Strain identifier

BacDive ID: 11689

Type strain: Yes

Species: Actinobacillus equuli

Strain Designation: PM 30/53

Strain history: CIP <- 1989, NCTC <- 1953, W.C. Miller: strain PM30/53(d108), Bacillus viscosum-equi

NCBI tax ID(s): 718 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8234

BacDive-ID: 11689

DSM-Number: 19655

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Actinobacillus equuli PM 30/53 is a mesophilic, Gram-negative, rod-shaped human pathogen that was isolated from horse blood; foal that died of sleepy-foal disease.

NCBI tax id

  • NCBI tax id: 718
  • Matching level: species

strain history

@refhistory
8234<- JCM <- R. Sakazaki <- ATCC <- NCTC <- W. C. Miller; PM 30/53 (Bacillus viscosum-equi)
67770R. Sakazaki <-- ATCC 19392 <-- NCTC 8529 <-- W. C. Miller (PM30/53, C.108).
119128CIP <- 1989, NCTC <- 1953, W.C. Miller: strain PM30/53(d108), Bacillus viscosum-equi

doi: 10.13145/bacdive11689.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus equuli
  • full scientific name: Actinobacillus equuli (van Straaten 1918) Haupt 1934 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus equuli

@ref: 8234

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus equuli subsp. equuli

full scientific name: Actinobacillus equuli subsp. equuli (van Straaten 1918) Christensen et al. 2002

strain designation: PM 30/53

type strain: yes

Morphology

cell morphology

  • @ref: 119128
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8234COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429; with strain-specific modifications) Composition: Horse serum 200.0 g/l Horse blood 40.0 g/l Columbia agar base
40587MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serumyesHorse serum (200.000 ml);Columbia agar (39.000 g);Distilled water make up to (1000.000 ml)
119128CIP Medium 4yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=4

culture temp

@refgrowthtypetemperaturerange
8234positivegrowth37mesophilic
40587positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
6837418257ornithine-degradation
6837429016arginine-hydrolysis
6837425094lysine-degradation
6837416199urea+hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid-builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol-builds acid from
6837417306maltose-builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan-energy source
6837417634D-glucose-builds acid from
6837417992sucrose-builds acid from
6837430849L-arabinose-builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose-builds acid from
6837462345L-rhamnose-builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from
6837717634D-glucose+builds acid from
6837715824D-fructose+builds acid from
6837717306maltose+builds acid from
6838130911sorbitol-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin-
6837435581indole-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68381urease+3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase+3.1.3.1
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68377alkaline phosphatase+3.1.3.1
68377lipase+
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase-
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase+
68374urease+3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase-4.1.1.17
68368cytochrome oxidase+1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44468C12:01.512
    44468C14:027.714
    44468C16:020.616
    44468C13:0 ISO 2OH0.813.814
    44468C14:0 3OH/C16:1 ISO I9.315.485
    44468C16:1 ω7c38.315.819
    44468C18:2 ω6,9c/C18:0 ANTE1.217.724
    44468unknown 14.5030.614.503
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
44468+-----+-------------+

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
44468---+---++---------+-----+-------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
8234++++--+++-+-+
8234++++-++++-+--
8234-++++-++++-+-
8234+++++--+++-+-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8234+----+------+------+/---+--+-----+
8234--+-++++-++++-----+------++/-----+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationisolation date
8234horse blood; foal that died of sleepy-foal diseaseUnited KingdomGBREurope
44468Foal bloodUnited KingdomGBREurope1953Newmarket
67770Blood of foal dead from "Sleepy-foal Disease"
119128Animal, Foal, blood1953

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_171678.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_155;97_166;98_344;99_171678&stattab=map
  • Last taxonomy: Actinobacillus
  • 16S sequence: AF381186
  • Sequence Identity:
  • Total samples: 17
  • aquatic counts: 1
  • animal counts: 16

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8234yesyes2Risk group (German classification)
1191281Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus equuli partial 16S rRNA gene, strain P157AJ438649435ena718
20218A.equuli 16S rRNA, V3-V4-V5 variable regions, segment 1M23308100ena718
20218A.equuli 16S rRNA, V3-V4-V5 variable regions, segment 2M23309345ena718
20218Actinobacillus equuli strain NCTC 8529 16S ribosomal RNA gene, partial sequenceM750721477ena718
8234Actinobacillus equuli 16S ribosomal RNA gene, partial sequenceAF3811861362ena202947

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinobacillus equuli NCTC8529GCA_900636745completencbi718
66792Actinobacillus equuli strain NCTC8529718.4completepatric718
66792Actinobacillus equuli subsp. equuli CCUG2041GCA_029773685completencbi202947

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno93.569no
gram-positiveno97.965no
anaerobicno97.969no
aerobicno84.272no
halophileno55.866no
spore-formingno98.539no
thermophileno98.988yes
glucose-utilno56.447no
motileno90.286no
glucose-fermentno56.162no

External links

@ref: 8234

culture collection no.: ATCC 19392, MCCM 00142, DSM 19655, CCUG 2041, CIP 103284, JCM 2432, NCTC 8529, LMG 3736

straininfo link

  • @ref: 80909
  • straininfo: 92092

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12361259Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or Bisgaard taxon 11 and proposal of A. equuli subsp. equuli subsp. nov. and A. equuli subsp. haemolyticus subsp. nov.Christensen H, Bisgaard M, Olsen JEInt J Syst Evol Microbiol10.1099/00207713-52-5-15692002Actinobacillus/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Horses/*microbiology, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as TopicPathogenicity
Genetics26203343Complete genome sequence of Actinobacillus equuli subspecies equuli ATCC 19392(T).Huang BF, Kropinski AM, Bujold AR, MacInnes JIStand Genomic Sci10.1186/s40793-015-0009-x2015

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8234Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19655)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19655
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40587Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15064
44468Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2041)https://www.ccug.se/strain?id=2041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68374Automatically annotated from API ID32E
68377Automatically annotated from API NH
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80909Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92092.1StrainInfo: A central database for resolving microbial strain identifiers
119128Curators of the CIPCollection of Institut Pasteur (CIP 103284)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103284