Name: Nesterenkonia aurantiaca CK5
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132446

Type strain:

Species: Nesterenkonia aurantiaca

Strain Designation: CK5

Culture col. no.: DSM 27373, JCM 19723, CIP 110620

Strain history: CIP <- 2013, A. Poli, CNR ICB, Pozzuoli, Italy: strain CK5

NCBI tax ID(s): 1436010 (species)

SI-ID 406146

DSM 27373

Special Collections

Section

Nesterenkonia aurantiaca CK5 is an aerobe, Gram-positive, coccus-shaped bacterium that forms circular colonies and was isolated from soil.

coccus-shaped
colony-forming
Gram-positive
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia aurantiaca**
	Full Scientific Name (LPSN)	Nesterenkonia aurantiaca Finore et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43734]	Gram stain	positive
[Ref.: #116553]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %91.925

[Ref.: #43734]	Cell length	1.5 µm

[Ref.: #43734]	Cell shape	coccus-shaped
[Ref.: #116553]	Cell shape	coccus-shaped

[Ref.: #43734]	Motility	no
[Ref.: #116553]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94.5

[Ref.: #43734]	Colony size	1 mm
[Ref.: #43734]	Colony color	Orange
[Ref.: #43734]	Colony shape	circular
[Ref.: #43734]	Incubation period	2 days
[Ref.: #43734]	Cultivation medium used	Solid medium A

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43734]

Culture medium

Solid medium A

[Ref.: #43734]

Culture medium growth

[Ref.: #24235]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #24235]

Culture medium growth

[Ref.: #24235]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24235]

Culture medium composition

expand / minimize

[Ref.: #24235]

Culture medium

HORIKOSHI-1 MEDIUM (DSMZ Medium 1081)

[Ref.: #24235]

Culture medium growth

[Ref.: #24235]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24235]

Culture medium composition

expand / minimize

[Ref.: #116553]

Culture medium

CIP Medium 13

[Ref.: #116553]

Culture medium growth

[Ref.: #116553]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #24235]	growth	25 °C
[Ref.: #43734]	growth	4-30 °C
[Ref.: #43734]	optimum	25 °C
[Ref.: #67770]	growth	25 °C

	pH	Kind of pH		pH
[Ref.: #43734]			optimum	9
[Ref.: #43734]			growth	6.5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43734]

Oxygen tolerance

aerobe

[Ref.: #43734]	Type of spore	endospore
[Ref.: #43734]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

[Ref.: #43734]

Halophily / tolerance level

slightly halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43734]		NaCl		growth	0.003-20	%(w/v)
[Ref.: #43734]		NaCl		optimum	2	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-7, MK-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43734]	acetate ChEBI	+	assimilation
[Ref.: #43734]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43734]	casein	-	hydrolysis
[Ref.: #43734]	citrate ChEBI	+	assimilation
[Ref.: #43734]	D-fructose ChEBI	-	builds acid from
[Ref.: #43734]	D-fructose ChEBI	+	carbon source
[Ref.: #43734]	D-fructose ChEBI	+	energy source
[Ref.: #43734]	D-glucose ChEBI	-	builds acid from
[Ref.: #43734]	D-glucose ChEBI	+	carbon source
[Ref.: #43734]	D-glucose ChEBI	+	energy source
[Ref.: #43734]	D-mannose ChEBI	-	assimilation
[Ref.: #43734]	D-xylose ChEBI	-	builds acid from
[Ref.: #43734]	D-xylose ChEBI	+	carbon source
[Ref.: #43734]	D-xylose ChEBI	+	energy source
[Ref.: #43734]	formate ChEBI	-	assimilation
[Ref.: #43734]	fumarate ChEBI	-	assimilation
[Ref.: #43734]	galactose ChEBI	-	builds acid from
[Ref.: #43734]	galactose ChEBI	+	carbon source
[Ref.: #43734]	galactose ChEBI	+	energy source
[Ref.: #43734]	gelatin ChEBI	-	hydrolysis
[Ref.: #43734]	gluconate ChEBI	-	assimilation
[Ref.: #43734]	hippurate ChEBI	-	hydrolysis
[Ref.: #43734]	L-alanine ChEBI	+	energy source
[Ref.: #43734]	L-alanine ChEBI	+	nitrogen source
[Ref.: #43734]	L-arabinose ChEBI	-	assimilation
[Ref.: #43734]	L-cysteine ChEBI	-	energy source
[Ref.: #43734]	L-cysteine ChEBI	-	nitrogen source
[Ref.: #43734]	L-histidine ChEBI	-	energy source
[Ref.: #43734]	L-histidine ChEBI	-	nitrogen source
[Ref.: #43734]	L-isoleucine ChEBI	-	energy source
[Ref.: #43734]	L-isoleucine ChEBI	-	nitrogen source
[Ref.: #43734]	L-lysine ChEBI	-	energy source
[Ref.: #43734]	L-lysine ChEBI	-	nitrogen source
[Ref.: #43734]	L-methionine ChEBI	-	energy source
[Ref.: #43734]	L-methionine ChEBI	-	nitrogen source
[Ref.: #43734]	L-serine ChEBI	-	energy source
[Ref.: #43734]	L-serine ChEBI	-	nitrogen source
[Ref.: #43734]	L-valine ChEBI	-	energy source
[Ref.: #43734]	L-valine ChEBI	-	nitrogen source
[Ref.: #43734]	lactose ChEBI	-	builds acid from
[Ref.: #43734]	lactose ChEBI	+	carbon source
[Ref.: #43734]	lactose ChEBI	+	energy source
[Ref.: #43734]	malonate ChEBI	+	assimilation
[Ref.: #43734]	nitrate ChEBI	-	reduction
[Ref.: #43734]	nitrite ChEBI	-	reduction
[Ref.: #43734]	pectin ChEBI	+	hydrolysis
[Ref.: #43734]	phenylalanine ChEBI	-	hydrolysis
[Ref.: #43734]	propionate ChEBI	+	assimilation
[Ref.: #43734]	raffinose ChEBI	-	assimilation
[Ref.: #43734]	starch ChEBI	-	hydrolysis
[Ref.: #43734]	succinate ChEBI	+	assimilation
[Ref.: #43734]	sucrose ChEBI	-	builds acid from
[Ref.: #43734]	sucrose ChEBI	+	carbon source
[Ref.: #43734]	sucrose ChEBI	+	energy source
[Ref.: #43734]	trehalose ChEBI	-	builds acid from
[Ref.: #43734]	trehalose ChEBI	+	carbon source
[Ref.: #43734]	trehalose ChEBI	+	energy source
[Ref.: #43734]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43734]	urea ChEBI	-	hydrolysis
[Ref.: #43734]	xylan ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #43734]	bacitracin ChEBI	yes	10	Unit (disc)
[Ref.: #43734]	chloramphenicol ChEBI	yes	10	µg (disc)
[Ref.: #43734]	fusidate ChEBI	yes	10	µg (disc)
[Ref.: #43734]	lincomycin ChEBI	yes	15	µg (disc)
[Ref.: #43734]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43734]	nystatin ChEBI	yes	100	Unit (disc)
[Ref.: #43734]	penicillin ChEBI	yes	2	Unit (disc)
[Ref.: #43734]	streptomycin ChEBI	yes	25	µg (disc)
[Ref.: #43734]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #43734]	vancomycin ChEBI	yes	30	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #43734]		acetoin ChEBI	no
[Ref.: #43734]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #43734]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #43734]	catalase	+	1.11.1.6
[Ref.: #43734]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43734]	lipase (C 14)	+
[Ref.: #43734]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24235]	Sample type/isolated from	soil
[Ref.: #24235]	Geographic location (country and/or sea, region)	Cape King (73° 05' S 166° 40'E)
[Ref.: #24235]	Country	Antarctica
[Ref.: #24235]	Country ISO 3 Code	ATA
[Ref.: #24235]	Continent	Australia and Oceania
[Ref.: #24235]	Geographic location	-73.0833°/166.6670°

[Ref.: #43734]	Sample type/isolated from	Soil samples
[Ref.: #43734]	Geographic location (country and/or sea, region)	Cape King
[Ref.: #43734]	Country	Antarctica
[Ref.: #43734]	Country ISO 3 Code	ATA
[Ref.: #43734]	Continent	Australia and Oceania
[Ref.: #43734]	Geographic location	-73.0830°/166.6660°

[Ref.: #67770]	Sample type/isolated from	Soil from Cape King
[Ref.: #67770]	Country	Antarctica
[Ref.: #67770]	Country ISO 3 Code	ATA
[Ref.: #67770]	Continent	Antarctica

[Ref.: #116553]	Sample type/isolated from	Environment, Soil, lake
[Ref.: #116553]	Geographic location (country and/or sea, region)	Cape king lake
[Ref.: #116553]	Country	Antarctica
[Ref.: #116553]	Country ISO 3 Code	ATA
[Ref.: #116553]	Continent	Antarctica
[Ref.: #116553]	Isolation date	2006-06-08
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HG795012 (>99% sequence identity) for Nesterenkonia from Microbeatlas

Aquatic Samples	600
Soil Samples	791
Animal Samples	2130
Plant Samples	449
Total Samples	3970

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24235]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116553]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24235]	Nesterenkonia sp. DSM 27373 partial 16S rRNA gene, strain DSM 27373, isolate CK5	HG795012	1481	GenBank ENA	1436010 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nesterenkonia aurantiaca DSM 27373	GCA_004364585	scaffold	GenBank ENA	1436010 tax ID
[Ref.: #66792]	Nesterenkonia aurantiaca strain DSM 27373	1436010.5	wgs	PATRIC	1436010 tax ID	*
[Ref.: #66792]	Nesterenkonia aurantiaca DSM 27373	2802428805	draft	JGI IMG/M	1436010 tax ID	*

[Ref.: #43734]	GC-content	64.8 mol%
[Ref.: #24235]	GC-content	64.8 mol%	fluorimetric
[Ref.: #67770]	GC-content	64.8 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nesterenkonia aurantiaca DSM 27373 NCBI: GCA_004364585.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nesterenkonia aurantiaca strain DSM 27373 PATRIC: 1436010.5
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	91.925	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	98.667	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	73.363	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	89.504	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	91	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	94.5	yes

Availability in culture collections External links

[Ref.: #24235]

Culture collection no.

DSM 27373, JCM 19723, CIP 110620

[Ref.: #90784]

SI-ID 406146

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica.	Finore I, Orlando P, Di Donato P, Leone L, Nicolaus B, Poli A	Int J Syst Evol Microbiol	10.1099/ijsem.0.000917	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24235

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27373

#43734

Ilaria Finore, Pierangelo Orlando, Paola Di Donato, Luigi Leone, Barbara Nicolaus, Annarita Poli: Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica. IJSEM 66: 1554 - 1560 2016 ( DOI 10.1099/ijsem.0.000917 , PubMed 26813578 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90784

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406146.1 )

#116553 Collection of Institut Pasteur ; Curators of the CIP; CIP 110620
* These data were automatically processed and therefore are not curated

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Nesterenkonia aurantiaca DSM 27373

Nesterenkonia aurantiaca strain DSM 27373

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