Actinobacillus seminis (DSM 22263, ATCC 15768, CCUG 27187)

Name: Actinobacillus seminis (DSM 22263, ATCC 15768, CCUG 27187)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11683

Type strain

Strain Designation 5, A51 and A65, K3844/C

Culture col. no. DSM 22263 ATCC 15768 CCUG 27187 CIP 102633 NCTC 10851 4 more

NCBI tax ID(s) 722

Special Collections DSMZ CCUG CIP

Links

Actinobacillus seminis 5 is an obligate aerobe, mesophilic, Gram-negative animal pathogen that was isolated from sheep semen.

Gram-negative rod-shaped obligate aerobe mesophilic animal pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 36478

ATCC 15768,NCTC 10851,CCUG 27187,CIP 102633,DSM 22263

@ref 20215

Last LPSN update 2025-12-16 09:49:39

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pasteurellales

Family Pasteurellaceae

Genus Actinobacillus

Species Actinobacillus seminis

Full scientific name Actinobacillus seminis (ex Baynes and Simmons 1960) Sneath and Stevens 1990

BacDive ID	Other strains from **Actinobacillus seminis** (4)	Type strain
142528	A. seminis CCUG 12832
143362	A. seminis CCUG 18730
143363	A. seminis CCUG 18731
144191	A. seminis CCUG 23440

Morphology

Cell morphology

@ref	Gram stain	Cell shape
122554	negative	rod-shaped
125438
125438	negative

Colony morphology

16243

Type of hemolysisgamma

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16243	BHI MEDIUM (DSMZ Medium 215)	Medium recipe at MediaDive	Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
40386	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
122554	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16243	positive	growth	37	mesophilic
40386	positive	growth	37	mesophilic
122554	positive	growth	25-41
122554	negative	growth	15
122554	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
122554	obligate aerobe
125439	microaerophile

Spore formation

@ref	Spore formation	Confidence
125438		95.411
125439		94.4

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68377	17634 ChEBI	D-glucose	+	builds acid from	from API NH
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
122554	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
122554	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68377	17306 ChEBI	maltose	-	builds acid from	from API NH
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
122554	17632 ChEBI	nitrate	+	reduction
122554	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68377	27897 ChEBI	tryptophan	-	energy source	from API NH
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68377	16199 ChEBI	urea	-	hydrolysis	from API NH
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68377	35581 ChEBI	indole		from API NH
122554	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
122554	15688 ChEBI	acetoin	-
68377	35581 ChEBI	indole		from API NH

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
122554	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68377	alkaline phosphatase	-	3.1.3.1	from API NH
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
122554	beta-galactosidase	+	3.2.1.23
68377	beta-galactosidase	-	3.2.1.23	from API NH
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
16243	catalase	-	1.11.1.6
122554	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
16243	cytochrome-c oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
122554	gamma-glutamyltransferase	+	2.3.2.2
68382	leucine arylamidase	+	3.4.11.1	from API zym
68377	lipase	-		from API NH
68382	lipase (C 14)	-		from API zym
122554	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122554	ornithine decarboxylase	+	4.1.1.17
122554	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
122554	tryptophan deaminase	-
68377	tryptophan deaminase	-	4.1.99.1	from API NH
122554	urease	-	3.5.1.5
68377	urease	-	3.5.1.5	from API NH
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	L-lactaldehyde degradation	100	3 of 3
66794	biotin biosynthesis	100	4 of 4
66794	vitamin K metabolism	100	5 of 5
66794	coenzyme A metabolism	100	4 of 4
66794	ppGpp biosynthesis	100	4 of 4
66794	gluconeogenesis	100	8 of 8
66794	ubiquinone biosynthesis	100	7 of 7
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	palmitate biosynthesis	95.45	21 of 22
66794	chorismate metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	photosynthesis	85.71	12 of 14
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	pentose phosphate pathway	81.82	9 of 11
66794	threonine metabolism	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	starch degradation	80	8 of 10
66794	methylglyoxal degradation	80	4 of 5
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	pyrimidine metabolism	77.78	35 of 45
66794	vitamin B1 metabolism	76.92	10 of 13
66794	glycogen biosynthesis	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	purine metabolism	72.34	68 of 94
66794	phenylalanine metabolism	69.23	9 of 13
66794	degradation of sugar alcohols	68.75	11 of 16
66794	serine metabolism	66.67	6 of 9
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	formaldehyde oxidation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	methionine metabolism	65.38	17 of 26
66794	glycolysis	64.71	11 of 17
66794	glutathione metabolism	64.29	9 of 14
66794	proline metabolism	63.64	7 of 11
66794	urea cycle	61.54	8 of 13
66794	degradation of hexoses	61.11	11 of 18
66794	degradation of pentoses	60.71	17 of 28
66794	glutamate and glutamine metabolism	60.71	17 of 28
66794	glycogen metabolism	60	3 of 5
66794	Entner Doudoroff pathway	60	6 of 10
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	citric acid cycle	57.14	8 of 14
66794	heme metabolism	57.14	8 of 14
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	myo-inositol biosynthesis	50	5 of 10
66794	kanosamine biosynthesis II	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	NAD metabolism	50	9 of 18
66794	butanoate fermentation	50	2 of 4
66794	ketogluconate metabolism	50	4 of 8
66794	cysteine metabolism	50	9 of 18
66794	suberin monomers biosynthesis	50	1 of 2
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	histidine metabolism	48.28	14 of 29
66794	lysine metabolism	47.62	20 of 42
66794	tryptophan metabolism	47.37	18 of 38
66794	oxidative phosphorylation	47.25	43 of 91
66794	leucine metabolism	46.15	6 of 13
66794	alanine metabolism	44.83	13 of 29
66794	d-mannose degradation	44.44	4 of 9
66794	nitrate assimilation	44.44	4 of 9
66794	degradation of sugar acids	44	11 of 25
66794	cardiolipin biosynthesis	42.86	3 of 7
66794	tyrosine metabolism	42.86	6 of 14
66794	propanol degradation	42.86	3 of 7
66794	arginine metabolism	41.67	10 of 24
66794	hydrogen production	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	lipid metabolism	38.71	12 of 31
66794	vitamin B6 metabolism	36.36	4 of 11
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	cyanate degradation	33.33	1 of 3
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	enterobactin biosynthesis	33.33	1 of 3
66794	coenzyme M biosynthesis	30	3 of 10
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	ascorbate metabolism	27.27	6 of 22
66794	d-xylose degradation	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	sulfate reduction	23.08	3 of 13

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122554	-	+	+	+	-	+	-	-	-	-	+	+	-	-	+	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
122554	not determinedn.d.	+/-	-	-	+/-	+/-	+/-	-	-	-	-	-	+	-	-	-	-	+/-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	+/-

API NH

@ref	PEN	GLU	FRU	MAL	SAC	ODC	URE	LIP	PAL	beta GAL	ProA	GGT	IND
16243	+	+	-	-	+	-	-	-	-	-	+	-	-
16243	-	+	+	-	-	+	-	-	-	-	-	+	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
122554	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body Product	#Urogenital tract	#Semen

Isolation

@ref	Sample type
16243	sheep semen
48675	Sheep semen
122554	Animal, Sheep, semen

Taxonmaps

69479

Global distribution of 16S sequence AY362897 (>99% sequence identity) for Actinobacillus seminis subclade from Microbeatlas

Aquatic Samples	11
Soil Samples	5
Animal Samples	305
Plant Samples
Total Samples	321

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
16243		2	Risk group (German classification) According to TRBA 466
122554		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	49642_E01 assembly for Actinobacillus seminis NCTC10851	contig	GCA_900460625	722.7	2806310645	722	76.53
66792	ASM226321v1 assembly for Actinobacillus seminis ATCC 15768	contig	GCA_002263215	722.6	2834290966	722	71.36

Genome browser: GCA_900460625

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Actinobacillus seminis 16S ribosomal RNA gene, partial sequence, tRNA-Glu gene, complete sequence, and 23S ribosomal RNA gene, partial sequence	AF013275	562	722
20218	Actinobacillus seminis 16S ribosomal RNA gene, partial sequence, tRNA-Ile and tRNA-Ala genes, complete sequence, and 23S ribosomal RNA gene, partial sequence	AF013276	691	722
20218	Actinobacillus seminis strain ATCC 15768 16S ribosomal RNA gene, partial sequence	M75047	1479	722
16243	Actinobacillus seminis strain CCUG 27187 16S ribosomal RNA gene, partial sequence	AY362897	1364	722

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinobacillus seminis NCTC10851
NCBI: GCA_900460625

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.40	no
125439	motility	BacteriaNetⓘ	no	81.30	no
125439	gram_stain	BacteriaNetⓘ	positive	76.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	93.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinobacillus seminis strain ATCC 15768
PATRIC: 722.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.00	no
125438	anaerobic	anaerobicⓘ	no	95.50	yes
125438	spore-forming	spore-formingⓘ	no	95.41	no
125438	aerobic	aerobicⓘ	no	66.91	yes
125438	thermophilic	thermophileⓘ	no	96.00	yes
125438	flagellated	motile2+ⓘ	no	90.99	no

Literature

Title	Authors	Journal	DOI	Year
Identification of Actinobacillus seminis as the cause of abortion in sheep by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and whole genome sequencing.	Rondinone V, Palazzo L, Bianco A, La Rosa G, Manzulli V, Galante D, Del Sambro L, Toce M, Romano AC, Pace L.	Ital J Food Saf	10.4081/ijfs.2025.13384	2025
Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams.	Moustacas VS, Silva TM, Costa LF, Xavier MN, Carvalho CA, Costa EA, Paixao TA, Santos RL.	BMC Vet Res	10.1186/1746-6148-9-51	2013
Clinical and pathological changes in rams experimentally infected with Actinobacillus seminis and Histophilus somni.	Moustacas VS, Silva TM, Costa LF, Carvalho Junior CA, Santos RL, Paixao TA.	ScientificWorldJournal	10.1155/2014/241452	2014
Identification of an immunogenic protein of Actinobacillus seminis that is present in microvesicles.	Nunez-del Arco A, Salas-Tellez E, de la Garza M, Diaz-Aparicio E, Tenorio-Gutierrez V.	Can J Vet Res		2006
Adherence of actinobacillus pleuropneumoniae serotype 1 to swine buccal epithelial cells involves fibronectin.	Hamer-Barrera R, Godinez D, Enriquez VI, Vaca-Pacheco S, Martinez-Zuniga R, Talamas-Rohana P, Suarez-Guemez F, de la Garza M.	Can J Vet Res		2004
Genomic fingerprinting of "Haemophilus somnus" isolates by using a random-amplified polymorphic DNA assay.	Myers LE, Silva SV, Procunier JD, Little PB.	J Clin Microbiol	10.1128/jcm.31.3.512-517.1993	1993
Morphological, biochemical, antigenic, and cytochemical relationships among Haemophilus somnus, Haemophilus agni, Haemophilus haemoglobinophilus, Histophilus ovis, and Actinobacillus seminis.	Stephens LR, Humphrey JD, Little PB, Barnum DA.	J Clin Microbiol	10.1128/jcm.17.5.728-737.1983	1983
A model for demonstrating the adhesion of Actinobacillus seminis to epithelial cells.	Healey MC, Hwang HH, Elsner YY, Johnston AV.	Can J Vet Res		1991
An evaluation of the API ZYM system as a means of identifying Haemophilus somnus and related taxa.	Groom SC, Hazlett MJ, Little PB.	Can J Vet Res		1986
Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences.	Dewhirst FE, Paster BJ, Olsen I, Fraser GJ.	J Bacteriol	10.1128/jb.174.6.2002-2013.1992	1992
Enzymatic characterization of some oral and nonoral gram-negative bacteria with the API ZYM system.	Slots J.	J Clin Microbiol	10.1128/jcm.14.3.288-294.1981	1981
Compilation of small ribosomal subunit RNA structures.	Neefs JM, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R.	Nucleic Acids Res	10.1093/nar/21.13.3025	1993
PCR methods for rapid identification and characterization of Actinobacillus seminis strains.	Appuhamy S, Coote JG, Low JC, Parton R.	J Clin Microbiol	10.1128/jcm.36.3.814-817.1998	1998
Actinobacillus seminis DnaK interacts with bovine transferrin, lactoferrin, and hemoglobin as a putative iron acquisition mechanism.	Vazquez-Cruz C, Reyes-Malpica E, Montes-Garcia JF, Bautista-Betancourt P, Cobos-Justo E, Avalos-Rangel MA, Negrete-Abascal E.	Folia Microbiol (Praha)	10.1007/s12223-025-01271-7	2025
Testosterone and estradiol regulate the expression of proteases and a hemoglobinase in Actinobacillus seminis.	Ramirez-Paz-Y-Puente GA, Gutierrez-Pabello JA, Zenteno E, Villamar-Duque TE, Meneses-Romero EP, Vazquez-Cruz C, Negrete-Abascal E.	FEMS Microbiol Lett	10.1093/femsle/fnaf097	2025
Testosterone and estradiol modify the expression of adhesins and biofilm formation in Actinobacillus seminis.	Ramirez-Paz-Y-Puente GA, Chavez-Flores CI, Montes-Garcia JF, Sanchez-Alonso PG, Cobos-Justo ME, Vazquez-Cruz C, Zenteno E, Negrete-Abascal E.	FEMS Microbiol Lett	10.1093/femsle/fnad048	2023
Characterization of Actinobacillus seminis biofilm formation.	Garcia JFM, Rojas L, Zenteno E, Cruz CV, Abascal EN.	Antonie Van Leeuwenhoek	10.1007/s10482-020-01447-w	2020
Semen quality and pathological lesions in rams naturally infected with Brucella ovis during an outbreak in Saskatchewan.	Wickramasingha D, Schumann F, Sayi S, Toews A, Gabadage K, Fernandopulle R, Dadarwal D.	Can Vet J		2025
Actinobacillus seminis GroEL-homologous protein agglutinates sheep erythrocytes.	Montes-Garcia JF, Delgado-Tapia WA, Vazquez-Cruz C, Vaca S, Cruz-Cordova A, Negrete-Abascal E.	Antonie Van Leeuwenhoek	10.1007/s10482-019-01292-6	2019
Differences in Microbial Community Composition between Uterine Horns Ipsilateral and Contralateral to the Corpus Luteum in Beef Cows on Day 15 of the Estrous Cycle.	Walker MB, Holton MP, Callaway TR, Lourenco JM, Fontes PLP.	Microorganisms	10.3390/microorganisms11082117	2023
Identification of a novel beta-1,4-galactosyltransferase from Haemophilus parainfluenzae for efficient biosynthesis of lacto-N-neotetraose in Escherichia coli.	Liu D, Ying C, Ping Y, Liang S, Yan Q, Jiang Z.	Synth Syst Biotechnol	10.1016/j.synbio.2025.09.006	2026
Microbiological, molecular, and histopathological findings in goats experimentally infected with Actinobacillus seminis.	Santos FA, da Costa DF, da Silva AF, Pessoa RMDS, Figueiredo Rocha VC, Olinda RG, Dantas AFM, de Melo MA, Pena Alfaro CE, Azevedo SS, Alves CJ.	Microb Pathog	10.1016/j.micpath.2019.103555	2019
Urethral obstruction in a ram with a periurethral abscess: clinical findings, diagnostic imaging and pathology.	Pas ML, Chantillon L, Clinquart J, Hiltrop R, Vandekerckhove L, Pardon B, Govaere J, Meesters M.	BMC Vet Res	10.1186/s12917-024-04363-7	2024
Combined analysis of 16S rRNA gene sequencing data reveals core vaginal bacteria across livestock species.	Jonas LC, Youngs CR, Schmitz-Esser S.	Front Microbiol	10.3389/fmicb.2025.1524000	2025
Temporal changes in ewe vaginal microbiota throughout gestation.	Cassas MS, Jonas LC, Anderson CJ, Schmitz-Esser S, Youngs CR.	Front Microbiol	10.3389/fmicb.2024.1359678	2024
Description of the vaginal microbiota in nulliparous ewes during natural mating and pregnancy: preliminary signs of the male preputial microbiota modulation.	Barba M, Toquet M, Garcia-Rosello E, Gomis J, Quereda JJ, Gonzalez-Torres P, Carbonetto B, Gomez-Martin A.	Front Microbiol	10.3389/fmicb.2023.1224910	2023
Microbial diseases of the genital system of rams or bucks.	Gouletsou PG, Fthenakis GC.	Vet Microbiol	10.1016/j.vetmic.2015.07.016	2015
Characterizing the vaginal microbiota of high and low producing Poll Merino and White Suffolk ewes.	Greenwood EC, Torok VA, van Wettere WHEJ.	Transl Anim Sci	10.1093/tas/txac133	2022
Influence of the Ovine Genital Tract Microbiota on the Species Artificial Insemination Outcome. A Pilot Study in Commercial Sheep Farms.	Serrano M, Climent E, Freire F, Martinez-Blanch JF, Gonzalez C, Reyes L, Solaz-Fuster MC, Calvo JH, Jimenez MA, Codoner FM.	High Throughput	10.3390/ht9030016	2020
Emergence of a floR-carrying plasmid in extended spectrum beta-lactamase (ESBL) producing Pasteurella aerogenes, isolated from an avian species in China.	Yuan D, Wang M, Jia R, Chen S, Liu M, Zhao X, Yang Q, Wu Y, Zhang S, Huang J, Ou X, Mao S, Gao Q, Sun D, Tian B, Zhu D, Cheng A.	Poult Sci	10.1016/j.psj.2022.102207	2022
Isolation and Characterization of Thermophilic Bacteria from a Hot Spring in the State of Hidalgo, Mexico, and Geochemical Analysis of the Thermal Water	Ortega-Villar R, Escalante A, Astudillo-Melgar F, Lizarraga-Mendiola L, Vazquez-Rodriguez G, Hidalgo-Lara M, Coronel-Olivares C.	Microorganisms		2024
Is the IL1RA/IL1B Ratio a Suitable Biomarker for Subclinical Endometritis in Dairy Cows?	Kneidl AM, Marth CD, Kirsch S, Weber F, Zablotski Y, Helfrich AL, Schabmeyer ST, Schneider JK, Petzl W, Zerbe H, Meyerholz-Wohllebe MM.	Animals (Basel)	10.3390/ani12233363	2022
Isolation of Actinobacillus seminis from a goat with clinical epididymo-orchitis in Brazil.	dos Santos FA, de Azevedo EO, de Azevedo SS, Garino Junior F, Mota RA, de Cassia Peixoto Kim P, Gomes AL, Alves CJ.	Braz J Microbiol	10.1590/s1517-83822014005000024	2014
Effect of extender supplementation with various antimicrobial agents on viability of Brucella ovis and Actinobacillus seminis in cryopreserved ovine semen.	Moustacas VS, Xavier MN, Carvalho-Junior CA, Costa EA, Henry M, Santos RL.	Theriogenology	10.1016/j.theriogenology.2010.06.019	2010
Pathological changes in accessory sex organs of rams following experimental infection with Actinobacillus seminis.	Al-Katib W, Dennis S.	N Z Vet J	10.1080/00480169.2008.36853	2008
Diagnostic Findings in a Confirmed Outbreak of Brucella ovis Infection in a Traditional Sheep Farm in Sicily (South-Italy).	Galluzzo P, Migliore S, Cascio S, Barreca S, Alfano M, Tagliarini A, Candela A, Piraino C, Galuppo L, Condorelli L, Hussein HA, Tittarelli M, Chiarenza G.	Pathogens	10.3390/pathogens10111472	2021
Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome.	Reinoso-Pelaez EL, Saura M, Gonzalez-Recio O, Gonzalez C, Fernandez A, Peiro-Pastor R, Lopez-Garcia A, Saborio-Montero A, Calvo JH, Ramon M, Serrano M.	Front Microbiol	10.3389/fmicb.2023.1063807	2023
Early sequential findings in the genitalia of rams experimentally infected with Actinobacillus seminis.	Al-Katib WA, Dennis SM.	N Z Vet J	10.1080/00480169.2008.36807	2008
Epididymal and testicular lesions in rams following experimental infection with Actinobacillus seminis.	Al-Katib WA, Dennis SM.	N Z Vet J	10.1080/00480169.2007.36754	2007
Multiplex PCR for the detection of Brucella ovis, Actinobacillus seminis and Histophilus somni in ram semen.	Saunders VF, Reddacliff LA, Berg T, Hornitzky M.	Aust Vet J	10.1111/j.1751-0813.2006.00098.x	2007
Multi-omics analysis reveals substantial linkages between the oral-gut microbiomes and inflamm-aging molecules in elderly pigs.	Qiao C, He M, Wang S, Jiang X, Wang F, Li X, Tan S, Chao Z, Xin W, Gao S, Yuan J, Li Q, Xu Z, Zheng X, Zhao J, Liu G.	Front Microbiol	10.3389/fmicb.2023.1250891	2023
Experimental transmission of Actinobacillus seminis infection to rams.	Al-Katib WA, Dennis SM.	Vet Rec	10.1136/vr.157.5.143	2005
Concurrent and long-term associations between the endometrial microbiota and endometrial transcriptome in postpartum dairy cows.	Moore SG, Ericsson AC, Behura SK, Lamberson WR, Evans TJ, McCabe MS, Poock SE, Lucy MC.	BMC Genomics	10.1186/s12864-019-5797-8	2019
Vaginal bacterial community composition and concentrations of estradiol at the time of artificial insemination in Brangus heifers.	Messman RD, Contreras-Correa ZE, Paz HA, Perry G, Lemley CO.	J Anim Sci	10.1093/jas/skaa178	2020
Specific PCR primers from the 16S-23S rRNA spacer region for the rapid detection and identification of Actinobacillus seminis.	Appuhamy S, Low JC, Parton R, Coote JG.	J Appl Microbiol	10.1111/j.1365-2672.1998.tb05257.x	1998
Prevalence of Actinobacillus seminis in the semen of rams in the United Kingdom.	Low JC, Somerville D, Mylne MJ, McKelvey WA.	Vet Rec	10.1136/vr.136.11.268	1995
Apx toxins in Pasteurellaceae species from animals.	Schaller A, Kuhnert P, de la Puente-Redondo VA, Nicolet J, Frey J.	Vet Microbiol	10.1016/s0378-1135(00)00204-2	2000
Attempted definition by immunoblotting of the causes of reactivity in suspected false-positive sera in the Brucella ovis complement fixation test.	Kittelberger R, Laybourn BJ, Reichel MP, Ross GP, de Lisle GW, Joyce MA.	N Z Vet J	10.1080/00480169.1996.35966	1996
Isolation of Actinobacillus seminis from rams in the United Kingdom.	Heath PJ, Davies IH, Morgan JH, Aitken IA.	Vet Rec	10.1136/vr.129.14.304	1991
Genetic homogeneity of Actinobacillus seminis isolates.	McGillivery DJ, Webber JJ.	Res Vet Sci	10.1016/s0034-5288(18)31195-0	1989
Trials with an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of subclinical genital infections in rams caused by Histophilus ovis and Actinobacillus seminis.	Tekes L, Hajtos I.	Zentralbl Veterinarmed B	10.1111/j.1439-0450.1990.tb01095.x	1990
Comparative Review of Brucellosis in Small Domestic Ruminants.	Rossetti CA, Maurizio E, Rossi UA.	Front Vet Sci	10.3389/fvets.2022.887671	2022
Rapid identification of Haemophilus somnus, Histophilus ovis and Actinobacillus seminis using the API ZYM system.	Cousins DV, Lloyd JM.	Vet Microbiol	10.1016/0378-1135(88)90081-8	1988
Prevalence of Histophilus ovis and Actinobacillus seminis in the genital tract of sheep.	Walker RL, LeaMaster BR.	Am J Vet Res		1986
Pathology of acute experimental Actinobacillus seminis mastitis in ewes.	Alsenosy AM, Dennis SM.	Aust Vet J	10.1111/j.1751-0813.1985.tb07320.x	1985
Detection of antibody in rams with contagious epididymitis, using the enzyme-linked immunosorbent assay.	Cardenas AL, Maki LR.	Am J Vet Res		1986
Evaluation of three LPS-specific monoclonal antibodies for the immunodiagnosis of bovine brucellosis.	Yong WK, Edwards LD, Searson JE.	Res Vet Sci	10.1016/s0034-5288(18)31191-3	1989
The usefulness of the API 20 E classification system in the identification of Actinobacillus actinomycetem comitans, Actinobacillus seminis and Pasteurella haemolytica.	Erasmus JA.	Onderstepoort J Vet Res		1983
A study for the differentiation of Actinobacillus seminis, A. actinomycetem-comitans, Histophilus ovis and Pasteurella haemolytica.	Swanepoel ML.	Onderstepoort J Vet Res		1984
Use of monoclonal antibodies to identify outer membrane antigens of Actinobacillus species.	Healey MC, Gharpure HM, Kleinschuster SJ, Hwang HH, Johnston AV.	Am J Vet Res	10.2460/ajvr.1986.47.07.1446	1986
Ovine contagious epididymitis: a review.	Burgess GW.	Vet Microbiol	10.1016/0378-1135(82)90049-9	1982
Cultural and biochemical characterization of Actinobacillus and Actinobacillus-like species from ram lambs with epididymitis.	Scanlan CM, Healey MC, Torres AR, Johnston AV.	J Vet Diagn Invest	10.1177/104063878900100402	1989
Evaluating the reproductive ability of breeding rams in North-Eastern Spain using clinical examination of the body and external genitalia.	Mozo R, Galeote AI, Alabart JL, Fantova E, Folch J.	BMC Vet Res	10.1186/s12917-015-0600-9	2015
Assessment of the diagnostic sensitivity and specificity of an indirect ELISA kit for the diagnosis of Brucella ovis infection in rams.	Praud A, Champion JL, Corde Y, Drapeau A, Meyer L, Garin-Bastuji B.	BMC Vet Res	10.1186/1746-6148-8-68	2012
Association of age of ram with distribution of epididymal lesions and etiologic agent.	Walker RL, LeaMaster BR, Stellflug JN, Biberstein EL.	J Am Vet Med Assoc	10.2460/javma.1986.188.04.393	1986
Comparison and partial characterization of the protein profiles and outer membrane antigens of Actinobacillus species isolated from ram lambs with epididymitis.	Healey MC, Hwang HH, Kleinschuster SJ, Johnston AV, Symons KS.	Am J Vet Res	10.2460/ajvr.1988.49.11.1824	1988
Serological comparison between Histophilus ovis, Actinobacillus seminis and Brucella ovis.	Rahaley RS.	Aust Vet J	10.1111/j.1751-0813.1978.tb05567.x	1978
Characterisation of Histophilus ovis and related organisms by restriction endonuclease analysis.	McGillivery DJ, Webber JJ, Dean HF.	Aust Vet J	10.1111/j.1751-0813.1986.tb15880.x	1986
Epididymitis of rams in the central and southern districts of the Orange Free State.	De Wet JA, Erasmus JA.	J S Afr Vet Assoc		1984
Ram epididymitis: A clinical report.	Swift BL, Craddock F, Hancock HA, Jensen R, Thomas GM, Trueblood MS, Weibel J.	Theriogenology	10.1016/0093-691x(82)90094-2	1982
Bacteriological characteristics of Histophilus ovis and its relationship to similar bacteria.	Webb RF.	Res Vet Sci	10.1016/s0034-5288(18)32197-0	1983
Actinobacillus seminis infection in sheep in the Republic of South Africa. III. Growth and cultural characteristics of A. seminis.	van Tonder EM.	Onderstepoort J Vet Res		1979
Diagnosing ovine epididymitis by immunofluorescence.	Ajai CO, Cook JE, Dennis SM.	Vet Rec	10.1136/vr.107.18.421	1980
Testicular pathology of Merino rams.	Watt DA.	Aust Vet J	10.1111/j.1751-0813.1978.tb00291.x	1978
Genome Sequence of Actinobacillus seminis Strain ATCC 15768, a Reference Strain of Ovine Pathogens That Causes Infections in Reproductive Organs.	Negrete-Abascal E, Montes-Garcia F, Vaca-Pacheco S, Leyto-Gil AM, Fragoso-Garcia E, Carvente-Garcia R, Perez-Agueros S, Castelan-Sanchez HG, Garcia-Molina A, Villamar TE, Sanchez-Alonso P, Vazquez-Cruz C	Genome Announc	10.1128/genomeA.01453-17	2018
Isolation of Actinobacillus seminis from the genital tract of rams in spain.	de la Puente-Redondo VA, Garcia del Blanco N, Perez-Martinez C, Gonzalez-Rodriguez MC, Rodriguez-Ferri EF, Gutierrez-Martin CB	J Comp Pathol	10.1053/jcpa.1999.0359	2000
Cellular fatty acid and soluble protein composition of Actinobacillus actinomycetemcomitans and related organisms.	Calhoon DA, Mayberry WR, Slots J	J Clin Microbiol	10.1128/jcm.14.4.376-382.1981	1981
Actinobacillus rossii sp. nov., Actinobacillus seminis sp. nov., nom. rev., Pasteurella bettii sp. nov., Pasteurella lymphangitidis sp. nov., Pasteurella mairi sp. nov., and Pasteurella trehalosi sp. nov.	Sneath PH, Stevens M.	Int J Syst Bacteriol	10.1099/00207713-40-2-148	1990

References

#16243	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22263
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40386	; Curators of the CIP;
#48675	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 27187
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68371	Automatically annotated from API 50CH acid .
#68377	Automatically annotated from API NH .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122554	Collection of Institut Pasteur ; Curators of the CIP; CIP 102633
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinobacillus seminis (DSM 22263, ATCC 15768, CCUG 27187)

Name and taxonomic classification

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Physiology and metabolism

Oxygen tolerance

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Metabolite utilization

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API zym

API 50CHac

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API biotype100

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Genome browser: GCA_900460625

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