Name: Brevibacillus thermoruber BT2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 11409

Type strain:

Species: Brevibacillus thermoruber

Strain Designation: BT2

Culture col. no.: DSM 7064, MIM 30.8.38, CCUG 54929, CIP 105255, CIP 105298, HSCC 624

Strain history: CIP <- 1997, O. Shida, Res. Lab., Chiba, Japan: strain HSCC 624 <- DSMZ <- P.L. Manachini

NCBI tax ID(s): 33942 (species)

SI-ID 7876

LMG 16910, DSM 7064, CIP 105255, KCTC 3575, HAMBI 2105, CIP 105298

Special Collections

Other strains from Brevibacillus thermoruber

Section

Brevibacillus thermoruber BT2 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from compost for production of mushrooms.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Brevibacillus*
	Species	**Brevibacillus thermoruber**
	Full Scientific Name (LPSN)	Brevibacillus thermoruber (Manachini et al. 1985 ex Guicciardi et al. 1968) Shida et al. 1996

Synonym

Bacillus thermoruber

Information on morphological and physiological properties Morphology

[Ref.: #123513]	Gram stain	positive

[Ref.: #123513]	Cell shape	rod-shaped

[Ref.: #123513]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2967]

Culture medium

GYE - MEDIUM (DSMZ Medium 572)

[Ref.: #2967]

Culture medium growth

[Ref.: #2967]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2967]

Culture medium composition

expand / minimize

[Ref.: #39239]

Culture medium

MEDIUM 220 - for Brevibacillus thermoruber

[Ref.: #39239]

Culture medium growth

[Ref.: #39239]

Culture medium composition

expand / minimize

[Ref.: #123513]

Culture medium

CIP Medium 220

[Ref.: #123513]

Culture medium growth

[Ref.: #123513]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123513]

Culture medium

CIP Medium 233

[Ref.: #123513]

Culture medium growth

[Ref.: #123513]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2967]		growth	45 °C
[Ref.: #39239]		growth	45 °C
[Ref.: #60390]		growth	42 °C
[Ref.: #123513]		growth	30-45 °C
[Ref.: #123513]	no	growth	10 °C
[Ref.: #123513]	no	growth	22 °C

	pH	Kind of pH		pH
[Ref.: #123513]		no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #60390]

Oxygen tolerance

aerobe

[Ref.: #123513]

Ability of spore formation

yes

	Salt	Tested relation		Salt conc.
[Ref.: #123513]	NaCl		growth	0	%
[Ref.: #123513]	NaCl	no	growth	2	%
[Ref.: #123513]	NaCl	no	growth	4	%
[Ref.: #123513]	NaCl	no	growth	6	%
[Ref.: #123513]	NaCl	no	growth	8	%
[Ref.: #123513]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123513]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123513]	nitrate ChEBI	-	reduction
[Ref.: #123513]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123513]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123513]		acetoin ChEBI	-
[Ref.: #123513]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123513]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123513]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123513]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123513]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #123513]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123513]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123513]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123513]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123513]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #123513]	10002	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123513]	8560	-	+	+	+	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2967]	Sample type/isolated from	compost for production of mushrooms

[Ref.: #60390]	Sample type/isolated from	Compost for the production of mushrooms

[Ref.: #123513]	Sample type/isolated from	Environment, Compost for production of mushrooms
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Engineered	#Food production	#Food
#Host	#Fungi	#Mushroom

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Z26921 (>99% sequence identity) for Brevibacillus thermoruber from Microbeatlas

Aquatic Samples	1512
Soil Samples	5730
Animal Samples	1992
Plant Samples	1394
Total Samples	10628

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2967]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123513]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Brevibacillus thermoruber gene for 16S rRNA, partial sequence, strain:DSM 7064T	AB112722	1500	GenBank ENA	33942 tax ID	*
[Ref.: #20218]	B.thermoruber gene for 16S ribosomal RNA	Z26921	1514	GenBank ENA	33942 tax ID	*

[Ref.: #2967]

GC-content

57.0 mol%

Availability in culture collections External links

[Ref.: #2967]

Culture collection no.

DSM 7064, MIM 30.8.38, CCUG 54929, CIP 105255, CIP 105298, HSCC 624

[Ref.: #80641]

SI-ID 7876

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevibacillus sediminis sp. nov., isolated from a hot spring.	Xian WD, Yin YR, Liu L, Yuan CG, Hussain F, Khan I, Habib N, Zhou EM, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000752	2015	*
Phylogeny	Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring.	Inan K, Canakci S, Belduz AO, Sahin F	Int J Syst Evol Microbiol	10.1099/ijs.0.031914-0	2011	*
Cultivation	Swarming behavior of a novel strain of Brevibacillus thermoruber.	Sakka K, Kihira M, Kuhara W, Mochida A, Kimura T, Sakka M	J Basic Microbiol	10.1002/jobm.202200445	2022	*

References

#2967

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7064

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39239 ; Curators of the CIP;
#60390 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54929

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80641

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7876.1 )

#123513 Collection of Institut Pasteur ; Curators of the CIP; CIP 105255
* These data were automatically processed and therefore are not curated

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