Name: Actinobacillus equuli PM 30/53
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11689

Type strain:

Species: Actinobacillus equuli

Strain Designation: PM 30/53

Culture col. no.: ATCC 19392, MCCM 00142, DSM 19655, CCUG 2041, CIP 103284, JCM 2432, NCTC 8529, LMG 3736

Strain history: CIP <- 1989, NCTC <- 1953, W.C. Miller: strain PM30/53(d108), Bacillus viscosum-equi

NCBI tax ID(s): 718 (species)

SI-ID 92092

LMG 3736, ATCC 19392, CCUG 2041, JCM 2432, NCTC 8529, CIP 103284, DSM 19655

Other strains from Actinobacillus equuli

A. equuli V1, CIP 63.55
A. equuli CIP 54.78, NCTC 3365
A. equuli G, CIP 54.48
A. equuli C, CIP 54.47
A. equuli B, CIP 54.46
A. equuli V10, CIP 63.56
A. equuli CCUG 18144
A. equuli CCUG 18724
A. equuli CCUG 18725
A. equuli CCUG 18726
A. equuli CCUG 27337, CG 592/88
A. equuli CCUG 27338
A. equuli CCUG 27340, CG 546/89a
A. equuli CCUG 27345, CG 558/90
A. equuli CCUG 27372
A. equuli CCUG 37043, MCCM 02226
A. equuli CCUG 39615
A. equuli CCUG 42295

Section

Actinobacillus equuli PM 30/53 is a mesophilic, Gram-negative, rod-shaped human pathogen that was isolated from horse blood; foal that died of sleepy-foal disease.

Gram-negative
rod-shaped
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Actinobacillus*
	Species	**Actinobacillus equuli**
	Full Scientific Name (LPSN)	Actinobacillus equuli (van Straaten 1918) Haupt 1934 (Approved Lists 1980)

Synonym

"Bacillus equuli"

Information on morphological and physiological properties Morphology

[Ref.: #119128]	Gram stain	negative

[Ref.: #119128]	Cell shape	rod-shaped

[Ref.: #119128]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8234]

Culture medium

COLUMBIA BLOOD AGAR (DSMZ Medium 429)

[Ref.: #8234]

Culture medium growth

[Ref.: #8234]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8234]

Culture medium composition

expand / minimize

[Ref.: #40587]

Culture medium

MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serum

[Ref.: #40587]

Culture medium growth

[Ref.: #40587]

Culture medium composition

expand / minimize

[Ref.: #119128]

Culture medium

CIP Medium 4

[Ref.: #119128]

Culture medium growth

[Ref.: #119128]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8234]	growth	37 °C
[Ref.: #40587]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #8234]	Temperature range	mesophilic
[Ref.: #40587]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E, API ID32E
[Ref.: #68374]	cellobiose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68374]	D-arabitol ChEBI	-	builds acid from	* from API ID32E, API rID32STR
[Ref.: #68377]	D-fructose ChEBI	+	builds acid from	* from API NH
[Ref.: #68374]	D-galacturonic acid ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68377]	D-glucose ChEBI	+	builds acid from	* from API NH
[Ref.: #68374]	D-glucose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	D-glucose ChEBI	-	fermentation	* from API 20E
[Ref.: #68374]	D-mannitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68374]	L-arabinose ChEBI	-	builds acid from	* from API ID32E, API rID32STR
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68374]	L-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	L-rhamnose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E, API ID32E
[Ref.: #68374]	malonate ChEBI	-	assimilation	* from API ID32E
[Ref.: #68377]	maltose ChEBI	+	builds acid from	* from API NH, API rID32STR
[Ref.: #68374]	maltose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68374]	myo-inositol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E, API ID32E
[Ref.: #68374]	palatinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68374]	ribitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	sorbitol ChEBI	-	builds acid from	* from API ID32E, API rID32STR
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68374]	sucrose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68374]	trehalose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E, API ID32E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E, API ID32E, API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E, API rID32STR
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68374]	indole ChEBI	no	* from API ID32E, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68381]		acetoin ChEBI	-		* from API rID32STR, API 20E
[Ref.: #68374]		indole ChEBI		-	* from API ID32E, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	+	3.1.3.1	* from API rID32STR, API NH
[Ref.: #68374]	alpha-galactosidase	+	3.2.1.22	* from API ID32E
[Ref.: #68374]	alpha-glucosidase	-	3.2.1.20	* from API ID32E
[Ref.: #68374]	alpha-maltosidase	-		* from API ID32E
[Ref.: #68374]	arginine dihydrolase	-	3.5.3.6	* from API ID32E, API 20E
[Ref.: #68374]	beta-galactosidase	+	3.2.1.23	* from API ID32E, API 20E
[Ref.: #68381]	beta-glucosidase	-	3.2.1.21	* from API rID32STR, API ID32E
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR, API ID32E
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #68368]	cytochrome oxidase	+	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68381]	glycyl tryptophan arylamidase	-		* from API rID32STR
[Ref.: #68374]	L-aspartate arylamidase	-	3.4.11.21	* from API ID32E
[Ref.: #68377]	lipase	+		* from API NH, API ID32E
[Ref.: #68374]	lysine decarboxylase	-	4.1.1.18	* from API ID32E, API 20E
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR, API ID32E
[Ref.: #68374]	ornithine decarboxylase	-	4.1.1.17	* from API ID32E, API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68381]	urease	+	3.5.1.5	* from API rID32STR, API ID32E, API 20E
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44468]

Fatty acid	Percentage	ECL
C16:1 ω7c	38.3	15.819
C14:0	27.7	14
C16:0	20.6	16
C14:0 3OH/C16:1 ISO I	9.3	15.485
C12:0	1.5	12
C18:2 ω6,9c/C18:0 ANTE	1.2	17.724
C13:0 ISO 2OH	0.8	13.814
unknown 14.503	0.6	14.503

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #44468]	5940	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® ID32E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Ornithine decarboxylaseODC	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Urease/urea hydrolysisURE	Acid from L-arabitolLARL	Acid from D-galacturonic acidGAT	Acid from 5-Ketogluconate5KG	LipaseLIP	Phenol red (Acidification)RP	beta-Glucosidasebeta GLU	Acid from D-mannitolMAN	Acid from maltoseMAL	Acid from D-adonitolADO	Acid from palatinosePLE	beta-Glucuronidasebeta GUR	MalonateMNT	Indole productionIND	N-Acetyl-beta-Glucosaminidasebeta NAG	beta-Galactosidasebeta GAL	Acid from D-glucoseGLU	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	alpha-Glucosidasealpha GLU	alpha-Galactosidasealpha GAL	Acid from trehaloseTRE	Acid from L-rhamnoseRHA	Acid from inositolINO	Acid from cellobioseCEL	Acid from sorbitolSOR	alpha-MaltosidasealphaMAL	L-aspartic acid arylamidaseAspA
[Ref.: #44468]	757	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #8234]	6125	+	+	+	+	-	-	+	+	+	-	+	-	+
[Ref.: #8234]	6124	+	+	+	+	-	+	+	+	+	-	+	-	-
[Ref.: #8234]	5748	-	+	+	+	+	-	+	+	+	+	-	+	-
[Ref.: #8234]	5747	+	+	+	+	+	-	-	+	+	+	-	+	-

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #8234]	10373	+	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	+/-	-	-	+	-	-	+	-	-	-	-	-	+
[Ref.: #8234]	10372	-	-	+	-	+	+	+	+	-	+	+	+	+	-	-	-	-	-	+	-	-	-	-	-	-	+	+/-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8234]	Sample type/isolated from	horse blood; foal that died of sleepy-foal disease
[Ref.: #8234]	Country	United Kingdom
[Ref.: #8234]	Country ISO 3 Code	GBR
[Ref.: #8234]	Continent	Europe

[Ref.: #44468]	Sample type/isolated from	Foal blood
[Ref.: #44468]	Sampling date	1953
[Ref.: #44468]	Geographic location (country and/or sea, region)	Newmarket
[Ref.: #44468]	Country	United Kingdom
[Ref.: #44468]	Country ISO 3 Code	GBR
[Ref.: #44468]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Blood of foal dead from "Sleepy-foal Disease"

[Ref.: #119128]	Sample type/isolated from	Animal, Foal, blood
[Ref.: #119128]	Isolation date	1953
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Equidae (Horse)
#Host Body Product	#Fluids	#Blood

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF381186 (>99% sequence identity) for Actinobacillus from Microbeatlas

Aquatic Samples	1
Soil Samples
Animal Samples	16
Plant Samples
Total Samples	17

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8234]	Pathogenicity (human)	yes
[Ref.: #8234]	Pathogenicity (animal)	yes
[Ref.: #8234]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119128]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8234]	Actinobacillus equuli 16S ribosomal RNA gene, partial sequence	AF381186	1362	ENA	202947 tax ID
[Ref.: #20218]	Actinobacillus equuli partial 16S rRNA gene, strain P157	AJ438649	435	ENA	718 tax ID	*
[Ref.: #20218]	Actinobacillus equuli strain NCTC 8529 16S ribosomal RNA gene, partial sequence	M75072	1477	ENA	718 tax ID	*
[Ref.: #20218]	A.equuli 16S rRNA, V3-V4-V5 variable regions, segment 1	M23308	100	ENA	718 tax ID	*
[Ref.: #20218]	A.equuli 16S rRNA, V3-V4-V5 variable regions, segment 2	M23309	345	ENA	718 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinobacillus equuli NCTC8529	GCA_900636745	complete	GenBank	718 tax ID	*
[Ref.: #66792]	Actinobacillus equuli strain NCTC8529	718.4	complete	PATRIC	718 tax ID	*
[Ref.: #66792]	Actinobacillus equuli subsp. equuli CCUG2041	GCA_029773685	complete	GenBank	202947 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinobacillus equuli NCTC8529 NCBI: GCA_900636745.1
[Ref.: #69480]	flagellated	no	93.569	no
[Ref.: #69480]	gram-positive	no	97.965	no
[Ref.: #69480]	anaerobic	no	97.969	no
[Ref.: #69480]	aerobic	no	84.272	no
[Ref.: #69480]	halophile	no	55.866	no
[Ref.: #69480]	spore-forming	no	98.539	no
[Ref.: #69480]	thermophile	no	98.988	yes
[Ref.: #69480]	glucose-util	no	56.447	no
[Ref.: #69480]	motile	no	90.286	no
[Ref.: #69480]	glucose-ferment	no	56.162	no

Availability in culture collections External links

[Ref.: #8234]

Culture collection no.

ATCC 19392, MCCM 00142, DSM 19655, CCUG 2041, CIP 103284, JCM 2432, NCTC 8529, LMG 3736

[Ref.: #80909]

SI-ID 92092

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of Actinobacillus equuli subspecies equuli ATCC 19392(T).	Huang BF, Kropinski AM, Bujold AR, MacInnes JI	Stand Genomic Sci	10.1186/s40793-015-0009-x	2015	*
Phylogeny	Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or Bisgaard taxon 11 and proposal of A. equuli subsp. equuli subsp. nov. and A. equuli subsp. haemolyticus subsp. nov.	Christensen H, Bisgaard M, Olsen JE	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1569	2002	*

References

#8234

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19655

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40587 ; Curators of the CIP;
#44468 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 2041

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68374

Automatically annotated from API ID32E .

#68377

Automatically annotated from API NH .

#68381

Automatically annotated from API rID32STR .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#80909

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92092.1 )

#119128 Collection of Institut Pasteur ; Curators of the CIP; CIP 103284
* These data were automatically processed and therefore are not curated

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Search for species Actinobacillus equuli subsp. equuli in external resources:
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Actinobacillus equuli NCTC8529

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Change proposal