Name: Actinobacillus porcinus NM319
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11684

Type strain:

Species: Actinobacillus porcinus

Strain Designation: NM319, NM 319

Culture col. no.: DSM 22435, CCUG 38924, CIP 105315

Strain history: CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain NM 319 <- K. Moeller <- S. Rosendal

NCBI tax ID(s): 51048 (species)

SI-ID 51123

CCUG 38924, CIP 105315, DSM 22435

Section

Actinobacillus porcinus NM319 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from pig upper respiratory tract.

Gram-negative
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Actinobacillus*
	Species	**Actinobacillus porcinus**
	Full Scientific Name (LPSN)	Actinobacillus porcinus Møller et al. 1996

Information on morphological and physiological properties Morphology

[Ref.: #121944]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.947

[Ref.: #121944]	Cell shape	rod-shaped

[Ref.: #121944]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16331]

Culture medium

HAEMOPHILUS MEDIUM (DSMZ Medium 804)

[Ref.: #16331]

Culture medium growth

[Ref.: #16331]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16331]

Culture medium composition

expand / minimize

[Ref.: #39259]

Culture medium

MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria

[Ref.: #39259]

Culture medium growth

[Ref.: #39259]

Culture medium composition

expand / minimize

[Ref.: #121944]

Culture medium

CIP Medium 10

[Ref.: #121944]

Culture medium growth

[Ref.: #121944]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #16331]		growth	37 °C
[Ref.: #39259]		growth	37 °C
[Ref.: #54557]		growth	37 °C
[Ref.: #121944]		growth	25-41 °C
[Ref.: #121944]	no	growth	15 °C
[Ref.: #121944]	no	growth	45 °C

[Ref.: #16331]	Temperature range	mesophilic
[Ref.: #39259]	Temperature range	mesophilic
[Ref.: #54557]	Temperature range	mesophilic
[Ref.: #121944]	Temperature range	psychrophilic
[Ref.: #121944]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16331]	Oxygen tolerance	microaerophile
[Ref.: #16331]	Oxygen tolerance	aerobe
[Ref.: #54557]	Oxygen tolerance	microaerophile
[Ref.: #121944]	Oxygen tolerance	facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.984

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid, API NH
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid, API NH
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121944]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #121944]	hippurate ChEBI	-	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid, API NH
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121944]	nitrate ChEBI	+	reduction
[Ref.: #121944]	nitrite ChEBI	-	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	urea ChEBI	-	hydrolysis	* from API NH
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121944]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #121944]		indole ChEBI	no
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121944]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121944]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #16331]	catalase	+	1.11.1.6
[Ref.: #121944]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #16331]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #121944]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121944]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121944]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #121944]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #121944]	urease	-	3.5.1.5
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121944]	10036	not determinedn.d.	+	-	-	-	+	+	-	-	-	-	+	+	+	-	-	-	-	+	+	-	-	+/-	+/-	+	+	+/-	+/-	+	+	+	+	+	-	-	+	-	+/-	-	-	-	-	-	-	-	-	-	+	-	+

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #16331]	6168	+	+	+	+	-	-	-	-	+	-	+	-	-
[Ref.: #16331]	5704	-	+	+	+	+	-	-	-	-	+	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121944]	8611	-	+	+	-	-	-	-	-	-	-	+	-	+	+	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16331]	Sample type/isolated from	pig upper respiratory tract
[Ref.: #16331]	Geographic location (country and/or sea, region)	Ontario
[Ref.: #16331]	Country	Canada
[Ref.: #16331]	Country ISO 3 Code	CAN
[Ref.: #16331]	Continent	North America

[Ref.: #54557]	Sample type/isolated from	Pig upper respiratory tract
[Ref.: #54557]	Geographic location (country and/or sea, region)	Guelph
[Ref.: #54557]	Country	Canada
[Ref.: #54557]	Country ISO 3 Code	CAN
[Ref.: #54557]	Continent	North America

[Ref.: #121944]	Sample type/isolated from	Animal, Pig, respiratory tract
[Ref.: #121944]	Geographic location (country and/or sea, region)	Ontario
[Ref.: #121944]	Country	Canada
[Ref.: #121944]	Country ISO 3 Code	CAN
[Ref.: #121944]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body-Site	#Oral cavity and airways	#Airways

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY362896 (>99% sequence identity) for Actinobacillus porcinus subclade from Microbeatlas

Aquatic Samples	5
Soil Samples	2
Animal Samples	768
Plant Samples	4
Total Samples	779

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16331]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121944]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16331]	Actinobacillus porcinus strain CCUG 38924 16S ribosomal RNA gene, partial sequence	AY362896	1364	ENA	51048 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinobacillus porcinus NM319	GCA_901764995	scaffold	GenBank	51048 tax ID	*
[Ref.: #66792]	Actinobacillus porcinus NM319	GCA_900773985	scaffold	GenBank	51048 tax ID	*

[Ref.: #16331]

GC-content

41.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinobacillus porcinus NM319 NCBI: GCA_901764995.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinobacillus porcinus NM319 NCBI: GCA_901764995.1
[Ref.: #69480]	flagellated	no	95.457	no
[Ref.: #69480]	gram-positive	no	98.536	no
[Ref.: #69480]	anaerobic	no	93.808	yes
[Ref.: #69480]	aerobic	no	94.282	no
[Ref.: #69480]	halophile	no	56.177	no
[Ref.: #69480]	spore-forming	no	98.73	no
[Ref.: #69480]	thermophile	no	97.891	no
[Ref.: #69480]	glucose-util	yes	57.941	no
[Ref.: #69480]	motile	no	90.248	no
[Ref.: #69480]	glucose-ferment	yes	56.526	no

Availability in culture collections External links

[Ref.: #16331]

Culture collection no.

DSM 22435, CCUG 38924, CIP 105315

[Ref.: #80904]

SI-ID 51123

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequences of the Type Strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), Two NAD-Dependent Bacterial Species Found in the Respiratory Tract of Pigs.	Bosse JT, Li Y, Fernandez Crespo R, Angen O, Holden MTG, Weinert LA, Maskell DJ, Tucker AW, Wren BW, Rycroft AN, Langford PR	Microbiol Resour Announc	10.1128/MRA.00716-19	2020	*
Phylogeny	Actinobacillus minor sp. nov., Actinobacillus porcinus sp. nov., and Actinobacillus indolicus sp. nov., three new V factor-dependent species from the respiratory tract of pigs.	Moller K, Fussing V, Grimont PA, Paster BJ, Dewhirst FE, Kilian M	Int J Syst Bacteriol	10.1099/00207713-46-4-951	1996	*

References

#16331

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22435

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39259 ; Curators of the CIP;
#54557 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38924

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80904

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID51123.1 )

#121944 Collection of Institut Pasteur ; Curators of the CIP; CIP 105315
* These data were automatically processed and therefore are not curated

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