Strain identifier

BacDive ID: 12656

Type strain: Yes

Species: Acidipropionibacterium microaerophilum

Strain Designation: M5

Strain history: CIP <- 2009, DSMZ <- M. Koussémon, Provence and Mediterranean Univ., Marseille, France: strain M5

NCBI tax ID(s): 118367 (species)

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General

@ref: 5046

BacDive-ID: 12656

DSM-Number: 13435

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Acidipropionibacterium microaerophilum M5 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from olive mill wastewater.

NCBI tax id

  • NCBI tax id: 118367
  • Matching level: species

strain history

@refhistory
5046<- M. Koussémon; M5
120946CIP <- 2009, DSMZ <- M. Koussémon, Provence and Mediterranean Univ., Marseille, France: strain M5

doi: 10.13145/bacdive12656.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Acidipropionibacterium
  • species: Acidipropionibacterium microaerophilum
  • full scientific name: Acidipropionibacterium microaerophilum (Koussémon et al. 2001) Scholz and Kilian 2016

  • synonyms

    • @ref: 20215
    • synonym: Propionibacterium microaerophilum

@ref: 5046

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Acidipropionibacterium

species: Acidipropionibacterium microaerophilum

full scientific name: Acidipropionibacterium microaerophilum (Koussémon et al. 2001) Scholz and Kilian 2016

strain designation: M5

type strain: yes

Morphology

cell morphology

  • @ref: 120946
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5046PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
42207MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
120946CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
5046positivegrowth30mesophilic
42207positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5046anaerobe
120946facultative anaerobe

murein

  • @ref: 5046
  • murein short key: A41.01
  • type: A3gamma LL-Dpm-Gly

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12094617632nitrate+reduction
12094616301nitrite-reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate+reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
12094635581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
120946oxidase-
120946catalase-1.11.1.6
120946urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120946-++--+-+--++-+-+++--

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
5046---+-++---++--+-+++--+/----+/----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5046olive mill wastewaterFranceFRAEurope
120946Environment, Olive mill wastwaterFranceFRAEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Industrial wastewater

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
50461Risk group (German classification)
1209461Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Propionibacterium microaerophilum strain DSMZ 13435 16S ribosomal RNA gene, partial sequenceAY883043461ena118367
5046Propionibacterium microaerophilus 16S ribosomal RNA gene, partial sequenceAF2346231524ena118367

GC content

  • @ref: 5046
  • GC-content: 68
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 5046

culture collection no.: DSM 13435, CIP 109962, CNCM I-2360

straininfo link

  • @ref: 81869
  • straininfo: 49470

literature

  • topic: Phylogeny
  • Pubmed-ID: 11491335
  • title: Propionibacterium microaerophilum sp. nov., a microaerophilic bacterium isolated from olive mill wastewater.
  • authors: Koussemon M, Combet-Blanc Y, Patel BK, Cayol JL, Thomas P, Garcia JL, Ollivier B
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-4-1373
  • year: 2001
  • mesh: Aerobiosis, Anaerobiosis, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Food Industry, Glucose/metabolism, Industrial Waste, Microscopy, Electron, Molecular Sequence Data, Olive Oil, Phylogeny, Plant Oils, Propionibacterium/*classification/genetics/*isolation & purification/metabolism, Water Microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5046Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13435)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13435
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42207Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7764
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
81869Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49470.1StrainInfo: A central database for resolving microbial strain identifiers
120946Curators of the CIPCollection of Institut Pasteur (CIP 109962)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109962