Name: Shouchella oshimensis DSM 19099
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1273

Type strain:

Species: Shouchella oshimensis

Culture col. no.: DSM 19099, JCM 13820, MLB 2, MTCC 7633, CIP 109550, LMG 24751

Strain history: CIP <- 2007, JCM <- 2006, R.K. Jain, MTCC, Chandigarh, India: strain MLB2

NCBI tax ID(s): 300825 (species)

Other strains from Shouchella oshimensis

S. oshimensis K11, DSM 18940, CIP 108751, JCM ... (type strain)
S. oshimensis P203, DSM 19153, NCIMB 14288

Section

Shouchella oshimensis DSM 19099 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from soil.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Shouchella*
	Species	**Shouchella oshimensis**
	Full Scientific Name (LPSN)	Shouchella oshimensis (Yumoto et al. 2005) Joshi et al. 2022

	Synonym	Shouchella plakortidis
	Synonym	Shouchella lehensis
	Synonym	Alkalihalobacillus lehensis
	Synonym	Alkalihalobacillus oshimensis
	Synonym	Alkalicoccobacillus plakortidis
	Synonym	Bacillus lehensis
	Synonym	Bacillus plakortidis
	Synonym	Alkalihalobacillus plakortidis
	Synonym	Bacillus oshimensis

Information on morphological and physiological properties Morphology

[Ref.: #31900]	Gram stain	positive
[Ref.: #120575]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31900]	Cell length	2.4 µm

[Ref.: #31900]	Cell width	0.65 µm

[Ref.: #31900]	Cell shape	rod-shaped
[Ref.: #120575]	Cell shape	rod-shaped

[Ref.: #31900]	Motility	yes
[Ref.: #120575]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %93.887

[Ref.: #31900]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #37648]

Culture medium

Marine agar (MA)

[Ref.: #37648]

Culture medium growth

[Ref.: #37648]

Culture medium composition

expand / minimize

[Ref.: #120575]

Culture medium

CIP Medium 13

[Ref.: #120575]

Culture medium growth

[Ref.: #120575]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #120575]

Culture medium

CIP Medium 328

[Ref.: #120575]

Culture medium growth

[Ref.: #120575]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #31900]	growth	10-37 °C
[Ref.: #31900]	optimum	25 °C
[Ref.: #37648]	growth	25 °C
[Ref.: #67770]	growth	25 °C

[Ref.: #31900]	Temperature range	mesophilic
[Ref.: #37648]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31900]			optimum	8
[Ref.: #31900]			growth	07-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31900]

Oxygen tolerance

aerobe

[Ref.: #31900]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31900]		NaCl		growth	0-12	%
[Ref.: #31900]		NaCl		optimum	6	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31900]	2-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #31900]	2-oxopentanoate ChEBI	+	carbon source
[Ref.: #31900]	acetate ChEBI	+	carbon source
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	dextrin ChEBI	+	carbon source
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	gelatin ChEBI	+	carbon source
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	glycerol ChEBI	+	carbon source
[Ref.: #31900]	glycogen ChEBI	+	carbon source
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	L-arabitol ChEBI	+	carbon source
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	lactose ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	maltose ChEBI	+	carbon source
[Ref.: #31900]	mannitol ChEBI	+	carbon source
[Ref.: #31900]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	nitrate ChEBI	+	reduction
[Ref.: #120575]	nitrate ChEBI	-	reduction
[Ref.: #120575]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	propionate ChEBI	+	carbon source
[Ref.: #31900]	pyruvate ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	sorbitol ChEBI	+	carbon source
[Ref.: #31900]	sucrose ChEBI	+	carbon source
[Ref.: #31900]	trehalose ChEBI	+	carbon source
[Ref.: #31900]	tween 40 ChEBI	+	carbon source
[Ref.: #31900]	tween 60 ChEBI	+	carbon source
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31900]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120575]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31900]	catalase	+	1.11.1.6
[Ref.: #120575]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31900]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #31900]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #120575]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120575]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120575]	11591	not determinedn.d.	+/-	-	-	+/-	+/-	+/-	-	-	-	-	+/-	+/-	+/-	-	-	-	-	+/-	-	-	-	+/-	-	-	+/-	-	+/-	+/-	-	-	+/-	+/-	-	+/-	+/-	+/-	+/-	-	-	+/-	-	-	-	-	+/-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120575]	11947	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7890]	Sample type/isolated from	soil
[Ref.: #7890]	Geographic location (country and/or sea, region)	Leh
[Ref.: #7890]	Country	India
[Ref.: #7890]	Country ISO 3 Code	IND
[Ref.: #7890]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil collected from Leh
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia

[Ref.: #120575]	Sample type/isolated from	Environment, Soil
[Ref.: #120575]	Geographic location (country and/or sea, region)	Leh
[Ref.: #120575]	Country	India
[Ref.: #120575]	Country ISO 3 Code	IND
[Ref.: #120575]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY793550 (>99% sequence identity) for Alkalihalobacillus from Microbeatlas

Aquatic Samples	620
Soil Samples	2128
Animal Samples	2142
Plant Samples	604
Total Samples	5494

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7890]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120575]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7890]	Bacillus lehensis strain MLB2 16S ribosomal RNA gene, partial sequence	AY793550	1525	ENA	300825 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Shouchella lehensis DSM 19099	GCA_004375095	scaffold	GenBank	300825 tax ID
[Ref.: #66792]	Bacillus lehensis strain DSM 19099	300825.3	wgs	PATRIC	300825 tax ID	*

[Ref.: #7890]	GC-content	41.4 mol%
[Ref.: #67770]	GC-content	41.4 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkalihalobacillus lehensis DSM 19099 NCBI: GCA_004375095.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus lehensis strain DSM 19099 PATRIC: 300825.3
[Ref.: #69480]	gram-positive	yes	91.104	yes
[Ref.: #69480]	anaerobic	no	99.232	no
[Ref.: #69480]	halophile	yes	93.881	yes
[Ref.: #69480]	spore-forming	yes	94.481	no
[Ref.: #69480]	glucose-util	yes	91.187	no
[Ref.: #69480]	motile	yes	87.643	yes
[Ref.: #69480]	flagellated	yes	58.421	no
[Ref.: #69480]	aerobic	yes	92.517	yes
[Ref.: #69480]	glucose-ferment	no	90.634	no
[Ref.: #69480]	thermophile	no	99.334	yes

Availability in culture collections External links

[Ref.: #7890]

Culture collection no.

DSM 19099, JCM 13820, MLB 2, MTCC 7633, CIP 109550, LMG 24751

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus miscanthi sp. nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.	Shin B, Park C, Lee BH, Lee KE, Park W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003982	2020	*
Phylogeny	Genome-based reclassification of Bacillus plakortidis Borchert et al. 2007 and Bacillus lehensis Ghosh et al. 2007 as a later heterotypic synonym of Bacillus oshimensis Yumoto et al. 2005; Bacillus rhizosphaerae Madhaiyan et al. 2011 as a later heterotypic synonym of Bacillus clausii Nielsen et al. 1995.	Liu GH, Narsing Rao MP, Dong ZY, Wang JP, Che JM, Chen QQ, Sengonca C, Liu B, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-019-01299-z	2019	*
Phylogeny	Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil.	Ghosh A, Bhardwaj M, Satyanarayana T, Khurana M, Mayilraj S, Jain RK	Int J Syst Evol Microbiol	10.1099/ijs.0.64617-0	2007	*

References

#7890

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19099

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31900

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28158 (see below)

#37648 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#120575 Collection of Institut Pasteur ; Curators of the CIP; CIP 109550

#28158

IJSEM 238 2007 ( DOI 10.1099/ijs.0.64617-0 , PubMed 17267957 )

* These data were automatically processed and therefore are not curated

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Alkalihalobacillus lehensis DSM 19099

Bacillus lehensis strain DSM 19099

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