Azotobacter vinelandii (DSM 366, ATCC 13705, OP)

Name: Azotobacter vinelandii (DSM 366, ATCC 13705, OP)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12741

Type strain

Strain Designation OP

Culture col. no. DSM 366 ATCC 13705 OP

NCBI tax ID(s) 354

Special Collections DSMZ

History <- J.R. Postgate, P.W. Wilson OP

Links

Azotobacter vinelandii OP is a bacterium of the family Pseudomonadaceae.

Bacteria

Name and taxonomic classification

SI-ID 1084

LMG 3878,ATCC 13705,DSM 366,NCIB 11614,NCIMB 11614,BCRC 15759,CCRC 15759

@ref 20215

Last LPSN update 2026-02-09 11:20:55

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Azotobacter

Species Azotobacter vinelandii

Full scientific name Azotobacter vinelandii Lipman 1903 (Approved Lists 1980)

BacDive ID	Other strains from **Azotobacter vinelandii** (16)	Type strain
12749	A. vinelandii 16, DSM 2289, ATCC 478, VKM B-1617, JCM 21475, ... (type strain)
12739	A. vinelandii 567, DSM 279, CCUG 51007
12740	A. vinelandii 73, DSM 332
12742	A. vinelandii Kg 2, DSM 382
12743	A. vinelandii 1610, DSM 389, IMG 1610
12744	A. vinelandii W2, DSM 390
12745	A. vinelandii 5R, DSM 395
12746	A. vinelandii DSM 399, RH Rh6, RH 6
12747	A. vinelandii DSM 576, NCIB 9068
12748	A. vinelandii 135, DSM 720, ATCC 17962, BKM B-547, VKM ...
12750	A. vinelandii VI, DSM 2290, ATCC 7496
12751	A. vinelandii 1484, DSM 85, IMG 1484
12752	A. vinelandii 1484, Claus 1484T, DSM 86
12753	A. vinelandii 3a, Johnstone 3a, DSM 87, ATCC 12837, NCIB ...
12754	A. vinelandii 93-541b, DSM 13529
162709	A. vinelandii JCM 20040, ATCC 9046, CECT 9203, IAM 1078

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
156	DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441)		Medium recipe at MediaDive	Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water
156	AZOTOBACTER MEDIUM (DSMZ Medium 3)		Medium recipe at MediaDive	Name: AZOTOBACTER MEDIUM (DSMZ Medium 3) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Glucose 5.0 g/l Mannitol 5.0 g/l K2HPO4 0.9 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.1 g/l FeSO4 x 7 H2O 0.01 g/l Na2MoO4 x 2 H2O 0.005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
156	positive	growth	30

Physiology and metabolism

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
156	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Tailoring 3HV Fraction in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Azotobacter vinelandii Through Oxygen and Carbon Limitation in Continuous Cultures.	Perez A, Garcia A, Urtuvia V, Pena C, Diaz-Barrera A.	Polymers (Basel)	10.3390/polym17192578	2025
	Extended batch cultures for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production by Azotobacter vinelandii OP growing at different aeration rates.	Urtuvia V, Ponce B, Andler R, Pena C, Diaz-Barrera A.	3 Biotech	10.1007/s13205-022-03380-3	2022
	Selenocyanate derived Se-incorporation into the nitrogenase Fe protein cluster.	Buscagan TM, Kaiser JT, Rees DC.	Elife	10.7554/elife.79311	2022
	Structural evolution of nitrogenase states under alkaline turnover.	Warmack RA, Rees DC.	Nat Commun	10.1038/s41467-024-54713-0	2024
	Structural consequences of turnover-induced homocitrate loss in nitrogenase.	Warmack RA, Maggiolo AO, Orta A, Wenke BB, Howard JB, Rees DC.	Nat Commun	10.1038/s41467-023-36636-4	2023
	Complete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6.	Noar JD, Bruno-Barcena JM.	Genome Announc	10.1128/genomea.00313-13	2013
Metabolism	The Siderophore Metabolome of Azotobacter vinelandii.	Baars O, Zhang X, Morel FM, Seyedsayamdost MR.	Appl Environ Microbiol	10.1128/aem.03160-15	2016
Metabolism	RpoS expression and the general stress response in Azotobacter vinelandii during carbon and nitrogen diauxic shifts.	Sandercock JR, Page WJ.	J Bacteriol	10.1128/jb.01571-06	2008
Enzymology	Identification of two catalases in Azotobacter vinelandii: a KatG homologue and a novel bacterial cytochrome c catalase, CCCAv.	Sandercock JR, Page WJ.	J Bacteriol	10.1128/jb.01572-06	2008
	The DeLey-Doudoroff Pathway of Galactose Metabolism in Azotobacter vinelandii.	Wong TY, Yao XT.	Appl Environ Microbiol	10.1128/aem.60.6.2065-2068.1994	1994
Metabolism	Nitrogenase activity and regeneration of the cellular ATP pool in Azotobacter vinelandii adapted to different oxygen concentrations.	Linkerhagner K, Oelze J.	J Bacteriol	10.1128/jb.179.4.1362-1367.1997	1997
	Diauxic Growth of Azotobacter vinelandii on Galactose and Glucose: Regulation of Glucose Transport by Another Hexose.	Wong TY, Pei H, Bancroft K, Childers GW.	Appl Environ Microbiol	10.1128/aem.61.2.430-433.1995	1995
Metabolism	Role of GlnK in NifL-mediated regulation of NifA activity in Azotobacter vinelandii.	Rudnick P, Kunz C, Gunatilaka MK, Hines ER, Kennedy C.	J Bacteriol	10.1128/jb.184.3.812-820.2002	2002
Metabolism	Dependence of oxygen-tolerant nitrogenase activity on divalent cations in Azotobacter vinelandii.	Peterson JB.	J Bacteriol	10.1128/jb.174.10.3399-3402.1992	1992
Metabolism	Role of molybdate and other transition metals in the accumulation of protochelin by Azotobacter vinelandii.	Cornish AS, Page WJ.	Appl Environ Microbiol	10.1128/aem.66.4.1580-1586.2000	2000
Metabolism	Cellular ATP levels and nitrogenase switchoff upon oxygen stress in chemostat cultures of Azotobacter vinelandii.	Linkerhagner K, Oelze J.	J Bacteriol	10.1128/jb.177.18.5289-5293.1995	1995
	Alternative Function of the Electron Transport System in Azotobacter vinelandii: Removal of Excess Reductant by the Cytochrome d Pathway.	Liu J, Lee F, Lin C, Yao X, Davenport JW, Wong T.	Appl Environ Microbiol	10.1128/aem.61.11.3998-4003.1995	1995
	Stimulation of Agrobacterium tumefaciens Growth by Azotobacter vinelandii Ferrisiderophores.	Page WJ, Dale PL.	Appl Environ Microbiol	10.1128/aem.51.2.451-454.1986	1986
Metabolism	Control of diauxic growth of Azotobacter vinelandii on acetate and glucose.	Tauchert K, Jahn A, Oelze J.	J Bacteriol	10.1128/jb.172.11.6447-6451.1990	1990
Enzymology	Dependence of nitrogenase switch-off upon oxygen stress on the nitrogenase activity in Azotobacter vinelandii.	Kuhla J, Oelze J.	J Bacteriol	10.1128/jb.170.11.5325-5329.1988	1988
Genetics	Azotobacter Genomes: The Genome of Azotobacter chroococcum NCIMB 8003 (ATCC 4412).	Robson RL, Jones R, Robson RM, Schwartz A, Richardson TH.	PLoS One	10.1371/journal.pone.0127997	2015
	Hyperproduction of Poly-beta-Hydroxybutyrate during Exponential Growth of Azotobacter vinelandii UWD.	Page WJ, Knosp O.	Appl Environ Microbiol	10.1128/aem.55.6.1334-1339.1989	1989
Enzymology	Levels and activities of nitrogenase proteins in Azotobacter vinelandii grown at different dissolved oxygen concentrations.	Dingler C, Kuhla J, Wassink H, Oelze J.	J Bacteriol	10.1128/jb.170.5.2148-2152.1988	1988
Metabolism	The biphenyl- and 4-chlorobiphenyl-catabolic transposon Tn4371, a member of a new family of genomic islands related to IncP and Ti plasmids.	Toussaint A, Merlin C, Monchy S, Benotmane MA, Leplae R, Mergeay M, Springael D.	Appl Environ Microbiol	10.1128/aem.69.8.4837-4845.2003	2003
	Evidence for two nonidentical subunits of bacterioferritin from Azotobacter vinelandii.	Harker AR, Wullstein LH.	J Bacteriol	10.1128/jb.162.2.651-655.1985	1985
	Siderophores Produced by Nitrogen-Fixing Azotobacter vinelandii OP in Iron-Limited Continuous Culture.	Fekete FA, Spence JT, Emery T.	Appl Environ Microbiol	10.1128/aem.46.6.1297-1300.1983	1983
Metabolism	Proton-coupled calcium transport by intact cells of Azotobacter vinelandii.	Barnes EM.	J Bacteriol	10.1128/jb.143.2.1086-1089.1980	1980
Metabolism	Role of glutamine synthetase in the uptake and metabolism of methylammonium by Azotobacter vinelandii.	Barnes EM, Zimniak P, Jayakumar A.	J Bacteriol	10.1128/jb.156.2.752-757.1983	1983
Metabolism	Transport of ammonium and methylammonium ions by Azotobacter vinelandii.	Barnes EM, Zimniak P.	J Bacteriol	10.1128/jb.146.2.512-516.1981	1981
Metabolism	Nitrogen fixation system of tungsten-resistant mutants of Azotobacter vinelandii.	Riddle GD, Simonson JG, Hales BJ, Braymer HD.	J Bacteriol	10.1128/jb.152.1.72-80.1982	1982
Genetics	Broad-host-range properties of plasmid RK2: importance of overlapping genes encoding the plasmid replication initiation protein TrfA.	Fang FC, Helinski DR.	J Bacteriol	10.1128/jb.173.18.5861-5868.1991	1991

References

#156	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 366
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Azotobacter vinelandii (DSM 366, ATCC 13705, OP)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

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