Alkalihalobacterium alkalinitrilicum (DSM 22532, NCCB 100120, UNIQEM U240)

Name: Alkalihalobacterium alkalinitrilicum (DSM 22532, NCCB 100120, UNIQEM U240)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1283

Type strain

Strain Designation ANL-iso4

Culture col. no. DSM 22532 NCCB 100120 UNIQEM U240 ANL-iso4

NCBI tax ID(s) 427920

Special Collections DSMZ

History <- D. Y. Sorokin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; ANL-iso4

Links

Alkalihalobacterium alkalinitrilicum ANL-iso4 is a bacterium that was isolated from soda solonchak soils.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 405589

NCCB 100120,DSM 22532

@ref 20215

Last LPSN update 2026-02-09 11:18:04

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Alkalihalobacterium

Species Alkalihalobacterium alkalinitrilicum

Full scientific name Alkalihalobacterium alkalinitrilicum (Sorokin et al. 2009) Joshi et al. 2022

Synonyms (2)

Alkalihalobacillus alkalinitrilicus
Bacillus alkalinitrilicus

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16381	NITRILIRUPTOR ALKALIPHILUS MEDIUM (DSMZ Medium 1105)		Medium recipe at MediaDive	Name: NITRILIRUPTOR ALKALIPHILUS MEDIUM (DSMZ Medium 1105) Composition: Na2CO3 22.0 g/l NaHCO3 8.0 g/l NaCl 6.0 g/l Isobutyramide 0.8712 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 0.0001 g/l Vitamin B12 0.0001 g/l MnCl2 3e-05 g/l NiCl2 x 2 H2O 2e-05 g/l Na2MoO4 x 2 H2O 2e-05 g/l None 2e-05 g/l CuCl2 1e-05 g/l Na2S2O3 x 5 H2O Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
16381	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	facultative anaerobe	97.9

Spore formation

@ref	Spore formation	Confidence
125439		94.9
125438		90.069

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Condition	#Alkaline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
16381	soda solonchak soils	southwestern Siberia, Altai region, Kulunda Steppe	Russia	RUS	Asia

Taxonmaps

69479

Global distribution of 16S sequence EF422411 (>99% sequence identity) for Alkalihalobacillus alkalinitrilicus subclade from Microbeatlas

Aquatic Samples	28
Soil Samples	59
Animal Samples	14
Plant Samples	4
Total Samples	105

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16381	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM201960v1 assembly for Alkalihalobacterium alkalinitrilicum DSM 22532	scaffold	GCA_002019605	427920.3	8022871258	427920	71.11

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16381	Bacillus alkalinitrilicus strain ANL-iso4 16S ribosomal RNA gene, partial sequence	EF422411	1472		427920

GC content

@ref	GC-content (mol%)	Method
16381	35.1	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Alkalihalobacterium alkalinitrilicum DSM 22532
NCBI: GCA_002019605

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	94.90	no
125439	motility	BacteriaNetⓘ	yes	84.50	no
125439	gram_stain	BacteriaNetⓘ	variable	61.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	97.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alkalihalobacillus alkalinitrilicus DSM 22532
NCBI: GCA_002019605.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	72.39	no
125438	anaerobic	anaerobicⓘ	no	86.15	no
125438	aerobic	aerobicⓘ	yes	69.86	no
125438	spore-forming	spore-formingⓘ	yes	90.07	no
125438	thermophilic	thermophileⓘ	no	88.52	no
125438	flagellated	motile2+ⓘ	yes	78.47	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alkalihalobacterium elongatum gen. nov. sp. nov.: An Antibiotic-Producing Bacterium Isolated From Lonar Lake and Reclassification of the Genus Alkalihalobacillus Into Seven Novel Genera.	Joshi A, Thite S, Karodi P, Joseph N, Lodha T	Front Microbiol	10.3389/fmicb.2021.722369	2021
Phylogeny	Lottiidibacillus patelloidae gen. nov., sp. nov., isolated from the intestinal tract of a marine limpet and reclassification of Bacillus taeanensis as Maribacillus taeanensis gen. nov., comb. nov.	Liu R, Huang Z, Dong C, Shao Z	Antonie Van Leeuwenhoek	10.1007/s10482-018-01213-z	2019
Phylogeny	Desertibacillus haloalkaliphilus gen. nov., sp. nov., isolated from a saline desert.	Bhatt HB, Azmatunnisa Begum M, Chintalapati S, Chintalapati VR, Singh SP	Int J Syst Evol Microbiol	10.1099/ijsem.0.002310	2017
Phylogeny	Utilization of aliphatic nitriles under haloalkaline conditions by Bacillus alkalinitrilicus sp. nov. isolated from soda solonchak soil.	Sorokin DY, van Pelt S, Tourova TP	FEMS Microbiol Lett	10.1111/j.1574-6968.2008.01353.x	2008

References

#16381	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22532
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alkalihalobacterium alkalinitrilicum (DSM 22532, NCCB 100120, UNIQEM U240)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002019605

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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