Blastococcus capsensis | Type strain | DSM 46835, CECT 8876, BMG 804 | BacDiveID:132667

Strain identifier

BacDive ID: 132667

Type strain:

Species: Blastococcus capsensis

Culture col. no.: DSM 46835, CECT 8876, BMG 804

Strain history: <- M. Gtari, Tunesia; BMG 804 <- K. Hezbri, Tunesia

NCBI tax ID(s): 1564163 (species)

SI-ID 396603

DSM 46835

Section

Blastococcus capsensis DSM 46835 is a mesophilic, Gram-positive, coccus-shaped bacterium that has a bright orange pigmentation and was isolated from interior of a limestone rock in a wall of an archaeological Roman pool.

coccus-shaped
Gram-positive
pigmented
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Geodermatophilales*
	Family	*Geodermatophilaceae*
	Genus	*Blastococcus*
	Species	**Blastococcus capsensis**
	Full Scientific Name (LPSN)	Blastococcus capsensis Hezbri et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #24981]	Gram stain	positive

[Ref.: #24981]	Cell shape	coccus-shaped

[Ref.: #24981]	Motility	no

Colony color

bright orange

[Ref.: #24981]	Pigment production	yes
[Ref.: #24981]	Pigmentation color	bright orange

Multimedia content

Caption:

Medium 65 28°C

Intellectual property rights:

© Leibniz-Institut DSMZ

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24456]			growth	28 °C

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #24981]			growth	5.5-9.0

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #24981]		NaCl		growth	1	%
[Ref.: #24981]		NaCl		maximum	4	%

[Ref.: #24981]	Murein short key	A31
[Ref.: #24981]	Murein types	A1gamma m-Dpm-direct

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24981]		(S)-lactate ChEBI	-	oxidation
[Ref.: #24981]		2-oxobutanoate ChEBI	-	oxidation
[Ref.: #24981]		2-oxoglutarate ChEBI	+	oxidation
[Ref.: #24981]		3-hydroxybutyrate ChEBI	+	oxidation
[Ref.: #24981]		3-O-methyl-D-glucose ChEBI	+	oxidation
[Ref.: #24981]		4-hydroxyphenylacetic acid ChEBI	-	oxidation
[Ref.: #24981]		acetate ChEBI	+	oxidation
[Ref.: #24981]		acetoacetate ChEBI	+	oxidation
[Ref.: #24981]		alpha-lactose ChEBI	+	oxidation
[Ref.: #24981]		aztreonam ChEBI	+	oxidation
[Ref.: #24981]		bromosuccinate ChEBI	-	oxidation
[Ref.: #24981]		butyrate ChEBI	-	oxidation
[Ref.: #24981]		casein	-	degradation
[Ref.: #24981]		cellobiose ChEBI	+	oxidation
[Ref.: #24981]		D-arabitol ChEBI	-	oxidation
[Ref.: #24981]		D-aspartate ChEBI	-	oxidation
[Ref.: #24981]		D-fructose ChEBI	+	oxidation
[Ref.: #24981]		D-fructose 6-phosphate ChEBI	+	oxidation
[Ref.: #24981]		D-fucose ChEBI	+	oxidation
[Ref.: #24981]		D-galactose ChEBI	+	oxidation
[Ref.: #24981]		D-galacturonic acid ChEBI	+	oxidation
[Ref.: #24981]		D-glucarate ChEBI	-	oxidation
[Ref.: #24981]		D-gluconate ChEBI	+	oxidation
[Ref.: #24981]		D-glucose ChEBI	+	oxidation
[Ref.: #24981]		D-glucose 6-phosphate ChEBI	+	oxidation
[Ref.: #24981]		D-glucuronate ChEBI	+	oxidation
[Ref.: #24981]		D-malate ChEBI	+	oxidation
[Ref.: #24981]		D-mannitol ChEBI	-	oxidation
[Ref.: #24981]		D-mannose ChEBI	+	oxidation
[Ref.: #24981]		D-serine ChEBI	-	oxidation
[Ref.: #24981]		D-sorbitol ChEBI	+	oxidation
[Ref.: #24981]		D-trehalose ChEBI	+	oxidation
[Ref.: #24981]		dextrin ChEBI	-	oxidation
[Ref.: #24981]		esculin ChEBI	-	degradation
[Ref.: #24981]		fusidate ChEBI	-	oxidation
[Ref.: #24981]		galactarate ChEBI	-	oxidation
[Ref.: #24981]		gamma-aminobutyric acid ChEBI	-	oxidation
[Ref.: #24981]		gelatin ChEBI	-	degradation
[Ref.: #24981]		gelatin ChEBI	-	oxidation
[Ref.: #24981]		gentiobiose ChEBI	-	oxidation
[Ref.: #24981]		glucuronamide ChEBI	+	oxidation
[Ref.: #24981]		glycerol ChEBI	+	oxidation
[Ref.: #24981]		guanidinium chloride ChEBI	-	oxidation
[Ref.: #24981]		hypoxanthine ChEBI	-	degradation
[Ref.: #24981]		inosine ChEBI	-	oxidation
[Ref.: #24981]		L-alanine ChEBI	-	oxidation
[Ref.: #24981]		L-arginine ChEBI	-	oxidation
[Ref.: #24981]		L-aspartate ChEBI	+	oxidation
[Ref.: #24981]		L-fucose ChEBI	+	oxidation
[Ref.: #24981]		L-galactonic acid gamma-lactone ChEBI	-	oxidation
[Ref.: #24981]		L-glutamate ChEBI	+	oxidation
[Ref.: #24981]		L-histidine ChEBI	-	oxidation
[Ref.: #24981]		L-malate ChEBI	+	oxidation
[Ref.: #24981]		L-pyroglutamic acid ChEBI	+	oxidation
[Ref.: #24981]		L-serine ChEBI	-	oxidation
[Ref.: #24981]		lincomycin ChEBI	-	oxidation
[Ref.: #24981]		lithium chloride ChEBI	+	oxidation
[Ref.: #24981]		maltose ChEBI	+	oxidation
[Ref.: #24981]		melibiose ChEBI	+	oxidation
[Ref.: #24981]		methyl (R)-lactate ChEBI	+	oxidation
[Ref.: #24981]		methyl D-glucoside ChEBI	+	oxidation
[Ref.: #24981]		methyl pyruvate ChEBI	-	oxidation
[Ref.: #24981]		minocycline ChEBI	-	oxidation
[Ref.: #24981]		N-acetylglucosamine ChEBI	-	oxidation
[Ref.: #24981]		n-acetylneuraminate ChEBI	-	oxidation
[Ref.: #24981]		nalidixic acid ChEBI	+	oxidation
[Ref.: #24981]		niaproof ChEBI	-	oxidation
[Ref.: #24981]		nitrate ChEBI	-	reduction
[Ref.: #24981]		pectin ChEBI	+	oxidation
[Ref.: #24981]		propionate ChEBI	+	oxidation
[Ref.: #24981]		raffinose ChEBI	+	oxidation
[Ref.: #24981]		rifampicin ChEBI	-	oxidation
[Ref.: #24981]		salicin ChEBI	-	oxidation
[Ref.: #24981]		sodium bromate ChEBI	-	oxidation
[Ref.: #24981]		sodium formate ChEBI	-	oxidation
[Ref.: #24981]		sodium lactate ChEBI	-	oxidation
[Ref.: #24981]		stachyose ChEBI	+	oxidation
[Ref.: #24981]		starch ChEBI	-	degradation
[Ref.: #24981]		sucrose ChEBI	+	oxidation
[Ref.: #24981]		tetrazolium blue ChEBI	-	oxidation
[Ref.: #24981]		tetrazolium violet ChEBI	-	oxidation
[Ref.: #24981]		troleandomycin ChEBI	-	oxidation
[Ref.: #24981]		turanose ChEBI	+	oxidation
[Ref.: #24981]		tween 40 ChEBI	-	oxidation
[Ref.: #24981]		tyrosine ChEBI	-	degradation
[Ref.: #24981]		vancomycin ChEBI	-	oxidation
[Ref.: #24981]		xanthine ChEBI	-	degradation
		Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #24981]		alkaline phosphatase	+	3.1.3.1
[Ref.: #24981]		alpha-glucosidase	+	3.2.1.20
[Ref.: #24981]		catalase	-	1.11.1.6
[Ref.: #24981]		cytochrome oxidase	-	1.9.3.1
[Ref.: #24981]		esterase Lipase (C 8)	+
[Ref.: #24981]		leucine arylamidase	+	3.4.11.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24456]	Sample type/isolated from	interior of a limestone rock in a wall of an archaeological Roman pool
[Ref.: #24456]	Geographic location (country and/or sea, region)	place of the archaeological city Capsa, at present Gafsa (34° 25' N 008° 47' E, depth 285 m)
[Ref.: #24456]	Country	Tunisia
[Ref.: #24456]	Country ISO 3 Code	TUN
[Ref.: #24456]	Continent	Africa
[Ref.: #24456]	Geographic location	34.4167°/8.7833°

[Ref.: #24981]	Sample type/isolated from	dust collected from the interior of a limestone rock in a wall of an archaeological Roman pool
[Ref.: #24981]	Enrichment culture	Luedemann medium (DSMZ medium 877)
[Ref.: #24981]	Enrichment culture composition	supplemented with cycloheximide (100 Âµg ml-1)
[Ref.: #24981]	Enrichment culture duration	14 days
[Ref.: #24981]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Water reservoir (Aquarium/pool)
#Environmental	#Terrestrial	#Dust
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

Global distribution of 16S sequence LN626274 (>99% sequence identity) for Blastococcus from Microbeatlas

Aquatic Samples	440
Soil Samples	3945
Animal Samples	1429
Plant Samples	811
Total Samples	6625

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

1

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24456]	Blastococcus sp. BMG 804 partial 16S rRNA gene, strain BMG 804	LN626274	1329	ENA	1564163 tax ID

GC-content

73.7 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

Culture collection no.

DSM 46835, CECT 8876, BMG 804

SI-ID 396603

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Blastococcus xanthinilyticus sp. nov., isolated from monument.	Hezbri K, Nouioui I, Rohde M, Sproer C, Schumann P, Gtari M, Klenk HP, Montero-Calasanz MDC, Ghodhbane-Gtari F	Int J Syst Evol Microbiol	10.1099/ijsem.0.002646	2018	*
Phylogeny	Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus.	Hezbri K, Louati M, Nouioui I, Gtari M, Rohde M, Sproer C, Schumann P, Klenk HP, Ghodhbane-Gtari F, Montero-Calasanz MDC	Int J Syst Evol Microbiol	10.1099/ijsem.0.001443	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24456

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46835

#24981

K. L. Hezbri, Moussa,Nouioui, Imen,Gtari, Maher,Rohde, Manfred,Spröer, Cathrin,Schumann, Peter,Klenk, Hans-Peter,Ghodhbane-Gtari, Faten,Montero-Calasanz, Maria del Carmen: Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus. IJSEM 66: 4864 - 4872 2016 ( DOI 10.1099/ijsem.0.001443 , PubMed 27553620 )

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#90977

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396603.1 )

* These data were automatically processed and therefore are not curated

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