Frischella perrara (DSM 104328, ATCC BAA-2450, NCIMB 14821)

Name: Frischella perrara (DSM 104328, ATCC BAA-2450, NCIMB 14821)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132158

Type strain

Strain Designation PEB0191

Culture col. no. DSM 104328 ATCC BAA-2450 NCIMB 14821 PEB0191

NCBI tax ID(s) 1267021

Special Collections DSMZ Literature strains

History <- NCIMB <- P. Engel, Department of Ecology and Evolutionary Biology, Yale University, New Haven, USA; PEB0191

Links

Frischella perrara PEB0191 is an anaerobe, Gram-negative, rod-shaped bacterium that was isolated from homogenized gut of two worker bees .

Gram-negative rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 400937

LMG 32661,ATCC BAA-2450,NCIMB 14821,DSM 104328

@ref 20215

Last LPSN update 2026-02-09 11:31:38

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Orbales

Family Orbaceae

Genus Frischella

Species Frischella perrara

Full scientific name Frischella perrara Engel et al. 2013

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31004	negative	2 µm	0.5 µm	rod-shaped
125439	negative				98.4
125438	negative				96.979

Pigmentation

31004

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
23947	BHI MEDIUM (DSMZ Medium 215)	Medium recipe at MediaDive	Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
23947	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
23947	COLUMBIA BLOOD AGAR (DSMZ Medium 429)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base

Culture temp

@ref	Growth	Type	Temperature (°C)
23947	positive	growth	37
31004	positive	growth	24-42
31004	positive	optimum	37

Culture pH

@ref	Ability	Type	PH
31004	positive	optimum	7
31004	positive	growth	6.50-8.00

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31004	anaerobe
125439	obligate aerobe	96.7

Spore formation

@ref	Spore formation	Confidence
125439		95.3

Observation

31004

Observationaggregates in clumps

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31004	28757 ChEBI	fructose	+	carbon source
31004	17234 ChEBI	glucose	+	carbon source
31004	37684 ChEBI	mannose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
31004	catalase	+	1.11.1.6

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	L-lactaldehyde degradation	100	3 of 3
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	chorismate metabolism	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	propanol degradation	85.71	6 of 7
66794	heme metabolism	85.71	12 of 14
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	NAD metabolism	83.33	15 of 18
66794	peptidoglycan biosynthesis	80	12 of 15
66794	threonine metabolism	80	8 of 10
66794	glutathione metabolism	78.57	11 of 14
66794	phenylalanine metabolism	76.92	10 of 13
66794	vitamin B1 metabolism	76.92	10 of 13
66794	alanine metabolism	75.86	22 of 29
66794	CMP-KDO biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	butanoate fermentation	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	proline metabolism	72.73	8 of 11
66794	pentose phosphate pathway	72.73	8 of 11
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	photosynthesis	71.43	10 of 14
66794	cyanate degradation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	flavin biosynthesis	66.67	10 of 15
66794	acetoin degradation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	purine metabolism	64.89	61 of 94
66794	glycolysis	64.71	11 of 17
66794	glutamate and glutamine metabolism	64.29	18 of 28
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	methionine metabolism	61.54	16 of 26
66794	leucine metabolism	61.54	8 of 13
66794	glycogen metabolism	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	d-mannose degradation	55.56	5 of 9
66794	pyrimidine metabolism	55.56	25 of 45
66794	non-pathway related	55.26	21 of 38
66794	histidine metabolism	51.72	15 of 29
66794	oxidative phosphorylation	51.65	47 of 91
66794	lipid metabolism	51.61	16 of 31
66794	ethanol fermentation	50	1 of 2
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	suberin monomers biosynthesis	50	1 of 2
66794	gluconeogenesis	50	4 of 8
66794	toluene degradation	50	2 of 4
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	cysteine metabolism	50	9 of 18
66794	arginine metabolism	50	12 of 24
66794	ketogluconate metabolism	50	4 of 8
66794	quinate degradation	50	1 of 2
66794	lysine metabolism	47.62	20 of 42
66794	tryptophan metabolism	47.37	18 of 38
66794	urea cycle	46.15	6 of 13
66794	citric acid cycle	42.86	6 of 14
66794	tyrosine metabolism	42.86	6 of 14
66794	glycine metabolism	40	4 of 10
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	degradation of sugar alcohols	37.5	6 of 16
66794	metabolism of disaccharids	36.36	4 of 11
66794	vitamin B6 metabolism	36.36	4 of 11
66794	IAA biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	enterobactin biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	sphingosine metabolism	33.33	2 of 6
66794	nitrate assimilation	33.33	3 of 9
66794	degradation of pentoses	32.14	9 of 28
66794	ascorbate metabolism	31.82	7 of 22
66794	sulfate reduction	30.77	4 of 13
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	lactate fermentation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	cyclohexanol degradation	25	1 of 4
66794	alginate biosynthesis	25	1 of 4
66794	phenol degradation	25	5 of 20
66794	glycogen biosynthesis	25	1 of 4
66794	allantoin degradation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
23947	homogenized gut of two worker bees (Apis mellifera)	Apis mellifera	lab-raised hives at New Haven, USA	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence JX878306 (>99% sequence identity) for Frischella perrara subclade from Microbeatlas

Aquatic Samples	759
Soil Samples	194
Animal Samples	4746
Plant Samples	120
Total Samples	5819

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
23947	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM80727v1 assembly for Frischella perrara PEB0191	complete	GCA_000807275	1267021.3	2630968947	1267021	98.76
66792	Frischella perrara PEB0191	complete			2515154034	1267021	93.03
66792	ASM318204v1 assembly for Frischella perrara DSM 104328	scaffold	GCA_003182045	1267021.8	2756170266	1267021	64.17

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
23947	Frischella perrara strain PEB0191 16S ribosomal RNA gene, partial sequence	JX878306	1534		1267021

GC content

31004

GC-content (mol%)33.9

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Frischella perrara PEB0191
PATRIC: 1267021.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.70	no
125439	gram_stain	BacteriaNetⓘ	negative	98.40	no
125439	motility	BacteriaNetⓘ	no	63.60	no
125439	spore_formation	BacteriaNetⓘ	no	95.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Frischella perrara PEB0191
NCBI: GCA_000807275.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.98	yes
125438	anaerobic	anaerobicⓘ	no	52.45	yes
125438	aerobic	aerobicⓘ	no	83.32	no
125438	spore-forming	spore-formingⓘ	no	89.24	no
125438	thermophilic	thermophileⓘ	no	97.63	yes
125438	flagellated	motile2+ⓘ	no	67.99	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	The Gut Microbiota Can Provide Viral Tolerance in the Honey Bee.	Dosch C, Manigk A, Streicher T, Tehel A, Paxton RJ, Tragust S.	Microorganisms	10.3390/microorganisms9040871	2021
Phylogeny	Gut symbionts from distinct hosts exhibit genotoxic activity via divergent colibactin biosynthesis pathways.	Engel P, Vizcaino MI, Crawford JM	Appl Environ Microbiol	10.1128/AEM.03283-14	2015
Phylogeny	Frischella japonica sp. nov., an anaerobic member of the Orbales in the Gammaproteobacteria, isolated from the gut of the eastern honey bee, Apis cerana japonica Fabricius.	Wolter LA, Suenami S, Miyazaki R	Int J Syst Evol Microbiol	10.1099/ijsem.0.004712	2021
Phylogeny	Frischella perrara gen. nov., sp. nov., a gammaproteobacterium isolated from the gut of the honeybee, Apis mellifera.	Engel P, Kwong WK, Moran NA	Int J Syst Evol Microbiol	10.1099/ijs.0.049569-0	2013

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23947	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104328
#27334	IJSEM 3646 2013 ( DOI 10.1099/ijs.0.049569-0 , PubMed 23606484 )
#31004	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27334
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Frischella perrara (DSM 104328, ATCC BAA-2450, NCIMB 14821)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Enzymes

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000807275

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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