Actinokineospora riparia (DSM 44259, ATCC 49499, CCRC 13450)

Name: Actinokineospora riparia (DSM 44259, ATCC 49499, CCRC 13450)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13203

Type strain

Culture col. no. DSM 44259 ATCC 49499 CCRC 13450 IFO 14541 JCM 7471 12 more

NCBI tax ID(s) 39846

Special Collections DSMZ JCM KCTC Wink compendium JMRC

History DSMZ <- IFO <- T. Hasegawa, C-39162 JCM <-- IFO 14541 <-- T. Hasegawa C-39162.

Links

Actinokineospora riparia DSM 44259 is a spore-forming bacterium that was isolated from soil.

spore-forming 16S sequence Bacteria

Name and taxonomic classification

SI-ID 125804

MTCC 2712,ATCC 49499,IFO 14541,JCM 7471,DSM 44259,CCRC 13450,NCIMB 13255,NRRL B-16432,VKM Ac-1980,KCTC 9291,NBRC 14541,BCRC 13450,KACC 20059,DSM 33452

@ref 20215

Last LPSN update 2026-05-29 08:27:59

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Actinokineospora

Species Actinokineospora riparia

Full scientific name Actinokineospora riparia Hasegawa 1988

Morphology

Colony morphology

@ref	Colony color	Incubation period	Medium used
20033	Yellow orange (2000)	10-14 days	ISP 2

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20033		ISP 2
20033		ISP 3
20033		ISP 4
20033		ISP 5
20033		ISP 6
20033		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
11631	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20033	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
11631	positive	growth	28
20033	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Spore formation

@ref	Spore description	Spore formation
20033	Formation of spore chains, spore surface smooth

Halophily

@ref	Salt	Growth	Tested relation	Concentration
20033	NaCl	positive	growth	0 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68368	25094 ChEBI	lysine	+	degradation	from API 20E
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
20033	-	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11631	soil	Shiga Prefecture, Ado River	Japan	JPN	Asia
67770	Soil, the Ado River	Shiga Pref.	Japan	JPN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11631	1	Risk group (German classification) According to TRBA 466
20033	1	German classification According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	A.riparia (IFO 14541) 16S ribosomal RNA (partial)	X76953	1426		39846
20218	Actinokineospora riparia strain NRRL B-16432T 16S ribosomal RNA gene, partial sequence	AF114802	1502		39846

GC content

@ref	GC-content (mol%)	Method
11631	68.9	high performance liquid chromatography (HPLC)
11631	72.0	thermal denaturation, midpoint method (Tm)
67770	72	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Distribution and phylogenetic analysis of family 19 chitinases in Actinobacteria.	Kawase T, Saito A, Sato T, Kanai R, Fujii T, Nikaidou N, Miyashita K, Watanabe T.	Appl Environ Microbiol	10.1128/aem.70.2.1135-1144.2004	2004
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018

References

#11631	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44259
#20033	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinokineospora riparia (DSM 44259, ATCC 49499, CCRC 13450)

Name and taxonomic classification

Morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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