Name: Shouchella oshimensis K11
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1259

Type strain:

Species: Shouchella oshimensis

Strain Designation: K11

Culture col. no.: DSM 18940, CIP 108751, JCM 12663, NCIMB 14023

Strain history: CIP <- 2005, JCM <- I. Yumoto, Sapporo, Japan: strain K11

NCBI tax ID(s): 290588 (species)

SI-ID 137998

JCM 12663, NCIMB 14023, CIP 108751, DSM 18940, LMG 24731

Other strains from Shouchella oshimensis

S. oshimensis DSM 19099, JCM 13820, MLB 2, MTCC ... (type strain)
S. oshimensis P203, DSM 19153, NCIMB 14288

Section

Shouchella oshimensis K11 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Shouchella*
	Species	**Shouchella oshimensis**
	Full Scientific Name (LPSN)	Shouchella oshimensis (Yumoto et al. 2005) Joshi et al. 2022

	Synonym	Shouchella plakortidis
	Synonym	Shouchella lehensis
	Synonym	Alkalihalobacillus lehensis
	Synonym	Alkalihalobacillus oshimensis
	Synonym	Alkalicoccobacillus plakortidis
	Synonym	Bacillus lehensis
	Synonym	Bacillus plakortidis
	Synonym	Alkalihalobacillus plakortidis
	Synonym	Bacillus oshimensis

Information on morphological and physiological properties Morphology

[Ref.: #31410]	Gram stain	positive
[Ref.: #121457]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31410]	Cell length	2.75 µm

[Ref.: #31410]	Cell width	0.8 µm

[Ref.: #31410]	Cell shape	rod-shaped
[Ref.: #121457]	Cell shape	rod-shaped

[Ref.: #31410]	Motility	no
[Ref.: #121457]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %93.283

[Ref.: #121457]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7798]

Culture medium

HORIKOSHI ALKALINE (DSMZ Medium 940)

[Ref.: #7798]

Culture medium growth

[Ref.: #7798]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7798]

Culture medium composition

expand / minimize

[Ref.: #37639]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37639]

Culture medium growth

[Ref.: #37639]

Culture medium composition

expand / minimize

[Ref.: #121457]

Culture medium

CIP Medium 72

[Ref.: #121457]

Culture medium growth

[Ref.: #121457]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121457]

Culture medium

CIP Medium 648

[Ref.: #121457]

Culture medium growth

[Ref.: #121457]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7798]		growth	30 °C
[Ref.: #31410]		growth	13-41 °C
[Ref.: #31410]		optimum	30 °C
[Ref.: #37639]		growth	30 °C
[Ref.: #67770]		growth	27 °C
[Ref.: #121457]		growth	30-37 °C
[Ref.: #121457]	no	growth	55 °C

[Ref.: #7798]	Temperature range	mesophilic
[Ref.: #31410]	Temperature range	mesophilic
[Ref.: #37639]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121457]	Temperature range	mesophilic
[Ref.: #121457]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #31410]			optimum	8.5
[Ref.: #31410]			growth	07-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31410]	Oxygen tolerance	aerobe
[Ref.: #121457]	Oxygen tolerance	obligate aerobe

[Ref.: #31410]	Ability of spore formation	yes
[Ref.: #121457]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31410]		NaCl		growth	0-20	%
[Ref.: #31410]		NaCl		optimum	7	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31410]	gelatin ChEBI	+	carbon source
[Ref.: #31410]	glycerol ChEBI	+	carbon source
[Ref.: #31410]	maltose ChEBI	+	carbon source
[Ref.: #31410]	mannitol ChEBI	+	carbon source
[Ref.: #31410]	mannose ChEBI	+	carbon source
[Ref.: #31410]	melibiose ChEBI	+	carbon source
[Ref.: #121457]	nitrate ChEBI	-	reduction
[Ref.: #121457]	nitrate ChEBI	-	respiration
[Ref.: #121457]	nitrite ChEBI	-	reduction
[Ref.: #31410]	raffinose ChEBI	+	carbon source
[Ref.: #31410]	ribose ChEBI	+	carbon source
[Ref.: #31410]	trehalose ChEBI	+	carbon source
[Ref.: #31410]	tween 20 ChEBI	+	carbon source
[Ref.: #31410]	tween 40 ChEBI	+	carbon source
[Ref.: #31410]	tween 60 ChEBI	+	carbon source
[Ref.: #31410]	tween 80 ChEBI	+	carbon source
[Ref.: #31410]	xylitol ChEBI	+	carbon source
[Ref.: #31410]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121457]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121457]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31410]	catalase	+	1.11.1.6
[Ref.: #121457]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31410]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121457]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121457]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121457]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121457]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121457]	11433	-	-	+	+	-	+	-	-	-	+	+	+	+	-	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7798]	Sample type/isolated from	soil
[Ref.: #7798]	Geographic location (country and/or sea, region)	Hokkaido
[Ref.: #7798]	Country	Japan
[Ref.: #7798]	Country ISO 3 Code	JPN
[Ref.: #7798]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Hokkaido
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #121457]	Sample type/isolated from	Environment, Soil
[Ref.: #121457]	Geographic location (country and/or sea, region)	Hokkaido
[Ref.: #121457]	Country	Japan
[Ref.: #121457]	Country ISO 3 Code	JPN
[Ref.: #121457]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB188090 (>99% sequence identity) for Alkalihalobacillus from Microbeatlas

Aquatic Samples	620
Soil Samples	2128
Animal Samples	2142
Plant Samples	604
Total Samples	5494

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7798]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121457]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7798]	Bacillus oshimensis gene for 16S rRNA, partial sequence, strain:K11	AB188090	1467	ENA	290588 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Shouchella oshimensis DSM 18940	GCA_002019765	scaffold	GenBank	290588 tax ID
[Ref.: #66792]	Bacillus oshimensis strain DSM 18940	290588.4	wgs	PATRIC	290588 tax ID	*

[Ref.: #7798]	GC-content	40.8 mol%
[Ref.: #67770]	GC-content	40.8 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkalihalobacillus oshimensis DSM 18940 NCBI: GCA_002019765.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus oshimensis strain DSM 18940 PATRIC: 290588.4
[Ref.: #69480]	gram-positive	yes	88.765	yes
[Ref.: #69480]	anaerobic	no	99.351	yes
[Ref.: #69480]	halophile	yes	93.151	yes
[Ref.: #69480]	spore-forming	yes	94.562	no
[Ref.: #69480]	glucose-util	yes	90.332	no
[Ref.: #69480]	motile	yes	88.006	no
[Ref.: #69480]	flagellated	yes	65.435	yes
[Ref.: #69480]	aerobic	yes	91.808	yes
[Ref.: #69480]	thermophile	no	99.204	yes
[Ref.: #69480]	glucose-ferment	no	88.88	no

Availability in culture collections External links

[Ref.: #7798]

Culture collection no.

DSM 18940, CIP 108751, JCM 12663, NCIMB 14023

[Ref.: #70915]

SI-ID 137998

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genome-based reclassification of Bacillus plakortidis Borchert et al. 2007 and Bacillus lehensis Ghosh et al. 2007 as a later heterotypic synonym of Bacillus oshimensis Yumoto et al. 2005; Bacillus rhizosphaerae Madhaiyan et al. 2011 as a later heterotypic synonym of Bacillus clausii Nielsen et al. 1995.	Liu GH, Narsing Rao MP, Dong ZY, Wang JP, Che JM, Chen QQ, Sengonca C, Liu B, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-019-01299-z	2019	*
Phylogeny	Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil.	Ghosh A, Bhardwaj M, Satyanarayana T, Khurana M, Mayilraj S, Jain RK	Int J Syst Evol Microbiol	10.1099/ijs.0.64617-0	2007	*
Phylogeny	Bacillus oshimensis sp. nov., a moderately halophilic, non-motile alkaliphile.	Yumoto I, Hirota K, Goto T, Nodasaka Y, Nakajima K	Int J Syst Evol Microbiol	10.1099/ijs.0.63488-0	2005	*

References

#7798

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18940

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31410

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27719 (see below)

#37639 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70915

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID137998.1 )

#121457 Collection of Institut Pasteur ; Curators of the CIP; CIP 108751

#27719

IJSEM 907 2005 ( DOI 10.1099/ijs.0.63488-0 , PubMed 15774684 )

* These data were automatically processed and therefore are not curated

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Alkalihalobacillus oshimensis DSM 18940

Bacillus oshimensis strain DSM 18940

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