Actinobacillus porcinus (DSM 22435, CCUG 38924, CIP 105315)

Name: Actinobacillus porcinus (DSM 22435, CCUG 38924, CIP 105315)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11684

Type strain

Strain Designation NM319, NM 319

Culture col. no. DSM 22435 CCUG 38924 CIP 105315 NM319 NM 319

NCBI tax ID(s) 51048

Special Collections DSMZ CCUG CIP

History <- CCUG; NM319 <- K. Möller, Danish Vet. Lab., Copenhagen, Denmark <- S. Rosendal, Univ. Guelph, Ontario, Canada CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain NM 319 <- K. Moeller <- S. Rosendal

Links

Actinobacillus porcinus NM319 is a microaerophile, mesophilic, Gram-negative prokaryote that was isolated from pig upper respiratory tract.

Gram-negative rod-shaped microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 51123

CCUG 38924,CIP 105315,DSM 22435

@ref 20215

Last LPSN update 2026-02-09 11:20:37

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pasteurellales

Family Pasteurellaceae

Genus Actinobacillus

Species Actinobacillus porcinus

Full scientific name Actinobacillus porcinus Møller et al. 1996

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121944	negative	rod-shaped
125438			96.321
125438	negative		96.5

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16331	HAEMOPHILUS MEDIUM (DSMZ Medium 804)	Medium recipe at MediaDive	Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
39259	MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
121944	CIP Medium 10	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16331	positive	growth	37	mesophilic
39259	positive	growth	37	mesophilic
54557	positive	growth	37	mesophilic
121944	positive	growth	25-41
121944	negative	growth	15
121944	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
16331	microaerophile
16331	aerobe
54557	microaerophile
121944	facultative anaerobe
125439	microaerophile	97.6

Spore formation

@ref	Spore formation	Confidence
125439		99.1
125438		94.832

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68377	15824 ChEBI	D-fructose	+	builds acid from	from API NH
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68377	17634 ChEBI	D-glucose	+	builds acid from	from API NH
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	+	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
121944	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
121944	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68377	17306 ChEBI	maltose	+	builds acid from	from API NH
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
121944	17632 ChEBI	nitrate	+	reduction
121944	16301 ChEBI	nitrite	-	reduction
68377	18257 ChEBI	ornithine	-	degradation	from API NH
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68377	27897 ChEBI	tryptophan	-	energy source	from API NH
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68377	16199 ChEBI	urea	-	hydrolysis	from API NH
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
121944	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68377	35581 ChEBI	indole		from API NH
121944	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68377	35581 ChEBI	indole	-	from API NH

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121944	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	+	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
121944	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
16331	catalase	+	1.11.1.6
121944	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
16331	cytochrome-c oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
121944	gamma-glutamyltransferase	-	2.3.2.2
68382	leucine arylamidase	-	3.4.11.1	from API zym
68377	lipase	-		from API NH
68382	lipase (C 14)	-		from API zym
121944	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
121944	ornithine decarboxylase	-	4.1.1.17
68377	ornithine decarboxylase	-	4.1.1.17	from API NH
121944	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68377	tryptophan deaminase	-	4.1.99.1	from API NH
121944	urease	-	3.5.1.5
68377	urease	-	3.5.1.5	from API NH
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121944	-	+	+	-	-	-	-	-	-	-	+	-	+	+	-	-	-	-	-	+

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
121944	not determinedn.d.	+	-	-	-	+	+	-	-	-	-	+	+	+	-	-	-	-	+	+	-	-	+/-	+/-	+	+	+/-	+/-	+	+	+	+	+	-	-	+	-	+/-	-	-	-	-	-	-	-	-	-	+	-	+

API NH

@ref	PEN	GLU	FRU	MAL	SAC	ODC	URE	LIP	PAL	beta GAL	ProA	GGT	IND
16331	+	+	+	+	-	-	-	-	+	-	+	-	-
16331	-	+	+	+	+	-	-	-	-	+	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body-Site	#Oral cavity and airways	#Airways

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
16331	pig upper respiratory tract	Ontario	Canada	CAN	North America
54557	Pig upper respiratory tract	Guelph	Canada	CAN	North America
121944	Animal, Pig, respiratory tract	Ontario	Canada	CAN	North America

Taxonmaps

69479

Global distribution of 16S sequence AY362896 (>99% sequence identity) for Actinobacillus porcinus subclade from Microbeatlas

Aquatic Samples	5
Soil Samples	2
Animal Samples	768
Plant Samples	4
Total Samples	779

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16331	1	Risk group (German classification) According to TRBA 466
121944	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score	BV-BRC accession
66792	5970_8#10_v2 assembly for Actinobacillus porcinus NM319	scaffold	GCA_901764995	8002809299	51048	71.51
66792	5970_8#10-2 assembly for Actinobacillus porcinus	scaffold	GCA_900773985		51048		51048.3

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16331	Actinobacillus porcinus strain CCUG 38924 16S ribosomal RNA gene, partial sequence	AY362896	1364		51048

GC content

16331

GC-content (mol%)41.4

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinobacillus porcinus NM319
NCBI: GCA_900773985

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.10	no
125439	motility	BacteriaNetⓘ	no	82.00	no
125439	gram_stain	BacteriaNetⓘ	positive	75.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	97.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinobacillus porcinus NM319
NCBI: GCA_901764995.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.50	no
125438	anaerobic	anaerobicⓘ	no	92.14	yes
125438	aerobic	aerobicⓘ	no	80.58	no
125438	spore-forming	spore-formingⓘ	no	94.83	no
125438	thermophilic	thermophileⓘ	no	97.50	yes
125438	flagellated	motile2+ⓘ	no	96.32	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Development and Validation of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Glaesserella (Haemophilus) parasuis.	Pilchova V, Seinige D, Hennig-Pauka I, Buttner K, Abdulmawjood A, Kehrenberg C.	Microorganisms	10.3390/microorganisms9010041	2020
Phylogeny	A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay.	Bosse JT, Li Y, Sarkozi R, Gottschalk M, Angen O, Nedbalcova K, Rycroft AN, Fodor L, Langford PR.	J Clin Microbiol	10.1128/jcm.02166-16	2017
Genetics	Draft Genome Sequences of the Type Strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), Two NAD-Dependent Bacterial Species Found in the Respiratory Tract of Pigs.	Bosse JT, Li Y, Fernandez Crespo R, Angen O, Holden MTG, Weinert LA, Maskell DJ, Tucker AW, Wren BW, Rycroft AN, Langford PR	Microbiol Resour Announc	10.1128/MRA.00716-19	2020
Phylogeny	Actinobacillus minor sp. nov., Actinobacillus porcinus sp. nov., and Actinobacillus indolicus sp. nov., three new V factor-dependent species from the respiratory tract of pigs.	Moller K, Fussing V, Grimont PA, Paster BJ, Dewhirst FE, Kilian M	Int J Syst Bacteriol	10.1099/00207713-46-4-951	1996

References

#16331	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22435
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39259	; Curators of the CIP;
#54557	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38924
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68377	Automatically annotated from API NH .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121944	Collection of Institut Pasteur ; Curators of the CIP; CIP 105315
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinobacillus porcinus (DSM 22435, CCUG 38924, CIP 105315)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API NH

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_901764995

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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