Name: Bacillus mojavensis RO-H-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1093

Type strain:

Species: Bacillus mojavensis

Strain Designation: RO-H-1

Culture col. no.: DSM 9205, ATCC 51516, NRRL B-14698, JCM 12230, BCRC 17124, CIP 104095, IFO 15718, KCTC 3706, LMG 17797, NBRC 15718, NCIMB 13391, VTT E-011771

Strain history: CIP <- 1994, F.M. Cohan, Wesleyan Univ., Middletown, USA: strain RO-H-1

NCBI tax ID(s): 1051501 (strain), 72360 (species)

SI-ID 10680

LMG 17797, ATCC 51516, DSM 9205, IFO 15718, NRRL B-14698, CIP 104095, NCIMB 13391, KCTC 3706, NBRC 15718, BCRC 17124, CCRC 17124, CGMCC 1.3656, VTT E-011771, JCM 12230

Special Collections

Other strains from Bacillus mojavensis

Section

Bacillus mojavensis RO-H-1 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from soil.

colony-forming
Gram-positive
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus mojavensis**
	Full Scientific Name (LPSN)	Bacillus mojavensis Roberts et al. 1994

Information on morphological and physiological properties Morphology

[Ref.: #23301]	Gram stain	positive
[Ref.: #121001]	Gram stain	positive

[Ref.: #23301]	Cell length	2.0-4.0 µm

[Ref.: #23301]	Cell width	0.5-1.0 µm

[Ref.: #23301]	Cell shape	rod-shaped
[Ref.: #121001]	Cell shape	rod-shaped

[Ref.: #121001]	Motility	yes

[Ref.: #23301]	Colony size	1-2 mm
[Ref.: #23301]	Colony color	opaque
[Ref.: #23301]	Colony shape	circular
[Ref.: #23301]	Incubation period	2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #38164]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38164]

Culture medium growth

[Ref.: #38164]

Culture medium composition

expand / minimize

[Ref.: #3513]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #3513]

Culture medium growth

[Ref.: #3513]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3513]

Culture medium composition

expand / minimize

[Ref.: #121001]

Culture medium

CIP Medium 72

[Ref.: #121001]

Culture medium growth

[Ref.: #121001]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121001]

Culture medium

CIP Medium 3

[Ref.: #121001]

Culture medium growth

[Ref.: #121001]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3513]	growth	30 °C
[Ref.: #23301]	maximum	50-55 °C
[Ref.: #23301]	minimum	5-10 °C
[Ref.: #23301]	optimum	28-30 °C
[Ref.: #38164]	growth	37 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #23301]			growth	5.7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23301]

Oxygen tolerance

aerobe

[Ref.: #23301]	Spore description	ellipsoidal spores centrally or paracentrally in unswollen sporangia
[Ref.: #23301]	Type of spore	endospore
[Ref.: #23301]	Ability of spore formation	yes
[Ref.: #121001]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %94.447

	Salt		Salt conc.
[Ref.: #23301]	NaCl	growth	3	%
[Ref.: #23301]	NaCl	growth	5	%
[Ref.: #23301]	NaCl	growth	7	%
[Ref.: #23301]	NaCl	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23301]	arginine ChEBI	-	degradation
[Ref.: #23301]	casein	+	hydrolysis
[Ref.: #23301]	cellobiose ChEBI	-	builds gas from
[Ref.: #23301]	cellobiose ChEBI	+	builds acid from
[Ref.: #23301]	citrate ChEBI	+	assimilation
[Ref.: #23301]	egg yolk	-	degradation
[Ref.: #23301]	fructose ChEBI	-	builds gas from
[Ref.: #23301]	fructose ChEBI	+	builds acid from
[Ref.: #23301]	galactose ChEBI	-	builds gas from
[Ref.: #23301]	galactose ChEBI	+	builds acid from
[Ref.: #23301]	glucose ChEBI	-	builds gas from
[Ref.: #23301]	glucose ChEBI	+	builds acid from
[Ref.: #23301]	L-arabinose ChEBI	-	builds gas from
[Ref.: #23301]	L-arabinose ChEBI	+	builds acid from
[Ref.: #23301]	L-rhamnose ChEBI	-	builds gas from
[Ref.: #23301]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #23301]	lactose ChEBI	-	builds acid from
[Ref.: #23301]	lactose ChEBI	-	fermentation
[Ref.: #23301]	lecithin ChEBI	-	degradation
[Ref.: #23301]	lysine ChEBI	-	degradation
[Ref.: #23301]	maltose ChEBI	-	builds gas from
[Ref.: #23301]	maltose ChEBI	+	builds acid from
[Ref.: #23301]	mannitol ChEBI	-	builds gas from
[Ref.: #23301]	mannitol ChEBI	+	builds acid from
[Ref.: #23301]	mannose ChEBI	-	builds gas from
[Ref.: #23301]	mannose ChEBI	+	builds acid from
[Ref.: #23301]	melibiose ChEBI	-	fermentation
[Ref.: #23301]	nitrate ChEBI	+	reduction
[Ref.: #23301]	ornithine ChEBI	-	degradation
[Ref.: #23301]	phenylalanine ChEBI	-	degradation
[Ref.: #23301]	propionate ChEBI	-	assimilation
[Ref.: #23301]	ribose ChEBI	-	builds gas from
[Ref.: #23301]	ribose ChEBI	+	builds acid from
[Ref.: #23301]	salicin ChEBI	-	builds gas from
[Ref.: #23301]	salicin ChEBI	+	builds acid from
[Ref.: #23301]	sorbitol ChEBI	-	builds gas from
[Ref.: #23301]	sorbitol ChEBI	+	builds acid from
[Ref.: #23301]	starch ChEBI	+	hydrolysis
[Ref.: #23301]	sucrose ChEBI	-	builds gas from
[Ref.: #23301]	sucrose ChEBI	+	builds acid from
[Ref.: #23301]	trehalose ChEBI	-	builds gas from
[Ref.: #23301]	trehalose ChEBI	+	builds acid from
[Ref.: #23301]	tween 80 ChEBI	-	degradation
[Ref.: #23301]	tyrosine ChEBI	-	degradation
[Ref.: #23301]	urea ChEBI	-	degradation
[Ref.: #23301]	xylose ChEBI	+	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23301]	acetoin ChEBI	yes
[Ref.: #23301]	dihydroxyacetone ChEBI	no
[Ref.: #23301]	hydrogen sulfide ChEBI	no
[Ref.: #23301]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #23301]		acetoin ChEBI	+

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #23301]		catalase	+	1.11.1.6
[Ref.: #23301]		cytochrome oxidase	+	1.9.3.1

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121001]	546	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	+	-	-	+	+	+	+	+	+	+	+	+	+	-	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-	+	-	+	+	-	-	-	-	+	+	-	-	+	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	-	+	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3513]	Sample type/isolated from	soil
[Ref.: #3513]	Geographic location (country and/or sea, region)	Mojave Desert, California
[Ref.: #3513]	Country	USA
[Ref.: #3513]	Country ISO 3 Code	USA
[Ref.: #3513]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Soil, Mojave Desert
[Ref.: #67770]	Geographic location (country and/or sea, region)	California
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #121001]	Sample type/isolated from	Environment, Desert soil
[Ref.: #121001]	Geographic location (country and/or sea, region)	Mojave desert, California
[Ref.: #121001]	Country	United States of America
[Ref.: #121001]	Country ISO 3 Code	USA
[Ref.: #121001]	Continent	North America
[Ref.: #121001]	Isolation date	1987
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JN585825 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3513]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121001]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Bacillus mojavensis clone MOJ1F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF478089	403	GenBank ENA	72360 tax ID	*
[Ref.: #20218]	Bacillus mojavensis strain BCRC 17124 16S ribosomal RNA gene, partial sequence	EF433405	1468	GenBank ENA	72360 tax ID	*
[Ref.: #20218]	Bacillus mojavensis gene for 16S ribosomal RNA	AB021191	1526	GenBank ENA	72360 tax ID	*
[Ref.: #20218]	Bacillus mojavensis strain ifo 15718 16S ribosomal RNA, partial sequence	JN585825	1407	GenBank ENA	72360 tax ID	*
[Ref.: #20218]	Bacillus mojavensis gene for 16S rRNA, partial sequence, strain: NBRC 15718	AB363735	1475	GenBank ENA	72360 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Bacillus mojavensis RO-H-1 = KCTC 3706	GCA_000245335	scaffold	GenBank ENA	1051501 tax ID
[Ref.: #67770]	Bacillus mojavensis RO-H-1 = KCTC 3706 KCTC 3706(T)	GCA_000507105	contig	GenBank ENA	1051501 tax ID
[Ref.: #66792]	Bacillus mojavensis RO-H-1	GCA_014499005	scaffold	GenBank ENA	72360 tax ID	*
[Ref.: #66792]	Bacillus mojavensis RO-H-1	1051501.3	wgs	PATRIC	1051501 tax ID	*
[Ref.: #66792]	Bacillus mojavensis RO-H-1 [PRJNA227789]	1051501.5	wgs	PATRIC	1051501 tax ID	*
[Ref.: #66792]	Bacillus mojavensis RO-H-1	2545555827	draft	JGI IMG/M	1051501 tax ID	*

[Ref.: #3513]	GC-content	43 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #23301]	GC-content	43.00 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus mojavensis RO-H-1 PATRIC: 1051501.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	85.604	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.332	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	94.447	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	74.001	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.545	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	89.322	no

Availability in culture collections External links

[Ref.: #3513]

Culture collection no.

DSM 9205, ATCC 51516, NRRL B-14698, JCM 12230, BCRC 17124, CIP 104095, IFO 15718, KCTC 3706, LMG 17797, NBRC 15718, NCIMB 13391, VTT E-011771

[Ref.: #70765]

SI-ID 10680

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Whole-genome sequences of Bacillus subtilis and close relatives.	Earl AM, Eppinger M, Fricke WF, Rosovitz MJ, Rasko DA, Daugherty S, Losick R, Kolter R, Ravel J	J Bacteriol	10.1128/JB.05675-11	2012	*
Phylogeny	Bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in Thailand.	Sumpavapol P, Tongyonk L, Tanasupawat S, Chokesajjawatee N, Luxananil P, Visessanguan W	Int J Syst Evol Microbiol	10.1099/ijs.0.018879-0	2009	*
Metabolism	Acid labile ComX pheromone from Bacillus mojavensis RO-H-1.	Okada M, Yamaguchi H, Sato I, Tsuji F, Qi J, Dubnau D, Sakagami Y	Biosci Biotechnol Biochem	10.1271/bbb.70245	2007	*
Phylogeny	Bacillus mojavensis sp. nov., distinguishable from Bacillus subtilis by sexual isolation, divergence in DNA sequence, and differences in fatty acid composition.	Roberts MS, Nakamura LK, Cohan FM	Int J Syst Bacteriol	10.1099/00207713-44-2-256	1994	*

References

#3513

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 9205

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23301

MICHAEL S. ROBERTS, L. K. NAKAMURA, FREDERICK M. COHAN: Bacillus mojavensis sp. nov., Distinguishable from Bacillus subtilis by Sexual Isolation, Divergence in DNA Sequence, and Differences in Fatty Acid Composition. IJSEM 44: 256 - 264 1994 ( DOI 10.1099/00207713-44-2-256 , PubMed 8186089 )

#38164 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#70765

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10680.1 )

#121001 Collection of Institut Pasteur ; Curators of the CIP; CIP 104095
* These data were automatically processed and therefore are not curated

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