Name: Thermobacillus composti KWC4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11666

Type strain:

Species: Thermobacillus composti

Strain Designation: KWC4

Culture col. no.: DSM 18247, JCM 13945

Strain history: N. Kurosawa KWC4.

NCBI tax ID(s): 717605 (strain), 377615 (species)

SI-ID 309511

DSM 18247, JCM 13945

Section

Thermobacillus composti KWC4 is an aerobe, spore-forming, thermophilic bacterium that was isolated from fed-batch composting reactor.

Gram-negative
rod-shaped
spore-forming
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Thermobacillus*
	Species	**Thermobacillus composti**
	Full Scientific Name (LPSN)	Thermobacillus composti Watanabe et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31933]	Gram stain	negative

[Ref.: #31933]	Cell length	3.5 µm

[Ref.: #31933]	Cell width	0.6 µm

[Ref.: #31933]	Cell shape	rod-shaped

[Ref.: #31933]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7456]

Culture medium

SULFOLOBUS MEDIUM (DSMZ Medium 88)

[Ref.: #7456]

Culture medium growth

[Ref.: #7456]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7456]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7456]	growth	50 °C
[Ref.: #31933]	growth	32-61 °C
[Ref.: #31933]	optimum	50 °C
[Ref.: #67770]	growth	50 °C

[Ref.: #7456]	Temperature range	thermophilic
[Ref.: #31933]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #31933]			optimum	9
[Ref.: #31933]			growth	5.6-10.1

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31933]

Oxygen tolerance

aerobe

[Ref.: #31933]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31933]		NaCl		growth	0.0-4.4	%

[Ref.: #31933]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-6(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31933]	cellobiose ChEBI	+	carbon source
[Ref.: #31933]	dextrin ChEBI	+	carbon source
[Ref.: #31933]	fructose ChEBI	+	carbon source
[Ref.: #31933]	galactose ChEBI	+	carbon source
[Ref.: #31933]	glucose ChEBI	+	carbon source
[Ref.: #31933]	lactose ChEBI	+	carbon source
[Ref.: #31933]	maltose ChEBI	+	carbon source
[Ref.: #31933]	mannose ChEBI	+	carbon source
[Ref.: #31933]	melibiose ChEBI	+	carbon source
[Ref.: #31933]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #31933]	sucrose ChEBI	+	carbon source
[Ref.: #31933]	trehalose ChEBI	+	carbon source
[Ref.: #31933]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #31933]	acid phosphatase	+	3.1.3.2
[Ref.: #31933]	alkaline phosphatase	+	3.1.3.1
[Ref.: #31933]	alpha-galactosidase	+	3.2.1.22
[Ref.: #31933]	catalase	+	1.11.1.6
[Ref.: #31933]	cytochrome oxidase	+	1.9.3.1

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	starch degradation	90	9 of 10
[Ref.: #66794]	Entner Doudoroff pathway	90	9 of 10
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	degradation of sugar alcohols	87.5	14 of 16
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	vitamin B1 metabolism	84.62	11 of 13
[Ref.: #66794]	methionine metabolism	84.62	22 of 26
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
[Ref.: #66794]	pentose phosphate pathway	81.82	9 of 11
[Ref.: #66794]	flavin biosynthesis	80	12 of 15
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	gluconeogenesis	75	6 of 8
[Ref.: #66794]	purine metabolism	74.47	70 of 94
[Ref.: #66794]	d-xylose degradation	72.73	8 of 11
[Ref.: #66794]	tetrahydrofolate metabolism	71.43	10 of 14
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	pyrimidine metabolism	71.11	32 of 45
[Ref.: #66794]	oxidative phosphorylation	70.33	64 of 91
[Ref.: #66794]	urea cycle	69.23	9 of 13
[Ref.: #66794]	phenylalanine metabolism	69.23	9 of 13
[Ref.: #66794]	leucine metabolism	69.23	9 of 13
[Ref.: #66794]	non-pathway related	68.42	26 of 38
[Ref.: #66794]	degradation of pentoses	67.86	19 of 28
[Ref.: #66794]	degradation of hexoses	66.67	12 of 18
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	IAA biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	citric acid cycle	64.29	9 of 14
[Ref.: #66794]	degradation of sugar acids	64	16 of 25
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	cysteine metabolism	61.11	11 of 18
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	coenzyme M biosynthesis	60	6 of 10
[Ref.: #66794]	gallate degradation	60	3 of 5
[Ref.: #66794]	alanine metabolism	58.62	17 of 29
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	glutathione metabolism	57.14	8 of 14
[Ref.: #66794]	sulfate reduction	53.85	7 of 13
[Ref.: #66794]	tryptophan metabolism	52.63	20 of 38
[Ref.: #66794]	histidine metabolism	51.72	15 of 29
[Ref.: #66794]	lipid metabolism	51.61	16 of 31
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	4-hydroxyphenylacetate degradation	50	5 of 10
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	vitamin B6 metabolism	45.45	5 of 11
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	lysine metabolism	45.24	19 of 42
[Ref.: #66794]	4-hydroxymandelate degradation	44.44	4 of 9
[Ref.: #66794]	propionate fermentation	40	4 of 10
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	phenylpropanoid biosynthesis	38.46	5 of 13
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	38.46	5 of 13
[Ref.: #66794]	androgen and estrogen metabolism	37.5	6 of 16
[Ref.: #66794]	ascorbate metabolism	36.36	8 of 22
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	polyamine pathway	30.43	7 of 23
[Ref.: #66794]	phenol degradation	30	6 of 20
[Ref.: #66794]	tyrosine metabolism	28.57	4 of 14
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	3-phenylpropionate degradation	26.67	4 of 15
[Ref.: #66794]	vitamin B12 metabolism	26.47	9 of 34
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7456]	Sample type/isolated from	fed-batch composting reactor
[Ref.: #7456]	Geographic location (country and/or sea, region)	Hachioji, Tokyo
[Ref.: #7456]	Country	Japan
[Ref.: #7456]	Country ISO 3 Code	JPN
[Ref.: #7456]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Composting reactor
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Engineered	#Bioreactor	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB254031 (>99% sequence identity) for Thermobacillus from Microbeatlas

Aquatic Samples	1207
Soil Samples	4904
Animal Samples	1953
Plant Samples	1954
Total Samples	10018

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7456]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7456]	Thermobacillus composti gene for 16S ribosomal RNA, partial sequence	AB254031	1528	ENA	717605 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermobacillus composti KWC4	GCA_000227705	complete	GenBank	717605 tax ID	*
[Ref.: #66792]	Thermobacillus composti KWC4	717605.9	plasmid	PATRIC	717605 tax ID	*
[Ref.: #66792]	Thermobacillus composti KWC4, DSM 18247	2508501069	complete	JGI IMG/M	717605 tax ID	*

[Ref.: #7456]	GC-content	60 mol%
[Ref.: #67770]	GC-content	60 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermobacillus composti KWC4 KWC4 NCBI: GCA_000227705.3
[Ref.: #69480]	motile	yes	84.212	yes
[Ref.: #69480]	flagellated	yes	78.115	no
[Ref.: #69480]	gram-positive	yes	77.23	yes
[Ref.: #69480]	anaerobic	no	97.975	no
[Ref.: #69480]	aerobic	yes	86.059	yes
[Ref.: #69480]	halophile	no	94.516	no
[Ref.: #69480]	spore-forming	yes	95.773	no
[Ref.: #69480]	glucose-util	yes	87.71	no
[Ref.: #69480]	thermophile	no	57.854	no
[Ref.: #69480]	glucose-ferment	no	86.221	no

Availability in culture collections External links

[Ref.: #7456]

Culture collection no.

DSM 18247, JCM 13945

[Ref.: #80887]

SI-ID 309511

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermobacillus composti sp. nov., a moderately thermophilic bacterium isolated from a composting reactor.	Watanabe K, Nagao N, Yamamoto S, Toda T, Kurosawa N	Int J Syst Evol Microbiol	10.1099/ijs.0.64672-0	2007	*

References

#7456

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18247

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31933

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28190 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#80887

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID309511.1 )

#28190

IJSEM 1473 2007 ( DOI 10.1099/ijs.0.64672-0 , PubMed 17625178 )

* These data were automatically processed and therefore are not curated

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Thermobacillus composti KWC4 KWC4

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