Strain identifier
BacDive ID: 11689
Type strain:
Species: Actinobacillus equuli
Strain Designation: PM 30/53
Strain history: CIP <- 1989, NCTC <- 1953, W.C. Miller: strain PM30/53(d108), Bacillus viscosum-equi
NCBI tax ID(s): 718 (species)
General
@ref: 8234
BacDive-ID: 11689
DSM-Number: 19655
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped, human pathogen
description: Actinobacillus equuli PM 30/53 is a mesophilic, Gram-negative, rod-shaped human pathogen that was isolated from horse blood; foal that died of sleepy-foal disease.
NCBI tax id
- NCBI tax id: 718
- Matching level: species
strain history
@ref | history |
---|---|
8234 | <- JCM <- R. Sakazaki <- ATCC <- NCTC <- W. C. Miller; PM 30/53 (Bacillus viscosum-equi) |
67770 | R. Sakazaki <-- ATCC 19392 <-- NCTC 8529 <-- W. C. Miller (PM30/53, C.108). |
119128 | CIP <- 1989, NCTC <- 1953, W.C. Miller: strain PM30/53(d108), Bacillus viscosum-equi |
doi: 10.13145/bacdive11689.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pasteurellales
- family: Pasteurellaceae
- genus: Actinobacillus
- species: Actinobacillus equuli
- full scientific name: Actinobacillus equuli (van Straaten 1918) Haupt 1934 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacillus equuli
@ref: 8234
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pasteurellales
family: Pasteurellaceae
genus: Actinobacillus
species: Actinobacillus equuli subsp. equuli
full scientific name: Actinobacillus equuli subsp. equuli (van Straaten 1918) Christensen et al. 2002
strain designation: PM 30/53
type strain: yes
Morphology
cell morphology
- @ref: 119128
- gram stain: negative
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8234 | COLUMBIA BLOOD AGAR (DSMZ Medium 429) | yes | https://mediadive.dsmz.de/medium/429 | Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429; with strain-specific modifications) Composition: Horse serum 200.0 g/l Horse blood 40.0 g/l Columbia agar base |
40587 | MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serum | yes | Horse serum (200.000 ml);Columbia agar (39.000 g);Distilled water make up to (1000.000 ml) | |
119128 | CIP Medium 4 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=4 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8234 | positive | growth | 37 | mesophilic |
40587 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68368 | 30849 | L-arabinose | - | fermentation |
68368 | 27613 | amygdalin | - | fermentation |
68368 | 28053 | melibiose | - | fermentation |
68368 | 17992 | sucrose | - | fermentation |
68368 | 62345 | L-rhamnose | - | fermentation |
68368 | 30911 | sorbitol | - | fermentation |
68368 | 17268 | myo-inositol | - | fermentation |
68368 | 16899 | D-mannitol | - | fermentation |
68368 | 17634 | D-glucose | - | fermentation |
68368 | 5291 | gelatin | - | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | + | hydrolysis |
68368 | 16947 | citrate | - | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | - | hydrolysis |
68374 | 18257 | ornithine | - | degradation |
68374 | 29016 | arginine | - | hydrolysis |
68374 | 25094 | lysine | - | degradation |
68374 | 16199 | urea | + | hydrolysis |
68374 | 18403 | L-arabitol | - | builds acid from |
68374 | 18024 | D-galacturonic acid | - | builds acid from |
68374 | Potassium 5-ketogluconate | - | builds acid from | |
68374 | 16899 | D-mannitol | - | builds acid from |
68374 | 17306 | maltose | - | builds acid from |
68374 | 15963 | ribitol | - | builds acid from |
68374 | 18394 | palatinose | - | builds acid from |
68374 | 15792 | malonate | - | assimilation |
68374 | 27897 | tryptophan | - | energy source |
68374 | 17634 | D-glucose | - | builds acid from |
68374 | 17992 | sucrose | - | builds acid from |
68374 | 30849 | L-arabinose | - | builds acid from |
68374 | 18333 | D-arabitol | - | builds acid from |
68374 | 27082 | trehalose | - | builds acid from |
68374 | 62345 | L-rhamnose | - | builds acid from |
68374 | 17268 | myo-inositol | - | builds acid from |
68374 | 17057 | cellobiose | - | builds acid from |
68374 | 30911 | sorbitol | - | builds acid from |
68377 | 17634 | D-glucose | + | builds acid from |
68377 | 15824 | D-fructose | + | builds acid from |
68377 | 17306 | maltose | + | builds acid from |
68381 | 30911 | sorbitol | - | builds acid from |
68381 | 17992 | sucrose | + | builds acid from |
68381 | 30849 | L-arabinose | - | builds acid from |
68381 | 18333 | D-arabitol | - | builds acid from |
68381 | 40585 | alpha-cyclodextrin | - | builds acid from |
68381 | 28087 | glycogen | - | builds acid from |
68381 | 27941 | pullulan | - | builds acid from |
68381 | 17306 | maltose | + | builds acid from |
68381 | 6731 | melezitose | - | builds acid from |
68381 | 320055 | methyl beta-D-glucopyranoside | - | builds acid from |
68381 | 16443 | D-tagatose | - | builds acid from |
68381 | 16199 | urea | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68374 | 35581 | indole | no |
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
68381 | 15688 | acetoin | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68381 | 15688 | acetoin | - | |
68374 | 35581 | indole | - | |
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68381 | urease | + | 3.5.1.5 |
68381 | beta-mannosidase | - | 3.2.1.25 |
68381 | glycyl tryptophan arylamidase | - | |
68381 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | |
68381 | alkaline phosphatase | + | 3.1.3.1 |
68381 | beta-glucuronidase | - | 3.2.1.31 |
68381 | beta-glucosidase | - | 3.2.1.21 |
68377 | alkaline phosphatase | + | 3.1.3.1 |
68377 | lipase | + | |
68374 | L-aspartate arylamidase | - | 3.4.11.21 |
68374 | alpha-maltosidase | - | |
68374 | alpha-galactosidase | + | 3.2.1.22 |
68374 | alpha-glucosidase | - | 3.2.1.20 |
68374 | beta-galactosidase | + | 3.2.1.23 |
68374 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68374 | beta-glucuronidase | - | 3.2.1.31 |
68374 | beta-glucosidase | - | 3.2.1.21 |
68374 | lipase | + | |
68374 | urease | + | 3.5.1.5 |
68374 | lysine decarboxylase | - | 4.1.1.18 |
68374 | arginine dihydrolase | - | 3.5.3.6 |
68374 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | cytochrome oxidase | + | 1.9.3.1 |
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | + | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 44468 C12:0 1.5 12 44468 C14:0 27.7 14 44468 C16:0 20.6 16 44468 C13:0 ISO 2OH 0.8 13.814 44468 C14:0 3OH/C16:1 ISO I 9.3 15.485 44468 C16:1 ω7c 38.3 15.819 44468 C18:2 ω6,9c/C18:0 ANTE 1.2 17.724 44468 unknown 14.503 0.6 14.503 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
44468 | + | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + |
API ID32E
@ref | ODC | ADH Arg | LDC Lys | URE | LARL | GAT | 5KG | LIP | RP | beta GLU | MAN | MAL | ADO | PLE | beta GUR | MNT | IND | beta NAG | beta GAL | GLU | SAC | LARA | DARL | alpha GLU | alpha GAL | TRE | RHA | INO | CEL | SOR | alphaMAL | AspA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
44468 | - | - | - | + | - | - | - | + | + | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | + | - | - | - | - | - | - | - |
API NH
@ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8234 | + | + | + | + | - | - | + | + | + | - | + | - | + |
8234 | + | + | + | + | - | + | + | + | + | - | + | - | - |
8234 | - | + | + | + | + | - | + | + | + | + | - | + | - |
8234 | + | + | + | + | + | - | - | + | + | + | - | + | - |
API rID32STR
@ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8234 | + | - | - | - | - | + | - | - | - | - | - | - | + | - | - | - | - | - | - | +/- | - | - | + | - | - | + | - | - | - | - | - | + |
8234 | - | - | + | - | + | + | + | + | - | + | + | + | + | - | - | - | - | - | + | - | - | - | - | - | - | + | +/- | - | - | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | sampling date | geographic location | isolation date |
---|---|---|---|---|---|---|---|
8234 | horse blood; foal that died of sleepy-foal disease | United Kingdom | GBR | Europe | |||
44468 | Foal blood | United Kingdom | GBR | Europe | 1953 | Newmarket | |
67770 | Blood of foal dead from "Sleepy-foal Disease" | ||||||
119128 | Animal, Foal, blood | 1953 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Equidae (Horse) |
#Host Body Product | #Fluids | #Blood |
taxonmaps
- @ref: 69479
- File name: preview.99_171678.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_155;97_166;98_344;99_171678&stattab=map
- Last taxonomy: Actinobacillus
- 16S sequence: AF381186
- Sequence Identity:
- Total samples: 17
- aquatic counts: 1
- animal counts: 16
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
8234 | yes | yes | 2 | Risk group (German classification) |
119128 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Actinobacillus equuli partial 16S rRNA gene, strain P157 | AJ438649 | 435 | ena | 718 |
20218 | A.equuli 16S rRNA, V3-V4-V5 variable regions, segment 1 | M23308 | 100 | ena | 718 |
20218 | A.equuli 16S rRNA, V3-V4-V5 variable regions, segment 2 | M23309 | 345 | ena | 718 |
20218 | Actinobacillus equuli strain NCTC 8529 16S ribosomal RNA gene, partial sequence | M75072 | 1477 | ena | 718 |
8234 | Actinobacillus equuli 16S ribosomal RNA gene, partial sequence | AF381186 | 1362 | ena | 202947 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Actinobacillus equuli NCTC8529 | GCA_900636745 | complete | ncbi | 718 |
66792 | Actinobacillus equuli strain NCTC8529 | 718.4 | complete | patric | 718 |
66792 | Actinobacillus equuli subsp. equuli CCUG2041 | GCA_029773685 | complete | ncbi | 202947 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 93.569 | no |
gram-positive | no | 97.965 | no |
anaerobic | no | 97.969 | no |
aerobic | no | 84.272 | no |
halophile | no | 55.866 | no |
spore-forming | no | 98.539 | no |
thermophile | no | 98.988 | yes |
glucose-util | no | 56.447 | no |
motile | no | 90.286 | no |
glucose-ferment | no | 56.162 | no |
External links
@ref: 8234
culture collection no.: ATCC 19392, MCCM 00142, DSM 19655, CCUG 2041, CIP 103284, JCM 2432, NCTC 8529, LMG 3736
straininfo link
- @ref: 80909
- straininfo: 92092
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 12361259 | Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or Bisgaard taxon 11 and proposal of A. equuli subsp. equuli subsp. nov. and A. equuli subsp. haemolyticus subsp. nov. | Christensen H, Bisgaard M, Olsen JE | Int J Syst Evol Microbiol | 10.1099/00207713-52-5-1569 | 2002 | Actinobacillus/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Horses/*microbiology, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic | Pathogenicity |
Genetics | 26203343 | Complete genome sequence of Actinobacillus equuli subspecies equuli ATCC 19392(T). | Huang BF, Kropinski AM, Bujold AR, MacInnes JI | Stand Genomic Sci | 10.1186/s40793-015-0009-x | 2015 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8234 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19655) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19655 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40587 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15064 | ||||
44468 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 2041) | https://www.ccug.se/strain?id=2041 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
68374 | Automatically annotated from API ID32E | |||||
68377 | Automatically annotated from API NH | |||||
68381 | Automatically annotated from API rID32STR | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
80909 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92092.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119128 | Curators of the CIP | Collection of Institut Pasteur (CIP 103284) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103284 |