Name: Leucobacter musarum CBX 152
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132272

Type strain:

Species: Leucobacter musarum

Strain Designation: CBX 152, Verde2, CBX152

Culture col. no.: DSM 27160, CIP 110721, JCM 31937

Strain history: CIP <- 2014, L. Clark, Oxford Univ., Oxford, UK: strain CBX152

NCBI tax ID(s): 1930747 (species)

SI-ID 403730

DSM 27160, CIP 110721

Section

Leucobacter musarum CBX 152 is a mesophilic, Gram-positive animal pathogen that was isolated from diseased nematode strain of genus Caenorhabditis.

Gram-positive
animal pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Leucobacter*
	Species	**Leucobacter musarum**
	Full Scientific Name (LPSN)	Leucobacter musarum Clark and Hodgkin 2015

Information on morphological and physiological properties Morphology

[Ref.: #121203]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #121203]	Cell shape	rod-shaped

[Ref.: #121203]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24061]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #24061]

Culture medium growth

[Ref.: #24061]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24061]

Culture medium composition

expand / minimize

[Ref.: #121203]

Culture medium

CIP Medium 72

[Ref.: #121203]

Culture medium growth

[Ref.: #121203]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #24061]			growth	28 °C
[Ref.: #67770]			growth	28 °C

[Ref.: #24061]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

[Ref.: #67770]

Observation

quinones: MK-10, MK-11

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24061]	Sample type/isolated from	diseased nematode strain of genus Caenorhabditis
[Ref.: #24061]	Host species	Caenorhabditis
[Ref.: #24061]	Geographic location (country and/or sea, region)	Santa Antao, Pico da Cruz (17° 6' 0'' N 25° 01' 45'' E)
[Ref.: #24061]	Country	Cape Verde
[Ref.: #24061]	Country ISO 3 Code	CPV
[Ref.: #24061]	Continent	Africa
[Ref.: #24061]	Geographic location	17.1000°/-25.0292°

[Ref.: #67770]	Sample type/isolated from	Nematodes from Santa Antao
[Ref.: #67770]	Country	Cape Verde
[Ref.: #67770]	Country ISO 3 Code	CPV
[Ref.: #67770]	Continent	Africa

[Ref.: #121203]	Sample type/isolated from	Environment, Soil
[Ref.: #121203]	Country	Cape Verde
[Ref.: #121203]	Country ISO 3 Code	CPV
[Ref.: #121203]	Continent	Africa
[Ref.: #121203]	Isolation date	2009
* marker position based on {}

Isolation sources categories

#Host

#Invertebrates (Other)

#Nematoda

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24061]	Pathogenicity (animal)	yes
[Ref.: #24061]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121203]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24061]	Leucobacter musarum subsp. musarum strain CBX152 16S ribosomal RNA gene, partial sequence	KF906845	1159	ENA	1461261 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leucobacter musarum subsp. musarum CBX152	GCA_001273845	contig	GenBank	1461261 tax ID
[Ref.: #66792]	Leucobacter sp. CBX152	1461261.3	wgs	PATRIC	1461261 tax ID	*

[Ref.: #24061]

GC-content

66.77 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Leucobacter sp. CBX152 PATRIC: 1461261.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leucobacter sp. CBX152 PATRIC: 1461261.3
[Ref.: #69480]	motile	no	89.176	no
[Ref.: #69480]	gram-positive	yes	91.853	no
[Ref.: #69480]	anaerobic	no	99.104	no
[Ref.: #69480]	halophile	no	74.98	no
[Ref.: #69480]	spore-forming	no	93.839	no
[Ref.: #69480]	thermophile	no	99.82	no
[Ref.: #69480]	glucose-util	yes	78.733	no
[Ref.: #69480]	aerobic	yes	94.372	no
[Ref.: #69480]	flagellated	no	95.956	no
[Ref.: #69480]	glucose-ferment	no	88.067	no

Availability in culture collections External links

[Ref.: #24061]

Culture collection no.

DSM 27160, CIP 110721, JCM 31937

[Ref.: #90619]

SI-ID 403730

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leucobacter musarum subsp. musarum sp. nov., subsp. nov., Leucobacter musarum subsp. japonicus subsp. nov., and Leucobacter celer subsp. astrifaciens subsp. nov., three nematopathogenic bacteria isolated from Caenorhabditis, with an emended description of Leucobacter celer.	Clark LC, Hodgkin J	Int J Syst Evol Microbiol	10.1099/ijsem.0.000523	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24061

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27160

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90619

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403730.1 )

#121203 Collection of Institut Pasteur ; Curators of the CIP; CIP 110721
* These data were automatically processed and therefore are not curated

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Leucobacter sp. CBX152

Leucobacter sp. CBX152

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