Name: Azotobacter vinelandii 3a
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12753

Type strain:

Species: Azotobacter vinelandii

Strain Designation: 3a, Johnstone 3a

Culture col. no.: DSM 87, ATCC 12837, NCIB 8789, CIP 104399, IFO 13581, NCIMB 8789

Strain history: CIP <- 1995, IFO <- 1973, Y. Einaga, Fac. Sci., Osaka Univ., Japan <- ATCC <- D.B. Johnstone: strain 3a

NCBI tax ID(s): 354 (species)

SI-ID 1083

LMG 3877, ATCC 12837, DSM 87, LMG 3882, NCIB 8789, CIP 104399, IFO 13581, NCIMB 8789, KCTC 2426, NBRC 13581, BCRC 15760, CCRC 15760, KACC 11120, NCAIM B.01796, NCIM 2821

Other strains from Azotobacter vinelandii

A. vinelandii 16, DSM 2289, ATCC 478, VKM B-1617, ... (type strain)
A. vinelandii 567, DSM 279, CCUG 51007
A. vinelandii 73, DSM 332
A. vinelandii OP, DSM 366, ATCC 13705
A. vinelandii Kg 2, DSM 382
A. vinelandii 1610, DSM 389, IMG 1610
A. vinelandii W2, DSM 390
A. vinelandii 5R, DSM 395
A. vinelandii DSM 399, RH Rh6, RH 6
A. vinelandii DSM 576, NCIB 9068
A. vinelandii 135, DSM 720, ATCC 17962, BKM B-547, ...
A. vinelandii VI, DSM 2290, ATCC 7496
A. vinelandii 1484, DSM 85, IMG 1484
A. vinelandii 1484, Claus 1484T, DSM 86
A. vinelandii 93-541b, DSM 13529
A. vinelandii JCM 20040, ATCC 9046, CECT 9203, ...

Section

Azotobacter vinelandii 3a is a mesophilic, Gram-negative, motile bacterium that was isolated from field soil.

Gram-negative
motile
rod-shaped
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Azotobacter*
	Species	**Azotobacter vinelandii**
	Full Scientific Name (LPSN)	Azotobacter vinelandii Lipman 1903 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #123846]	Gram stain	negative

[Ref.: #123846]	Cell shape	rod-shaped

[Ref.: #123846]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #36537]

Culture medium

MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii

[Ref.: #36537]

Culture medium growth

[Ref.: #36537]

Culture medium composition

expand / minimize

[Ref.: #2063]

Culture medium

DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441)

[Ref.: #2063]

Culture medium growth

[Ref.: #2063]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2063]

Culture medium composition

expand / minimize

[Ref.: #2063]

Culture medium

AZOTOBACTER MEDIUM (DSMZ Medium 3)

[Ref.: #2063]

Culture medium growth

[Ref.: #2063]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2063]

Culture medium composition

expand / minimize

[Ref.: #123846]

Culture medium

CIP Medium 1

[Ref.: #123846]

Culture medium growth

[Ref.: #123846]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2063]		growth	30 °C
[Ref.: #36537]		growth	30 °C
[Ref.: #123846]		growth	30-41 °C
[Ref.: #123846]	no	growth	10 °C

[Ref.: #2063]	Temperature range	mesophilic
[Ref.: #36537]	Temperature range	mesophilic
[Ref.: #123846]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123846]	citrate ChEBI	-	carbon source
[Ref.: #123846]	nitrate ChEBI	+	reduction
[Ref.: #123846]	nitrate ChEBI	-	respiration
[Ref.: #123846]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #123846]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #123846]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #123846]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #123846]	amylase	-
[Ref.: #123846]	beta-galactosidase	-	3.2.1.23
[Ref.: #123846]	caseinase	-	3.4.21.50
[Ref.: #123846]	catalase	+	1.11.1.6
[Ref.: #123846]	DNase	-
[Ref.: #123846]	gelatinase	-
[Ref.: #123846]	lysine decarboxylase	-	4.1.1.18
[Ref.: #123846]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123846]	oxidase	+
[Ref.: #123846]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #123846]	651	not determinedn.d.	+	+	+	-	-	+	+	+	+	-	-	-	-	-	+	-	-	-	+	-	-	-	+	+	-	+	+	-	+	-	-	+	+	+	-	+	+	-	-	-	+	-	-	-	+	+	-	+	+	+	+	-	-	+	-	-	+	+	-	-	+	-	+	+	+	-	-	+	+	-	-	-	-	-	-	+	+	+	-	+	+	+	+	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2063]	Sample type/isolated from	field soil
[Ref.: #2063]	Geographic location (country and/or sea, region)	Vermont
[Ref.: #2063]	Country	USA
[Ref.: #2063]	Country ISO 3 Code	USA
[Ref.: #2063]	Continent	North America

[Ref.: #123846]	Sample type/isolated from	Environment, Soil
[Ref.: #123846]	Geographic location (country and/or sea, region)	Burlington, Vermont
[Ref.: #123846]	Country	United States of America
[Ref.: #123846]	Country ISO 3 Code	USA
[Ref.: #123846]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2063]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123846]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

[Ref.: #2063]

GC-content

66.5 mol%

Availability in culture collections External links

[Ref.: #2063]

Culture collection no.

DSM 87, ATCC 12837, NCIB 8789, CIP 104399, IFO 13581, NCIMB 8789

[Ref.: #81962]

SI-ID 1083

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Metabolism	Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837.	Conde-Avila V, Pena C, Perez-Armendariz B, Loera O, Martinez Valenzuela C, Leyva Morales JB, Jesus Bastidas Bastidas P, Salgado-Lugo H, Ortega Martinez LD	AMB Express	10.1186/s13568-021-01339-w	2021	*
Metabolism	Biological treatment of two-phase olive mill wastewater (TPOMW, alpeorujo): polyhydroxyalkanoates (PHAs) production by Azotobacter strains.	Cerrone F, Sanchez-Peinado Mdel M, Juarez-Jimenez B, Gonzalez-Lopez J, Pozo C	J Microbiol Biotechnol	JMB020-03-23	2010	*
Phylogeny	Production of amino acids by Azotobacter vinelandii and Azotobacter chroococcum with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions.	Revillas JJ, Rodelas B, Pozo C, Martinez-Toledo MV, Lopez JG	Amino Acids	10.1007/s00726-004-0153-x	2005	*
Metabolism	The fatty acid profile of vegetative Azotobacter vinelandii ATCC 12837: growth phase-dependence.	Larsen A, Sliskovic I, Juric D, Pinnock CL, Kullman H, Segstro E, Reinfelds G, Eze MO	Appl Microbiol Biotechnol	10.1007/s00253-005-1908-3	2005	*
Metabolism	Physiology of exopolysaccharide production by Azotobacter vinelandii from 4-hydroxybenzoic acid.	Vargas-Garcia MC, Lopez MJ, Elorrieta MA, Suarez F, Moreno J	J Ind Microbiol Biotechnol	10.1038/sj.jim.7000288	2002	*
Metabolism	Production of B-group vitamins by two Azotobacter strains with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions.	Revillas JJ, Rodelas B, Pozo C, Martinez-Toledo MV, Gonzalez-Lopez J	J Appl Microbiol	10.1046/j.1365-2672.2000.01139.x	2000	*
Metabolism	Carbohydrate fluxes into alginate biosynthesis in Azotobacter vinelandii NCIB 8789: NMR investigations of the triose pools.	Beale JM Jr, Foster JL	Biochemistry	10.1021/bi951922v	1996	*
Phylogeny	Structure elucidation of azotobactin 87, isolated from Azotobacter vinelandii ATCC 12837.	Schaffner EM, Hartmann R, Taraz K, Budzikiewicz H	Z Naturforsch C J Biosci	10.1515/znc-1996-3-402	1996	*
Metabolism	Characterization of the pyoverdines of Azotobacter vinelandii ATCC 12837 with regard to heterogeneity.	Menhart N, Thariath A, Viswanatha T	Biol Met	10.1007/BF01141185	1991	*
Genetics	Growth of Azotobacter vinelandii in dialysed soil medium: studies upon the life cycle.	Ballesteros F, Gonzalez-Lopez J, de la Rubia T, Moreno J, Aneiros J, Ramos-Cormenzana A	Ann Inst Pasteur Microbiol (1985)	10.1016/s0769-2609(86)80005-9	1986	*
Metabolism	Toxicity of dicamba (2-methoxy-3,6-dichlorobenzoic acid) to Azotobacter vinelandii.	Gonzalez-Lopez J, Moreno J, de la Rubia T, Martinez-Toledo MV, Ramos-Cormenzana A	Folia Microbiol (Praha)	10.1007/BF02872928	1984	*
Metabolism	Nitrogen fixation system of tungsten-resistant mutants of Azotobacter vinelandii.	Riddle GD, Simonson JG, Hales BJ, Braymer HD	J Bacteriol	10.1128/jb.152.1.72-80.1982	1982	*
Cultivation	Thermoresistance of azotobacter vinelandii ATCC 12837 in defined and dialysed soil media: filtrable forms.	Gonzalez-Lopez J, Bravo-Mancheno V, Ramos-Cormenzana A	Ann Microbiol (Paris)		1982	*
Enzymology	The purification of glutamine synthetase from Azotobacter and other procaryotes by blue sepharose chromatography.	Lepo JE, Stacey G, Wyss O, Tabita FR	Biochim Biophys Acta	10.1016/0005-2744(79)90311-5	1979	*
Phenotype	Transformation of Azotobacter vinelandii strains unable to fix nitrogen with Rhizobium spp. DNA.	Page WJ	Can J Microbiol	10.1139/m78-038	1978	*
Metabolism	Control of transformation competence in Azotobacter vinelandii by nitrogen catabolite derepression.	Page WJ, Sadoff HL	J Bacteriol	10.1128/jb.125.3.1088-1095.1976	1976	*
Metabolism	Physiological factors affecting transformation of Azotobacter vinelandii.	Page WJ, Sadoff HL	J Bacteriol	10.1128/jb.125.3.1080-1087.1976	1976	*
Metabolism	Relationship between calcium and uroinic acids in the encystment of Azotobacter vinelandii.	Page WJ, Sadoff HL	J Bacteriol	10.1128/jb.122.1.145-151.1975	1975	*
Cultivation	Giant cysts and cysts with multiple central bodies in Azotobacter vinelandii.	Cagle GD, Vela GR	J Bacteriol	10.1128/jb.107.1.315-319.1971	1971	*
Pathogenicity	Chemical composition of Azotobacter vinelandii cysts.	Lin LP, Sadoff HL	J Bacteriol	10.1128/jb.100.1.480-486.1969	1969	*
	Encapsulation of Azotobacter vinelandii ATCC 12837 in Alginate-Na Beads as a Tomato Seedling Inoculant.	Conde-Avila V, Ortega-Martinez LD, Loera O, Perez-Armendariz B, Martinez Valenzuela C	Curr Microbiol	10.1007/s00284-022-02797-6	2022	*
	Transformation of Azotobacter vinelandii with plasmids RP4 (IncP-1 group) and RSF1010 (IncQ group).	David M, Tronchet M, Denarie J	J Bacteriol	10.1128/jb.146.3.1154-1157.1981	1981	*
	Cysts of Azotobacter vinelandii with double coats.	Cagle GD, Vela GR	J Bacteriol	10.1128/jb.112.1.615-617.1972	1972	*
	Freeze-etching of Azotobacter vinelandii: examination of wall, exine, and vesicles.	Cagle GD, Vela GR, Pfister RM	J Bacteriol	10.1128/jb.109.3.1191-1197.1972	1972	*

References

#2063

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 87

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36537 ; Curators of the CIP;

#81962

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1083.1 )

#123846 Collection of Institut Pasteur ; Curators of the CIP; CIP 104399
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Azotobacter vinelandii in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF