Methanosarcina flavescens (DSM 100822, JCM 30921, E03.2)

Name: Methanosarcina flavescens (DSM 100822, JCM 30921, E03.2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132429

Type strain

Strain Designation E03.2

Culture col. no. DSM 100822 JCM 30921 E03.2

NCBI tax ID(s) 1715806

Special Collections DSMZ JCM Literature strains

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Methanosarcina flavescens E03.2 is an obligate anaerobe, non-halophilic, autotroph prokaryote that forms irregular colonies and was isolated from full-scale commercial biogas plant fed with maize silage, cattle manure and dry poultry feces.

coccus-shaped colony-forming obligate anaerobe non-halophilic autotroph genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405730

JCM 30921,DSM 100822

@ref 20215

Last LPSN update 2025-12-16 10:01:30

Domain Methanobacteriati

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanosarcina

Species Methanosarcina flavescens

Full scientific name Methanosarcina flavescens Kern et al. 2016

Morphology

Cell morphology

@ref	Cell length	Cell shape	Motility
43730	1-2 µm	coccus-shaped
125439

Colony morphology

@ref	Colony color	Colony shape	Medium used
43730	Dark Yellow	irregular	BL medium agar plates

Culture and growth conditions

Culture medium

@ref	Name	Growth
43730	BL medium agar plates
24218	METHANOSARCINA MEDIUM (DSMZ Medium 120)

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24218	positive	growth	45	thermophilic
43730	positive	growth	20-55
43730	positive	optimum	45	thermophilic
67770	positive	growth	45	thermophilic

Culture pH

@ref	Ability	Type	PH
43730	positive	optimum	7
43730	positive	growth	5-8.5

Physiology and metabolism

Oxygen tolerance

43730

Oxygen toleranceobligate anaerobe

Nutrition type

43730

Typeautotroph

Spore formation

@ref	Spore formation	Confidence
125439		92.7

Halophily

@ref	Halophily level	Salt	Growth	Tested relation	Concentration
43730	non-halophilic	NaCl	positive	optimum	0.05 M

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43730	30089 ChEBI	acetate	+	growth
43730	16526 ChEBI	carbon dioxide	+	growth
43730	18276 ChEBI	dihydrogen	+	growth
43730	17170 ChEBI	dimethylamine	+	growth
43730	16830 ChEBI	methylamine	+	growth
43730	18139 ChEBI	trimethylamine	+	growth

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host	#Birds
#Host	#Mammals
#Host Body Product	#Gastrointestinal tract
#Engineered	#Biodegradation
#Engineered	#Industrial
#Environmental	#Terrestrial

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
24218	full-scale commercial biogas plant fed with maize silage, cattle manure and dry poultry feces	Germany	DEU	Europe
43730	a biogas sludge sample from a full-scale biogas plant	Germany	DEU	Europe
67770	Biogas sludge sample	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24218	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM130461v2 assembly for Methanosarcina flavescens E03.2	complete	GCA_001304615	1715806.8	2651869834	1715806	95.03

Genome browser: GCA_001304615

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24218	Methanosarcina flavescens strain E03.2 16S ribosomal RNA gene, partial sequence	KT828541	1409		1715806

GC content

@ref	GC-content (mol%)	Method
24218	41.3	sequence analysis
67770	41.3	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanosarcina flavescens E03.2
IMG: 2651869834

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	92.70	no
125439	motility	BacteriaNetⓘ	no	79.80	no
125439	gram_stain	BacteriaNetⓘ	negative	98.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	71.90	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester.	Kern T, Fischer MA, Deppenmeier U, Schmitz RA, Rother M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000894	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24218	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100822
#43730	Tobias Kern, Martin A. Fischer, Uwe Deppenmeier, Ruth A. Schmitz, Michael Rother: Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester. IJSEM 66: 1533 - 1538 2016 ( DOI 10.1099/ijsem.0.000894 , PubMed 26763977 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanosarcina flavescens (DSM 100822, JCM 30921, E03.2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001304615

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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