Strain identifier
BacDive ID: 132158
Type strain: 
Species: Frischella perrara
Strain Designation: PEB0191
Strain history: <- NCIMB <- P. Engel, Department of Ecology and Evolutionary Biology, Yale University, New Haven, USA; PEB0191
NCBI tax ID(s): 1267021 (species)
version 8.1 (current version)
General
@ref: 23947
BacDive-ID: 132158
DSM-Number: 104328
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped
description: Frischella perrara PEB0191 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from homogenized gut of two worker bees .
NCBI tax id
- NCBI tax id: 1267021
- Matching level: species
strain history
- @ref: 23947
- history: <- NCIMB <- P. Engel, Department of Ecology and Evolutionary Biology, Yale University, New Haven, USA; PEB0191
doi: 10.13145/bacdive132158.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Orbales
- family: Orbaceae
- genus: Frischella
- species: Frischella perrara
- full scientific name: Frischella perrara Engel et al. 2013
@ref: 23947
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Orbales
family: Orbaceae
genus: Frischella
species: Frischella perrara
full scientific name: Frischella perrara Engel et al. 2013
strain designation: PEB0191
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 31004 | negative | 2 µm | 0.5 µm | rod-shaped | ||
| 69480 | yes | 93.13 | ||||
| 69480 | negative | 99.806 |
pigmentation
- @ref: 31004
- production: yes
Culture and growth conditions
culture medium
- @ref: 23947
- name: BHI MEDIUM (DSMZ Medium 215)
- growth: yes
- link: https://mediadive.dsmz.de/medium/215
- composition: Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 23947 | positive | growth | 35 | mesophilic |
| 31004 | positive | growth | 24-42 | |
| 31004 | positive | optimum | 37 | mesophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 31004 | positive | growth | 6.50-8.00 |
| 31004 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 31004
- oxygen tolerance: anaerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.997 |
observation
- @ref: 31004
- observation: aggregates in clumps
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31004 | 28757 | fructose | + | carbon source |
| 31004 | 17234 | glucose | + | carbon source |
| 31004 | 37684 | mannose | + | carbon source |
enzymes
- @ref: 31004
- value: catalase
- activity: +
- ec: 1.11.1.6
Isolation, sampling and environmental information
isolation
- @ref: 23947
- sample type: homogenized gut of two worker bees (Apis mellifera)
- host species: Apis mellifera
- geographic location: lab-raised hives at New Haven, USA
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Arthropoda | #Insecta |
| #Host Body-Site | #Gastrointestinal tract |
taxonmaps
- @ref: 69479
- File name: preview.99_5119.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_84;96_2490;97_3041;98_3819;99_5119&stattab=map
- Last taxonomy: Frischella perrara subclade
- 16S sequence: JX878306
- Sequence Identity:
- Total samples: 5819
- soil counts: 194
- aquatic counts: 759
- animal counts: 4746
- plant counts: 120
Safety information
risk assessment
- @ref: 23947
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 23947
- description: Frischella perrara strain PEB0191 16S ribosomal RNA gene, partial sequence
- accession: JX878306
- length: 1534
- database: ena
- NCBI tax ID: 1267021
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Frischella perrara PEB0191 | GCA_000807275 | complete | ncbi | 1267021 |
| 66792 | Frischella perrara DSM 104328 | GCA_003182045 | scaffold | ncbi | 1267021 |
| 66792 | Frischella perrara PEB0191 | 1267021.3 | complete | patric | 1267021 |
| 66792 | Frischella perrara strain DSM 104328 | 1267021.8 | wgs | patric | 1267021 |
| 66792 | Frischella perrara PEB0191 | 2515154034 | draft | img | 1267021 |
| 66792 | Frischella perrara DSM 104328 | 2756170266 | draft | img | 1267021 |
| 66792 | Frischella perrara PEB0191 | 2630968947 | complete | img | 1267021 |
GC content
- @ref: 31004
- GC-content: 33.9
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | yes | 73.709 | no |
| flagellated | no | 66.79 | no |
| gram-positive | no | 97.718 | yes |
| anaerobic | no | 78.601 | yes |
| aerobic | no | 92.204 | yes |
| halophile | no | 57.688 | no |
| spore-forming | no | 97.868 | no |
| thermophile | no | 99.582 | yes |
| glucose-util | yes | 69.187 | yes |
| glucose-ferment | yes | 64.704 | no |
External links
@ref: 23947
culture collection no.: DSM 104328, ATCC BAA 2450, NCIMB 14821
straininfo link
- @ref: 90514
- straininfo: 400937
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 23606484 | Frischella perrara gen. nov., sp. nov., a gammaproteobacterium isolated from the gut of the honeybee, Apis mellifera. | Engel P, Kwong WK, Moran NA | Int J Syst Evol Microbiol | 10.1099/ijs.0.049569-0 | 2013 | Animals, Bacterial Typing Techniques, Base Composition, Bees/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Gammaproteobacteria/*classification/genetics/isolation & purification, Gastrointestinal Tract/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis | Genetics |
| Phylogeny | 25527542 | Gut symbionts from distinct hosts exhibit genotoxic activity via divergent colibactin biosynthesis pathways. | Engel P, Vizcaino MI, Crawford JM | Appl Environ Microbiol | 10.1128/AEM.03283-14 | 2015 | Animals, Bees, Biosynthetic Pathways, DNA Damage/drug effects, Enterobacteriaceae/genetics/metabolism, Escherichia coli/genetics/metabolism, Gammaproteobacteria/classification/genetics/isolation & purification/*metabolism, Gastrointestinal Tract/*microbiology, Genomic Islands, Humans, Molecular Sequence Data, Peptides/*metabolism/toxicity, Phylogeny, Polyketides/*metabolism/toxicity, Rhodobacteraceae/classification/genetics/isolation & purification/*metabolism, Species Specificity, *Symbiosis | Metabolism |
| Phylogeny | 33616516 | Frischella japonica sp. nov., an anaerobic member of the Orbales in the Gammaproteobacteria, isolated from the gut of the eastern honey bee, Apis cerana japonica Fabricius. | Wolter LA, Suenami S, Miyazaki R | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004712 | 2021 |
Reference
| @id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||||
| 23947 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-104328 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104328) | ||||
| 31004 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27334 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | ||||
| 90514 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID400937.1 |