Name: Actinokineospora cibodasensis ID 03-0784
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13208

Type strain:

Species: Actinokineospora cibodasensis

Strain Designation: ID 03-0784

Culture col. no.: DSM 45658, BTCC B555, NBRC 104212

Strain history: <- NBRC; NBRC 104212 <- P. Lisdiyanti (LIPI)and K. Ando(NITE); ID 03-0784

NCBI tax ID(s): 547057 (species)

SI-ID 400394

DSM 45658

Section

Actinokineospora cibodasensis ID 03-0784 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from litter.

Gram-positive
motile
rod-shaped
spore-forming
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Actinokineospora*
	Species	**Actinokineospora cibodasensis**
	Full Scientific Name (LPSN)	Actinokineospora cibodasensis Lisdiyanti et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29447]	Gram stain	positive

[Ref.: #29447]	Cell width	1.5-1.8 µm

[Ref.: #29447]	Cell shape	rod-shaped

[Ref.: #29447]	Motility	yes

[Ref.: #29447]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18015]

Culture medium

BENNETT'S AGAR (DSMZ Medium 548)

[Ref.: #18015]

Culture medium growth

[Ref.: #18015]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18015]

Culture medium composition

expand / minimize

[Ref.: #18015]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #18015]

Culture medium growth

[Ref.: #18015]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18015]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #18015]	growth	28 °C
[Ref.: #29447]	growth	25-28 °C
[Ref.: #29447]	optimum	25-28 °C

[Ref.: #18015]	Temperature range	mesophilic
[Ref.: #29447]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29447]			optimum	7
[Ref.: #29447]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29447]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29447]		NaCl		growth	1	%
[Ref.: #29447]		NaCl		optimum	1	%

[Ref.: #29447]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #29447]	esculin ChEBI	+	hydrolysis
[Ref.: #29447]	fructose ChEBI	+	carbon source
[Ref.: #29447]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #29447]	glucose ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #29447]	maltose ChEBI	+	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #29447]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #29447]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #29447]	sucrose ChEBI	+	carbon source
[Ref.: #29447]	trehalose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #18015]	25157	+	-	-	-	-	+/-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18015]	14280	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18015]	Sample type/isolated from	litter
[Ref.: #18015]	Geographic location (country and/or sea, region)	West Java, Cibodas Botanical Garden
[Ref.: #18015]	Country	Indonesia
[Ref.: #18015]	Country ISO 3 Code	IDN
[Ref.: #18015]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	-
#Host Body Product	#Plant	#Plant litter (Forest)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18015]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18015]	Actinokineospora cibodasensis gene for 16S rRNA, partial sequence, strain: NBRC 104212	AB447489	1472	ENA	547057 tax ID

[Ref.: #18015]

GC-content

71.3 mol%

Availability in culture collections External links

[Ref.: #18015]

Culture collection no.

DSM 45658, BTCC B555, NBRC 104212

[Ref.: #82416]

SI-ID 400394

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alloactinosynnema iranicum sp. nov., a rare actinomycete isolated from a hypersaline wetland, and emended description of the genus Alloactinosynnema.	Nikou MM, Ramezani M, Amoozegar MA, Fazeli SAS, Schumann P, Sproer C, Sanchez-Porro C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.049189-0	2014	*
Phylogeny	Actinokineospora baliensis sp. nov., Actinokineospora cibodasensis sp. nov. and Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter.	Lisdiyanti P, Otoguro M, Ratnakomala S, Lestari Y, Hastuti RD, Triana E, Katsuhiko A, Widyastuti Y	Int J Syst Evol Microbiol	10.1099/ijs.0.013276-0	2009	*

References

#18015

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45658

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29447

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25853 (see below)

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#82416

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400394.1 )

#25853

IJSEM 2331 2010 ( DOI 10.1099/ijs.0.013276-0 , PubMed 19933586 )

* These data were automatically processed and therefore are not curated

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