Name: Methanosarcina flavescens E03.2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132429

Type strain:

Species: Methanosarcina flavescens

Strain Designation: E03.2

Culture col. no.: DSM 100822, JCM 30921

Strain history: M. Rother; Tech. Univ. Dresden, Germany; E03.2.

NCBI tax ID(s): 1715806 (species)

SI-ID 405730

DSM 100822

Special Collections

Section

Methanosarcina flavescens E03.2 is an obligate anaerobe, non-halophilic, autotroph archaeon that forms irregular colonies and was isolated from full-scale commercial biogas plant fed with maize silage, cattle manure and dry poultry feces.

coccus-shaped
colony-forming
autotroph
non-halophilic
obligate anaerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanosarcinales*
	Family	*Methanosarcinaceae*
	Genus	*Methanosarcina*
	Species	**Methanosarcina flavescens**
	Full Scientific Name (LPSN)	Methanosarcina flavescens Kern et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43730]	Cell length	1-2 µm

[Ref.: #43730]	Cell shape	coccus-shaped

[Ref.: #43730]	Motility	no

[Ref.: #43730]	Colony color	Dark Yellow
[Ref.: #43730]	Colony shape	irregular
[Ref.: #43730]	Cultivation medium used	BL medium agar plates

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43730]

Culture medium

BL medium agar plates

[Ref.: #43730]

Culture medium growth

[Ref.: #24218]

Culture medium

METHANOSARCINA MEDIUM (DSMZ Medium 120)

[Ref.: #24218]

Culture medium growth

[Ref.: #24218]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24218]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24218]	growth	45 °C
[Ref.: #43730]	growth	20-55 °C
[Ref.: #43730]	optimum	45 °C
[Ref.: #67770]	growth	45 °C

	pH	Kind of pH		pH
[Ref.: #43730]			optimum	7
[Ref.: #43730]			growth	5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43730]

Oxygen tolerance

obligate anaerobe

[Ref.: #43730]

Nutrition type

autotroph

[Ref.: #43730]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43730]		NaCl		optimum	0.05	M

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43730]	acetate ChEBI	+	growth
[Ref.: #43730]	carbon dioxide ChEBI	+	growth
[Ref.: #43730]	dihydrogen ChEBI	+	growth
[Ref.: #43730]	dimethylamine ChEBI	+	growth
[Ref.: #43730]	methylamine ChEBI	+	growth
[Ref.: #43730]	trimethylamine ChEBI	+	growth

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24218]	Sample type/isolated from	full-scale commercial biogas plant fed with maize silage, cattle manure and dry poultry feces
[Ref.: #24218]	Country	Germany
[Ref.: #24218]	Country ISO 3 Code	DEU
[Ref.: #24218]	Continent	Europe

[Ref.: #43730]	Sample type/isolated from	a biogas sludge sample from a full-scale biogas plant
[Ref.: #43730]	Geographic location (country and/or sea, region)	Dresden
[Ref.: #43730]	Country	Germany
[Ref.: #43730]	Country ISO 3 Code	DEU
[Ref.: #43730]	Continent	Europe
[Ref.: #43730]	Isolation date	2013-03

[Ref.: #67770]	Sample type/isolated from	Biogas sludge sample
[Ref.: #67770]	Geographic location (country and/or sea, region)	Dresden
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Birds	-
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Industrial	#Plant (Factory)
#Environmental	#Terrestrial	#Mud (Sludge)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24218]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24218]	Methanosarcina flavescens strain E03.2 16S ribosomal RNA gene, partial sequence	KT828541	1409	GenBank ENA	1715806 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Methanosarcina flavescens E03.2	GCA_001304615	complete	GenBank ENA	1715806 tax ID
[Ref.: #66792]	Methanosarcina flavescens E03.2	2651869834	draft	JGI IMG/M	1715806 tax ID	*

[Ref.: #24218]	GC-content	41.3 mol%	sequence analysis
[Ref.: #67770]	GC-content	41.3 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methanosarcina flavescens E03.2 NCBI: GCA_001304615.2
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	84	no

Availability in culture collections External links

[Ref.: #24218]

Culture collection no.

DSM 100822, JCM 30921

[Ref.: #90768]

SI-ID 405730

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester.	Kern T, Fischer MA, Deppenmeier U, Schmitz RA, Rother M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000894	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24218

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100822

#43730

Tobias Kern, Martin A. Fischer, Uwe Deppenmeier, Ruth A. Schmitz, Michael Rother: Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester. IJSEM 66: 1533 - 1538 2016 ( DOI 10.1099/ijsem.0.000894 , PubMed 26763977 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90768

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405730.1 )

* These data were automatically processed and therefore are not curated

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