Strain identifier
BacDive ID: 132667
Type strain:
Species: Blastococcus capsensis
Strain history: <- M. Gtari, Tunesia; BMG 804 <- K. Hezbri, Tunesia
NCBI tax ID(s): 1564163 (species)
General
@ref: 24456
BacDive-ID: 132667
DSM-Number: 46835
keywords: 16S sequence, Bacteria, mesophilic, Gram-positive, coccus-shaped, pigmented
description: Blastococcus capsensis DSM 46835 is a mesophilic, Gram-positive, coccus-shaped bacterium that has a bright orange pigmentation and was isolated from interior of a limestone rock in a wall of an archaeological Roman pool.
NCBI tax id
- NCBI tax id: 1564163
- Matching level: species
strain history
- @ref: 24456
- history: <- M. Gtari, Tunesia; BMG 804 <- K. Hezbri, Tunesia
doi: 10.13145/bacdive132667.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Geodermatophilales
- family: Geodermatophilaceae
- genus: Blastococcus
- species: Blastococcus capsensis
- full scientific name: Blastococcus capsensis Hezbri et al. 2016
@ref: 24456
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Geodermatophilaceae
genus: Blastococcus
species: Blastococcus capsensis
full scientific name: Blastococcus capsensis Hezbri et al. 2016
type strain: yes
Morphology
cell morphology
- @ref: 24981
- gram stain: positive
- cell shape: coccus-shaped
- motility: no
colony morphology
- @ref: 24981
- colony color: bright orange
pigmentation
- @ref: 24981
- production: yes
- color: bright orange
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
24456 | https://www.dsmz.de/microorganisms/photos/DSM_46835.jpg | Medium 65 28°C | © Leibniz-Institut DSMZ |
66793 | EM_DSM_46835_1.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_46835_2.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_46835_3.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_46835_4.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_46835_5.jpg | electron microscopic image | © HZI/Manfred Rohde |
Culture and growth conditions
culture medium
- @ref: 24456
- name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
- growth: yes
- link: https://mediadive.dsmz.de/medium/65
- composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
culture temp
- @ref: 24456
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
culture pH
- @ref: 24981
- ability: positive
- type: growth
- pH: 5.5-9.0
- PH range: alkaliphile
Physiology and metabolism
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
24981 | NaCl | positive | growth | 1 % |
24981 | NaCl | maximum | 4 % |
murein
- @ref: 24981
- murein short key: A31
- type: A1gamma m-Dpm-direct
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
24981 | 16651 | (S)-lactate | - | oxidation |
24981 | 16763 | 2-oxobutanoate | - | oxidation |
24981 | 18101 | 4-hydroxyphenylacetic acid | - | oxidation |
24981 | 73706 | bromosuccinate | - | oxidation |
24981 | 17968 | butyrate | - | oxidation |
24981 | casein | - | degradation | |
24981 | 18333 | D-arabitol | - | oxidation |
24981 | 29990 | D-aspartate | - | oxidation |
24981 | 30612 | D-glucarate | - | oxidation |
24981 | 16899 | D-mannitol | - | oxidation |
24981 | 16523 | D-serine | - | oxidation |
24981 | 23652 | dextrin | - | oxidation |
24981 | 4853 | esculin | - | degradation |
24981 | 71321 | fusidate | - | oxidation |
24981 | 16537 | galactarate | - | oxidation |
24981 | 16865 | gamma-aminobutyric acid | - | oxidation |
24981 | 5291 | gelatin | - | degradation |
24981 | 5291 | gelatin | - | oxidation |
24981 | 28066 | gentiobiose | - | oxidation |
24981 | 32735 | guanidinium chloride | - | oxidation |
24981 | 17368 | hypoxanthine | - | degradation |
24981 | 17596 | inosine | - | oxidation |
24981 | 16977 | L-alanine | - | oxidation |
24981 | 16467 | L-arginine | - | oxidation |
24981 | 17464 | L-galactonic acid gamma-lactone | - | oxidation |
24981 | 15971 | L-histidine | - | oxidation |
24981 | 17115 | L-serine | - | oxidation |
24981 | 6472 | lincomycin | - | oxidation |
24981 | 51850 | methyl pyruvate | - | oxidation |
24981 | 50694 | minocycline | - | oxidation |
24981 | 506227 | N-acetylglucosamine | - | oxidation |
24981 | 35418 | n-acetylneuraminate | - | oxidation |
24981 | 75273 | niaproof | - | oxidation |
24981 | 17632 | nitrate | - | reduction |
24981 | 28077 | rifampicin | - | oxidation |
24981 | 17814 | salicin | - | oxidation |
24981 | 75229 | sodium bromate | - | oxidation |
24981 | 62965 | sodium formate | - | oxidation |
24981 | 75228 | sodium lactate | - | oxidation |
24981 | 28017 | starch | - | degradation |
24981 | 75198 | tetrazolium blue | - | oxidation |
24981 | 75193 | tetrazolium violet | - | oxidation |
24981 | 45735 | troleandomycin | - | oxidation |
24981 | 53423 | tween 40 | - | oxidation |
24981 | 18186 | tyrosine | - | degradation |
24981 | 28001 | vancomycin | - | oxidation |
24981 | 15318 | xanthine | - | degradation |
24981 | 15588 | D-malate | + | oxidation |
24981 | 15589 | L-malate | + | oxidation |
24981 | 30916 | 2-oxoglutarate | + | oxidation |
24981 | 37054 | 3-hydroxybutyrate | + | oxidation |
24981 | 73918 | 3-O-methyl-D-glucose | + | oxidation |
24981 | 30089 | acetate | + | oxidation |
24981 | 13705 | acetoacetate | + | oxidation |
24981 | 36219 | alpha-lactose | + | oxidation |
24981 | 161680 | aztreonam | + | oxidation |
24981 | 17057 | cellobiose | + | oxidation |
24981 | 15824 | D-fructose | + | oxidation |
24981 | 78697 | D-fructose 6-phosphate | + | oxidation |
24981 | 28847 | D-fucose | + | oxidation |
24981 | 12936 | D-galactose | + | oxidation |
24981 | 18024 | D-galacturonic acid | + | oxidation |
24981 | 8391 | D-gluconate | + | oxidation |
24981 | 17634 | D-glucose | + | oxidation |
24981 | 14314 | D-glucose 6-phosphate | + | oxidation |
24981 | 15748 | D-glucuronate | + | oxidation |
24981 | 17306 | maltose | + | oxidation |
24981 | 16024 | D-mannose | + | oxidation |
24981 | 28053 | melibiose | + | oxidation |
24981 | 16634 | raffinose | + | oxidation |
24981 | 17924 | D-sorbitol | + | oxidation |
24981 | 16551 | D-trehalose | + | oxidation |
24981 | 32323 | glucuronamide | + | oxidation |
24981 | 17754 | glycerol | + | oxidation |
24981 | 29991 | L-aspartate | + | oxidation |
24981 | 18287 | L-fucose | + | oxidation |
24981 | 29985 | L-glutamate | + | oxidation |
24981 | 18183 | L-pyroglutamic acid | + | oxidation |
24981 | 48607 | lithium chloride | + | oxidation |
24981 | 74611 | methyl (R)-lactate | + | oxidation |
24981 | 37657 | methyl D-glucoside | + | oxidation |
24981 | 100147 | nalidixic acid | + | oxidation |
24981 | 17309 | pectin | + | oxidation |
24981 | 17272 | propionate | + | oxidation |
24981 | 17164 | stachyose | + | oxidation |
24981 | 17992 | sucrose | + | oxidation |
24981 | 32528 | turanose | + | oxidation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
24981 | alkaline phosphatase | + | 3.1.3.1 |
24981 | alpha-glucosidase | + | 3.2.1.20 |
24981 | catalase | - | 1.11.1.6 |
24981 | cytochrome oxidase | - | 1.9.3.1 |
24981 | esterase Lipase (C 8) | + | |
24981 | leucine arylamidase | + | 3.4.11.1 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|---|---|
24456 | interior of a limestone rock in a wall of an archaeological Roman pool | place of the archaeological city Capsa, at present Gafsa (34° 25' N 008° 47' E, depth 285 m) | Tunisia | TUN | Africa | 34.4167 | 8.7833 | ||||
24981 | dust collected from the interior of a limestone rock in a wall of an archaeological Roman pool | Luedemann medium (DSMZ medium 877) | supplemented with cycloheximide (100 µg ml-1) | 14 days | 28 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Built environment | #Water reservoir (Aquarium/pool) |
#Environmental | #Terrestrial | #Dust |
#Environmental | #Terrestrial | #Geologic |
taxonmaps
- @ref: 69479
- File name: preview.99_3416.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_1096;97_2139;98_2620;99_3416&stattab=map
- Last taxonomy: Blastococcus
- 16S sequence: LN626274
- Sequence Identity:
- Total samples: 6625
- soil counts: 3945
- aquatic counts: 440
- animal counts: 1429
- plant counts: 811
Safety information
risk assessment
- @ref: 24456
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24456
- description: Blastococcus sp. BMG 804 partial 16S rRNA gene, strain BMG 804
- accession: LN626274
- length: 1329
- database: ena
- NCBI tax ID: 1564163
GC content
- @ref: 24456
- GC-content: 73.7
- method: high performance liquid chromatography (HPLC)
External links
@ref: 24456
culture collection no.: DSM 46835, CECT 8876, BMG 804
straininfo link
- @ref: 90977
- straininfo: 396603
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 27553620 | Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus. | Hezbri K, Louati M, Nouioui I, Gtari M, Rohde M, Sproer C, Schumann P, Klenk HP, Ghodhbane-Gtari F, Montero-Calasanz MDC | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001443 | 2016 | Actinomycetales/*classification/genetics/isolation & purification, Archaeology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Fresh Water/*microbiology, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tunisia, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 29458502 | Blastococcus xanthinilyticus sp. nov., isolated from monument. | Hezbri K, Nouioui I, Rohde M, Sproer C, Schumann P, Gtari M, Klenk HP, Montero-Calasanz MDC, Ghodhbane-Gtari F | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002646 | 2018 | Actinomycetales/*classification/genetics/isolation & purification, Architecture, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tunisia, Vitamin K 2/chemistry | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
24456 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-46835 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46835) | |||
24981 | K. L. Hezbri, Moussa,Nouioui, Imen,Gtari, Maher,Rohde, Manfred,Spröer, Cathrin,Schumann, Peter,Klenk, Hans-Peter,Ghodhbane-Gtari, Faten,Montero-Calasanz, Maria del Carmen | Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus | 10.1099/ijsem.0.001443 | IJSEM 66: 4864-4872 2016 | 27553620 | |
66793 | Mukherjee et al. | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 10.1038/nbt.3886 | 35: 676-683 2017 | 28604660 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
90977 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID396603.1 |