Name: Frischella perrara PEB0191
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132158

Type strain:

Species: Frischella perrara

Strain Designation: PEB0191

Culture col. no.: DSM 104328, ATCC BAA-2450, NCIMB 14821

Strain history: <- NCIMB <- P. Engel, Department of Ecology and Evolutionary Biology, Yale University, New Haven, USA; PEB0191

NCBI tax ID(s): 1267021 (species)

SI-ID 400937

DSM 104328

Special Collections

Section

Frischella perrara PEB0191 is an anaerobe, Gram-negative, rod-shaped bacterium that was isolated from homogenized gut of two worker bees .

Gram-negative
rod-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Orbales*
	Family	*Orbaceae*
	Genus	*Frischella*
	Species	**Frischella perrara**
	Full Scientific Name (LPSN)	Frischella perrara Engel et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #31004]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %96.979

[Ref.: #31004]	Cell length	2 µm

[Ref.: #31004]	Cell width	0.5 µm

[Ref.: #31004]	Cell shape	rod-shaped

[Ref.: #31004]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23947]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #23947]

Culture medium growth

[Ref.: #23947]

Culture medium link

Medium recipe at MediaDive

[Ref.: #23947]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #23947]	growth	35 °C
[Ref.: #31004]	growth	24-42 °C
[Ref.: #31004]	optimum	37 °C

	pH	Kind of pH		pH
[Ref.: #31004]			optimum	7
[Ref.: #31004]			growth	6.50-8.00

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31004]

Oxygen tolerance

anaerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

[Ref.: #31004]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31004]	fructose ChEBI	+	carbon source
[Ref.: #31004]	glucose ChEBI	+	carbon source
[Ref.: #31004]	mannose ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #31004]		catalase	+	1.11.1.6

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	tetrahydrofolate metabolism	92.86	13 of 14
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	heme metabolism	85.71	12 of 14
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	glutathione metabolism	78.57	11 of 14
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	alanine metabolism	75.86	22 of 29
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	proline metabolism	72.73	8 of 11
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	photosynthesis	71.43	10 of 14
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	flavin biosynthesis	66.67	10 of 15
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	purine metabolism	64.89	61 of 94
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	C4 and CAM-carbon fixation	62.5	5 of 8
[Ref.: #66794]	methionine metabolism	61.54	16 of 26
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	isoprenoid biosynthesis	57.69	15 of 26
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	pyrimidine metabolism	55.56	25 of 45
[Ref.: #66794]	non-pathway related	55.26	21 of 38
[Ref.: #66794]	histidine metabolism	51.72	15 of 29
[Ref.: #66794]	oxidative phosphorylation	51.65	47 of 91
[Ref.: #66794]	lipid metabolism	51.61	16 of 31
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	gluconeogenesis	50	4 of 8
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	lysine metabolism	47.62	20 of 42
[Ref.: #66794]	tryptophan metabolism	47.37	18 of 38
[Ref.: #66794]	urea cycle	46.15	6 of 13
[Ref.: #66794]	citric acid cycle	42.86	6 of 14
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	ethylmalonyl-CoA pathway	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	degradation of hexoses	38.89	7 of 18
[Ref.: #66794]	degradation of sugar alcohols	37.5	6 of 16
[Ref.: #66794]	metabolism of disaccharids	36.36	4 of 11
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	degradation of pentoses	32.14	9 of 28
[Ref.: #66794]	ascorbate metabolism	31.82	7 of 22
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	3-phenylpropionate degradation	26.67	4 of 15
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	alginate biosynthesis	25	1 of 4
[Ref.: #66794]	phenol degradation	25	5 of 20
[Ref.: #66794]	glycogen biosynthesis	25	1 of 4
[Ref.: #66794]	allantoin degradation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23947]	Sample type/isolated from	homogenized gut of two worker bees (Apis mellifera)
[Ref.: #23947]	Host species	Apis mellifera
[Ref.: #23947]	Geographic location (country and/or sea, region)	lab-raised hives at New Haven, USA
[Ref.: #23947]	Country	USA
[Ref.: #23947]	Country ISO 3 Code	USA
[Ref.: #23947]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX878306 (>99% sequence identity) for Frischella perrara subclade from Microbeatlas

Aquatic Samples	759
Soil Samples	194
Animal Samples	4746
Plant Samples	120
Total Samples	5819

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #23947]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23947]	Frischella perrara strain PEB0191 16S ribosomal RNA gene, partial sequence	JX878306	1534	GenBank ENA	1267021 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Frischella perrara PEB0191	GCA_000807275	complete	GenBank ENA	1267021 tax ID	*
[Ref.: #66792]	Frischella perrara DSM 104328	GCA_003182045	scaffold	GenBank ENA	1267021 tax ID	*
[Ref.: #66792]	Frischella perrara PEB0191	1267021.3	complete	PATRIC	1267021 tax ID	*
[Ref.: #66792]	Frischella perrara strain DSM 104328	1267021.8	wgs	PATRIC	1267021 tax ID	*
[Ref.: #66792]	Frischella perrara PEB0191	2515154034	draft	JGI IMG/M	1267021 tax ID	*
[Ref.: #66792]	Frischella perrara DSM 104328	2756170266	draft	JGI IMG/M	1267021 tax ID	*

[Ref.: #31004]

GC-content

33.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Frischella perrara PEB0191 NCBI: GCA_000807275.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Frischella perrara PEB0191 NCBI: GCA_000807275.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	96.979	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	52.449	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	83.32	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	89.239	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.627	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	67.988	no

Availability in culture collections External links

[Ref.: #23947]

Culture collection no.

DSM 104328, ATCC BAA-2450, NCIMB 14821

[Ref.: #90514]

SI-ID 400937

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Frischella japonica sp. nov., an anaerobic member of the Orbales in the Gammaproteobacteria, isolated from the gut of the eastern honey bee, Apis cerana japonica Fabricius.	Wolter LA, Suenami S, Miyazaki R	Int J Syst Evol Microbiol	10.1099/ijsem.0.004712	2021	*
Phylogeny	Gut symbionts from distinct hosts exhibit genotoxic activity via divergent colibactin biosynthesis pathways.	Engel P, Vizcaino MI, Crawford JM	Appl Environ Microbiol	10.1128/AEM.03283-14	2015	*
Phylogeny	Frischella perrara gen. nov., sp. nov., a gammaproteobacterium isolated from the gut of the honeybee, Apis mellifera.	Engel P, Kwong WK, Moran NA	Int J Syst Evol Microbiol	10.1099/ijs.0.049569-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23947

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104328

#31004

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27334 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90514

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400937.1 )

#27334

IJSEM 3646 2013 ( DOI 10.1099/ijs.0.049569-0 , PubMed 23606484 )

* These data were automatically processed and therefore are not curated

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