Strain identifier

BacDive ID: 11684

Type strain: Yes

Species: Actinobacillus porcinus

Strain Designation: NM319, NM 319

Strain history: CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain NM 319 <- K. Moeller <- S. Rosendal

NCBI tax ID(s): 51048 (species)

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General

@ref: 16331

BacDive-ID: 11684

DSM-Number: 22435

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Actinobacillus porcinus NM319 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from pig upper respiratory tract.

NCBI tax id

  • NCBI tax id: 51048
  • Matching level: species

strain history

@refhistory
16331<- CCUG; NM319 <- K. Möller, Danish Vet. Lab., Copenhagen, Denmark <- S. Rosendal, Univ. Guelph, Ontario, Canada
121944CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain NM 319 <- K. Moeller <- S. Rosendal

doi: 10.13145/bacdive11684.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus porcinus
  • full scientific name: Actinobacillus porcinus Møller et al. 1996

@ref: 16331

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus porcinus

full scientific name: Actinobacillus porcinus Møller et al. 1996

strain designation: NM319, NM 319

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.947
121944negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16331HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://mediadive.dsmz.de/medium/804Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
39259MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
121944CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
16331positivegrowth37mesophilic
39259positivegrowth37mesophilic
54557positivegrowth37mesophilic
121944positivegrowth25-41
121944nogrowth15psychrophilic
121944nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16331microaerophile
16331aerobe
54557microaerophile
121944facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.984

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
121944esculin-hydrolysis4853
121944hippurate-hydrolysis606565
121944nitrate+reduction17632
121944nitrite-reduction16301
68377D-glucose+builds acid from17634
68377D-fructose+builds acid from15824
68377maltose+builds acid from17306
68377ornithine-degradation18257
68377urea-hydrolysis16199
68377tryptophan-energy source27897

antibiotic resistance

  • @ref: 121944
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
12194435581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
16331catalase+1.11.1.6
16331cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
121944oxidase-
121944beta-galactosidase+3.2.1.23
121944alcohol dehydrogenase-1.1.1.1
121944catalase-1.11.1.6
121944gamma-glutamyltransferase-2.3.2.2
121944lysine decarboxylase-4.1.1.18
121944ornithine decarboxylase-4.1.1.17
121944urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121944-++-------+-++-----+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121944+---++----+++----++--+/-+/-+++/-+/-+++++--+-+/----------+-+

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16331++++----+-+--
16331-++++----+-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16331pig upper respiratory tractOntarioCanadaCANNorth America
54557Pig upper respiratory tractGuelphCanadaCANNorth America
121944Animal, Pig, respiratory tractOntarioCanadaCANNorth America

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Oral cavity and airways#Airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_158891.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_14831;97_18110;98_22526;99_158891&stattab=map
  • Last taxonomy: Actinobacillus porcinus subclade
  • 16S sequence: AY362896
  • Sequence Identity:
  • Total samples: 779
  • soil counts: 2
  • aquatic counts: 5
  • animal counts: 768
  • plant counts: 4

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
163311Risk group (German classification)
1219441Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16331
  • description: Actinobacillus porcinus strain CCUG 38924 16S ribosomal RNA gene, partial sequence
  • accession: AY362896
  • length: 1364
  • database: ena
  • NCBI tax ID: 51048

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinobacillus porcinus NM319GCA_901764995scaffoldncbi51048
66792Actinobacillus porcinus NM319GCA_900773985scaffoldncbi51048

GC content

  • @ref: 16331
  • GC-content: 41.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno95.457no
gram-positiveno98.536no
anaerobicno93.808yes
aerobicno94.282no
halophileno56.177no
spore-formingno98.73no
thermophileno97.891no
glucose-utilyes57.941no
motileno90.248no
glucose-fermentyes56.526no

External links

@ref: 16331

culture collection no.: DSM 22435, CCUG 38924, CIP 105315

straininfo link

  • @ref: 80904
  • straininfo: 51123

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8863422Actinobacillus minor sp. nov., Actinobacillus porcinus sp. nov., and Actinobacillus indolicus sp. nov., three new V factor-dependent species from the respiratory tract of pigs.Moller K, Fussing V, Grimont PA, Paster BJ, Dewhirst FE, Kilian MInt J Syst Bacteriol10.1099/00207713-46-4-9511996Actinobacillus/*classification, Animals, Base Sequence, DNA, Bacterial/analysis, DNA, Ribosomal/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Respiratory System/*microbiology, Swine/*microbiologyGenetics
Genetics31896621Draft Genome Sequences of the Type Strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), Two NAD-Dependent Bacterial Species Found in the Respiratory Tract of Pigs.Bosse JT, Li Y, Fernandez Crespo R, Angen O, Holden MTG, Weinert LA, Maskell DJ, Tucker AW, Wren BW, Rycroft AN, Langford PRMicrobiol Resour Announc10.1128/MRA.00716-192020

Reference

@idauthorscataloguedoi/urltitle
16331Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22435)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22435
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39259Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17321
54557Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 38924)https://www.ccug.se/strain?id=38924
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80904Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID51123.1StrainInfo: A central database for resolving microbial strain identifiers
121944Curators of the CIPCollection of Institut Pasteur (CIP 105315)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105315