Name: Nocardiopsis ansamitocini DSM 103990
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132343

Type strain:

Species: Nocardiopsis ansamitocini

Culture col. no.: DSM 103990, CGMCC 9969, KCTC 39605, EGI 80425

Strain history: <- Y.-G. Zhang, Xinjiang Institute of Ecology and Geography, CAS; EGI 80425

NCBI tax ID(s): 1670832 (species)

SI-ID 406421

DSM 103990

Special Collections

Section

When using BacDive for research please cite our paper

Nocardiopsis ansamitocini DSM 103990 is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and was isolated from desert soil.

Gram-positive
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Nocardiopsidaceae*
	Genus	*Nocardiopsis*
	Species	**Nocardiopsis ansamitocini**
	Full Scientific Name (LPSN)	Nocardiopsis ansamitocini Zhang et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #44063]	Gram stain	positive

[Ref.: #44063]	Multicellular complex forming ability	yes
[Ref.: #44063]	Multicellular complex name	substrate mycelium
[Ref.: #44063]	Multicellular complex color	white-orange-yellow
[Ref.: #44063]	Multicellular complex further description	non-fragmented

[Ref.: #44063]	Multicellular complex forming ability	yes
[Ref.: #44063]	Multicellular complex name	aerial mycelium
[Ref.: #44063]	Multicellular complex color	white
[Ref.: #44063]	Medium Name (multicellularity)	ISP2

[Ref.: #44063]	Multicellular complex forming ability	yes
[Ref.: #44063]	Multicellular complex name	aerial mycelium
[Ref.: #44063]	Multicellular complex color	white
[Ref.: #44063]	Medium Name (multicellularity)	ISP3

[Ref.: #44063]	Multicellular complex forming ability	yes
[Ref.: #44063]	Multicellular complex name	aerial mycelium
[Ref.: #44063]	Multicellular complex color	white
[Ref.: #44063]	Medium Name (multicellularity)	nutrient agar

[Ref.: #44063]	Multicellular complex forming ability	yes
[Ref.: #44063]	Multicellular complex name	aerial mycelium
[Ref.: #44063]	Multicellular complex color	white
[Ref.: #44063]	Medium Name (multicellularity)	potato-dextrose agar

[Ref.: #44063]	Multicellular complex forming ability	no
[Ref.: #44063]	Multicellular complex name	aerial mycelium
[Ref.: #44063]	Multicellular complex color	white
[Ref.: #44063]	Medium Name (multicellularity)	ISP4

[Ref.: #44063]	Multicellular complex forming ability	no
[Ref.: #44063]	Multicellular complex name	aerial mycelium
[Ref.: #44063]	Multicellular complex color	white
[Ref.: #44063]	Medium Name (multicellularity)	ISP5

[Ref.: #44063]	Multicellular complex forming ability	no
[Ref.: #44063]	Multicellular complex name	aerial mycelium
[Ref.: #44063]	Multicellular complex color	white
[Ref.: #44063]	Medium Name (multicellularity)	Czapek's agar

[Ref.: #44063]	Multicellular complex forming ability	no
[Ref.: #44063]	Multicellular complex name	aerial mycelium
[Ref.: #44063]	Multicellular complex color	white
[Ref.: #44063]	Medium Name (multicellularity)	Gauze No. 1 agar

Multimedia content

[Ref.: #24132]

Caption:

Medium 553 28°C

[Ref.: #24132]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #44063]

Culture medium

Czapek's agar

[Ref.: #44063]

Culture medium growth

[Ref.: #44063]

Culture medium

Gauze No. 1 agar

[Ref.: #44063]

Culture medium growth

[Ref.: #44063]

Culture medium

ISP2

[Ref.: #44063]

Culture medium growth

[Ref.: #44063]

Culture medium

ISP3

[Ref.: #44063]

Culture medium growth

[Ref.: #44063]

Culture medium

ISP4

[Ref.: #44063]

Culture medium growth

[Ref.: #44063]

Culture medium

ISP5

[Ref.: #44063]

Culture medium growth

[Ref.: #44063]

Culture medium

Nutrient agar (NA)

[Ref.: #44063]

Culture medium growth

[Ref.: #44063]

Culture medium

potato-dextrose agar

[Ref.: #44063]

Culture medium growth

[Ref.: #24132]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #24132]

Culture medium growth

[Ref.: #24132]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24132]

Culture medium composition

expand / minimize

[Ref.: #24132]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #24132]

Culture medium growth

[Ref.: #24132]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #24132]	growth	28 °C
[Ref.: #44063]	growth	15-50 °C
[Ref.: #44063]	optimum	30 °C

	pH	Kind of pH		pH
[Ref.: #44063]			optimum	8.0-9.0
[Ref.: #44063]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44063]

Oxygen tolerance

aerobe

[Ref.: #44063]	Spore description	rod-like spore chains
[Ref.: #44063]	Type of spore	spore
[Ref.: #44063]	Ability of spore formation	yes

[Ref.: #44063]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #44063]		NaCl		growth	0-12	%(w/v)
[Ref.: #44063]		NaCl		optimum	0	%(w/v)

[Ref.: #44063]

Observation

production of ansamitocin P-3

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44063]	casein	-	hydrolysis
[Ref.: #44063]	cellulose ChEBI	+	hydrolysis
[Ref.: #44063]	D-arabinose ChEBI	+	carbon source
[Ref.: #44063]	D-fructose ChEBI	+	carbon source
[Ref.: #44063]	D-galactose ChEBI	+	carbon source
[Ref.: #44063]	D-glucose ChEBI	+	carbon source
[Ref.: #44063]	D-mannose ChEBI	+	carbon source
[Ref.: #44063]	D-ribose ChEBI	+	carbon source
[Ref.: #44063]	D-sorbitol ChEBI	+	carbon source
[Ref.: #44063]	D-xylose ChEBI	+	carbon source
[Ref.: #44063]	dl-alanine ChEBI	+	nitrogen source
[Ref.: #44063]	glycerol ChEBI	-	carbon source
[Ref.: #44063]	L-arginine ChEBI	+	nitrogen source
[Ref.: #44063]	L-asparagine ChEBI	-	nitrogen source
[Ref.: #44063]	L-aspartate ChEBI	-	nitrogen source
[Ref.: #44063]	L-cysteine ChEBI	-	nitrogen source
[Ref.: #44063]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #44063]	L-histidine ChEBI	-	nitrogen source
[Ref.: #44063]	L-isoleucine ChEBI	+	nitrogen source
[Ref.: #44063]	L-lysine ChEBI	+	nitrogen source
[Ref.: #44063]	L-methionine ChEBI	+	nitrogen source
[Ref.: #44063]	L-phenylalanine ChEBI	+	nitrogen source
[Ref.: #44063]	L-rhamnose ChEBI	+	carbon source
[Ref.: #44063]	L-serine ChEBI	+	nitrogen source
[Ref.: #44063]	L-sorbose ChEBI	+	carbon source
[Ref.: #44063]	L-threonine ChEBI	+	nitrogen source
[Ref.: #44063]	L-tryptophan ChEBI	-	nitrogen source
[Ref.: #44063]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #44063]	L-valine ChEBI	+	nitrogen source
[Ref.: #44063]	lactose ChEBI	+	carbon source
[Ref.: #44063]	maltose ChEBI	+	carbon source
[Ref.: #44063]	myo-inositol ChEBI	+	carbon source
[Ref.: #44063]	nitrate ChEBI	-	reduction
[Ref.: #44063]	propionate ChEBI	+	carbon source
[Ref.: #44063]	raffinose ChEBI	+	carbon source
[Ref.: #44063]	sodium citrate ChEBI	+	carbon source
[Ref.: #44063]	starch ChEBI	-	hydrolysis
[Ref.: #44063]	sucrose ChEBI	-	carbon source
[Ref.: #44063]	trehalose ChEBI	+	carbon source
[Ref.: #44063]	tween 20 ChEBI	+	hydrolysis
[Ref.: #44063]	tween 40 ChEBI	+	hydrolysis
[Ref.: #44063]	tween 60 ChEBI	-	hydrolysis
[Ref.: #44063]	tween 80 ChEBI	+	hydrolysis
[Ref.: #44063]	xylitol ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #44063]		hydrogen sulfide ChEBI	no

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44063]

Fatty acid	Percentage
C17:0 anteiso	25.66
C16:0 iso	20.66
C18:1ω9c	20.13
C17:0 iso	6.68
10-Methyl C18:0-TBSA	6.53
C18:0	3.98
C16:0	2.39
C17:1ω8c	2.14
10-Methyl C17:0	1.97
C15:0 anteiso	1.81
C18:0 iso	1.68
C16:1ω7c / C16:1ω6c	1.44
C15:0 iso	1.03
C19:0 anteiso	0.65
C19:0 iso	0.61
iso-C17:1ω9c / 10-methyl-C16:0	0.52
unkoωn 18.846/C19:1ω6c or C19:1ω	0.41
C14:0 iso	0.4
C17:0 2OH	0.38
iso-C17:1-I / anteiso-C17:1-B	0.31
C17:0	0.31
C14:0	0.17
C18:1ω7c / C18:1ω6c	0.15

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24132]	Sample type/isolated from	desert soil
[Ref.: #24132]	Geographic location (country and/or sea, region)	Xinjiang Province, Karamay
[Ref.: #24132]	Country	China
[Ref.: #24132]	Country ISO 3 Code	CHN
[Ref.: #24132]	Continent	Asia

[Ref.: #44063]	Sample type/isolated from	saline-alkali soil sample
[Ref.: #44063]	Geographic location (country and/or sea, region)	Karamay, Xinjiang Province
[Ref.: #44063]	Country	China
[Ref.: #44063]	Country ISO 3 Code	CHN
[Ref.: #44063]	Continent	Asia
[Ref.: #44063]	Enrichment culture	ISP2
[Ref.: #44063]	Enrichment culture composition	adjusted to pH 10 with NaOH
[Ref.: #44063]	Enrichment culture duration	28 days
[Ref.: #44063]	Enrichment culture temperature	30 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Desert
#Condition	#Alkaline	-
#Condition	#Saline	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24132]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24132]	Nocardiopsis ansamitocini strain EGI 80425 16S ribosomal RNA gene, partial sequence	KR605109	1524	GenBank ENA	1670832 tax ID

[Ref.: #24132]

GC-content

70.2 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24132]

Culture collection no.

DSM 103990, CGMCC 9969, KCTC 39605, EGI 80425

[Ref.: #90689]

SI-ID 406421

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis.	Zhang YG, Liu Q, Wang HF, Park DJ, Guo JW, Kim CJ, Zhang YM, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000703	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24132

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103990

#44063

Yong-Guang Zhang, Qing Liu, Hong-Fei Wang, Dong-Jin Park, Jian-Wei Guo, Chang-Jin Kim, Yuan-Ming Zhang and Wen-Jun Li: Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis. IJSEM 66: 230 - 235 2016 ( DOI 10.1099/ijsem.0.000703 , PubMed 26486850 )

#90689

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406421.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Nocardiopsis ansamitocini in external resources:
SILVA
BRENDA
PANGAEA
GBIF