Rhodococcus ruber Chol-4 is a bacterium that was isolated from sewage sludge from sewage treatment plant of Ciudad Real.
genome sequence 16S sequence Bacteria| @ref 20215 |
Last LPSN update
2026-05-29 08:27:27 |
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Rhodococcus |
| Species Rhodococcus ruber |
| Full scientific name Rhodococcus ruber (Kruse 1896) Goodfellow and Alderson 1977 (Approved Lists 1980) |
| Synonyms (2) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 16153 | GPHF-MEDIUM (DSMZ Medium 553) | Medium recipe at MediaDive  | Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water | ||
| 16153 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | |
|---|---|---|---|---|
| 16153 | positive | growth | 28 |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Engineered | #Waste | #Sewage sludge | |
| #Engineered | #Waste | #Water treatment plant |
| @ref | Sample type | Geographic location | Country | Country ISO 3 Code | Continent | |
|---|---|---|---|---|---|---|
| 16153 | sewage sludge from sewage treatment plant of Ciudad Real | Ciudad Real | Spain | ESP | Europe |
Global distribution of 16S sequence EU878550 (>99% sequence identity) for Rhodococcus from Microbeatlas 
 Aquatic Samples |
725 |
 Soil Samples |
1219 |
 Animal Samples |
950 |
 Plant Samples |
247 |
| Total Samples | 3141 |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM34795v2 assembly for Rhodococcus ruber Chol-4 | scaffold | GCA_000347955   | 1240349.3  | 2554235023  | 1240349 | 69.26 |  |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 16153 | Rhodococcus ruber strain Chol-4 16S ribosomal RNA gene, partial sequence | EU878550 | 1348 |  | 1240349 |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Microbial degradation of steroid sex hormones: implications for environmental and ecological studies. | Chiang YR, Wei ST, Wang PH, Wu PH, Yu CP. | Microb Biotechnol | 10.1111/1751-7915.13504 | 2020 | | |
| Metabolism | Further Studies on the 3-Ketosteroid 9alpha-Hydroxylase of Rhodococcus ruber Chol-4, a Rieske Oxygenase of the Steroid Degradation Pathway. | Baldanta S, Navarro Llorens JM, Guevara G | Microorganisms | 10.3390/microorganisms9061171 | 2021 | |
| Metabolism | Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production. | Guevara G, Olortegui Flores Y, Fernandez de Las Heras L, Perera J, Navarro Llorens JM | PLoS One | 10.1371/journal.pone.0220492 | 2019 | |
| Genetics | New insights into the genome of Rhodococcus ruber strain Chol-4. | Guevara G, Castillo Lopez M, Alonso S, Perera J, Navarro-Llorens JM | BMC Genomics | 10.1186/s12864-019-5677-2 | 2019 | |
| Metabolism | Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin-Layer Chromatography (TLC). | Guevara G, Perera J, Navarro-Llorens JM | Methods Mol Biol | 10.1007/978-1-4939-7183-1_24 | 2017 | |
| Metabolism | Functional characterization of 3-ketosteroid 9alpha-hydroxylases in Rhodococcus ruber strain chol-4. | Guevara G, Heras LFL, Perera J, Llorens JMN | J Steroid Biochem Mol Biol | 10.1016/j.jsbmb.2017.06.011 | 2017 | |
| Enzymology | Functional differentiation of 3-ketosteroid Delta(1)-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4. | Guevara G, Fernandez de Las Heras L, Perera J, Navarro Llorens JM | Microb Cell Fact | 10.1186/s12934-017-0657-1 | 2017 | |
| Enzymology | Cholesterol to cholestenone oxidation by ChoG, the main extracellular cholesterol oxidase of Rhodococcus ruber strain Chol-4. | Fernandez de Las Heras L, Perera J, Navarro Llorens JM | J Steroid Biochem Mol Biol | 10.1016/j.jsbmb.2013.10.001 | 2013 | |
| Genetics | Draft Genome Sequence of the Steroid Degrader Rhodococcus ruber Strain Chol-4. | Fernandez de Las Heras L, Alonso S, de la Vega de Leon A, Xavier D, Perera J, Navarro Llorens JM | Genome Announc | 10.1128/genomeA.00215-13 | 2013 | |
| Metabolism | Gene cluster encoding cholate catabolism in Rhodococcus spp. | Mohn WW, Wilbrink MH, Casabon I, Stewart GR, Liu J, van der Geize R, Eltis LD | J Bacteriol | 10.1128/JB.01169-12 | 2012 | |
| Enzymology | Molecular characterization of three 3-ketosteroid-Delta(1)-dehydrogenase isoenzymes of Rhodococcus ruber strain Chol-4. | Fernandez de las Heras L, van der Geize R, Drzyzga O, Perera J, Maria Navarro Llorens J | J Steroid Biochem Mol Biol | 10.1016/j.jsbmb.2012.06.005 | 2012 | |
| Phylogeny | Morphological, physiological, and molecular characterization of a newly isolated steroid-degrading actinomycete, identified as rhodococcus ruber strain Chol-4. | Fernandez de Las Heras L, Garcia Fernandez E, Maria Navarro Llorens J, Perera J, Drzyzga O | Curr Microbiol | 10.1007/s00284-009-9474-z | 2009 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive10955.20260601.11
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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