Name: Acidipropionibacterium microaerophilum M5
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12656

Type strain:

Species: Acidipropionibacterium microaerophilum

Strain Designation: M5

Culture col. no.: DSM 13435, CIP 109962, CNCM I-2360

Strain history: CIP <- 2009, DSMZ <- M. Koussémon, Provence and Mediterranean Univ., Marseille, France: strain M5

NCBI tax ID(s): 118367 (species)

SI-ID 49470

DSM 13435, CIP 109962

Special Collections

DSMZ
CIP

Section

Acidipropionibacterium microaerophilum M5 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from olive mill wastewater.

Gram-positive
rod-shaped
anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Propionibacteriaceae*
	Genus	*Acidipropionibacterium*
	Species	**Acidipropionibacterium microaerophilum**
	Full Scientific Name (LPSN)	Acidipropionibacterium microaerophilum (Koussémon et al. 2001) Scholz and Kilian 2016

Synonym

Propionibacterium microaerophilum

Information on morphological and physiological properties Morphology

[Ref.: #120946]	Gram stain	positive

[Ref.: #120946]	Cell shape	rod-shaped

[Ref.: #120946]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5046]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #5046]

Culture medium growth

[Ref.: #5046]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5046]

Culture medium composition

expand / minimize

[Ref.: #42207]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #42207]

Culture medium growth

[Ref.: #42207]

Culture medium composition

expand / minimize

[Ref.: #120946]

Culture medium

CIP Medium 20

[Ref.: #120946]

Culture medium growth

[Ref.: #120946]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #5046]			growth	30 °C
[Ref.: #42207]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5046]	Oxygen tolerance	anaerobe
[Ref.: #120946]	Oxygen tolerance	facultative anaerobe

[Ref.: #5046]	Murein short key	A41.01
[Ref.: #5046]	Murein types	A3gamma LL-Dpm-Gly

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	+	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #120946]	nitrate ChEBI	+	reduction
[Ref.: #68380]	nitrate ChEBI	+	reduction	* from API rID32A
[Ref.: #120946]	nitrite ChEBI	-	reduction
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #120946]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A, API zym
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A, API zym
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A, API zym
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A, API zym
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A, API zym
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A, API zym
[Ref.: #120946]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120946]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	+	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #120946]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #5046]	12597	-	-	-	+	-	+	+	-	-	-	+	+	-	-	+	-	+	+	+	-	-	+/-	-	-	-	+/-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120946]	12258	-	+	+	-	-	+	-	+	-	-	+	+	-	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5046]	Sample type/isolated from	olive mill wastewater
[Ref.: #5046]	Country	France
[Ref.: #5046]	Country ISO 3 Code	FRA
[Ref.: #5046]	Continent	Europe

[Ref.: #120946]	Sample type/isolated from	Environment, Olive mill wastwater
[Ref.: #120946]	Country	France
[Ref.: #120946]	Country ISO 3 Code	FRA
[Ref.: #120946]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Industrial wastewater

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5046]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120946]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5046]	Propionibacterium microaerophilus 16S ribosomal RNA gene, partial sequence	AF234623	1524	GenBank ENA	118367 tax ID
[Ref.: #20218]	Propionibacterium microaerophilum strain DSMZ 13435 16S ribosomal RNA gene, partial sequence	AY883043	461	GenBank ENA	118367 tax ID	*

[Ref.: #5046]

GC-content

68 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #5046]

Culture collection no.

DSM 13435, CIP 109962, CNCM I-2360

[Ref.: #81869]

SI-ID 49470

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Propionibacterium microaerophilum sp. nov., a microaerophilic bacterium isolated from olive mill wastewater.	Koussemon M, Combet-Blanc Y, Patel BK, Cayol JL, Thomas P, Garcia JL, Ollivier B	Int J Syst Evol Microbiol	10.1099/00207713-51-4-1373	2001	*

References

#5046

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13435

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#42207 ; Curators of the CIP;

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#81869

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49470.1 )

#120946 Collection of Institut Pasteur ; Curators of the CIP; CIP 109962
* These data were automatically processed and therefore are not curated

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