Name: Pseudomonas mohnii IPA-2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13138

Type strain:

Species: Pseudomonas mohnii

Strain Designation: IPA-2

Culture col. no.: DSM 18327, CIP 109614, CCUG 53115

Strain history: CIP <- 2007, CCUG <- 2006, B. Camara, D.H. Pieper, HZI, Braunschweig, Germany: strain IpA-2

NCBI tax ID(s): 395600 (species)

SI-ID 303228

CCUG 53115, DSM 18327, CIP 109614

Special Collections

Section

Pseudomonas mohnii IPA-2 is an obligate aerobe, Gram-negative bacterium that was isolated from sequencing batch reactor treating paper mill effluent, enrichment with isopimaric acid.

Gram-negative
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas mohnii**
	Full Scientific Name (LPSN)	Pseudomonas mohnii Cámara et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31953]	Gram stain	negative
[Ref.: #120999]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.5

[Ref.: #120999]	Cell shape	rod-shaped

[Ref.: #120999]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %90.972

[Ref.: #31953]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7506]

Culture medium

REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b)

[Ref.: #7506]

Culture medium growth

[Ref.: #7506]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7506]

Culture medium composition

expand / minimize

[Ref.: #37788]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37788]

Culture medium growth

[Ref.: #37788]

Culture medium composition

expand / minimize

[Ref.: #120999]

Culture medium

CIP Medium 72

[Ref.: #120999]

Culture medium growth

[Ref.: #120999]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #7506]			growth	30 °C
[Ref.: #37788]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #120999]	Oxygen tolerance	obligate aerobe
[Ref.: #69480]	Oxygen tolerance	aerobe Confidence in %94.957

[Ref.: #31953]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31953]		NaCl		growth	<5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31953]	4-hydroxy-L-proline ChEBI	+	carbon source
[Ref.: #31953]	alanine ChEBI	+	carbon source
[Ref.: #31953]	arabinose ChEBI	+	carbon source
[Ref.: #31953]	asparagine ChEBI	+	carbon source
[Ref.: #31953]	aspartate ChEBI	+	carbon source
[Ref.: #31953]	DL-carnitine ChEBI	+	carbon source
[Ref.: #31953]	formate ChEBI	+	carbon source
[Ref.: #31953]	galactose ChEBI	+	carbon source
[Ref.: #31953]	gamma-aminobutyric acid ChEBI	+	carbon source
[Ref.: #31953]	gluconate ChEBI	+	carbon source
[Ref.: #31953]	glucose ChEBI	+	carbon source
[Ref.: #31953]	glutamate ChEBI	+	carbon source
[Ref.: #31953]	glycerol ChEBI	+	carbon source
[Ref.: #31953]	lactate ChEBI	+	carbon source
[Ref.: #31953]	malonate ChEBI	+	carbon source
[Ref.: #31953]	mannitol ChEBI	+	carbon source
[Ref.: #31953]	mannose ChEBI	+	carbon source
[Ref.: #31953]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #120999]	nitrate ChEBI	-	reduction
[Ref.: #120999]	nitrite ChEBI	-	reduction
[Ref.: #31953]	phenylethylamine ChEBI	+	carbon source
[Ref.: #31953]	proline ChEBI	+	carbon source
[Ref.: #31953]	propionate ChEBI	+	carbon source
[Ref.: #31953]	putrescine ChEBI	+	carbon source
[Ref.: #31953]	quinate ChEBI	+	carbon source
[Ref.: #31953]	serine ChEBI	+	carbon source
[Ref.: #31953]	succinate ChEBI	+	carbon source
[Ref.: #31953]	urocanic acid ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120999]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120999]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31953]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120999]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #120999]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120999]	12000	-	+	+	-	-	+	+	-	+	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7506]	Sample type/isolated from	sequencing batch reactor treating paper mill effluent, enrichment with isopimaric acid
[Ref.: #7506]	Country	Canada
[Ref.: #7506]	Country ISO 3 Code	CAN
[Ref.: #7506]	Continent	North America

[Ref.: #120999]	Sample type/isolated from	Sequencing batch bioreactor, stationary enrichment
[Ref.: #120999]	Country	Germany
[Ref.: #120999]	Country ISO 3 Code	DEU
[Ref.: #120999]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Bioreactor	-
#Engineered	#Industrial	#Industrial production
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X96788 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	124
Soil Samples	197
Animal Samples	162
Plant Samples	153
Total Samples	636

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7506]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120999]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31953]	Pseudomonas mohnii partial 16S rRNA gene, type strain IpA-2T	AM293567	1459	GenBank ENA	395600 tax ID
[Ref.: #7506]	Pseudomonas sp. 16S rRNA gene, strain IpA-2	X96788	1492	GenBank ENA	306 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas mohnii DSM 18327	GCA_900105115	contig	GenBank ENA	395600 tax ID	*
[Ref.: #66792]	Pseudomonas mohnii strain DSM 18327	395600.4	wgs	PATRIC	395600 tax ID	*
[Ref.: #66792]	Pseudomonas mohnii DSM 18327	2667527224	draft	JGI IMG/M	395600 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas mohnii strain DSM 18327 PATRIC: 395600.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.5	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	98.359	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	84.939	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	94.957	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	98.986	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	90.972	no

Availability in culture collections External links

[Ref.: #7506]

Culture collection no.

DSM 18327, CIP 109614, CCUG 53115

[Ref.: #82346]

SI-ID 303228

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas reinekei sp. nov., Pseudomonas moorei sp. nov. and Pseudomonas mohnii sp. nov., novel species capable of degrading chlorosalicylates or isopimaric acid.	Camara B, Strompl C, Verbarg S, Sproer C, Pieper DH, Tindall BJ	Int J Syst Evol Microbiol	10.1099/ijs.0.64703-0	2007	*

References

#7506

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18327

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31953

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28209 (see below)

#37788 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82346

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID303228.1 )

#120999 Collection of Institut Pasteur ; Curators of the CIP; CIP 109614

#28209

IJSEM 923 2007 ( DOI 10.1099/ijs.0.64703-0 , PubMed 17473234 )

* These data were automatically processed and therefore are not curated

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Pseudomonas mohnii strain DSM 18327

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