Strain identifier

BacDive ID: 13138

Type strain: Yes

Species: Pseudomonas mohnii

Strain Designation: IPA-2

Strain history: CIP <- 2007, CCUG <- 2006, B. Camara, D.H. Pieper, HZI, Braunschweig, Germany: strain IpA-2

NCBI tax ID(s): 395600 (species)

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General

@ref: 7506

BacDive-ID: 13138

DSM-Number: 18327

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative

description: Pseudomonas mohnii IPA-2 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from sequencing batch reactor treating paper mill effluent, enrichment with isopimaric acid.

NCBI tax id

  • NCBI tax id: 395600
  • Matching level: species

strain history

@refhistory
7506<- D. Pieper, GBF Braunschweig; IPA-2 <- A. E. J. Wilson, Univ. Vancouver
120999CIP <- 2007, CCUG <- 2006, B. Camara, D.H. Pieper, HZI, Braunschweig, Germany: strain IpA-2

doi: 10.13145/bacdive13138.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas mohnii
  • full scientific name: Pseudomonas mohnii Cámara et al. 2007

@ref: 7506

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas mohnii

full scientific name: Pseudomonas mohnii Cámara et al. 2007

strain designation: IPA-2

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
31953negative
120999negativerod-shapedyes

pigmentation

  • @ref: 31953
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7506REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b)yeshttps://mediadive.dsmz.de/medium/535bName: REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b) Composition: Trypticase soy broth 30.0 g/l Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l K2HPO4 2.5 g/l D(+)-Glucose 2.5 g/l Distilled water
37788MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120999CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
7506positivegrowth30mesophilic
37788positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120999
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 31953
  • spore formation: no

halophily

  • @ref: 31953
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3195316449alanine+carbon source
3195322599arabinose+carbon source
3195322653asparagine+carbon source
3195335391aspartate+carbon source
3195317126DL-carnitine+carbon source
3195315740formate+carbon source
3195328260galactose+carbon source
3195316865gamma-aminobutyric acid+carbon source
3195324265gluconate+carbon source
3195317234glucose+carbon source
3195329987glutamate+carbon source
3195317754glycerol+carbon source
31953182404-hydroxy-L-proline+carbon source
3195324996lactate+carbon source
3195315792malonate+carbon source
3195329864mannitol+carbon source
3195337684mannose+carbon source
3195351850methyl pyruvate+carbon source
3195350048phenylethylamine+carbon source
3195326271proline+carbon source
3195317272propionate+carbon source
3195317148putrescine+carbon source
3195326490quinate+carbon source
3195317822serine+carbon source
3195330031succinate+carbon source
3195327248urocanic acid+carbon source
12099917632nitrate-reduction
12099916301nitrite-reduction

metabolite production

  • @ref: 120999
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31953cytochrome oxidase+1.9.3.1
120999oxidase+
120999catalase+1.11.1.6
120999urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120999-++--++-+-++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7506sequencing batch reactor treating paper mill effluent, enrichment with isopimaric acidCanadaCANNorth America
120999Sequencing batch bioreactor, stationary enrichmentGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Engineered#Industrial#Industrial production
#Engineered#Waste#Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_444.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_444&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: X96788
  • Sequence Identity:
  • Total samples: 636
  • soil counts: 197
  • aquatic counts: 124
  • animal counts: 162
  • plant counts: 153

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
75061Risk group (German classification)
1209991Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
31953Pseudomonas mohnii partial 16S rRNA gene, type strain IpA-2TAM2935671459nuccore395600
7506Pseudomonas sp. 16S rRNA gene, strain IpA-2X967881492ena306

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas mohnii DSM 18327GCA_900105115contigncbi395600
66792Pseudomonas mohnii strain DSM 18327395600.4wgspatric395600
66792Pseudomonas mohnii DSM 183272667527224draftimg395600

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.198no
anaerobicno98.019no
halophileno84.358no
spore-formingno96.059yes
glucose-utilyes92.801no
aerobicyes93.129no
motileyes91.982no
flagellatedyes82.714no
thermophileno99.841yes
glucose-fermentno91.752no

External links

@ref: 7506

culture collection no.: DSM 18327, CIP 109614, CCUG 53115

straininfo link

  • @ref: 82346
  • straininfo: 303228

literature

  • topic: Phylogeny
  • Pubmed-ID: 17473234
  • title: Pseudomonas reinekei sp. nov., Pseudomonas moorei sp. nov. and Pseudomonas mohnii sp. nov., novel species capable of degrading chlorosalicylates or isopimaric acid.
  • authors: Camara B, Strompl C, Verbarg S, Sproer C, Pieper DH, Tindall BJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64703-0
  • year: 2007
  • mesh: Bacterial Typing Techniques, Carboxylic Acids/*metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phenanthrenes/*metabolism, Phylogeny, Pseudomonas/chemistry/*classification/isolation & purification/*physiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Salicylates/*metabolism, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7506Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18327)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18327
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31953Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2820928776041
37788Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7377
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82346Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID303228.1StrainInfo: A central database for resolving microbial strain identifiers
120999Curators of the CIPCollection of Institut Pasteur (CIP 109614)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109614