Strain identifier
BacDive ID: 12753
Type strain: 
Species: Azotobacter vinelandii
Strain Designation: 3a, Johnstone 3a
Strain history: CIP <- 1995, IFO <- 1973, Y. Einaga, Fac. Sci., Osaka Univ., Japan <- ATCC <- D.B. Johnstone: strain 3a
NCBI tax ID(s): 354 (species)
version 8.1 (current version)
General
@ref: 2063
BacDive-ID: 12753
DSM-Number: 87
keywords: Bacteria, mesophilic, Gram-negative, motile, rod-shaped
description: Azotobacter vinelandii 3a is a mesophilic, Gram-negative, motile bacterium that was isolated from field soil.
NCBI tax id
- NCBI tax id: 354
- Matching level: species
strain history
| @ref | history |
|---|---|
| 2063 | <- ATCC <- D.B. Johnstone, 3a |
| 123846 | CIP <- 1995, IFO <- 1973, Y. Einaga, Fac. Sci., Osaka Univ., Japan <- ATCC <- D.B. Johnstone: strain 3a |
doi: 10.13145/bacdive12753.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Azotobacter
- species: Azotobacter vinelandii
- full scientific name: Azotobacter vinelandii Lipman 1903 (Approved Lists 1980)
@ref: 2063
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Azotobacter
species: Azotobacter vinelandii
full scientific name: Azotobacter vinelandii Lipman 1903
strain designation: 3a, Johnstone 3a
type strain: no
Morphology
cell morphology
- @ref: 123846
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
| @ref | name | growth | composition | link |
|---|---|---|---|---|
| 36537 | MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii | yes | Distilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g) | |
| 2063 | DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) | yes | Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water | https://mediadive.dsmz.de/medium/441 |
| 2063 | AZOTOBACTER MEDIUM (DSMZ Medium 3) | yes | Name: AZOTOBACTER MEDIUM (DSMZ Medium 3) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Glucose 5.0 g/l Mannitol 5.0 g/l K2HPO4 0.9 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.1 g/l FeSO4 x 7 H2O 0.01 g/l Na2MoO4 x 2 H2O 0.005 g/l Distilled water | https://mediadive.dsmz.de/medium/3 |
| 123846 | CIP Medium 1 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 2063 | positive | growth | 30 | mesophilic |
| 36537 | positive | growth | 30 | mesophilic |
| 123846 | positive | growth | 30-41 | |
| 123846 | no | growth | 10 | psychrophilic |
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 123846 | 16947 | citrate | - | carbon source |
| 123846 | 17632 | nitrate | + | reduction |
| 123846 | 16301 | nitrite | - | reduction |
| 123846 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 123846
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 123846
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 123846 | oxidase | + | |
| 123846 | beta-galactosidase | - | 3.2.1.23 |
| 123846 | alcohol dehydrogenase | - | 1.1.1.1 |
| 123846 | gelatinase | - | |
| 123846 | amylase | - | |
| 123846 | DNase | - | |
| 123846 | caseinase | - | 3.4.21.50 |
| 123846 | catalase | + | 1.11.1.6 |
| 123846 | lysine decarboxylase | - | 4.1.1.18 |
| 123846 | ornithine decarboxylase | - | 4.1.1.17 |
| 123846 | urease | + | 3.5.1.5 |
API biotype100
| @ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 123846 | + | + | + | - | - | + | + | + | + | - | - | - | - | - | + | - | - | - | + | - | - | - | + | + | - | + | + | - | + | - | - | + | + | + | - | + | + | - | - | - | + | - | - | - | + | + | - | + | + | + | + | - | - | + | - | - | + | + | - | - | + | - | + | + | + | - | - | + | + | - | - | - | - | - | - | + | + | + | - | + | + | + | + | - | - | - | - | - | + | - | - | - | - | - | - | + | + | - | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 2063 | field soil | Vermont | USA | USA | North America |
| 123846 | Environment, Soil | Burlington, Vermont | United States of America | USA | North America |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Agriculture | #Field |
| #Environmental | #Terrestrial | #Soil |
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 2063 | 1 | Risk group (German classification) |
| 123846 | 1 | Risk group (French classification) |
Sequence information
GC content
- @ref: 2063
- GC-content: 66.5
External links
@ref: 2063
culture collection no.: DSM 87, ATCC 12837, NCIB 8789, CIP 104399, IFO 13581, NCIMB 8789
straininfo link
- @ref: 81962
- straininfo: 1083
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 3492 | Physiological factors affecting transformation of Azotobacter vinelandii. | Page WJ, Sadoff HL | J Bacteriol | 10.1128/jb.125.3.1080-1087.1976 | 1976 | Adenine/metabolism, *Azotobacter/drug effects/growth & development/metabolism, Calcium/pharmacology, Drug Resistance, Microbial, Glucose/pharmacology, Hydrogen-Ion Concentration, Hypoxanthines/metabolism, Magnesium/pharmacology, Mutation, Nitrogenase/metabolism, Phosphates/pharmacology, Rifampin/pharmacology, Temperature, *Transformation, Genetic/drug effects, Uracil/metabolism | Pathogenicity |
| Enzymology | 39606 | The purification of glutamine synthetase from Azotobacter and other procaryotes by blue sepharose chromatography. | Lepo JE, Stacey G, Wyss O, Tabita FR | Biochim Biophys Acta | 10.1016/0005-2744(79)90311-5 | 1979 | Azotobacter/*enzymology, Chromatography, Agarose, Cyanobacteria/enzymology, Electrophoresis, Polyacrylamide Gel, Escherichia coli/enzymology, Glutamate-Ammonia Ligase/*isolation & purification, Rhodobacter sphaeroides/enzymology, Saccharomyces cerevisiae/enzymology | Phylogeny |
| Metabolism | 176141 | Control of transformation competence in Azotobacter vinelandii by nitrogen catabolite derepression. | Page WJ, Sadoff HL | J Bacteriol | 10.1128/jb.125.3.1088-1095.1976 | 1976 | Ammonia/pharmacology, *Azotobacter/enzymology/metabolism, Cyclic AMP/pharmacology, *DNA, Bacterial, Glucose/metabolism, Hydroxybutyrates/metabolism, Molybdenum/pharmacology, Mutation, Nitrogen/*metabolism, Nitrogenase/biosynthesis, *Transformation, Genetic/drug effects, Urea/pharmacology | Enzymology |
| Metabolism | 235508 | Relationship between calcium and uroinic acids in the encystment of Azotobacter vinelandii. | Page WJ, Sadoff HL | J Bacteriol | 10.1128/jb.122.1.145-151.1975 | 1975 | Azotobacter/*cytology/enzymology/metabolism, Calcium/*pharmacology, Carbohydrate Epimerases/metabolism, Cell Wall/metabolism, Hydrogen-Ion Concentration, Hydroxybutyrates/pharmacology, Magnesium/pharmacology, Spectrophotometry, Atomic, Uronic Acids/*biosynthesis | Enzymology |
| Phenotype | 647476 | Transformation of Azotobacter vinelandii strains unable to fix nitrogen with Rhizobium spp. DNA. | Page WJ | Can J Microbiol | 10.1139/m78-038 | 1978 | Azotobacter/enzymology/*genetics/metabolism, DNA, Bacterial/*genetics, Enzyme Repression, *Nitrogen Fixation, Nitrogenase/biosynthesis, Phenotype, Recombination, Genetic, Rhizobium/*genetics, *Transformation, Bacterial | Enzymology |
| Metabolism | 1838001 | Characterization of the pyoverdines of Azotobacter vinelandii ATCC 12837 with regard to heterogeneity. | Menhart N, Thariath A, Viswanatha T | Biol Met | 10.1007/BF01141185 | 1991 | Azotobacter vinelandii/*metabolism, Chromatography, DEAE-Cellulose, Chromatography, High Pressure Liquid, Culture Media, Iron Chelating Agents/chemistry/isolation & purification/metabolism, Kinetics, Magnetic Resonance Spectroscopy, Metals/pharmacology, Nitrogen/metabolism, *Oligopeptides, Pigments, Biological/chemistry/isolation & purification/*metabolism, Siderophores, Spectrometry, Fluorescence, Spectrum Analysis, Temperature | Enzymology |
| Genetics | 2445257 | Growth of Azotobacter vinelandii in dialysed soil medium: studies upon the life cycle. | Ballesteros F, Gonzalez-Lopez J, de la Rubia T, Moreno J, Aneiros J, Ramos-Cormenzana A | Ann Inst Pasteur Microbiol (1985) | 10.1016/s0769-2609(86)80005-9 | 1986 | Azotobacter/analysis/*growth & development/ultrastructure, Cell Cycle, Culture Media, Hydroxybutyrates/analysis, Polymers, RNA, Bacterial/analysis, Soil | Cultivation |
| Cultivation | 4105031 | Giant cysts and cysts with multiple central bodies in Azotobacter vinelandii. | Cagle GD, Vela GR | J Bacteriol | 10.1128/jb.107.1.315-319.1971 | 1971 | Alcohols, Azotobacter/*cytology/growth & development, Cell Division, Cell Wall, Culture Media, Glucose, Microscopy, Electron, Microscopy, Phase-Contrast, *Organoids, Staining and Labeling | |
| 4334764 | Freeze-etching of Azotobacter vinelandii: examination of wall, exine, and vesicles. | Cagle GD, Vela GR, Pfister RM | J Bacteriol | 10.1128/jb.109.3.1191-1197.1972 | 1972 | Azotobacter/*cytology, *Bacteriological Techniques, Cell Membrane, Cell Wall, Flagella, Freeze Etching, *Freezing, Inclusion Bodies, *Microscopy, Electron, Polysaccharides, Bacterial | ||
| Pathogenicity | 4981062 | Chemical composition of Azotobacter vinelandii cysts. | Lin LP, Sadoff HL | J Bacteriol | 10.1128/jb.100.1.480-486.1969 | 1969 | Amino Acids/analysis, Azotobacter/*analysis/cytology/drug effects, Bacterial Proteins/analysis, Calcium/analysis, Carbohydrates/analysis, Centrifugation, Dialysis, Edetic Acid/pharmacology, Fatty Acids/analysis, Hydrogen-Ion Concentration, Lipids/analysis, Magnesium/analysis, Microscopy, Electron, Phosphorus/analysis | |
| 5079077 | Cysts of Azotobacter vinelandii with double coats. | Cagle GD, Vela GR | J Bacteriol | 10.1128/jb.112.1.615-617.1972 | 1972 | Azotobacter/*cytology/growth & development, Cell Membrane, Cell Wall, Cytoplasmic Granules, Hydroxybutyrates, Microscopy, Electron | ||
| Metabolism | 6724435 | Toxicity of dicamba (2-methoxy-3,6-dichlorobenzoic acid) to Azotobacter vinelandii. | Gonzalez-Lopez J, Moreno J, de la Rubia T, Martinez-Toledo MV, Ramos-Cormenzana A | Folia Microbiol (Praha) | 10.1007/BF02872928 | 1984 | Adenosine Triphosphate/metabolism, Azotobacter/*drug effects/metabolism, Benzoates/*toxicity, Dicamba/*toxicity, Soil Pollutants/toxicity | Pathogenicity |
| Metabolism | 6956567 | Nitrogen fixation system of tungsten-resistant mutants of Azotobacter vinelandii. | Riddle GD, Simonson JG, Hales BJ, Braymer HD | J Bacteriol | 10.1128/jb.152.1.72-80.1982 | 1982 | Acetylene/metabolism, Azotobacter/drug effects/genetics/*metabolism, Drug Resistance, Microbial, Molybdenum/pharmacology, Mutation, *Nitrogen Fixation, Nitrogenase/analysis, Oxidation-Reduction, Tungsten/*pharmacology | Pathogenicity |
| 7016829 | Transformation of Azotobacter vinelandii with plasmids RP4 (IncP-1 group) and RSF1010 (IncQ group). | David M, Tronchet M, Denarie J | J Bacteriol | 10.1128/jb.146.3.1154-1157.1981 | 1981 | Azotobacter/*genetics, Escherichia coli/genetics, *Plasmids, *Transformation, Bacterial | ||
| Cultivation | 7149528 | Thermoresistance of azotobacter vinelandii ATCC 12837 in defined and dialysed soil media: filtrable forms. | Gonzalez-Lopez J, Bravo-Mancheno V, Ramos-Cormenzana A | Ann Microbiol (Paris) | 1982 | Azotobacter/cytology/*growth & development, Culture Media, Soil Microbiology, Temperature | ||
| Metabolism | 8605199 | Carbohydrate fluxes into alginate biosynthesis in Azotobacter vinelandii NCIB 8789: NMR investigations of the triose pools. | Beale JM Jr, Foster JL | Biochemistry | 10.1021/bi951922v | 1996 | Alginates/chemistry/*metabolism, Azotobacter vinelandii/*metabolism, *Carbohydrate Metabolism, Carbohydrate Sequence, Carbohydrates/chemistry, Glucose/chemistry/metabolism, Glycolysis, Hexoses/chemistry/metabolism, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure, Trioses/chemistry/*metabolism | Genetics |
| Phylogeny | 8639226 | Structure elucidation of azotobactin 87, isolated from Azotobacter vinelandii ATCC 12837. | Schaffner EM, Hartmann R, Taraz K, Budzikiewicz H | Z Naturforsch C J Biosci | 10.1515/znc-1996-3-402 | 1996 | Amino Acid Sequence, Azotobacter vinelandii/*metabolism, Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry, Magnetic Resonance Spectroscopy, Models, Structural, Molecular Sequence Data, Peptide Biosynthesis, Peptide Fragments/chemistry/isolation & purification, Peptides/*chemistry/isolation & purification, Siderophores, Spectrometry, Mass, Fast Atom Bombardment | Genetics |
| Metabolism | 11021581 | Production of B-group vitamins by two Azotobacter strains with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions. | Revillas JJ, Rodelas B, Pozo C, Martinez-Toledo MV, Gonzalez-Lopez J | J Appl Microbiol | 10.1046/j.1365-2672.2000.01139.x | 2000 | Ammonium Chloride/metabolism, Azotobacter/*metabolism, Benzoates/metabolism, Biotin/biosynthesis, Culture Media, Hydroxybenzoates/metabolism, Niacin/biosynthesis, Pantothenic Acid/biosynthesis, Riboflavin/biosynthesis, Succinates/metabolism, Thiamine/biosynthesis, Time Factors, Vitamin B Complex/*biosynthesis | Cultivation |
| Metabolism | 12242634 | Physiology of exopolysaccharide production by Azotobacter vinelandii from 4-hydroxybenzoic acid. | Vargas-Garcia MC, Lopez MJ, Elorrieta MA, Suarez F, Moreno J | J Ind Microbiol Biotechnol | 10.1038/sj.jim.7000288 | 2002 | Azotobacter vinelandii/growth & development/*metabolism, Carbon/metabolism, Culture Media, Nitrogen/metabolism, Parabens/*metabolism, Polysaccharides, Bacterial/*biosynthesis, Time Factors | Cultivation |
| Metabolism | 15688166 | The fatty acid profile of vegetative Azotobacter vinelandii ATCC 12837: growth phase-dependence. | Larsen A, Sliskovic I, Juric D, Pinnock CL, Kullman H, Segstro E, Reinfelds G, Eze MO | Appl Microbiol Biotechnol | 10.1007/s00253-005-1908-3 | 2005 | Azotobacter vinelandii/growth & development/*metabolism, Fatty Acids/*biosynthesis/isolation & purification, Fatty Acids, Unsaturated/biosynthesis | Enzymology |
| Phylogeny | 15731884 | Production of amino acids by Azotobacter vinelandii and Azotobacter chroococcum with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions. | Revillas JJ, Rodelas B, Pozo C, Martinez-Toledo MV, Lopez JG | Amino Acids | 10.1007/s00726-004-0153-x | 2005 | Amino Acids/*biosynthesis, Azotobacter vinelandii/*growth & development, Hydroxybenzoates/*pharmacology, *Soil Microbiology, Species Specificity, Succinic Acid/*pharmacology | Biotechnology |
| Metabolism | 20372033 | Biological treatment of two-phase olive mill wastewater (TPOMW, alpeorujo): polyhydroxyalkanoates (PHAs) production by Azotobacter strains. | Cerrone F, Sanchez-Peinado Mdel M, Juarez-Jimenez B, Gonzalez-Lopez J, Pozo C | J Microbiol Biotechnol | JMB020-03-23 | 2010 | Anaerobiosis, Azotobacter/chemistry/*metabolism, Biodegradation, Environmental, Bioreactors, Industrial Waste, Magnetic Resonance Spectroscopy, Olive Oil, Plant Oils/*chemistry, Polyhydroxyalkanoates/analysis/*metabolism, Waste Disposal, Fluid/*methods, Water Pollutants, Chemical/*chemistry/metabolism | Biotechnology |
| Metabolism | 34958440 | Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837. | Conde-Avila V, Pena C, Perez-Armendariz B, Loera O, Martinez Valenzuela C, Leyva Morales JB, Jesus Bastidas Bastidas P, Salgado-Lugo H, Ortega Martinez LD | AMB Express | 10.1186/s13568-021-01339-w | 2021 | ||
| 35178619 | Encapsulation of Azotobacter vinelandii ATCC 12837 in Alginate-Na Beads as a Tomato Seedling Inoculant. | Conde-Avila V, Ortega-Martinez LD, Loera O, Perez-Armendariz B, Martinez Valenzuela C | Curr Microbiol | 10.1007/s00284-022-02797-6 | 2022 | Alginates, *Azotobacter vinelandii/genetics, *Lycopersicon esculentum, Rhizosphere, Seedlings |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 2063 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 87) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-87 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 36537 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16303 | ||
| 81962 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID1083.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
| 123846 | Curators of the CIP | Collection of Institut Pasteur (CIP 104399) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104399 |