Strain identifier

BacDive ID: 11409

Type strain: Yes

Species: Brevibacillus thermoruber

Strain Designation: BT2

Strain history: CIP <- 1997, O. Shida, Res. Lab., Chiba, Japan: strain HSCC 624 <- DSMZ <- P.L. Manachini

NCBI tax ID(s): 33942 (species)

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General

@ref: 2967

BacDive-ID: 11409

DSM-Number: 7064

keywords: 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped

description: Brevibacillus thermoruber BT2 is an aerobe, spore-forming, thermophilic bacterium that was isolated from compost for production of mushrooms.

NCBI tax id

  • NCBI tax id: 33942
  • Matching level: species

strain history

@refhistory
2967<- P.L. Manachini
123513CIP <- 1997, O. Shida, Res. Lab., Chiba, Japan: strain HSCC 624 <- DSMZ <- P.L. Manachini

doi: 10.13145/bacdive11409.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Brevibacillus
  • species: Brevibacillus thermoruber
  • full scientific name: Brevibacillus thermoruber (Manachini et al. 1985 ex Guicciardi et al. 1968) Shida et al. 1996

  • synonyms

    • @ref: 20215
    • synonym: Bacillus thermoruber

@ref: 2967

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Brevibacillus

species: Brevibacillus thermoruber

full scientific name: Brevibacillus thermoruber (Manachini et al. 1985) Shida et al. 1996

strain designation: BT2

type strain: yes

Morphology

cell morphology

  • @ref: 123513
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 123513

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2967GYE - MEDIUM (DSMZ Medium 572)yeshttps://mediadive.dsmz.de/medium/572Name: GYE - MEDIUM (DSMZ Medium 572) Composition: Glucose 15.0 g/l Yeast extract 5.0 g/l CaCl2 x 2 H2O 0.2 g/l Tap water
39239MEDIUM 220 - for Brevibacillus thermoruberyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (5.000g);Peptone (3.000 g);Tryptone (5.000 g);Sucrose (5.000 g)
123513CIP Medium 220yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=220
123513CIP Medium 233yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=233

culture temp

@refgrowthtypetemperaturerange
2967positivegrowth45thermophilic
39239positivegrowth45thermophilic
60390positivegrowth42thermophilic
123513positivegrowth30-45
123513nogrowth10psychrophilic
123513nogrowth22psychrophilic

culture pH

  • @ref: 123513
  • ability: no
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 60390
  • oxygen tolerance: aerobe

spore formation

  • @ref: 123513
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
123513NaClpositivegrowth0 %
123513NaClnogrowth2 %
123513NaClnogrowth4 %
123513NaClnogrowth6 %
123513NaClnogrowth8 %
123513NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123513esculin-hydrolysis4853
123513nitrate-reduction17632
123513nitrite-reduction16301

metabolite production

  • @ref: 123513
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12351315688acetoin-
12351317234glucose-

enzymes

@refvalueactivityec
123513oxidase-
123513beta-galactosidase-3.2.1.23
123513alcohol dehydrogenase-1.1.1.1
123513gelatinase+
123513catalase+1.11.1.6
123513gamma-glutamyltransferase+2.3.2.2
123513lysine decarboxylase-4.1.1.18
123513ornithine decarboxylase-4.1.1.17
123513urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123513-+++--+---++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123513----+/-----------------------------------+/-+/-+/-------+/-

Isolation, sampling and environmental information

isolation

@refsample type
2967compost for production of mushrooms
60390Compost for the production of mushrooms
123513Environment, Compost for production of mushrooms

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Composting
#Engineered#Food production#Food
#Host#Fungi#Mushroom

taxonmaps

  • @ref: 69479
  • File name: preview.99_761.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_95;96_460;97_527;98_607;99_761&stattab=map
  • Last taxonomy: Brevibacillus thermoruber
  • 16S sequence: Z26921
  • Sequence Identity:
  • Total samples: 10628
  • soil counts: 5730
  • aquatic counts: 1512
  • animal counts: 1992
  • plant counts: 1394

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
29671Risk group (German classification)
1235131Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Brevibacillus thermoruber gene for 16S rRNA, partial sequence, strain:DSM 7064TAB1127221500ena33942
20218B.thermoruber gene for 16S ribosomal RNAZ269211514ena33942

GC content

  • @ref: 2967
  • GC-content: 57.0

External links

@ref: 2967

culture collection no.: DSM 7064, MIM 30.8.38, CCUG 54929, CIP 105255, CIP 105298, HSCC 624

straininfo link

  • @ref: 80641
  • straininfo: 7876

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21622837Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring.Inan K, Canakci S, Belduz AO, Sahin FInt J Syst Evol Microbiol10.1099/ijs.0.031914-02011Bacterial Typing Techniques, Base Composition, Brevibacillus/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hot Springs/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Turkey, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny26556809Brevibacillus sediminis sp. nov., isolated from a hot spring.Xian WD, Yin YR, Liu L, Yuan CG, Hussain F, Khan I, Habib N, Zhou EM, Li WJInt J Syst Evol Microbiol10.1099/ijsem.0.0007522015
Cultivation36190013Swarming behavior of a novel strain of Brevibacillus thermoruber.Sakka K, Kihira M, Kuhara W, Mochida A, Kimura T, Sakka MJ Basic Microbiol10.1002/jobm.2022004452022Agar, *Brevibacillus/genetics, Culture Media, DNA

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2967Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7064)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7064
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39239Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17254
60390Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 54929)https://www.ccug.se/strain?id=54929
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80641Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7876.1StrainInfo: A central database for resolving microbial strain identifiers
123513Curators of the CIPCollection of Institut Pasteur (CIP 105255)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105255