Name: Thermobifida alba A-0077
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132007

Type strain:

Species: Thermobifida alba

Strain Designation: A-0077, 1900

Culture col. no.: DSM 43795, CECT 3324, CIP 105591, CUB 1002, CUB 432, DSM 43310, IFM 1271, IFO (now NBRC) 15853, IFO (now NBRC) 16095, IMET 9528, IMSNU 22187, JCM 3077, KCC A-0077, KCTC 9618, NBRC 15853, NBRC 16095, NCIB 10169, IPV 1900, IFO 15853, IFO 16095, NCIMB 10169

Strain history: CIP <- 1998, JCM <- KCC: strain A-0077 <- K. Locci, IPV: strain 1900

NCBI tax ID(s): 53522 (species)

SI-ID 50429

CIP 105591, DSM 43795, IFO 16095, JCM 3077, IMET 9528, NCIMB 10169, NCIB 10169, DSM 43310, IFO 15853, IFO10169, CECT 3324, KCTC 9618, NBRC 15853, NBRC 16095

Other strains from Thermobifida alba

T. alba T-3, DSM 43185, ATCC 27644, KCC ...

Section

Thermobifida alba A-0077 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from garden soil.

Gram-positive
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Nocardiopsidaceae*
	Genus	*Thermobifida*
	Species	**Thermobifida alba**
	Full Scientific Name (LPSN)	Thermobifida alba (Locci et al. 1967) Zhang et al. 1998

	Synonym	"Actinobifida* alba"*
	Synonym	Thermomonospora mesouviformis
	Synonym	Thermomonospora alba

Information on morphological and physiological properties Morphology

[Ref.: #116180]	Gram stain	positive

[Ref.: #116180]	Cell shape	rod-shaped

[Ref.: #116180]	Motility	no

Multimedia content

[Ref.: #11250]

Caption:

Medium 65 37°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #11250]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #11250]

Culture medium composition

expand / minimize

[Ref.: #39322]

Culture medium

MEDIUM 287 - for Thermobifida alba

[Ref.: #39322]

Culture medium growth

[Ref.: #39322]

Culture medium composition

expand / minimize

[Ref.: #11250]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11250]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

[Ref.: #116180]

Culture medium

CIP Medium 287

[Ref.: #116180]

Culture medium growth

[Ref.: #116180]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11250]		growth	37 °C
[Ref.: #39322]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #116180]		growth	22-41 °C
[Ref.: #116180]	no	growth	5 °C
[Ref.: #116180]	no	growth	15 °C

[Ref.: #11250]	Temperature range	mesophilic
[Ref.: #39322]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #116180]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116180]

Oxygen tolerance

obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #116180]	NaCl		growth	2	%
[Ref.: #116180]	NaCl	no	growth	0	%
[Ref.: #116180]	NaCl	no	growth	4	%
[Ref.: #116180]	NaCl	no	growth	6	%
[Ref.: #116180]	NaCl	no	growth	8	%
[Ref.: #116180]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-10(H₆), MK-10(H₈), MK-10(H₁₀), MK-11(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116180]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116180]	glucose ChEBI	-	degradation
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116180]	nitrate ChEBI	-	reduction
[Ref.: #116180]	nitrate ChEBI	-	respiration
[Ref.: #116180]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #116180]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #116180]		acetoin ChEBI	-
[Ref.: #116180]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #116180]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116180]	amylase	+
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116180]	caseinase	+	3.4.21.50
[Ref.: #116180]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #116180]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116180]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #116180]	gelatinase	+
[Ref.: #116180]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #116180]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116180]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116180]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116180]	oxidase	-
[Ref.: #116180]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116180]	tryptophan deaminase	-
[Ref.: #116180]	tween esterase	+
[Ref.: #116180]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #116180]	10134	not determinedn.d.	-	-	-	-	+/-	+/-	-	-	-	-	+/-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	+	-	+/-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+/-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116180]	8822	-	+	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11250]	Sample type/isolated from	garden soil
[Ref.: #11250]	Geographic location (country and/or sea, region)	Milano
[Ref.: #11250]	Country	Italy
[Ref.: #11250]	Country ISO 3 Code	ITA
[Ref.: #11250]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Garden soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Milan
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe

[Ref.: #116180]	Sample type/isolated from	Environment, Garden soil
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Garden

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF002260 (>99% sequence identity) for Thermobifida from Microbeatlas

Aquatic Samples	1
Soil Samples	29
Animal Samples	6
Plant Samples	3
Total Samples	39

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11250]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116180]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11250]	Thermomonospora alba 16S ribosomal RNA gene, partial sequence	AF002260	1468	ENA	53522 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermobifida alba DSM 43795	GCA_023208015	complete	GenBank	53522 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermobifida alba DSM 43795 NCBI: GCA_023208015.1
[Ref.: #69480]	flagellated	no	97.872	no
[Ref.: #69480]	gram-positive	yes	90.08	no
[Ref.: #69480]	anaerobic	no	98.628	no
[Ref.: #69480]	aerobic	yes	83.886	no
[Ref.: #69480]	halophile	no	63.276	no
[Ref.: #69480]	spore-forming	yes	89.767	no
[Ref.: #69480]	motile	no	93.631	no
[Ref.: #69480]	glucose-ferment	no	92.675	no
[Ref.: #69480]	thermophile	no	92.52	yes
[Ref.: #69480]	glucose-util	yes	84.326	no

Availability in culture collections External links

[Ref.: #11250]

Culture collection no.

DSM 43795, CECT 3324, CIP 105591, CUB 1002, CUB 432, DSM 43310, IFM 1271, IFO (now NBRC) 15853, IFO (now NBRC) 16095, IMET 9528, IMSNU 22187, JCM 3077, KCC A-0077, KCTC 9618, NBRC 15853, NBRC 16095, NCIB 10169, IPV 1900, IFO 15853, IFO 16095, NCIMB 10169

[Ref.: #90393]

SI-ID 50429

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Biotransformation of cladribine by a nanostabilized extremophilic biocatalyst.	Rivero CW, De Benedetti EC, Sambeth J, Trelles JA	J Biotechnol	10.1016/j.jbiotec.2020.08.012	2020	*
Phylogeny	Thermobifida cellulolytica sp. nov., a novel lignocellulose-decomposing actinomycete.	Kukolya J, Nagy I, Laday M, Toth E, Oravecz O, Marialigeti K, Hornok L	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1193	2002	*

References

#11250

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43795

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39322 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#90393

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID50429.1 )

#116180 Collection of Institut Pasteur ; Curators of the CIP; CIP 105591
* These data were automatically processed and therefore are not curated

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Thermobifida alba DSM 43795

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