Strain identifier
BacDive ID: 132343
Type strain:
Species: Nocardiopsis ansamitocini
Strain history: <- Y.-G. Zhang, Xinjiang Institute of Ecology and Geography, CAS; EGI 80425
NCBI tax ID(s): 1670832 (species)
General
@ref: 24132
BacDive-ID: 132343
DSM-Number: 103990
keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive
description: Nocardiopsis ansamitocini DSM 103990 is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and was isolated from desert soil.
NCBI tax id
- NCBI tax id: 1670832
- Matching level: species
strain history
- @ref: 24132
- history: <- Y.-G. Zhang, Xinjiang Institute of Ecology and Geography, CAS; EGI 80425
doi: 10.13145/bacdive132343.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Streptosporangiales
- family: Nocardiopsidaceae
- genus: Nocardiopsis
- species: Nocardiopsis ansamitocini
- full scientific name: Nocardiopsis ansamitocini Zhang et al. 2016
@ref: 24132
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Nocardiopsaceae
genus: Nocardiopsis
species: Nocardiopsis ansamitocini
full scientific name: Nocardiopsis ansamitocini Zhang et al. 2016
type strain: yes
Morphology
cell morphology
- @ref: 44063
- gram stain: positive
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | further description | medium name |
---|---|---|---|---|---|
44063 | yes | substrate mycelium | white-orange-yellow | non-fragmented | |
44063 | yes | aerial mycelium | white | ISP2 | |
44063 | yes | aerial mycelium | white | ISP3 | |
44063 | yes | aerial mycelium | white | nutrient agar | |
44063 | yes | aerial mycelium | white | potato-dextrose agar | |
44063 | no | aerial mycelium | white | ISP4 | |
44063 | no | aerial mycelium | white | ISP5 | |
44063 | no | aerial mycelium | white | Czapek's agar | |
44063 | no | aerial mycelium | white | Gauze No. 1 agar |
multimedia
- @ref: 24132
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_103990.jpg
- caption: Medium 553 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
44063 | Czapek's agar | yes | ||
44063 | Gauze No. 1 agar | yes | ||
44063 | ISP2 | yes | ||
44063 | ISP3 | yes | ||
44063 | ISP4 | yes | ||
44063 | ISP5 | yes | ||
44063 | Nutrient agar (NA) | yes | ||
44063 | potato-dextrose agar | yes | ||
24132 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
44063 | positive | growth | 15-50 | |
44063 | positive | optimum | 30 | mesophilic |
24132 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
44063 | positive | growth | 6.0-10.0 | alkaliphile |
44063 | positive | optimum | 8.0-9.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 44063
- oxygen tolerance: aerobe
spore formation
- @ref: 44063
- spore description: rod-like spore chains
- type of spore: spore
- spore formation: yes
halophily
@ref | salt | growth | tested relation | concentration | halophily level |
---|---|---|---|---|---|
44063 | NaCl | positive | growth | 0-12 %(w/v) | |
44063 | NaCl | positive | optimum | 0 %(w/v) | non-halophilic |
observation
- @ref: 44063
- observation: production of ansamitocin P-3
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
44063 | 17151 | xylitol | - | carbon source |
44063 | 17754 | glycerol | - | carbon source |
44063 | 17992 | sucrose | - | carbon source |
44063 | casein | - | hydrolysis | |
44063 | 28017 | starch | - | hydrolysis |
44063 | 53425 | tween 60 | - | hydrolysis |
44063 | 17196 | L-asparagine | - | nitrogen source |
44063 | 29991 | L-aspartate | - | nitrogen source |
44063 | 17561 | L-cysteine | - | nitrogen source |
44063 | 15971 | L-histidine | - | nitrogen source |
44063 | 16828 | L-tryptophan | - | nitrogen source |
44063 | 17895 | L-tyrosine | - | nitrogen source |
44063 | 17632 | nitrate | - | reduction |
44063 | 17108 | D-arabinose | + | carbon source |
44063 | 15824 | D-fructose | + | carbon source |
44063 | 12936 | D-galactose | + | carbon source |
44063 | 17634 | D-glucose | + | carbon source |
44063 | 16024 | D-mannose | + | carbon source |
44063 | 16988 | D-ribose | + | carbon source |
44063 | 17924 | D-sorbitol | + | carbon source |
44063 | 65327 | D-xylose | + | carbon source |
44063 | 62345 | L-rhamnose | + | carbon source |
44063 | 17266 | L-sorbose | + | carbon source |
44063 | 17716 | lactose | + | carbon source |
44063 | 17306 | maltose | + | carbon source |
44063 | 17268 | myo-inositol | + | carbon source |
44063 | 17272 | propionate | + | carbon source |
44063 | 16634 | raffinose | + | carbon source |
44063 | 53258 | sodium citrate | + | carbon source |
44063 | 27082 | trehalose | + | carbon source |
44063 | 62968 | cellulose | + | hydrolysis |
44063 | 53424 | tween 20 | + | hydrolysis |
44063 | 53423 | tween 40 | + | hydrolysis |
44063 | 53426 | tween 80 | + | hydrolysis |
44063 | 16449 | dl-alanine | + | nitrogen source |
44063 | 16467 | L-arginine | + | nitrogen source |
44063 | 29985 | L-glutamate | + | nitrogen source |
44063 | 17191 | L-isoleucine | + | nitrogen source |
44063 | 18019 | L-lysine | + | nitrogen source |
44063 | 16643 | L-methionine | + | nitrogen source |
44063 | 17295 | L-phenylalanine | + | nitrogen source |
44063 | 17115 | L-serine | + | nitrogen source |
44063 | 16857 | L-threonine | + | nitrogen source |
44063 | 16414 | L-valine | + | nitrogen source |
metabolite production
- @ref: 44063
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: no
fatty acid profile
fatty acids
@ref fatty acid percentage 44063 10-Methyl C17:0 1.97 44063 10-Methyl C18:0-TBSA 6.53 44063 C15:0 anteiso 1.81 44063 C17:0 anteiso 25.66 44063 C19:0 anteiso 0.65 44063 C14:0 0.17 44063 C16:0 2.39 44063 C16:1ω7c / C16:1ω6c 1.44 44063 C17:0 0.31 44063 C17:0 2OH 0.38 44063 C17:1ω8c 2.14 44063 C18:0 3.98 44063 C18:1ω7c / C18:1ω6c 0.15 44063 C18:1ω9c 20.13 44063 C14:0 iso 0.4 44063 C15:0 iso 1.03 44063 C16:0 iso 20.66 44063 C17:0 iso 6.68 44063 iso-C17:1-I / anteiso-C17:1-B 0.31 44063 iso-C17:1ω9c / 10-methyl-C16:0 0.52 44063 C18:0 iso 1.68 44063 C19:0 iso 0.61 44063 unkoωn 18.846/C19:1ω6c or C19:1ω 0.41 - type of FA analysis: whole cell analysis
- incubation medium: TSB
- incubation temperature: 30
- incubation time: 4
- incubation pH: 9
- library/peak naming table: TSBA6
- system: MIS MIDI
- method/protocol: Sasser, 1990
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|
24132 | desert soil | Xinjiang Province, Karamay | China | CHN | Asia | ||||
44063 | saline-alkali soil sample | Karamay, Xinjiang Province | China | CHN | Asia | ISP2 | adjusted to pH 10 with NaOH | 28 days | 30 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Desert |
#Condition | #Alkaline | |
#Condition | #Saline | |
#Environmental | #Terrestrial | #Soil |
Safety information
risk assessment
- @ref: 24132
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24132
- description: Nocardiopsis ansamitocini strain EGI 80425 16S ribosomal RNA gene, partial sequence
- accession: KR605109
- length: 1524
- database: ena
- NCBI tax ID: 1670832
GC content
- @ref: 24132
- GC-content: 70.2
- method: high performance liquid chromatography (HPLC)
External links
@ref: 24132
culture collection no.: DSM 103990, CGMCC 9969, KCTC 39605, EGI 80425
straininfo link
- @ref: 90689
- straininfo: 406421
literature
- topic: Phylogeny
- Pubmed-ID: 26486850
- title: Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis.
- authors: Zhang YG, Liu Q, Wang HF, Park DJ, Guo JW, Kim CJ, Zhang YM, Li WJ
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.000703
- year: 2015
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Maytansine/*analogs & derivatives/biosynthesis, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
24132 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103990 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103990) | |||
44063 | Yong-Guang Zhang, Qing Liu, Hong-Fei Wang, Dong-Jin Park, Jian-Wei Guo, Chang-Jin Kim, Yuan-Ming Zhang and Wen-Jun Li | Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis | 10.1099/ijsem.0.000703 | IJSEM 66: 230-235 2016 | 26486850 | |
90689 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID406421.1 |