Strain identifier
BacDive ID: 1283
Type strain: 
Species: Alkalihalobacterium alkalinitrilicum
Strain Designation: ANL-iso4
Strain history: <- D. Y. Sorokin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; ANL-iso4
NCBI tax ID(s): 427920 (species)
version 8.1 (current version)
General
@ref: 16381
BacDive-ID: 1283
DSM-Number: 22532
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, motile
description: Alkalihalobacterium alkalinitrilicum ANL-iso4 is a spore-forming, mesophilic, motile bacterium that was isolated from soda solonchak soils.
NCBI tax id
- NCBI tax id: 427920
- Matching level: species
strain history
- @ref: 16381
- history: <- D. Y. Sorokin, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; ANL-iso4
doi: 10.13145/bacdive1283.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Alkalihalobacterium
- species: Alkalihalobacterium alkalinitrilicum
- full scientific name: Alkalihalobacterium alkalinitrilicum (Sorokin et al. 2009) Joshi et al. 2022
synonyms
@ref synonym 20215 Alkalihalobacillus alkalinitrilicus 20215 Bacillus alkalinitrilicus
@ref: 16381
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Caryophanales
family: Bacillaceae
genus: Alkalihalobacterium
species: Alkalihalobacterium alkalinitrilicum
full scientific name: Alkalihalobacterium alkalinitrilicum (Sorokin et al. 2009) Joshi et al. 2022
strain designation: ANL-iso4
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 91.833 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 16381
- name: NITRILIRUPTOR ALKALIPHILUS MEDIUM (DSMZ Medium 1105)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1105
- composition: Name: NITRILIRUPTOR ALKALIPHILUS MEDIUM (DSMZ Medium 1105) Composition: Na2CO3 22.0 g/l NaHCO3 8.0 g/l NaCl 6.0 g/l Isobutyramide 0.8712 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 0.0001 g/l Vitamin B12 0.0001 g/l MnCl2 3e-05 g/l NiCl2 x 2 H2O 2e-05 g/l Na2MoO4 x 2 H2O 2e-05 g/l None 2e-05 g/l CuCl2 1e-05 g/l Na2S2O3 x 5 H2O Distilled water
culture temp
- @ref: 16381
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 16381
- sample type: soda solonchak soils
- geographic location: southwestern Siberia, Altai region, Kulunda Steppe
- country: Russia
- origin.country: RUS
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Condition | #Alkaline |
taxonmaps
- @ref: 69479
- File name: preview.99_1890.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1063;97_1241;98_1489;99_1890&stattab=map
- Last taxonomy: Alkalihalobacillus alkalinitrilicus subclade
- 16S sequence: EF422411
- Sequence Identity:
- Total samples: 105
- soil counts: 59
- aquatic counts: 28
- animal counts: 14
- plant counts: 4
Safety information
risk assessment
- @ref: 16381
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16381
- description: Bacillus alkalinitrilicus strain ANL-iso4 16S ribosomal RNA gene, partial sequence
- accession: EF422411
- length: 1472
- database: ena
- NCBI tax ID: 427920
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Alkalihalobacterium alkalinitrilicum DSM 22532 | GCA_002019605 | scaffold | ncbi | 427920 |
| 66792 | Bacillus alkalinitrilicus strain DSM 22532 | 427920.3 | wgs | patric | 427920 |
GC content
- @ref: 16381
- GC-content: 35.1
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | yes | 72.293 | no |
| gram-positive | yes | 87.053 | no |
| anaerobic | no | 95.089 | no |
| aerobic | yes | 83.82 | no |
| halophile | yes | 57.355 | no |
| spore-forming | yes | 96.709 | no |
| glucose-util | yes | 81.629 | no |
| thermophile | no | 99.02 | yes |
| glucose-ferment | no | 92.103 | no |
| motile | yes | 86.827 | no |
External links
@ref: 16381
culture collection no.: DSM 22532, NCCB 100120, UNIQEM U240
straininfo link
- @ref: 70937
- straininfo: 405589
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 18801047 | Utilization of aliphatic nitriles under haloalkaline conditions by Bacillus alkalinitrilicus sp. nov. isolated from soda solonchak soil. | Sorokin DY, van Pelt S, Tourova TP | FEMS Microbiol Lett | 10.1111/j.1574-6968.2008.01353.x | 2008 | Bacillus/*classification/genetics/isolation & purification/physiology, Hydrogen-Ion Concentration, Nitriles/*metabolism, Phenotype, Phylogeny, Russia, *Sodium Bicarbonate, Sodium Chloride/*pharmacology, *Soil Microbiology, Species Specificity, Spores, Bacterial/physiology | Phenotype |
| Phylogeny | 28920841 | Desertibacillus haloalkaliphilus gen. nov., sp. nov., isolated from a saline desert. | Bhatt HB, Azmatunnisa Begum M, Chintalapati S, Chintalapati VR, Singh SP | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002310 | 2017 | Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Phylogeny | 30600415 | Lottiidibacillus patelloidae gen. nov., sp. nov., isolated from the intestinal tract of a marine limpet and reclassification of Bacillus taeanensis as Maribacillus taeanensis gen. nov., comb. nov. | Liu R, Huang Z, Dong C, Shao Z | Antonie Van Leeuwenhoek | 10.1007/s10482-018-01213-z | 2019 | Animals, Bacillaceae/*classification/genetics/isolation & purification/metabolism, Bacillus/*classification/genetics/isolation & purification/metabolism, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Gastropoda/*microbiology, Intestines/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics | Metabolism |
| Phylogeny | 34707580 | Alkalihalobacterium elongatum gen. nov. sp. nov.: An Antibiotic-Producing Bacterium Isolated From Lonar Lake and Reclassification of the Genus Alkalihalobacillus Into Seven Novel Genera. | Joshi A, Thite S, Karodi P, Joseph N, Lodha T | Front Microbiol | 10.3389/fmicb.2021.722369 | 2021 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 16381 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22532) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22532 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 70937 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID405589.1 | StrainInfo: A central database for resolving microbial strain identifiers |