Strain identifier
BacDive ID: 1093
Type strain:
Species: Bacillus mojavensis
Strain Designation: RO-H-1
Strain history: CIP <- 1994, F.M. Cohan, Wesleyan Univ., Middletown, USA: strain RO-H-1
NCBI tax ID(s): 1051501 (strain), 72360 (species)
General
@ref: 3513
BacDive-ID: 1093
DSM-Number: 9205
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, rod-shaped, colony-forming
description: Bacillus mojavensis RO-H-1 is an aerobe, spore-forming, thermophilic bacterium that forms circular colonies and was isolated from soil.
NCBI tax id
NCBI tax id | Matching level |
---|---|
72360 | species |
1051501 | strain |
strain history
@ref | history |
---|---|
3513 | <- NRRL <- F.M. Cohan, Wesleyan Univ., Conn.; RO-H-1 |
67770 | NRRL B-14698 <-- F. M. Cohan RO-H-1. |
121001 | CIP <- 1994, F.M. Cohan, Wesleyan Univ., Middletown, USA: strain RO-H-1 |
doi: 10.13145/bacdive1093.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus mojavensis
- full scientific name: Bacillus mojavensis Roberts et al. 1994
@ref: 3513
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Bacillus
species: Bacillus mojavensis
full scientific name: Bacillus mojavensis Roberts et al. 1994 emend. Wang et al. 2007
strain designation: RO-H-1
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
23301 | positive | 2.0-4.0 µm | 0.5-1.0 µm | rod-shaped | |
121001 | positive | rod-shaped | yes |
colony morphology
- @ref: 23301
- colony size: 1-2 mm
- colony color: opaque
- colony shape: circular
- incubation period: 2 days
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
38164 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
3513 | NUTRIENT AGAR (DSMZ Medium 1) | yes | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water | https://mediadive.dsmz.de/medium/1 |
121001 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
121001 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
23301 | positive | maximum | 50-55 | thermophilic |
23301 | positive | minimum | 5-10 | psychrophilic |
23301 | positive | optimum | 28-30 | mesophilic |
38164 | positive | growth | 37 | mesophilic |
3513 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
culture pH
- @ref: 23301
- ability: positive
- type: growth
- pH: 5.7
Physiology and metabolism
oxygen tolerance
- @ref: 23301
- oxygen tolerance: aerobe
spore formation
@ref | spore description | type of spore | spore formation |
---|---|---|---|
23301 | ellipsoidal spores centrally or paracentrally in unswollen sporangia | endospore | yes |
121001 | yes |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
23301 | NaCl | positive | growth | 3 % |
23301 | NaCl | positive | growth | 5 % |
23301 | NaCl | positive | growth | 7 % |
23301 | NaCl | positive | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23301 | 17272 | propionate | - | assimilation |
23301 | 17716 | lactose | - | builds acid from |
23301 | 17057 | cellobiose | - | builds gas from |
23301 | 28757 | fructose | - | builds gas from |
23301 | 28260 | galactose | - | builds gas from |
23301 | 17234 | glucose | - | builds gas from |
23301 | 30849 | L-arabinose | - | builds gas from |
23301 | 62345 | L-rhamnose | - | builds gas from |
23301 | 17306 | maltose | - | builds gas from |
23301 | 29864 | mannitol | - | builds gas from |
23301 | 37684 | mannose | - | builds gas from |
23301 | 33942 | ribose | - | builds gas from |
23301 | 17814 | salicin | - | builds gas from |
23301 | 30911 | sorbitol | - | builds gas from |
23301 | 17992 | sucrose | - | builds gas from |
23301 | 27082 | trehalose | - | builds gas from |
23301 | 29016 | arginine | - | degradation |
23301 | egg yolk | - | degradation | |
23301 | 61995 | lecithin | - | degradation |
23301 | 25094 | lysine | - | degradation |
23301 | 18257 | ornithine | - | degradation |
23301 | 28044 | phenylalanine | - | degradation |
23301 | 53426 | tween 80 | - | degradation |
23301 | 18186 | tyrosine | - | degradation |
23301 | 16199 | urea | - | degradation |
23301 | 17716 | lactose | - | fermentation |
23301 | 28053 | melibiose | - | fermentation |
23301 | 16947 | citrate | + | assimilation |
23301 | 17057 | cellobiose | + | builds acid from |
23301 | 28757 | fructose | + | builds acid from |
23301 | 28260 | galactose | + | builds acid from |
23301 | 17234 | glucose | + | builds acid from |
23301 | 30849 | L-arabinose | + | builds acid from |
23301 | 62345 | L-rhamnose | + | builds acid from |
23301 | 17306 | maltose | + | builds acid from |
23301 | 29864 | mannitol | + | builds acid from |
23301 | 37684 | mannose | + | builds acid from |
23301 | 33942 | ribose | + | builds acid from |
23301 | 17814 | salicin | + | builds acid from |
23301 | 30911 | sorbitol | + | builds acid from |
23301 | 17992 | sucrose | + | builds acid from |
23301 | 27082 | trehalose | + | builds acid from |
23301 | 18222 | xylose | + | fermentation |
23301 | casein | + | hydrolysis | |
23301 | 28017 | starch | + | hydrolysis |
23301 | 17632 | nitrate | + | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23301 | 35581 | indole | no |
23301 | 16136 | hydrogen sulfide | no |
23301 | 16016 | dihydroxyacetone | no |
23301 | 15688 | acetoin | yes |
metabolite tests
- @ref: 23301
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
@ref | value | activity | ec |
---|---|---|---|
23301 | catalase | + | 1.11.1.6 |
23301 | cytochrome oxidase | + | 1.9.3.1 |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121001 | + | + | + | + | + | - | + | + | + | + | + | + | - | - | + | + | + | + | + | + | + | + | + | + | - | - | - | - | + | - | - | + | + | + | + | + | + | - | - | - | - | + | - | + | + | - | - | - | - | + | + | - | - | + | + | + | + | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | + | - | + | - | - | - | + | + | - | + | - | - | - | + | - | - | + | + | + | + | + | + | - | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
3513 | soil | Mojave Desert, California | USA | USA | North America | |
67770 | Soil, Mojave Desert | California | USA | USA | North America | |
121001 | Environment, Desert soil | Mojave desert, California | United States of America | USA | North America | 1987 |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_21.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_17;97_17;98_20;99_21&stattab=map
- Last taxonomy: Bacillus
- 16S sequence: JN585825
- Sequence Identity:
- Total samples: 18201
- soil counts: 3768
- aquatic counts: 2854
- animal counts: 9674
- plant counts: 1905
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
3513 | 1 | Risk group (German classification) |
121001 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Bacillus mojavensis clone MOJ1F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | AF478089 | 403 | ena | 72360 |
20218 | Bacillus mojavensis strain BCRC 17124 16S ribosomal RNA gene, partial sequence | EF433405 | 1468 | ena | 72360 |
20218 | Bacillus mojavensis gene for 16S ribosomal RNA | AB021191 | 1526 | ena | 72360 |
20218 | Bacillus mojavensis strain ifo 15718 16S ribosomal RNA, partial sequence | JN585825 | 1407 | ena | 72360 |
20218 | Bacillus mojavensis gene for 16S rRNA, partial sequence, strain: NBRC 15718 | AB363735 | 1475 | ena | 72360 |
20218 | Bacillus mojavensis strain NRRL B-14698 16S ribosomal RNA gene, partial sequence | EU138460 | 1168 | ena | 72360 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Bacillus mojavensis RO-H-1 | GCA_014499005 | scaffold | ncbi | 72360 |
66792 | Bacillus mojavensis RO-H-1 | 1051501.3 | wgs | patric | 1051501 |
66792 | Bacillus mojavensis RO-H-1 [PRJNA227789] | 1051501.5 | wgs | patric | 1051501 |
66792 | Bacillus mojavensis RO-H-1 | 2545555827 | draft | img | 1051501 |
66792 | Bacillus mojavensis RO-H-1, KCTC 3706 | 2579778565 | draft | img | 1051501 |
67770 | Bacillus mojavensis RO-H-1 = KCTC 3706 | GCA_000245335 | scaffold | ncbi | 1051501 |
67770 | Bacillus mojavensis RO-H-1 = KCTC 3706 KCTC 3706(T) | GCA_000507105 | contig | ncbi | 1051501 |
GC content
@ref | GC-content | method |
---|---|---|
3513 | 43 | thermal denaturation, midpoint method (Tm) |
23301 | 43.00 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 87.542 | no |
flagellated | yes | 88.048 | no |
gram-positive | yes | 92.898 | no |
anaerobic | no | 99.312 | no |
aerobic | yes | 92.269 | no |
halophile | yes | 87.435 | yes |
spore-forming | yes | 96.637 | yes |
thermophile | no | 97.282 | no |
glucose-util | yes | 89.332 | no |
glucose-ferment | no | 90.07 | no |
External links
@ref: 3513
culture collection no.: DSM 9205, ATCC 51516, NRRL B-14698, JCM 12230, BCRC 17124, CIP 104095, IFO 15718, KCTC 3706, LMG 17797, NBRC 15718, NCIMB 13391, VTT E-011771
straininfo link
- @ref: 70765
- straininfo: 10680
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 8186089 | Bacillus mojavensis sp. nov., distinguishable from Bacillus subtilis by sexual isolation, divergence in DNA sequence, and differences in fatty acid composition. | Roberts MS, Nakamura LK, Cohan FM | Int J Syst Bacteriol | 10.1099/00207713-44-2-256 | 1994 | Bacillus/*classification/*genetics/isolation & purification, Bacillus subtilis/classification/genetics, Base Composition, Base Sequence, DNA, Bacterial/genetics, Desert Climate, Drug Resistance, Microbial, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Polymerase Chain Reaction, Rifampin/pharmacology, Selection, Genetic, *Soil Microbiology | Genetics |
Metabolism | 17617699 | Acid labile ComX pheromone from Bacillus mojavensis RO-H-1. | Okada M, Yamaguchi H, Sato I, Tsuji F, Qi J, Dubnau D, Sakagami Y | Biosci Biotechnol Biochem | 10.1271/bbb.70245 | 2007 | Bacillus/*metabolism, Bacterial Proteins/chemistry/isolation & purification/*metabolism | Enzymology |
Phylogeny | 19933584 | Bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in Thailand. | Sumpavapol P, Tongyonk L, Tanasupawat S, Chokesajjawatee N, Luxananil P, Visessanguan W | Int J Syst Evol Microbiol | 10.1099/ijs.0.018879-0 | 2009 | Bacillus/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, *Food Microbiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/cytology, Temperature, Thailand | Biotechnology |
Genetics | 22493193 | Whole-genome sequences of Bacillus subtilis and close relatives. | Earl AM, Eppinger M, Fricke WF, Rosovitz MJ, Rasko DA, Daugherty S, Losick R, Kolter R, Ravel J | J Bacteriol | 10.1128/JB.05675-11 | 2012 | Bacillus/classification/*genetics, Chromosomes, Bacterial, DNA, Bacterial/genetics, Gene Expression Regulation, Bacterial, *Genome, Bacterial, Molecular Sequence Data | Phylogeny |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3513 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9205) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9205 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23301 | MICHAEL S. ROBERTS, L. K. NAKAMURA, FREDERICK M. COHAN | 10.1099/00207713-44-2-256 | Bacillus mojavensis sp. nov., Distinguishable from Bacillus subtilis by Sexual Isolation, Divergence in DNA Sequence, and Differences in Fatty Acid Composition | IJSEM 44: 256-264 1994 | 8186089 | |
38164 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15964 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
70765 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID10680.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121001 | Curators of the CIP | Collection of Institut Pasteur (CIP 104095) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104095 |