Name: Pseudomonas reinekei MT1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13139

Type strain:

Species: Pseudomonas reinekei

Strain Designation: MT1

Culture col. no.: DSM 18361, CCM 7743, CCUG 53116, CIP 109621

Strain history: CIP <- 2007, CCUG <- 2006, B. Camara & D.H. Pieper, HZI, Braunschweig, Germany: strain MT1

NCBI tax ID(s): 395598 (species)

SI-ID 354058

CCUG 53116, DSM 18361, CIP 109621

Section

Pseudomonas reinekei MT1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from aerobic zone of Elbe sediment enriched with 4-chlorosalicylate.

Gram-negative
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas reinekei**
	Full Scientific Name (LPSN)	Pseudomonas reinekei Cámara et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31953]	Gram stain	negative
[Ref.: #122152]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #122152]	Cell shape	rod-shaped

[Ref.: #122152]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.546

[Ref.: #31953]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7436]

Culture medium

MINERAL MEDIUM PH 7.25 (DSMZ Medium 465)

[Ref.: #7436]

Culture medium growth

[Ref.: #7436]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7436]

Culture medium composition

expand / minimize

[Ref.: #7436]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #7436]

Culture medium growth

[Ref.: #7436]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7436]

Culture medium composition

expand / minimize

[Ref.: #37791]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37791]

Culture medium growth

[Ref.: #37791]

Culture medium composition

expand / minimize

[Ref.: #122152]

Culture medium

CIP Medium 72

[Ref.: #122152]

Culture medium growth

[Ref.: #122152]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #7436]			growth	30 °C
[Ref.: #37791]			growth	30 °C

[Ref.: #7436]	Temperature range	mesophilic
[Ref.: #37791]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31953]	Oxygen tolerance	aerobe
[Ref.: #122152]	Oxygen tolerance	obligate aerobe

[Ref.: #31953]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.989

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31953]		NaCl		growth	<5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31953]	acetate ChEBI	+	carbon source
[Ref.: #31953]	alanine ChEBI	+	carbon source
[Ref.: #31953]	arabinose ChEBI	+	carbon source
[Ref.: #31953]	asparagine ChEBI	+	carbon source
[Ref.: #31953]	aspartate ChEBI	+	carbon source
[Ref.: #31953]	citrate ChEBI	+	carbon source
[Ref.: #31953]	DL-carnitine ChEBI	+	carbon source
[Ref.: #31953]	ethanolamine ChEBI	+	carbon source
[Ref.: #31953]	formate ChEBI	+	carbon source
[Ref.: #31953]	galactose ChEBI	+	carbon source
[Ref.: #31953]	galacturonate ChEBI	+	carbon source
[Ref.: #31953]	gluconate ChEBI	+	carbon source
[Ref.: #31953]	glucose ChEBI	+	carbon source
[Ref.: #31953]	glutamate ChEBI	+	carbon source
[Ref.: #31953]	glycerol ChEBI	+	carbon source
[Ref.: #31953]	L-alaninamide ChEBI	+	carbon source
[Ref.: #31953]	L-arabitol ChEBI	+	carbon source
[Ref.: #31953]	lactate ChEBI	+	carbon source
[Ref.: #31953]	malonate ChEBI	+	carbon source
[Ref.: #31953]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #122152]	nitrate ChEBI	-	reduction
[Ref.: #122152]	nitrite ChEBI	-	reduction
[Ref.: #31953]	phenylethylamine ChEBI	+	carbon source
[Ref.: #31953]	proline ChEBI	+	carbon source
[Ref.: #31953]	propionate ChEBI	+	carbon source
[Ref.: #31953]	quinate ChEBI	+	carbon source
[Ref.: #31953]	serine ChEBI	+	carbon source
[Ref.: #31953]	succinate ChEBI	+	carbon source
[Ref.: #31953]	urocanic acid ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #122152]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122152]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31953]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122152]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122152]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122152]	12007	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7436]	Sample type/isolated from	aerobic zone of Elbe sediment enriched with 4-chlorosalicylate
[Ref.: #7436]	Geographic location (country and/or sea, region)	Sachsen Anhalt, Bitterfeld
[Ref.: #7436]	Country	Germany
[Ref.: #7436]	Country ISO 3 Code	DEU
[Ref.: #7436]	Continent	Europe

[Ref.: #122152]	Sample type/isolated from	Continuous enrichment on 4-chlorosalicylate
[Ref.: #122152]	Geographic location (country and/or sea, region)	Bitterfeld, Elbe River
[Ref.: #122152]	Country	Germany
[Ref.: #122152]	Country ISO 3 Code	DEU
[Ref.: #122152]	Continent	Europe
[Ref.: #122152]	Isolation date	1993
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM293565 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	448
Soil Samples	833
Animal Samples	521
Plant Samples	662
Total Samples	2464

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7436]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122152]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31953]	Pseudomonas reinekei partial 16S rRNA gene, type strain MT1T	AM293565	1492	GenBank	395598 tax ID
[Ref.: #20218]	Pseudomonas reinekei strain MT1 16S ribosomal RNA gene, partial sequence	JQ178342	421	ENA	395598 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas reinekei MT1	GCA_001945365	contig	GenBank	395598 tax ID	*
[Ref.: #66792]	Pseudomonas reinekei CCUG 53116	GCA_008801455	contig	GenBank	395598 tax ID	*
[Ref.: #66792]	Pseudomonas reinekei strain CCUG 53116	395598.13	wgs	PATRIC	395598 tax ID	*
[Ref.: #66792]	Pseudomonas reinekei DSM 18361	2639762506	draft	JGI IMG/M	395598 tax ID	*
[Ref.: #66792]	Pseudomonas reinekei MT1	2582581646	draft	JGI IMG/M	395598 tax ID	*
[Ref.: #66792]	Pseudomonas reinekei DSM 18361	GCA_900104125	chromosome	GenBank	395598 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudomonas reinekei strain CCUG 53116 PATRIC: 395598.4
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas reinekei DSM 18361 NCBI: GCA_900104125.1
[Ref.: #69480]	motile	yes	92.134	no
[Ref.: #69480]	flagellated	yes	86.289	no
[Ref.: #69480]	gram-positive	no	98.112	yes
[Ref.: #69480]	anaerobic	no	98.434	yes
[Ref.: #69480]	aerobic	yes	93.137	yes
[Ref.: #69480]	halophile	no	86.333	no
[Ref.: #69480]	spore-forming	no	96.128	no
[Ref.: #69480]	glucose-ferment	no	90.611	no
[Ref.: #69480]	thermophile	no	99.842	yes
[Ref.: #69480]	glucose-util	yes	93.482	yes

Availability in culture collections External links

[Ref.: #7436]

Culture collection no.

DSM 18361, CCM 7743, CCUG 53116, CIP 109621

[Ref.: #82347]

SI-ID 354058

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas reinekei sp. nov., Pseudomonas moorei sp. nov. and Pseudomonas mohnii sp. nov., novel species capable of degrading chlorosalicylates or isopimaric acid.	Camara B, Strompl C, Verbarg S, Sproer C, Pieper DH, Tindall BJ	Int J Syst Evol Microbiol	10.1099/ijs.0.64703-0	2007	*

References

#7436

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18361

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31953

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28209 (see below)

#37791 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82347

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID354058.1 )

#122152 Collection of Institut Pasteur ; Curators of the CIP; CIP 109621

#28209

IJSEM 923 2007 ( DOI 10.1099/ijs.0.64703-0 , PubMed 17473234 )

* These data were automatically processed and therefore are not curated

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Pseudomonas reinekei strain CCUG 53116

Pseudomonas reinekei DSM 18361

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