Name: Prevotella amnii DSM 23384
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12562

Type strain:

Species: Prevotella amnii

Culture col. no.: DSM 23384, CCUG 53648, JCM 14753, KCTC 15072

Strain history: CCUG 53648 <-- S. Kawash.

NCBI tax ID(s): 1122979 (strain), 419005 (species)

SI-ID 303425

CCUG 53648, JCM 14753, CIP 109456, DSM 23384

Special Collections

Other strains from Prevotella amnii

Section

Prevotella amnii DSM 23384 is an anaerobe, Gram-negative, rod-shaped human pathogen that was isolated from amniotic fluid of a 29-year-old female.

Gram-negative
rod-shaped
anaerobe
human pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Prevotella*
	Species	**Prevotella amnii**
	Full Scientific Name (LPSN)	Prevotella amnii Lawson et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #32204]	Gram stain	negative
[Ref.: #68367]	Gram stain	negative * from API 20A
[Ref.: #69480]	Gram stain	negative Confidence in %95.734

[Ref.: #32204]	Cell length	1.9 µm

[Ref.: #32204]	Cell width	1 µm

[Ref.: #32204]	Cell shape	rod-shaped
[Ref.: #68367]	Cell shape	rod-shaped * from API 20A

[Ref.: #32204]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90.5

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16819]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16819]

Culture medium growth

[Ref.: #16819]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16819]

Culture medium composition

expand / minimize

[Ref.: #16819]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #16819]

Culture medium growth

[Ref.: #16819]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16819]

Culture medium composition

expand / minimize

[Ref.: #16819]

Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

[Ref.: #16819]

Culture medium growth

[Ref.: #16819]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16819]

Culture medium composition

expand / minimize

[Ref.: #16819]

Culture medium

YCFA-MEDIUM (MODIFIED) (DSMZ Medium 1611)

[Ref.: #16819]

Culture medium growth

[Ref.: #16819]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16819]	growth	37 °C
[Ref.: #32204]	growth	37 °C
[Ref.: #59961]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16819]	Oxygen tolerance	anaerobe
[Ref.: #32204]	Oxygen tolerance	anaerobe
[Ref.: #59961]	Oxygen tolerance	anaerobe

[Ref.: #32204]	Ability of spore formation	no
[Ref.: #68367]	Ability of spore formation	no * from API 20A
[Ref.: #69480]	Ability of spore formation	no Confidence in %92.33
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68367]	cellobiose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	D-xylose ChEBI	-	builds acid from	* from API 20A
[Ref.: #32204]	esculin ChEBI	+	hydrolysis
[Ref.: #68367]	esculin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #68367]	gelatin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #32204]	glucose ChEBI	+	carbon source
[Ref.: #68367]	L-arabinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68367]	L-rhamnose ChEBI	-	builds acid from	* from API 20A
[Ref.: #32204]	lactose ChEBI	+	carbon source
[Ref.: #32204]	maltose ChEBI	+	carbon source
[Ref.: #68367]	maltose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68367]	raffinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	salicin ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	tryptophan ChEBI	-	energy source	* from API 20A, API rID32A
[Ref.: #68367]	urea ChEBI	-	hydrolysis	* from API 20A, API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68367]		indole ChEBI	no	* from API 20A, API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A, API 20A

	Enzyme	Enzyme activity	EC number
[Ref.: #32204]	acid phosphatase	+	3.1.3.2
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #32204]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A, API 20A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #16819]	catalase	-	1.11.1.6
[Ref.: #68367]	catalase	-	1.11.1.6	* from API 20A
[Ref.: #16819]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68367]	gelatinase	-		* from API 20A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API 20A
	Only first 10 entries are displayed. Click here to see all.

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #16819]	2147	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16819]	2082	-	-	+/-	-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #16819]	12957	-	-	-	+	+/-	+	-	-	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	+	-
[Ref.: #16819]	12427	-	-	-	+	+	+	-	-	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	not determinedn.d.	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16819]	Sample type/isolated from	amniotic fluid of a 29-year-old female
[Ref.: #16819]	Geographic location (country and/or sea, region)	Göteborg
[Ref.: #16819]	Country	Sweden
[Ref.: #16819]	Country ISO 3 Code	SWE
[Ref.: #16819]	Continent	Europe

[Ref.: #59961]	Sample type/isolated from	Human amniotic fluid,turbid and ill smelling
[Ref.: #59961]	Sampling date	2006-10-23
[Ref.: #59961]	Geographic location (country and/or sea, region)	Göteborg
[Ref.: #59961]	Country	Sweden
[Ref.: #59961]	Country ISO 3 Code	SWE
[Ref.: #59961]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Human amniotic fluid
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host Body Product	#Fluids	-
#Host	#Human	#Female

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM422125 (>99% sequence identity) for Prevotella amnii subclade from Microbeatlas

Aquatic Samples	589
Soil Samples	340
Animal Samples	40800
Plant Samples	149
Total Samples	41878

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16819]	Pathogenicity (human)	yes, in single cases
[Ref.: #16819]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16819]	Prevotella amniotica partial 16S rRNA gene, type strain CCUG 53648	AM422125	1474	GenBank ENA	419005 tax ID
[Ref.: #20218]	Prevotella amnii gene for 16S ribosomal RNA, partial sequence, strain: JCM 14753	AB547670	1491	GenBank ENA	419005 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Prevotella amnii DSM 23384 = JCM 14753	GCA_000378745	scaffold	GenBank ENA	1122979 tax ID
[Ref.: #67770]	Prevotella amnii DSM 23384 = JCM 14753	GCA_000613765	contig	GenBank ENA	1122979 tax ID
[Ref.: #66792]	Prevotella amnii DSM 23384	1122979.3	wgs	PATRIC	1122979 tax ID	*
[Ref.: #66792]	Prevotella amnii JCM 14753	1236496.3	wgs	PATRIC	1122979 tax ID	*
[Ref.: #66792]	Prevotella amnii JCM 14753	2585427892	draft	JGI IMG/M	1122979 tax ID	*
[Ref.: #66792]	Prevotella amnii DSM 23384	2518645615	draft	JGI IMG/M	1122979 tax ID	*

[Ref.: #16819]

GC-content

36.6 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Prevotella amnii JCM 14753 PATRIC: 1236496.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella amnii DSM 23384 PATRIC: 1122979.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	95.734	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	88.281	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.33	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	96.58	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.083	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90.5	no

Availability in culture collections External links

[Ref.: #16819]

Culture collection no.

DSM 23384, CCUG 53648, JCM 14753, KCTC 15072

[Ref.: #81776]

SI-ID 303425

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Prevotella amnii sp. nov., isolated from human amniotic fluid.	Lawson PA, Moore E, Falsen E	Int J Syst Evol Microbiol	10.1099/ijs.0.65118-0	2008	*

References

#16819

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23384

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32204

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28446 (see below)

#59961 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 53648

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68367

Automatically annotated from API 20A .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81776

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID303425.1 )

#28446

IJSEM 89 2008 ( DOI 10.1099/ijs.0.65118-0 , PubMed 18175689 )

* These data were automatically processed and therefore are not curated

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Search for species Prevotella amnii in external resources:
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Prevotella amnii JCM 14753

Prevotella amnii DSM 23384

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