Brevibacillus thermoruber (DSM 7064, BT2, MIM 30.8.38)

Name: Brevibacillus thermoruber (DSM 7064, BT2, MIM 30.8.38)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11409

Type strain

Strain Designation BT2

Culture col. no. DSM 7064 BT2 MIM 30.8.38 CCUG 54929 CIP 105255 2 more

NCBI tax ID(s) 33942

Special Collections DSMZ CCUG CIP

Links

Brevibacillus thermoruber BT2 is an aerobe, spore-forming, thermophilic prokaryote that was isolated from compost for production of mushrooms.

spore-forming Gram-positive motile rod-shaped aerobe thermophilic 16S sequence

Name and taxonomic classification

SI-ID 7876

LMG 16910,DSM 7064,CIP 105255,KCTC 3575,HAMBI 2105,CIP 105298

@ref 20215

Last LPSN update 2025-12-16 09:49:35

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Paenibacillaceae

Genus Brevibacillus

Species Brevibacillus thermoruber

Full scientific name Brevibacillus thermoruber (Manachini et al. 1985 ex Guicciardi et al. 1968) Shida et al. 1996

Synonyms (1)

Bacillus thermoruber

BacDive ID	Other strains from **Brevibacillus thermoruber** (4)	Type strain
146824	B. thermoruber CCUG 32182
147240	B. thermoruber CCUG 33165
149782	B. thermoruber CCUG 38388
160340	B. thermoruber PM1, DSM 26550

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123513	positive	rod-shaped

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2967	GYE - MEDIUM (DSMZ Medium 572)	Medium recipe at MediaDive	Name: GYE - MEDIUM (DSMZ Medium 572) Composition: Glucose 15.0 g/l Yeast extract 5.0 g/l CaCl2 x 2 H2O 0.2 g/l Tap water
39239	MEDIUM 220 - for Brevibacillus thermoruber		Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (5.000g);Peptone (3.000 g);Tryptone (5.000 g);Sucrose (5.000 g)
123513	CIP Medium 220	Medium recipe at CIP
123513	CIP Medium 233	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
2967	positive	growth	45	thermophilic
39239	positive	growth	45	thermophilic
60390	positive	growth	42	thermophilic
123513	positive	growth	30-45
123513	negative	growth	10
123513	negative	growth	22

Culture pH

@ref	Ability	Type	PH
123513		growth	6

Physiology and metabolism

Oxygen tolerance

60390

Oxygen toleranceaerobe

Spore formation

123513

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123513	NaCl	positive	growth	0 %
123513	NaCl		growth	2 %
123513	NaCl		growth	4 %
123513	NaCl		growth	6 %
123513	NaCl		growth	8 %
123513	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
123513	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
123513	17632 ChEBI	nitrate	-	reduction
123513	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123513	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123513	15688 ChEBI	acetoin	-
123513	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123513	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
123513	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123513	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123513	gamma-glutamyltransferase	+	2.3.2.2
123513	gelatinase	+
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123513	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123513	ornithine decarboxylase	-	4.1.1.17
123513	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
123513	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123513	-	+	+	+	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123513	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Composting
#Engineered	#Food production	#Food
#Host	#Fungi	#Mushroom

Isolation

@ref	Sample type
2967	compost for production of mushrooms
60390	Compost for the production of mushrooms
123513	Environment, Compost for production of mushrooms

Taxonmaps

69479

Global distribution of 16S sequence Z26921 (>99% sequence identity) for Brevibacillus thermoruber from Microbeatlas

Aquatic Samples	1512
Soil Samples	5730
Animal Samples	1992
Plant Samples	1394
Total Samples	10628

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2967	1	Risk group (German classification) According to TRBA 466
123513	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Brevibacillus thermoruber gene for 16S rRNA, partial sequence, strain:DSM 7064T	AB112722	1500		33942
20218	B.thermoruber gene for 16S ribosomal RNA	Z26921	1514		33942

GC content

2967

GC-content (mol%)57.0

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria.	Benammar L, Inan Bektas K, Menasria T, Belduz AO, Guler HI, Bedaida IK, Gonzalez JM, Ayachi A.	Braz J Microbiol	10.1007/s42770-020-00376-0	2020
Metabolism	Genetic diversity of hydrogen-producing bacteria in an acidophilic ethanol-H2-coproducing system, analyzed using the [Fe]-hydrogenase gene.	Xing D, Ren N, Rittmann BE.	Appl Environ Microbiol	10.1128/aem.01946-07	2008
Cultivation	Swarming behavior of a novel strain of Brevibacillus thermoruber.	Sakka K, Kihira M, Kuhara W, Mochida A, Kimura T, Sakka M	J Basic Microbiol	10.1002/jobm.202200445	2022
Genetics	Rubeoparvulum massiliense gen. nov., sp. nov., a new bacterial genus isolated from the human gut of a Senegalese infant with severe acute malnutrition.	Tidjani Alou M, Rathored J, Lagier JC, Khelaifia S, Michelle C, Sokhna C, Diallo A, Diallo AB, Fournier PE, Raoult D, Edouard S.	New Microbes New Infect	10.1016/j.nmni.2016.11.003	2017
Phylogeny	Brevibacillus sediminis sp. nov., isolated from a hot spring.	Xian WD, Yin YR, Liu L, Yuan CG, Hussain F, Khan I, Habib N, Zhou EM, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000752	2015
Phylogeny	Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring.	Inan K, Canakci S, Belduz AO, Sahin F	Int J Syst Evol Microbiol	10.1099/ijs.0.031914-0	2011

References

#2967	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7064
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#39239	; Curators of the CIP;
#60390	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54929
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123513	Collection of Institut Pasteur ; Curators of the CIP; CIP 105255
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brevibacillus thermoruber (DSM 7064, BT2, MIM 30.8.38)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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