Pseudomonas viridiflava (DSM 11124, ATCC 13223, DSM 6694)

Name: Pseudomonas viridiflava (DSM 11124, ATCC 13223, DSM 6694)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13016

Type strain

Strain Designation PV6

Variant Isotype of BacDive ID 24401

Culture col. no. DSM 11124 ATCC 13223 DSM 6694 NCPPB 635 PV6 4 more

NCBI tax ID(s) 33069

Special Collections DSMZ CCUG CIP

History <- ATCC <- W. H. Burkholder; PV6 CIP <- 2000, CCUG <- J.M. Young, ICMP, Auckland, New Zealand <- D.W. Dye <- NCPPB <- W.H. Burkholder

Links

Pseudomonas viridiflava PV6 is an obligate aerobe, mesophilic, Gram-negative plant pathogen that was isolated from dwarf or runner bean.

Gram-negative motile rod-shaped obligate aerobe mesophilic plant pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 6872

LMG 2352,ATCC 13223,CECT 458,NCPPB 602,NCPPB 635,NRRL B-895,PDDCC 2848,CCUG 23371,CIP 106699,DSM 11124,CFBP 2107,CCM 4962,ICMP 2847,ICMP 2848,KACC 10391

@ref 20215

Last LPSN update 2026-02-09 11:21:00

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas viridiflava

Full scientific name Pseudomonas viridiflava (Burkholder 1930) Dowson 1939 (Approved Lists 1980)

Synonyms (1)

"Phytomonas viridiflava"

BacDive ID	Other strains from **Pseudomonas viridiflava** (4)	Type strain
24401	P. viridiflava 625, DSM 6694, ATCC 13223 (type strain)
130571	P. viridiflava UASWS0038, UASWS38, DSM 28655
144183	P. viridiflava CCUG 23370, ICMP 7556a, NCPPB 2012, LMG 5399
157487	P. viridiflava IMI 349403

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123865	negative	rod-shaped
125438	negative		99.5
125439	negative		99.6

Pigmentation

@ref	Production	Name
123865		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4216	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
32999	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
4216	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
4216	PSEUDOMONAS AGAR F (DSMZ Medium 907)	Medium recipe at MediaDive	Name: PSEUDOMONAS AGAR F (DSMZ Medium 907) Composition: Agar 15.0 g/l Glycerol 10.0 g/l Proteose peptone 10.0 g/l Tryptone 10.0 g/l MgSO4 1.5 g/l K2HPO4 1.5 g/l Distilled water
123865	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4216	positive	growth	28	mesophilic
4216	positive	growth	25
32999	positive	growth	25	mesophilic
123865	positive	growth	5-30
123865	negative	growth	37
123865	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
123865	obligate aerobe
125438	aerobe	93
125439	obligate aerobe	98.5

Spore formation

@ref	Spore formation	Confidence
125439		99.3

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123865	NaCl	positive	growth	0-4 %
123865	NaCl		growth	6 %
123865	NaCl		growth	8 %
123865	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
123865	16947 ChEBI	citrate	+	carbon source
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
123865	4853 ChEBI	esculin	+	hydrolysis
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
123865	17632 ChEBI	nitrate	-	reduction
123865	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
123865	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
123865	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
123865	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123865	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123865	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
123865	beta-galactosidase	-	3.2.1.23
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123865	caseinase	+	3.4.21.50
123865	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
123865	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123865	gelatinase	-
123865	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123865	lipase	-
68382	lipase (C 14)	-		from API zym
123865	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123865	ornithine decarboxylase	-	4.1.1.17
123865	oxidase	-
123865	protease	-
68382	trypsin	+	3.4.21.4	from API zym
123865	tryptophan deaminase	-
123865	tween esterase	-
123865	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123865	-	+	+	+	-	+	-	-	+	-	+	+	-	-	-	-	+	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
4216	-	-	-	-	-	+	+/-	-	+	+	-	+	-	-	+	+	-	+	+	-	+

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
123865	not determinedn.d.	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	+	-	-	-	-	+	+	+	-	-	+	-	-	+	+	-	-	+	+	-	+	+	+	+	+	+	-	+	+	+	-	-	-	-	+	-	+	+	+	-	-	-	-	-	+	+	-	+	-	+	-	+	-	+	+	+	+	+	-	-	+	-	+	+	+	+	+	+	+	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Shrub (Scrub)

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Isolation date
4216	dwarf or runner bean	Switzerland	CHE	Europe
123865	Dwarf or runner bean	Switzerland	CHE	Europe	1927

Taxonmaps

69479

Global distribution of 16S sequence Z76671 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	147
Soil Samples	184
Animal Samples	195
Plant Samples	210
Total Samples	736

Interaction and safety

Risk assessment

@ref	Pathogenicity plant	Biosafety level	Biosafety level comment
4216		1	Risk group (German classification) According to TRBA 466
123865		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM130595v1 assembly for Pseudomonas viridiflava DSM 6694	contig	GCA_001305955	33069.8	2639762757	33069	41.93

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Pseudomonas viridiflava strain ATCC 13223 16S ribosomal RNA gene, partial sequence	AF094751	1461	33069
20218	Pseudomonas viridiflava culture-collection CFBP:2107 16S ribosomal RNA gene, partial sequence	HM190229	1442	33069
20218	P.viridiflava 16S rRNA gene	Z76671	1492	33069

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas viridiflava DSM 6694
NCBI: GCA_001305955

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.30	no
125439	motility	BacteriaNetⓘ	yes	88.70	no
125439	gram_stain	BacteriaNetⓘ	negative	99.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas viridiflava strain DSM 6694
PATRIC: 33069.8

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	no
125438	anaerobic	anaerobicⓘ	no	99.32	no
125438	spore-forming	spore-formingⓘ	no	80.46	no
125438	aerobic	aerobicⓘ	yes	93.00	yes
125438	thermophilic	thermophileⓘ	no	98.50	no
125438	flagellated	motile2+ⓘ	yes	88.30	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Isolation and partial characterization of antagonistic peptides produced by Paenibacillus sp. strain B2 isolated from the sorghum mycorrhizosphere.	Selim S, Negrel J, Govaerts C, Gianinazzi S, van Tuinen D.	Appl Environ Microbiol	10.1128/aem.71.11.6501-6507.2005	2005
	First Report of Pseudomonas viridiflava Causing Bacterial Blight on Globe Artichoke in Turkey	Sanver U, Pazarlar S, Cetinkaya N, Ozaktan H.	Plant Dis	10.1094/pdis-03-19-0483-pdn	2019
Enzymology	Identification of an emergent and atypical Pseudomonas viridiflava lineage causing bacteriosis in plants of agronomic importance in a Spanish region.	Gonzalez AJ, Rodicio MR, Mendoza MC.	Appl Environ Microbiol	10.1128/aem.69.5.2936-2941.2003	2003
Enzymology	Sensitive detection of Xanthomonas oryzae Pathovars oryzae and oryzicola by loop-mediated isothermal amplification.	Lang JM, Langlois P, Nguyen MH, Triplett LR, Purdie L, Holton TA, Djikeng A, Vera Cruz CM, Verdier V, Leach JE.	Appl Environ Microbiol	10.1128/aem.00274-14	2014
Metabolism	Comparative genome analysis of the vineyard weed endophyte Pseudomonas viridiflava CDRTc14 showing selective herbicidal activity.	Samad A, Antonielli L, Sessitsch A, Compant S, Trognitz F.	Sci Rep	10.1038/s41598-017-16495-y	2017
	First Report of Pseudomonas viridiflava as the Causal Agent of Bacterial Leaf Spot of Mexican Heather in California	Albu S, Lai M, Woods PW, Kumagai LB.	Plant Dis	10.1094/pdis-03-18-0405-pdn	2018
	Calendula officinalis: A New Natural Host of Pseudomonas viridiflava in Italy.	Moretti C, Fakhr R, Buonaurio R.	Plant Dis	10.1094/pdis-08-11-0691	2012
Metabolism	LlpB represents a second subclass of lectin-like bacteriocins.	Ghequire MGK, De Mot R.	Microb Biotechnol	10.1111/1751-7915.13373	2019
	First Report of Leaf Necrosis Caused by Pseudomonas viridiflava on Melon Seedlings in Italy.	Moretti C, Sequino S, Buonaurio R.	Plant Dis	10.1094/pd-89-0109a	2005
Metabolism	Production and comparison of peptide siderophores from strains of distantly related pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352.	Bultreys A, Gheysen I.	Appl Environ Microbiol	10.1128/aem.66.1.325-331.2000	2000
	Biological and molecular detection of toxic lipodepsipeptide-producing pseudomonas syringae strains and PCR identification in plants	Bultreys A, Gheysen I.	Appl Environ Microbiol	10.1128/aem.65.5.1904-1909.1999	1999
Metabolism	Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates.	Ghequire MG, Dillen Y, Lambrichts I, Proost P, Wattiez R, De Mot R.	Genome Biol Evol	10.1093/gbe/evv184	2015
Enzymology	Plant lectin-like bacteriocin from a rhizosphere-colonizing Pseudomonas isolate.	Parret AH, Schoofs G, Proost P, De Mot R.	J Bacteriol	10.1128/jb.185.3.897-908.2003	2003
Phylogeny	Characterization of fluorescent and nonfluorescent peptide siderophores produced by Pseudomonas syringae strains and their potential use in strain identification.	Bultreys A, Gheysen I, Maraite H, de Hoffmann E.	Appl Environ Microbiol	10.1128/aem.67.4.1718-1727.2001	2001
Phylogeny	Pseudomonas alliivorans sp. nov., a plant-pathogenic bacterium isolated from onion foliage in Georgia, USA.	Zhao M, Tyson C, Chen HC, Paudel S, Gitaitis R, Kvitko B, Dutta B	Syst Appl Microbiol	10.1016/j.syapm.2021.126278	2021
Phylogeny	Pseudomonas floridensis sp. nov., a bacterial pathogen isolated from tomato.	Timilsina S, Minsavage GV, Preston J, Newberry EA, Paret ML, Goss EM, Jones JB, Vallad GE	Int J Syst Evol Microbiol	10.1099/ijsem.0.002445	2017

References

#4216	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11124
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#32999	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123865	Collection of Institut Pasteur ; Curators of the CIP; CIP 106699
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas viridiflava (DSM 11124, ATCC 13223, DSM 6694)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001305955

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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