Strain identifier

BacDive ID: 132684

Type strain: Yes

Species: Allofrancisella guangzhouensis

Strain Designation: 08HL01032

Strain history: <- NCTC 13503 <- P.-H. Qu, Guanhzhou CDC; 08HL01032

NCBI tax ID(s): 594679 (species)

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General

@ref: 24473

BacDive-ID: 132684

DSM-Number: 102975

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, ovoid-shaped

description: Allofrancisella guangzhouensis 08HL01032 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from water, air condition system .

NCBI tax id

  • NCBI tax id: 594679
  • Matching level: species

strain history

  • @ref: 24473
  • history: <- NCTC 13503 <- P.-H. Qu, Guanhzhou CDC; 08HL01032

doi: 10.13145/bacdive132684.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Beggiatoales
  • family: Francisellaceae
  • genus: Allofrancisella
  • species: Allofrancisella guangzhouensis
  • full scientific name: Allofrancisella guangzhouensis (Qu et al. 2013) Qu et al. 2016

  • synonyms

    • @ref: 20215
    • synonym: Francisella guangzhouensis

@ref: 24473

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Thiotrichales

family: Francisellaceae

genus: Allofrancisella

species: Allofrancisella guangzhouensis

full scientific name: Allofrancisella guangzhouensis (Qu et al. 2013) Qu et al. 2016

strain designation: 08HL01032

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31018negativeovoid-shapedno
69480no93.036
69480negative99.881

colony morphology

  • @ref: 62214
  • incubation period: 1 day

Culture and growth conditions

culture medium

  • @ref: 24473
  • name: COLUMBIA-GLUCOSE-CYSTEINE-AGAR (DSMZ Medium 429c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/429c
  • composition: Name: COLUMBIA-GLUCOSE-CYSTEINE-AGAR (DSMZ Medium 429c) Composition: Horse blood 40.0 g/l Sheep blood 40.0 g/l Glucose 10.0 g/l Cysteine 1.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
24473positivegrowth30mesophilic
25033positiveoptimum25-28mesophilic
62214positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31018anaerobe
62214microaerophile

spore formation

@refspore formationconfidence
31018no
69481no100
69480no99.999

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
250334853esculin-hydrolysis
2503317632nitrate-reduction
2503316634raffinose-fermentation
2503327897tryptophan-energy source
2503317634D-glucose+fermentation
2503317992sucrose+fermentation
3101817234glucose+carbon source
3101817306maltose+carbon source
3101837684mannose+carbon source
3101817992sucrose+carbon source

metabolite production

  • @ref: 25033
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 25033
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
250336-phospho-beta-galactosidase-3.2.1.85
25033acid phosphatase+3.1.3.2
25033alanine arylamidase+3.4.11.2
25033alpha-arabinosidase-3.2.1.55
25033alpha-chymotrypsin-3.4.21.1
25033alpha-fucosidase-3.2.1.51
25033alpha-galactosidase-3.2.1.22
25033alpha-glucosidase-3.2.1.20
25033alpha-mannosidase-3.2.1.24
25033arginine arylamidase+
25033arginine dihydrolase-3.5.3.6
25033beta-glucosidase-3.2.1.21
25033beta-glucuronidase-3.2.1.31
25033catalase+1.11.1.6
25033cystine arylamidase-3.4.11.3
25033esterase (C 4)+
25033esterase lipase (C 8)+
25033glycin arylamidase-
25033histidine arylamidase-
25033leucine arylamidase-3.4.11.1
25033leucyl glycin arylamidase-3.4.11.1
25033lipase (C 14)-
25033naphthol-AS-BI-phosphohydrolase+
25033phenylalanine arylamidase-
25033proline-arylamidase-3.4.11.5
25033pyroglutamic acid arylamidase-
25033serine arylamidase-
25033trypsin-3.4.21.4
25033tyrosine arylamidase+
25033urease-3.5.1.5
25033valine arylamidase-
31018acid phosphatase+3.1.3.2
31018alkaline phosphatase+3.1.3.1
31018catalase+1.11.1.6
68382lipase (C 14)-
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
24473-+/-+/-+/--+/-----++/---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
24473water, air condition system (cooling tower)GuangzhouChinaCHNAsia
62214Water,air condition systemGuangzhou CDC,GuangzhouChinaCHNAsia2008-08-01

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Air conditioner
#Engineered#Industrial#Cooling tower
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_2711.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_68;96_1442;97_1720;98_2103;99_2711&stattab=map
  • Last taxonomy: Allofrancisella guangzhouensis
  • 16S sequence: CP010427
  • Sequence Identity:
  • Total samples: 166
  • soil counts: 17
  • aquatic counts: 144
  • animal counts: 5

Safety information

risk assessment

  • @ref: 24473
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessiondatabaselengthNCBI tax ID
2503316S rRNACP010427nuccore
24473Francisella guangzhouensis strain 08HL01032 16S ribosomal RNA gene, partial sequenceFJ591095ena1449594679

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Allofrancisella guangzhouensis 08HL01032GCA_000815225completencbi594679
66792Allofrancisella guangzhouensis 08HL010322718217988completeimg594679

GC content

@refGC-contentmethod
2503332.5high performance liquid chromatography (HPLC)
3101832.5
2447332.5sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno89.766yes
flagellatedno97.466yes
gram-positiveno97.283yes
anaerobicno98.229no
aerobicno61.632yes
halophileno66.237no
spore-formingno97.577yes
glucose-utilyes73.15no
thermophileno96.476yes
glucose-fermentno56.868no

External links

@ref: 24473

culture collection no.: DSM 102975, ATCC BAA 2361, CCUG 60119, NCTC 13503

straininfo link

  • @ref: 90992
  • straininfo: 396713

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23606480Francisella guangzhouensis sp. nov., isolated from air-conditioning systems.Qu PH, Chen SY, Scholz HC, Busse HJ, Gu Q, Kampfer P, Foster JT, Glaeser SP, Chen C, Yang ZCInt J Syst Evol Microbiol10.1099/ijs.0.049916-02013Air Conditioning, Bacterial Typing Techniques, China, Cysteine/metabolism, DNA, Bacterial/genetics, Fatty Acids/analysis, Francisella/*classification/genetics/isolation & purification, Genes, Bacterial, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, Polyamines/analysis, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis, *Water MicrobiologyGenetics
Genetics25792039Complete Genome Sequence of Francisella guangzhouensis Strain 08HL01032T, Isolated from Air-Conditioning Systems in China.Svensson D, Ohrman C, Backman S, Karlsson E, Nilsson E, Bystrom M, Larkeryd A, Myrtennas K, Stenberg P, Qu PH, Trygg J, Scholz HC, Forsman M, Sjodin AGenome Announc10.1128/genomeA.00024-152015Phylogeny
Phylogeny26413079Characterization of Francisella species isolated from the cooling water of an air conditioning system.Gu Q, Li X, Qu P, Hou S, Li J, Atwill ER, Chen SBraz J Microbiol10.1590/S1517-8382463201404652015*Air Conditioning, Bacterial Proteins/*genetics, Base Sequence, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, DNA-Directed RNA Polymerases/*genetics, Flavoproteins/*genetics, *Francisella/classification/genetics/isolation & purification, Molecular Sequence Data, Molecular Typing, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water MicrobiologyGenetics
Phylogeny27543089Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov.Qu PH, Li Y, Salam N, Chen SY, Liu L, Gu Q, Fang BZ, Xiao M, Li M, Chen C, Li WJInt J Syst Evol Microbiol10.1099/ijsem.0.0014372016Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Francisella, Gammaproteobacteria/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, *Water Microbiology, *Water SupplyTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmedID_cross_reference
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24473Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-102975Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102975)
25033P. H. L. Qu, Y.,Salam, N.,Chen, S. Y.,Liu, L.,Gu, Q.,Fang, B. Z.,Xiao, M.,Li, M.,Chen, C.,Li, W. J.Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov10.1099/ijsem.0.001437IJSEM 66: 4832-4838 201627543089
31018Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172877604127348
62214Curators of the CCUGhttps://www.ccug.se/strain?id=60119Culture Collection University of Gothenburg (CCUG) (CCUG 60119)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
90992Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID396713.1