25069]	Gram stain	negative

[Ref.: #25069]	Cell length	1.3-1.7 µm

[Ref.: #25069]	Cell width	0.7-0.9 µm

[Ref.: #25069]	Cell shape	rod-shaped

[Ref.: #25069]	Motility	no

[Ref.: #25069]	Colony color	light beige
[Ref.: #25069]	Colony shape	circular
[Ref.: #25069]	Incubation period	6 days
[Ref.: #25069]	Cultivation medium used	R2A agar (Reasoner's 2A agar; pH 5.5)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25069]

Culture medium

R2A agar (Reasoner's 2A agar; pH 5.5)

[Ref.: #25069]

Culture medium growth

[Ref.: #24821]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #24821]

Culture medium growth

[Ref.: #24821]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24821]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24821]	growth	40 °C
[Ref.: #25069]	growth	15.0-55.0 °C
[Ref.: #25069]	optimum	40 °C

[Ref.: #24821]	Temperature range	thermophilic
[Ref.: #25069]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #25069]			optimum	6.5
[Ref.: #25069]			growth	4.5-7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25069]

Oxygen tolerance

aerobe

[Ref.: #25069]

Nutrition type

chemoorganotroph

[Ref.: #25069]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25069]		NaCl	no	growth	1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25069]	2-propanol ChEBI	-	carbon source
[Ref.: #25069]	acetate ChEBI	-	carbon source
[Ref.: #25069]	acetone ChEBI	-	carbon source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #25069]	alanine ChEBI	-	carbon source
[Ref.: #25069]	ammonium ChEBI	+	nitrogen source
[Ref.: #25069]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #25069]	aspartate ChEBI	-	carbon source
[Ref.: #25069]	betaine ChEBI	-	carbon source
[Ref.: #25069]	D-gluconate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #25069]	D-glucuronate ChEBI	+	carbon source
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #25069]	dimethylamine ChEBI	-	carbon source
[Ref.: #25069]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #25069]	ethanol ChEBI	-	carbon source
[Ref.: #25069]	fructose ChEBI	+	carbon source
[Ref.: #25069]	fumarate ChEBI	-	carbon source
[Ref.: #25069]	galactose ChEBI	+	carbon source
[Ref.: #25069]	gelatin ChEBI	+	hydrolysis
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #25069]	glucose ChEBI	-	fermentation
[Ref.: #25069]	glucose ChEBI	+	carbon source
[Ref.: #25069]	glycerate ChEBI	-	carbon source
[Ref.: #25069]	glycerol ChEBI	+	carbon source
[Ref.: #25069]	glycine ChEBI	-	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #25069]	lactate ChEBI	-	carbon source
[Ref.: #25069]	lactose ChEBI	+	carbon source
[Ref.: #25069]	malate ChEBI	-	carbon source
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #25069]	malonate ChEBI	-	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #25069]	mannose ChEBI	+	carbon source
[Ref.: #25069]	methanol ChEBI	-	carbon source
[Ref.: #25069]	methylamine ChEBI	-	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #25069]	nitrate ChEBI	-	reduction
[Ref.: #25069]	nitrate ChEBI	+	nitrogen source
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #25069]	proline ChEBI	+	carbon source
[Ref.: #25069]	propionate ChEBI	-	carbon source
[Ref.: #25069]	pyruvate ChEBI	-	carbon source
[Ref.: #25069]	ribose ChEBI	-	carbon source
[Ref.: #25069]	serine ChEBI	-	carbon source
[Ref.: #25069]	succinate ChEBI	+	carbon source
[Ref.: #25069]	sucrose ChEBI	+	carbon source
[Ref.: #25069]	tartrate ChEBI	-	carbon source
[Ref.: #25069]	trimethylamine ChEBI	-	carbon source
[Ref.: #25069]	tryptophan ChEBI	-	energy source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #25069]	valine ChEBI	-	carbon source
[Ref.: #25069]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25069]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #25069]		indole ChEBI	-
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #25069]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #25069]	beta-galactosidase	-	3.2.1.23
[Ref.: #25069]	beta-glucosidase	-	3.2.1.21
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE, API zym
[Ref.: #25069]	catalase	-	1.11.1.6
[Ref.: #25069]	cytochrome oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #25069]	tryptophan deaminase	-	4.1.99.1
[Ref.: #25069]	urease	+	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #24821]	24738	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.
[Ref.: #24821]	24295	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #24821]	14086	-	+	-	-	-	+	+	+/-	+	-	+/-	+/-	-	+/-	+/-	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24821]	Sample type/isolated from	geothermally heated microbial mat
[Ref.: #24821]	Geographic location (country and/or sea, region)	Hawaii, Kilauea Volcano, Puhimau geothermal area
[Ref.: #24821]	Country	USA
[Ref.: #24821]	Country ISO 3 Code	USA
[Ref.: #24821]	Continent	North America

[Ref.: #25069]	Enrichment culture	1/10 R2A (pH 4) agar plates
[Ref.: #25069]	Enrichment culture temperature	30 ̊C
* marker position based on {}

Isolation sources categories

#Environmental

#Microbial community

#Microbial mat

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24821]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24821]	Acidobacterium ailaaui strain PMMR2 16S ribosomal RNA gene, partial sequence	KX306477	1258	ENA	1382359 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudacidobacterium ailaaui PMMR2	GCA_000688455	contig	GenBank	1382359 tax ID	*
[Ref.: #66792]	Acidobacterium ailaaui PMMR2	2558860986	draft	JGI IMG/M	1382359 tax ID	*

[Ref.: #25069]	GC-content	57.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #25069]	GC-content	56.5 mol%	genome sequence analysis
[Ref.: #24821]	GC-content	52.7 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24821]

Culture collection no.

DSM 27394, LMG 28340

[Ref.: #91303]

SI-ID 399004

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paracidobacterium acidisoli gen. nov., sp. nov. and Alloacidobacterium dinghuense gen. nov., sp. nov., two acidobacteria isolated from forest soil, and reclassification of Acidobacterium ailaaui and Acidipila dinghuensis as Pseudacidobacterium ailaaui gen. nov., comb. nov. and Silvibacterium dinghuense comb. nov.	Zhang QM, Fu JC, Chen ZQ, Qiu LH	Int J Syst Evol Microbiol	10.1099/ijsem.0.005415	2022	*
Phylogeny	Isolation and characterization of Acidobacterium ailaaui sp. nov., a novel member of Acidobacteria subdivision 1, from a geothermally heated Hawaiian microbial mat.	Myers MR, King GM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001516	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24821

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27394

#25069

M. R. K. Myers, G. M.: Isolation and characterization of Acidobacterium ailaaui sp. nov., a novel member of Acidobacteria subdivision 1, from a geothermally heated Hawaiian microbial mat. IJSEM 66: 5328 - 5335 2016 ( DOI 10.1099/ijsem.0.001516 , PubMed 27692038 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#91303

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399004.1 )

* These data were automatically processed and therefore are not curated

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