Name: Allofrancisella guangzhouensis 08HL01032
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132684

Type strain:

Species: Allofrancisella guangzhouensis

Strain Designation: 08HL01032

Culture col. no.: DSM 102975, ATCC BAA-2361, CCUG 60119, NCTC 13503

Strain history: <- NCTC 13503 <- P.-H. Qu, Guanhzhou CDC; 08HL01032

NCBI tax ID(s): 594679 (species)

SI-ID 396713

DSM 102975, CCUG 60119

Special Collections

Section

Allofrancisella guangzhouensis 08HL01032 is a microaerophile, Gram-negative, ovoid-shaped bacterium that was isolated from water, air condition system .

Gram-negative
ovoid-shaped
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Beggiatoales*
	Family	*Francisellaceae*
	Genus	*Allofrancisella*
	Species	**Allofrancisella guangzhouensis**
	Full Scientific Name (LPSN)	Allofrancisella guangzhouensis (Qu et al. 2013) Qu et al. 2016

Synonym

Francisella guangzhouensis

Information on morphological and physiological properties Morphology

[Ref.: #31018]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %97

[Ref.: #31018]	Cell shape	ovoid-shaped

[Ref.: #31018]	Motility	no

[Ref.: #62214]

Incubation period

1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24473]

Culture medium

COLUMBIA-GLUCOSE-CYSTEINE-AGAR (DSMZ Medium 429c)

[Ref.: #24473]

Culture medium growth

[Ref.: #24473]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24473]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24473]	growth	30 °C
[Ref.: #25033]	optimum	25-28 °C
[Ref.: #62214]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #62214]

Oxygen tolerance

microaerophile

[Ref.: #31018]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %95.442
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25033]	D-glucose ChEBI	+	fermentation
[Ref.: #25033]	esculin ChEBI	-	hydrolysis
[Ref.: #31018]	glucose ChEBI	+	carbon source
[Ref.: #31018]	maltose ChEBI	+	carbon source
[Ref.: #31018]	mannose ChEBI	+	carbon source
[Ref.: #25033]	nitrate ChEBI	-	reduction
[Ref.: #25033]	raffinose ChEBI	-	fermentation
[Ref.: #25033]	sucrose ChEBI	+	fermentation
[Ref.: #31018]	sucrose ChEBI	+	carbon source
[Ref.: #25033]	tryptophan ChEBI	-	energy source

	Metabolite production	Metabolite	Production
[Ref.: #25033]		indole ChEBI	no

	Physiological tests	Metabolite	Indole test
[Ref.: #25033]		indole ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #25033]	6-phospho-beta-galactosidase	-	3.2.1.85
[Ref.: #25033]	acid phosphatase	+	3.1.3.2
[Ref.: #31018]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #25033]	alanine arylamidase	+	3.4.11.2
[Ref.: #31018]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25033]	alpha-arabinosidase	-	3.2.1.55
[Ref.: #25033]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #25033]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #25033]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #25033]	alpha-glucosidase	-	3.2.1.20
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #25033]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #25033]	arginine arylamidase	+
[Ref.: #25033]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #25033]	beta-glucosidase	-	3.2.1.21
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #25033]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #25033]	catalase	+	1.11.1.6
[Ref.: #31018]	catalase	+	1.11.1.6
[Ref.: #25033]	cystine arylamidase	-	3.4.11.3
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #25033]	esterase (C 4)	+
[Ref.: #25033]	esterase lipase (C 8)	+
[Ref.: #25033]	glycin arylamidase	-
[Ref.: #25033]	histidine arylamidase	-
[Ref.: #25033]	leucine arylamidase	-	3.4.11.1
[Ref.: #25033]	leucyl glycin arylamidase	-	3.4.11.1
[Ref.: #25033]	lipase (C 14)	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #25033]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25033]	phenylalanine arylamidase	-
[Ref.: #25033]	proline-arylamidase	-	3.4.11.5
[Ref.: #25033]	pyroglutamic acid arylamidase	-
[Ref.: #25033]	serine arylamidase	-
[Ref.: #25033]	trypsin	-	3.4.21.4
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #25033]	tyrosine arylamidase	+
[Ref.: #25033]	urease	-	3.5.1.5
[Ref.: #25033]	valine arylamidase	-
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #24473]	13999	-	+/-	+/-	+/-	-	+/-	-	-	-	-	+	+/-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24473]	Sample type/isolated from	water, air condition system (cooling tower)
[Ref.: #24473]	Geographic location (country and/or sea, region)	Guangzhou
[Ref.: #24473]	Country	China
[Ref.: #24473]	Country ISO 3 Code	CHN
[Ref.: #24473]	Continent	Asia

[Ref.: #62214]	Sample type/isolated from	Water,air condition system
[Ref.: #62214]	Sampling date	2008-08-01
[Ref.: #62214]	Geographic location (country and/or sea, region)	Guangzhou CDC,Guangzhou
[Ref.: #62214]	Country	China
[Ref.: #62214]	Country ISO 3 Code	CHN
[Ref.: #62214]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Air conditioner
#Engineered	#Industrial	#Cooling tower
#Environmental	#Aquatic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence CP010427 (>99% sequence identity) for Allofrancisella guangzhouensis from Microbeatlas

Aquatic Samples	144
Soil Samples	17
Animal Samples	5
Plant Samples
Total Samples	166

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24473]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24473]	Francisella guangzhouensis strain 08HL01032 16S ribosomal RNA gene, partial sequence	FJ591095	1449	GenBank ENA	594679 tax ID
[Ref.: #25033]	Allofrancisella guangzhouensis strain 08HL01032 chromosome, complete genome	CP010427		GenBank ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Allofrancisella guangzhouensis 08HL01032	GCA_000815225	complete	GenBank ENA	594679 tax ID	*
[Ref.: #66792]	Allofrancisella guangzhouensis 08HL01032	2718217988	complete	JGI IMG/M	594679 tax ID	*

[Ref.: #25033]	GC-content	32.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #31018]	GC-content	32.5 mol%
[Ref.: #24473]	GC-content	32.5 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Allofrancisella guangzhouensis 08HL01032 NCBI: GCA_000815225.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Allofrancisella guangzhouensis 08HL01032 NCBI: GCA_000815225.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	97	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	93.276	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	55.299	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	95.442	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.519	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	87.528	no

Availability in culture collections External links

[Ref.: #24473]

Culture collection no.

DSM 102975, ATCC BAA-2361, CCUG 60119, NCTC 13503

[Ref.: #90992]

SI-ID 396713

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Characterization of Francisella species isolated from the cooling water of an air conditioning system.	Gu Q, Li X, Qu P, Hou S, Li J, Atwill ER, Chen S	Braz J Microbiol	10.1590/S1517-838246320140465	2015	*
Phylogeny	Francisella guangzhouensis sp. nov., isolated from air-conditioning systems.	Qu PH, Chen SY, Scholz HC, Busse HJ, Gu Q, Kampfer P, Foster JT, Glaeser SP, Chen C, Yang ZC	Int J Syst Evol Microbiol	10.1099/ijs.0.049916-0	2013	*
Phylogeny	Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov.	Qu PH, Li Y, Salam N, Chen SY, Liu L, Gu Q, Fang BZ, Xiao M, Li M, Chen C, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001437	2016	*
Genetics	Complete Genome Sequence of Francisella guangzhouensis Strain 08HL01032T, Isolated from Air-Conditioning Systems in China.	Svensson D, Ohrman C, Backman S, Karlsson E, Nilsson E, Bystrom M, Larkeryd A, Myrtennas K, Stenberg P, Qu PH, Trygg J, Scholz HC, Forsman M, Sjodin A	Genome Announc	10.1128/genomeA.00024-15	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24473

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102975

#25033

P. H. L. Qu, Y.,Salam, N.,Chen, S. Y.,Liu, L.,Gu, Q.,Fang, B. Z.,Xiao, M.,Li, M.,Chen, C.,Li, W. J.: Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov. IJSEM 66: 4832 - 4838 2016 ( DOI 10.1099/ijsem.0.001437 , PubMed 27543089 )

#31018

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27348 (see below)

#62214 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 60119

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90992

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396713.1 )

#27348

IJSEM 3628 2013 ( DOI 10.1099/ijs.0.049916-0 , PubMed 23606480 )

* These data were automatically processed and therefore are not curated

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