Sulfurifustis variabilis (DSM 100313, NBRC 110942, skN76)

Name: Sulfurifustis variabilis (DSM 100313, NBRC 110942, skN76)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 131448

Type strain

Strain Designation skN76

Culture col. no. DSM 100313 NBRC 110942 skN76

NCBI tax ID(s) 1675686

Special Collections DSMZ

History <- T. Watanabe, Univ. Hokkaido, Japan; skN76 <- T. Watanabe; {2014}

Links

Sulfurifustis variabilis skN76 is a microaerophile bacterium that was isolated from sediment of a freshwater lake.

microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 398924

NBRC 110942,DSM 100313

@ref 20215

Last LPSN update 2026-02-09 11:31:28

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Acidiferrobacterales

Family Acidiferrobacteraceae

Genus Sulfurifustis

Species Sulfurifustis variabilis

Full scientific name Sulfurifustis variabilis Kojima et al. 2015

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	96.2
125438	negative	96.75

Colony morphology

22675

Incubation period>14 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
22675	SULFURIFUSTIS MEDIUM (DSMZ Medium 1277a)		Medium recipe at MediaDive	Name: SULFURIFUSTIS MEDIUM (DSMZ Medium 1277a) Composition: Na2S2O3 x 5 H2O 2.49252 g/l Na2CO3 0.997009 g/l MgCl2 x 6 H2O 0.199402 g/l CaCl2 x 2 H2O 0.0997009 g/l (NH4)2SO4 0.0997009 g/l KH2PO4 0.0997009 g/l KCl 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
22675	positive	growth	40

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
22675	microaerophile
125439	aerobe	93.8

Spore formation

@ref	Spore formation	Confidence
125439		94

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	ethanol fermentation	100	2 of 2
66794	aspartate and asparagine metabolism	100	9 of 9
66794	palmitate biosynthesis	100	22 of 22
66794	ppGpp biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	cyanate degradation	100	3 of 3
66794	lipoate biosynthesis	100	5 of 5
66794	biotin biosynthesis	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	ceramide biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	denitrification	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	glycine betaine biosynthesis	100	5 of 5
66794	hydrogen production	100	5 of 5
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	adipate degradation	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	ubiquinone biosynthesis	100	7 of 7
66794	photosynthesis	92.86	13 of 14
66794	valine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	heme metabolism	85.71	12 of 14
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	phenylalanine metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	pentose phosphate pathway	81.82	9 of 11
66794	starch degradation	80	8 of 10
66794	glycogen metabolism	80	4 of 5
66794	purine metabolism	79.79	75 of 94
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	lipid metabolism	77.42	24 of 31
66794	vitamin B1 metabolism	76.92	10 of 13
66794	leucine metabolism	76.92	10 of 13
66794	glycogen biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	ketogluconate metabolism	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	peptidoglycan biosynthesis	73.33	11 of 15
66794	methionine metabolism	73.08	19 of 26
66794	metabolism of disaccharids	72.73	8 of 11
66794	proline metabolism	72.73	8 of 11
66794	glycolysis	70.59	12 of 17
66794	propionate fermentation	70	7 of 10
66794	threonine metabolism	70	7 of 10
66794	alanine metabolism	68.97	20 of 29
66794	pyrimidine metabolism	68.89	31 of 45
66794	octane oxidation	66.67	2 of 3
66794	flavin biosynthesis	66.67	10 of 15
66794	acetoin degradation	66.67	2 of 3
66794	nitrate assimilation	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	IAA biosynthesis	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	non-pathway related	65.79	25 of 38
66794	citric acid cycle	64.29	9 of 14
66794	glutathione metabolism	64.29	9 of 14
66794	vitamin B6 metabolism	63.64	7 of 11
66794	oxidative phosphorylation	61.54	56 of 91
66794	sulfate reduction	61.54	8 of 13
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	propanol degradation	57.14	4 of 7
66794	degradation of sugar alcohols	56.25	9 of 16
66794	4-hydroxymandelate degradation	55.56	5 of 9
66794	histidine metabolism	55.17	16 of 29
66794	arginine metabolism	54.17	13 of 24
66794	tryptophan metabolism	52.63	20 of 38
66794	lysine metabolism	52.38	22 of 42
66794	androgen and estrogen metabolism	50	8 of 16
66794	Entner Doudoroff pathway	50	5 of 10
66794	degradation of hexoses	50	9 of 18
66794	toluene degradation	50	2 of 4
66794	resorcinol degradation	50	1 of 2
66794	cysteine metabolism	50	9 of 18
66794	butanoate fermentation	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	phenylmercury acetate degradation	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	tyrosine metabolism	50	7 of 14
66794	phenol degradation	50	10 of 20
66794	glycine metabolism	50	5 of 10
66794	coenzyme M biosynthesis	50	5 of 10
66794	vitamin B12 metabolism	47.06	16 of 34
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	degradation of sugar acids	44	11 of 25
66794	degradation of pentoses	42.86	12 of 28
66794	3-chlorocatechol degradation	40	2 of 5
66794	3-phenylpropionate degradation	40	6 of 15
66794	gallate degradation	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	polyamine pathway	39.13	9 of 23
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	urea cycle	38.46	5 of 13
66794	carnitine metabolism	37.5	3 of 8
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	sphingosine metabolism	33.33	2 of 6
66794	methanogenesis from CO2	33.33	4 of 12
66794	pantothenate biosynthesis	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	ascorbate metabolism	31.82	7 of 22
66794	carotenoid biosynthesis	31.82	7 of 22
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	cholesterol biosynthesis	27.27	3 of 11
66794	cyclohexanol degradation	25	1 of 4
66794	alginate biosynthesis	25	1 of 4

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22675	sediment of a freshwater lake	Lake Mizugaki	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence LC062790 (>99% sequence identity) for Sulfurifustis variabilis subclade from Microbeatlas

Aquatic Samples	473
Soil Samples	791
Animal Samples	85
Plant Samples	47
Total Samples	1396

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22675	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM235541v1 assembly for Sulfurifustis variabilis skN76	complete	GCA_002355415	1675686.5	2775506818	1675686	93.23

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22675	Sulfurifustis variabilis gene for 16S ribosomal RNA, partial sequence	LC062790	1454		1675686

GC content

@ref	GC-content (mol%)	Method
22675	69	high performance liquid chromatography (HPLC)
22675	67.5	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Sulfurifustis variabilis skN76
NCBI: GCA_002355415

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.00	no
125439	motility	BacteriaNetⓘ	yes	64.30	no
125439	gram_stain	BacteriaNetⓘ	negative	96.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	93.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Sulfurifustis variabilis skN76
NCBI: GCA_002355415.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.75	no
125438	anaerobic	anaerobicⓘ	no	88.39	yes
125438	aerobic	aerobicⓘ	yes	50.61	yes
125438	spore-forming	spore-formingⓘ	no	87.87	no
125438	thermophilic	thermophileⓘ	no	92.04	no
125438	flagellated	motile2+ⓘ	no	54.27	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	The complete genome sequences of sulfur-oxidizing Gammaproteobacteria Sulfurifustis variabilis skN76(T) and Sulfuricaulis limicola HA5(T).	Umezawa K, Watanabe T, Miura A, Kojima H, Fukui M	Stand Genomic Sci	10.1186/s40793-016-0196-0	2016
Phylogeny	Sulfurifustis variabilis gen. nov., sp. nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov.	Kojima H, Shinohara A, Fukui M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000479	2015
Phylogeny	Sulfuricaulis limicola gen. nov., sp. nov., a sulfur oxidizer isolated from a lake.	Kojima H, Watanabe T, Fukui M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000709	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22675	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100313
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Sulfurifustis variabilis (DSM 100313, NBRC 110942, skN76)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002355415

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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