Name: Actinobacillus seminis 5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11683

Type strain:

Species: Actinobacillus seminis

Strain Designation: 5, A51 and A65

Culture col. no.: DSM 22263, ATCC 15768, CCUG 27187, CIP 102633, NCTC 10851, Simmons K3844-C

Strain history: <- CCUG <- NCTC <- ATCC <- G.C. Simmons

NCBI tax ID(s): 722 (species)

Other strains from Actinobacillus seminis

A. seminis CCUG 12832
A. seminis CCUG 18730
A. seminis CCUG 18731
A. seminis CCUG 23440

Section

Actinobacillus seminis 5 is a mesophilic animal pathogen that was isolated from sheep semen.

animal pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Actinobacillus*
	Species	**Actinobacillus seminis**
	Full Scientific Name (PNU)	Actinobacillus seminis (ex Baynes and Simmons 1960) Sneath and Stevens 1990

Information on morphological and physiological properties Morphology

[Ref.: #69480]

Gram stain

negative Confidence in %99.976

[Ref.: #16243]

Type of hemolysis

gamma

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16243]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #16243]

Culture medium growth

[Ref.: #16243]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #16243]

Culture medium composition

expand / minimize

[Ref.: #40386]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #40386]

Culture medium growth

[Ref.: #40386]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16243]			growth	37 °C
[Ref.: #40386]			growth	37 °C

[Ref.: #16243]	Temperature range	mesophilic
[Ref.: #40386]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.994

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68377]	D-glucose ChEBI	+	builds acid from	* from API NH
[Ref.: #68377]	maltose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68377]	urea ChEBI	-	hydrolysis	* from API NH

	Metabolite production	Metabolite
[Ref.: #68377]		indole ChEBI	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68377]	alkaline phosphatase	-	3.1.3.1	* from API NH
[Ref.: #68377]	beta-galactosidase	-	3.2.1.23	* from API NH
[Ref.: #16243]	catalase	-	1.11.1.6
[Ref.: #16243]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #16243]	5522	+	+	-	-	+	-	-	-	-	-	+	-	-
[Ref.: #16243]	5064	-	+	+	-	-	+	-	-	-	-	-	+	-

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	gluconeogenesis	100	8 of 8
[Ref.: #66794]	ubiquinone biosynthesis	100	7 of 7
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	85.71	12 of 14
[Ref.: #66794]	pentose phosphate pathway	81.82	9 of 11
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	starch degradation	80	8 of 10
[Ref.: #66794]	methylglyoxal degradation	80	4 of 5
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	pyrimidine metabolism	77.78	35 of 45
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	purine metabolism	72.34	68 of 94
[Ref.: #66794]	phenylalanine metabolism	69.23	9 of 13
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	methionine metabolism	65.38	17 of 26
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	glutathione metabolism	64.29	9 of 14
[Ref.: #66794]	proline metabolism	63.64	7 of 11
[Ref.: #66794]	urea cycle	61.54	8 of 13
[Ref.: #66794]	degradation of hexoses	61.11	11 of 18
[Ref.: #66794]	degradation of pentoses	60.71	17 of 28
[Ref.: #66794]	glutamate and glutamine metabolism	60.71	17 of 28
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	Entner Doudoroff pathway	60	6 of 10
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	heme metabolism	57.14	8 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	55.56	5 of 9
[Ref.: #66794]	non-pathway related	55.26	21 of 38
[Ref.: #66794]	isoprenoid biosynthesis	53.85	14 of 26
[Ref.: #66794]	myo-inositol biosynthesis	50	5 of 10
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	NAD metabolism	50	9 of 18
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	histidine metabolism	48.28	14 of 29
[Ref.: #66794]	lysine metabolism	47.62	20 of 42
[Ref.: #66794]	tryptophan metabolism	47.37	18 of 38
[Ref.: #66794]	oxidative phosphorylation	47.25	43 of 91
[Ref.: #66794]	leucine metabolism	46.15	6 of 13
[Ref.: #66794]	alanine metabolism	44.83	13 of 29
[Ref.: #66794]	d-mannose degradation	44.44	4 of 9
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	degradation of sugar acids	44	11 of 25
[Ref.: #66794]	cardiolipin biosynthesis	42.86	3 of 7
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	arginine metabolism	41.67	10 of 24
[Ref.: #66794]	hydrogen production	40	2 of 5
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	lipid metabolism	38.71	12 of 31
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	aclacinomycin biosynthesis	28.57	2 of 7
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	dTDPLrhamnose biosynthesis	25	2 of 8
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	sulfate reduction	23.08	3 of 13
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16243]	Sample type/isolated from	sheep semen

[Ref.: #48675]	Sample type/isolated from	Sheep semen
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body Product	#Urogenital tract	#Semen

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY362897 (>99% sequence identity) for Actinobacillus seminis subclade from Microbeatlas

Aquatic Samples	11
Soil Samples	5
Animal Samples	305
Plant Samples
Total Samples	321

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16243]	Pathogenicity (animal)	yes
[Ref.: #16243]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16243]	Actinobacillus seminis strain CCUG 27187 16S ribosomal RNA gene, partial sequence	AY362897	1364	ENA	722 tax ID
[Ref.: #20218]	Actinobacillus seminis 16S ribosomal RNA gene, partial sequence, tRNA-Glu gene, complete sequence, and 23S ribosomal RNA gene, partial sequence	AF013275	562	ENA	722 tax ID	*
[Ref.: #20218]	Actinobacillus seminis 16S ribosomal RNA gene, partial sequence, tRNA-Ile and tRNA-Ala genes, complete sequence, and 23S ribosomal RNA gene, partial sequence	AF013276	691	ENA	722 tax ID	*
[Ref.: #20218]	Actinobacillus seminis strain ATCC 15768 16S ribosomal RNA gene, partial sequence	M75047	1479	ENA	722 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinobacillus seminis NCTC10851	GCA_900460625	contig	GenBank	722 tax ID	*
[Ref.: #66792]	Actinobacillus seminis ATCC 15768	GCA_002263215	contig	GenBank	722 tax ID	*
[Ref.: #66792]	Actinobacillus seminis strain ATCC 15768	722.6	wgs	PATRIC	722 tax ID	*
[Ref.: #66792]	Actinobacillus seminis strain NCTC10851	722.7	wgs	PATRIC	722 tax ID	*
[Ref.: #66792]	Actinobacillus seminis NCTC 10851	2806310645	draft	JGI IMG/M	722 tax ID	*
[Ref.: #66792]	Actinobacillus seminis ATCC 15768	2834290966	draft	JGI IMG/M	722 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinobacillus seminis strain NCTC10851 PATRIC: 722.7
[Ref.: #69480]	spore-forming	no	99.994	yes
[Ref.: #69480]	anaerobic	no	98.606	no
[Ref.: #69480]	gram-positive	no	99.976	yes
[Ref.: #69480]	thermophile	no	99.919	no
[Ref.: #69480]	motile	no	81.436	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinobacillus seminis strain NCTC10851 PATRIC: 722.7
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #16243]

Culture collection no.

DSM 22263, ATCC 15768, CCUG 27187, CIP 102633, NCTC 10851, Simmons K3844-C

[Ref.: #20218]

Associated Passport(s) in StrainInfo

98655, 126621, 148179, 98657, 126622

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Sequence of Actinobacillus seminis Strain ATCC 15768, a Reference Strain of Ovine Pathogens That Causes Infections in Reproductive Organs.	Negrete-Abascal E, Montes-Garcia F, Vaca-Pacheco S, Leyto-Gil AM, Fragoso-Garcia E, Carvente-Garcia R, Perez-Agueros S, Castelan-Sanchez HG, Garcia-Molina A, Villamar TE, Sanchez-Alonso P, Vazquez-Cruz C	Genome Announc	10.1128/genomeA.01453-17	2018	*
Phylogeny	Isolation of Actinobacillus seminis from the genital tract of rams in spain.	de la Puente-Redondo VA, Garcia del Blanco N, Perez-Martinez C, Gonzalez-Rodriguez MC, Rodriguez-Ferri EF, Gutierrez-Martin CB	J Comp Pathol	10.1053/jcpa.1999.0359	2000	*
Enzymology	Cellular fatty acid and soluble protein composition of Actinobacillus actinomycetemcomitans and related organisms.	Calhoon DA, Mayberry WR, Slots J	J Clin Microbiol	10.1128/jcm.14.4.376-382.1981	1981	*

References

#16243

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22263

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40386 ; Curators of the CIP;
#48675 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 27187

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#68377

Automatically annotated from API NH .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Actinobacillus seminis strain NCTC10851

Actinobacillus seminis strain NCTC10851

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